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According to the intended yield, this basic principle can be adapted to various types and sizes of bioreactors. The cultivation chamber can, for example, consist of a column, a tube, or exchangeable plastic bags.
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stage by continuous mechanical disruption, e.g. by using rotating blades. Once the density of the culture has reached a certain threshold, the lack of nutrients and the increasing concentration of
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have already been produced using moss bioreactors. Ideally, the recombinant protein can be directly purified from the culture medium. One example for this production method is
470:"Enhanced recovery of a secreted recombinant human growth factor using stabilizing additives and by co-expression of human serum albumin in the moss Physcomitrella patens"
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The moss bioreactor is used to cultivate moss in a suspension culture in agitated, and aerated liquid medium. The culture is kept under lighting with temperature and
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Büttner-Mainik, A.; Parsons, J.; Jérôme, H.; Hartmann, A.; Lamer, S.; Schaaf, A.; Schlosser, A.; Zipfel, P. F.; Reski, R.; Decker, E. L. (April 2011).
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were developed in the 1990s to comply with the safety standards regarding the handling of genetically modified organisms and to gain sufficient
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Hohe, A.; Reski, R. (June 2005). "Control of growth and differentiation of bioreactor cultures of
Physcomitrella by environmental parameters".
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551:"Homepage of Greenovation: Approval to begin a Phase I clinical trial in Europe for moss-aGal (agalsidase) produced in Mossbioreactors"
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Decker, Eva L.; Reski, Ralf (2008). "Current achievements in the production of complex biopharmaceuticals with moss bioreactors".
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229:
213:. Biologically active recombinant factor H was produced in a moss bioreactor for the first time in 2011. The enzyme
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293:(2005). "From axenic spore germination to molecular farming: one century of bryophyte in vitro culture".
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156:. At this point the culture has to be diluted with fresh medium if it is intended for further use.
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330:"Production of a heterologous protein in bioreactor cultures of fully differentiated moss plants"
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511:"Production of biologically active recombinant human factor H in Physcomitrella"
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Homepage of greenovation GmbH, showing various types of moss bioreactors:
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in the medium triggers the differentiation of the protonema to the adult
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221:. It will be tested as enzyme replacement therapy in the treatment of
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Photobioreactor used for the cultivation and propagation of mosses
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now is allowed to be produced in moss bioreactors by the German
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for research purposes since the beginning of the 20th century.
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Reski, Ralf; Parsons, Juliana; Decker, Eva L. (October 2015).
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Decker, E. L.; Reski, R. (April 2004). "The moss bioreactor".
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To ensure a maximum growth rate, the moss is kept at the
573:"Moss-made pharmaceuticals: from bench to bedside"
205:are associated with human diseases such as severe
219:Federal Institute for Drugs and Medical Devices
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328:Reutter, K.; Reski, R. (September 1996).
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468:Baur, A.; Reski, R.; Gorr, G. (2005).
337:Plant Tissue Culture and Biotechnology
68:moss bioreactors are used to multiply
435:Bioprocess and Biosystems Engineering
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619:Greenovation gelingt der Durchbruch
363:Plant Cell, Tissue and Organ Culture
14:
528:10.1111/j.1467-7652.2010.00552.x
487:10.1111/j.1467-7652.2005.00127.x
390:Current Opinion in Plant Biology
181:Production of biopharmaceuticals
201:. Defects in the corresponding
141:needed for growth of the moss.
1:
343:(3): 142–147. Archived from
167:In this moss bioreactor the
87:organism that has been kept
577:Plant Biotechnology Journal
515:Plant Biotechnology Journal
474:Plant Biotechnology Journal
113:for experimental purposes.
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447:10.1007/s00449-007-0151-y
420:November 9, 2011, at the
402:10.1016/j.pbi.2004.01.002
375:10.1007/s11240-004-6656-z
307:10.1007/s00299-004-0894-8
125:value held constant. The
52:. It is usually used in
232:was completed in 2017.
23:A moss bioreactor with
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83:Moss is a very frugal
76:consortium to monitor
56:for the production of
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270:Physcomitrella patens
197:is part of the human
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106:Physcomitrella patens
66:environmental science
26:Physcomitrella patens
22:
117:Functional principle
215:alpha-galactosidase
58:recombinant protein
626:2018-01-11 at the
621:Portal goingpublic
295:Plant Cell Reports
187:biopharmaceuticals
178:
30:
589:10.1111/pbi.12401
263:Sphagnum palustre
252:Molecular farming
211:retinal disorders
199:complement system
173:Sphagnum palustre
54:molecular farming
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583:(8): 1191–1198.
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85:photoautotrophic
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257:Photobioreactor
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96:The first moss
38:photobioreactor
34:moss bioreactor
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350:on 2011-10-04.
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133:—contains all
131:minimal medium
127:culture medium
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102:model organism
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650:Bioreactors
154:gametophyte
98:bioreactors
70:peat mosses
46:propagation
42:cultivation
639:Categories
556:18 October
441:(1): 3–9.
289:Hohe, A.;
277:References
247:Bioreactor
62:transgenic
291:Reski, R.
169:peat moss
146:protonema
135:nutrients
129:—often a
74:Mossclone
64:moss. In
624:Archived
607:26011014
537:20723134
496:17129315
455:17701058
418:Archived
315:15558285
236:See also
195:molecule
191:factor H
185:Various
139:minerals
100:for the
90:in vitro
598:4736463
230:phase 1
193:: this
111:biomass
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225:. The
207:kidney
60:using
50:mosses
348:(PDF)
333:(PDF)
36:is a
603:PMID
558:2015
533:PMID
492:PMID
451:PMID
311:PMID
209:and
203:gene
137:and
44:and
593:PMC
585:doi
523:doi
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123:pH
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