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48:." This is a spontaneously transformed ( immortalized ) cell line. As such, it is a very convenient research tool but caution should be used when extrapolating these results to normal cells, and even more, to normal human cells.
74:. One published research article, using MC3T3-E1 cells as a model for studying cartilage regeneration, describes some of the specific limitations of the cell line for understanding behavior in human cells.
67:. Beyond a critical density of cell adhesion motifs MC3T3 cells polarize and form stable focal adhesions. MC3T3 cells show higher proliferation and polarization on stiff substrates.
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Kodama H. A., Amagai Y., Sudo H., Kasai S., Yamamoto S. Establishment of a clonal osteogenic cell line from newborn mouse calvaria. Jpn J Oral Biol. 1981. Oct;23(4):899-901.
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A number of derivatives of this strain have been isolated to select for varying degrees of osteogenic potential, and have been widely used as model systems in
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Fernandes, Hugo; Dechering, Koen; van
Someren, Eugene; Steeghs, Ilse; Apotheker, Marion; Mentink, Anouk; van Blitterswijk, Clemens; de Boer, Jan (2010).
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The MC3T3 cell line is also used for modeling skeletal tissue regeneration because its undifferentiated state is phenotypically similar to that of an
297:"A Modular Platform for Cytocompatible Hydrogels with Tailored Mechanical Properties Based on Monolithic Matrices and Particulate Building Blocks"
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Andrée, Lea; Bertsch, Pascal; Wang, Rong; Becker, Malin; Leijten, Jeroen; Fischer, Peter; Yang, Fang; Leeuwenburgh, Sander C. G. (12 June 2023).
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biology. A standard textbook calls its MC3T3-E1 sub-line "one of the most convenient and physiologically relevant systems for study of
219:"A quantitative study of MC3T3-E1 cell adhesion, morphology and biomechanics on chitosan–collagen blend films at single cell level"
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358:"Prolotherapy agent P2G is associated with upregulation of fibroblast growth factor-2 genetic expression in vitro"
258:"Nanopatterned Adhesive, Stretchable Hydrogel to Control Ligand Spacing and Regulate Cell Spreading and Migration"
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Johnston, E; Emani, C; Kochan, A; Ghebrehawariat, K; Tyburski, J; Johnston, M; Rabago, D (2020).
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115:"Differential Response of MC3T3-E1 and Human Mesenchymal Stem Cells to Inositol Hexakisphosphate"
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Wang, Chuang; Xie, Xu-dong; Huang, Xun; Liang, Zhi-hong; Zhou, Chang-ren (August 2015).
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Deng, Jie; Zhao, Changsheng; Spatz, Joachim P.; Wei, Qiang (22 August 2017).
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Arriero, MarĂa del Mar; Ramis, Joana M.; PerellĂł, Joan; Monjo, Marta (2012).
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Bilezikian, John P., Lawrence G. Raisz, and Gideon A. Rodan.
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MC3T3 cells adopt a polygonal shape without considerable
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