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utilization, so the intensity of the color produced can be used as a measure of the enzymatic rate.
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Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it is unable to act as an
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activity. This compound is normally colorless. However, if β-galactosidase is present, it
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492:(IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.
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461:. The latter compound has a yellow color that can be used to check for
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484:. Without another lactose analog that can act as an inducer, such as
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assay (at 420 nm wavelength). β-Galactosidase is required for
405:
Except where otherwise noted, data are given for materials in their
236:
226:
191:
2-Nitrophenylgalactoside, Ortho-Nitrophenyl-β-D-Galactopyranoside
498:
17:
494:
182:)-2-(Hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
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598:
565:
532:
48:. Unsourced material may be challenged and removed.
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8:
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495:
266:
119:
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108:Learn how and when to remove this message
732:
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741:"2-Nitrophenyl β-D-galactopyranoside"
7:
46:adding citations to reliable sources
293:
14:
57:"Ortho-Nitrophenyl-β-galactoside"
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372:
129:
22:
409:(at 25 °C , 100 kPa).
33:needs additional citations for
381:
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345:O=()C1=CC=CC=C1O2O(CO)(O)(O)2O
1:
124:-Nitrophenyl-β-galactoside
793:
428:-Nitrophenyl-β-galactoside
403:
353:
333:
196:
188:
156:
142:
137:
128:
777:Nitrobenzene derivatives
490:-1-thiogalactopyranoside
282:2-nitrophenylgalactoside
688:Anthraquinone glycoside
465:activity by means of a
453:the ONPG molecule into
158:Systematic IUPAC name
673:Cyanogenic glycoside
42:improve this article
698:Flavonoid glycoside
651:Alcoholic glycoside
399: g·mol
125:
693:Coumarin glycoside
683:Phenolic glycoside
440:spectrophotometric
413:Infobox references
151:-galactopyranoside
120:
723:
722:
710:Steviol glycoside
656:Cardiac glycoside
557:C-glycosidic bond
552:S-glycosidic bond
547:N-glycosidic bond
542:O-glycosidic bond
489:
459:ortho-nitrophenol
445:for detection of
421:Chemical compound
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238:Interactive image
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745:sigmaaldrich.com
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361:Chemical formula
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147:2-Nitrophenyl β-
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739:
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447:β-galactosidase
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12:
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5:
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407:standard state
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248:Abbreviations
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2:
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775:
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769:
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746:
742:
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726:
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715:Thioglycoside
713:
711:
708:
706:
703:
699:
696:
694:
691:
689:
686:
685:
684:
681:
679:
678:Glycosylamine
676:
674:
671:
667:
664:
662:
661:Bufadienolide
659:
658:
657:
654:
652:
649:
648:
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640:
634:
631:
629:
626:
624:
621:
619:
616:
614:
611:
609:
606:
605:
603:
601:
597:
591:
590:1,6-Glycoside
588:
586:
585:1,4-Glycoside
583:
581:
578:
576:
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568:
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558:
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531:
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408:
402:
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258:ECHA InfoCard
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98:February 2022
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59: –
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53:Find sources:
47:
43:
37:
36:
31:This article
29:
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20:
19:
16:
772:Galactosides
748:. Retrieved
744:
735:
486:isopropyl β-
475:
467:colorimetric
436:colorimetric
431:
425:
424:
423:
197:Identifiers
189:Other names
179:
175:
171:
167:
163:
121:
104:
95:
85:
78:
71:
64:
52:
40:Please help
35:verification
32:
15:
666:Cardenolide
623:Glucuronide
613:Galactoside
580:β-Glycoside
575:α-Glycoside
354:Properties
264:100.006.106
766:Categories
727:References
628:Rhamnoside
608:Fructoside
526:Glycosides
482:lac operon
451:hydrolyzes
392:Molar mass
324:8RFX6AT9WP
225:3D model (
204:CAS Number
144:IUPAC name
68:newspapers
750:14 August
618:Glucoside
455:galactose
443:substrate
643:Aglycone
633:Riboside
567:Geometry
480:for the
214:369-07-3
705:Saponin
600:Glycone
478:inducer
471:lactose
434:) is a
397:301.251
291:PubChem
82:scholar
463:enzyme
338:SMILES
304:123646
138:Names
84:
77:
70:
63:
55:
426:ortho
251:ONPG
227:JSmol
122:ortho
89:JSTOR
75:books
752:2023
534:Bond
457:and
438:and
432:ONPG
315:UNII
276:MeSH
61:news
294:CID
44:by
768::
743:.
376:15
370:12
178:,6
174:,5
170:,4
166:,3
162:(2
754:.
518:e
511:t
504:v
488:D
430:(
385:8
382:O
379:N
373:H
367:C
229:)
180:S
176:R
172:S
168:R
164:R
149:D
111:)
105:(
100:)
96:(
86:·
79:·
72:·
65:·
38:.
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