26:
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DNA methylation at CpG dinucleotides, the most common DNA modification in eukaryotes, has been associated with various phenomena such as alterations in chromatin structure,
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faces tightly packed against each other. The beta sheet is composed of two long inner strands (beta2 and beta3) sandwiched by two shorter outer strands (beta1 and beta4).
150:
86:
527:
Bhattacharya SK, Ramchandani S, Cervoni N, Szyf M (1999). "A mammalian protein with specific demethylase activity for mCpG DNA".
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201:. MBD has negligible non-specific affinity for unmethylated DNA. In vitro foot-printing with the chromosomal protein
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111:
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580:"Solution structure of the methyl-CpG binding domain of human MBD1 in complex with methylated DNA"
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Ho, KL; McNae, IW; Schmiedeberg, L; Klose, RJ; Bird, AP; Walkinshaw, MD (29 February 2008).
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showed that the MBD could protect a 12 nucleotide region surrounding a methyl CpG pair.
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comprising a layer of twisted beta sheet, backed by another layer formed by the alpha1
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25:
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of ~70 residues, the methyl-CpG-binding domain (MBD), which is linked to additional
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488:"Characterization of Arabidopsis thaliana methyl-CpG-binding domain (MBD) proteins"
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to DNA, and in cases of MeCP2, MBD1 and MBD2, preferentially to methylated CpG. In
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133:
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Ohki I, Shimotake N, Fujita N, Jee J, Ikegami T, Nakao M, Shirakawa M (May 2001).
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388:"Dissection of the methyl-CpG binding domain from the chromosomal protein MeCP2"
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366:). It recognizes the hydration of the major groove at methylated sites.
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remodelling factors, leading to chromatin compaction and, consequently, to
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The structure of the MBD domain bound to methylated DNA has been solved (
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621:"MeCP2 binding to DNA depends upon hydration at methyl-CpG"
437:"Comparative study of methyl-CpG-binding domain proteins"
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This article incorporates text from the public domain
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18:
224:. Effects of DNA methylation are mediated through
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435:Roloff TC, Ropers HH, Nuber UA (January 2003).
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24:
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341:at the C terminus. These layers are both
194:that contains one or more symmetrically
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244:associated with chromatin, such as the
232:to symmetrically methylated CpGs. Such
15:
268:proteins, which recruit a variety of
7:
314:, the MBD has been shown to mediate
220:inactivation, differentiation, and
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386:Nan X, Meehan RR, Bird A (1993).
30:solution structure of human MECO2
505:10.1046/j.1365-313x.2003.01756.x
345:, with the alpha1 helix and the
486:Zemach A, Grafi G (June 2003).
1:
597:10.1016/S0092-8674(01)00324-5
318:. MBDs are also found in DNA
122:Available protein structures:
638:10.1016/j.molcel.2007.12.028
329:into an alpha/beta sandwich
316:protein-protein interactions
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184:Methyl-CpG-binding domain
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23:
349:lying parallel and the
404:10.1093/nar/21.21.4886
454:10.1186/1471-2164-4-1
272:(HDAC) complexes and
283:. The MBD of MeCP2,
541:1999Natur.397..579B
270:histone deacetylase
236:contain a specific
392:Nucleic Acids Res
264:appear to act as
260:. MBD-containing
188:molecular biology
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171:structure summary
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691:Protein domains
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398:(21): 4886–92.
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625:Molecular Cell
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337:and a hairpin
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441:BMC Genomics
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324:
218:chromosome X
212:imprinting,
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183:
181:
351:hydrophobic
343:amphipathic
320:demethylase
252:motif, the
246:bromodomain
36:Identifiers
370:References
347:beta sheet
281:repression
266:structural
258:PHD finger
254:SET domain
214:transposon
196:methylated
134:structures
677:IPR001739
331:structure
299:mediates
274:chromatin
256:, or the
190:binds to
186:(MBD) in
80:IPR001739
685:Category
673:InterPro
657:18313390
606:11371345
557:10050851
514:12787239
473:12529184
447:(1): 1.
325:The MBD
262:proteins
234:proteins
226:proteins
151:RCSB PDB
75:InterPro
565:4408031
537:Bibcode
492:Plant J
422:8177735
301:binding
250:AT hook
242:domains
210:genomic
112:cd00122
55:PF01429
655:
604:
563:
555:
529:Nature
512:
471:
464:149351
461:
420:
413:311401
410:
312:SETDB1
248:, the
238:domain
222:cancer
166:PDBsum
140:
130:
100:SUPFAM
68:CL0081
41:Symbol
561:S2CID
335:helix
327:folds
305:human
228:that
203:MeCP2
96:SCOPe
87:SCOP2
671:and
669:Pfam
653:PMID
602:PMID
584:Cell
553:PMID
510:PMID
469:PMID
418:PMID
363:3C2I
339:loop
310:and
308:MBD3
297:BAZ2
295:and
293:MBD4
289:MBD2
285:MBD1
230:bind
216:and
199:CpGs
182:The
159:PDBj
155:PDBe
138:ECOD
128:Pfam
92:1qk9
64:clan
62:Pfam
50:Pfam
643:hdl
633:doi
592:doi
588:105
545:doi
533:397
500:doi
459:PMC
449:doi
408:PMC
400:doi
359:PDB
192:DNA
146:PDB
107:CDD
44:MBD
19:MBD
687::
675::
651:.
641:.
629:29
627:.
623:.
600:.
586:.
582:.
559:.
551:.
543:.
531:.
508:.
496:34
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467:.
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396:21
394:.
390:.
378:^
361::
322:.
291:,
287:,
157:;
153:;
136:/
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659:.
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635::
608:.
594::
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547::
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516:.
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451::
445:4
424:.
402::
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
