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Acid-fastness

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Fungal yeast forms are inconsistently stained with Acid-fast stain which is considered a narrow spectrum stain for fungi. In a study on acid-fastness of fungi, 60% of blastomyces and 47% of histoplasma showed positive cytoplasmic staining of the yeast-like cells, and Cryptococcus or candida did not
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Acid-fast organisms are difficult to characterize using standard microbiological techniques, though they can be stained using concentrated dyes, particularly when the staining process is combined with heat. Some, such as
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Very few structures are acid-fast; this makes staining for acid-fastness particularly useful in diagnosis. The following are notable examples of structures which are acid-fast or modified acid-fast:
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procedures. Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name
689: 449:" stain irregularly with ZN stain but emanate bright red fluorescence under green light, and can aid detection in moderately heavy backgrounds or with scarce hooklets. 120:, but they do not take the crystal violet well and thus appear light purple, which can still potentially result in an incorrect gram negative identification. 2116: 102:, which is responsible for the staining pattern of poor absorption followed by high retention. Some bacteria may also be partially acid-fast, such as 803: 2193: 1171: 832: 756: 1477: 1217: 1185: 910: 717:"PROPOSAL OF A SIMPLIFIED TECHNIQUE FOR STAINING BACTERIAL SPORES WITHOUT APPLYING HEAT – SUCCESSFUL MODIFICATION OF MOELLER'S METHOD" 520: 1817: 1123: 1467: 1394: 127:, in which the acid-fast species are stained bright red and stand out clearly against a blue background. Another method is the 1919: 716: 536:
Reynolds, Jackie; Moyes, Rita B.; Breakwell, Donald P. (November 2009). "Differential staining of bacteria: acid fast stain".
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Wages ds, Wear dJ. acid-fastness of fungi in blastomycosis and histoplasmosis. Arch Pathol Lab Med 1982; 106:440-41.
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The mechanisms of acid-fastness vary by species although the most well-known example is in the genus
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Clavel A, Varea M, Doiz O, LĂłpez L, QuĂ­lez J, Castillo FJ, Rubio C, GĂłmez-Lus R (1999).
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For color blind people (or in backgrounds where detecting red bacteria is difficult),
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Dorner, W. 1926. Un procédé simple pour la colouration des spores. Le Lait 6:8–12.
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M. Hayama; K. Oana; T. Kozakai; S. Umeda; J. Fujimoto; H. Ota; Y. Kawakami (2007).
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The most common staining technique used to identify acid-fast bacteria is the
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Schaeffer AB, Fulton M (1933). "A simplified method of staining endospores".
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Theory and Practice of Histological Techniques, John D Bancroft, 6th ed, p314
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Madison B (2001). "Application of stains in clinical microbiology".
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stain, and very rare staining was seen in Coccidioides endospores.
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Ortega YR, Sterling CR, Gilman RH, Cama VA, DĂ­az F (May 1993).
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Various bacterial spore staining techniques using Kenyon e.g.
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Garcia LS, Bruckner DA, Brewer TC, Shimizu RY (July 1983).
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Physical property of certain bacterial and eukaryotic cells
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Ng E, Markell EK, Fleming RL, Fried M (September 1984).
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Dorner's method (acid alcohol decolorizer) without the
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(1993). 465: 439:eggs don't (can be used to distinguish) 2117:Jaswant Singh–Bhattacharji (JSB) stain 358:Neurons in layer 5 of cerebral cortex 280:do NOT have); not to be confused with 390:parasites in faecal matter, such as: 7: 1780: 724:European Journal of Medical Research 286:, which is a non-acid-fast genus of 155:(classic and modified bleach types) 2194:Grocott's methenamine silver stain 194:modification (decolorize by water) 147:Some acid-fast staining techniques 46:is a physical property of certain 25: 1827:Microbial and histological stains 538:Current Protocols in Microbiology 371:Nuclear inclusion bodies seen in 348:Certain cellular inclusions e.g. 139:using specific fluorescent dyes ( 1779: 1770: 1769: 611:"Acid fast / Auramine-rhodamine" 1468:Bacterial cellular morphologies 1092:10.1128/JCM.37.5.1561-1563.1999 546:10.1002/9780471729259.mca03hs15 39:(stained red) in tissue (blue). 511:Ryan KJ; Ray CG, eds. (2004). 360:neuronal ceroid lipofuscinosis 1: 1183:Ellis & Zabrowarny method 1002:10.1128/JCM.20.3.384-386.1984 953:10.1128/JCM.18.1.185-190.1983 867:Journal of Clinical Pathology 692:. 1 June 2012. Archived from 515:(4th ed.). McGraw Hill. 513:Sherris Medical Microbiology 243:Notable acid-fast structures 1052:10.1056/NEJM199305063281804 668:10.1126/science.77.1990.194 540:. Appendix 3: Appendix 3H. 2279: 1920:Periodic acid–Schiff stain 1711:Bacteria (classifications) 1433:Primary nutritional groups 804:"Stainsfile – Fite Faraco" 690:"Endospore Stain Protocol" 226:Ellis and Zabrowarny stain 135:can also be visualized by 116:, can be stained with the 36:Mycobacterium tuberculosis 1765: 1124:"Dako Products – Agilent" 228:(no phenol/carbolic acid) 2062:Light Green SF yellowish 2053:Masson's trichrome stain 2012:Auramine–rhodamine stain 1587:Bacterial outer membrane 1166:Online protocol examples 833:"Stainsfile – Wade Fite" 232:Auramine-rhodamine stain 141:auramine-rhodamine stain 418:A few other parasites: 410:Cyclospora cayetanensis 167:can be substituted for 137:fluorescence microscopy 90:. The acid-fastness of 59:sub-cellular structures 2179:Schaeffer–Fulton stain 2149:Gömöri trichrome stain 1582:Gram-negative bacteria 1561:Gram-positive bacteria 395:Cryptosporidium parvum 335:Bacterial spores, see 201:surfactants type NP-7 40: 2154:Luxol fast blue stain 2029:Auramine phenol stain 1437:Substrate preference 615:Pathologyoutlines.com 237:Auramine phenol stain 33: 2199:Warthin–Starry stain 2072:Phosphomolybdic acid 1418:Microbial metabolism 879:10.1136/jcp.46.6.559 445:, especially their " 433:eggs stain well but 2215:Tissue stainability 1987:Ziehl–Neelsen stain 1847:Perls Prussian blue 1668:Non-motile bacteria 1264:Pathogenic bacteria 757:"Stainsfile – Fite" 660:1933Sci....77..194S 343:Legionella micdadei 153:Ziehl–Neelsen stain 125:Ziehl–Neelsen stain 94:is due to the high 2189:Bielschowsky stain 2091:Van Gieson's stain 2057:Lillie's trichrome 1597:Lipopolysaccharide 1188:2006-01-04 at the 990:J. Clin. Microbiol 941:J. Clin. Microbiol 583:Abe C (2003). "". 379:Bismuth poisoning. 65:during laboratory 57:, as well as some 41: 2253:Acid-fast bacilli 2240: 2239: 2041:Connective tissue 1793: 1792: 1692: 1691: 1638:Bacterial capsule 1604:Periplasmic space 1571:Lipoteichoic acid 1456: 1455: 1428:Microbial ecology 1423:Nitrogen fixation 1192:for staining AFB. 487:10.1080/714028138 475:Biotech Histochem 310:(an actinomycete) 214:Fite-Faraco stain 98:content of their 18:Acid-fast bacilli 16:(Redirected from 2270: 2067:Biebrich scarlet 1820: 1813: 1806: 1797: 1783: 1782: 1773: 1772: 1721:Former groupings 1515: 1366:Human microbiome 1289: 1220: 1213: 1206: 1197: 1159: 1156: 1150: 1149: 1147: 1145: 1139: 1133:. Archived from 1128: 1120: 1114: 1113: 1103: 1080:J Clin Microbiol 1071: 1065: 1064: 1054: 1030: 1024: 1023: 1013: 981: 975: 974: 964: 932: 926: 925: 923: 922: 913:. Archived from 907: 901: 900: 890: 858: 852: 851: 849: 848: 839:. Archived from 829: 823: 822: 820: 819: 810:. Archived from 800: 794: 793: 782: 776: 775: 773: 772: 763:. Archived from 753: 747: 746: 744: 742: 721: 712: 706: 705: 703: 701: 686: 680: 679: 643: 637: 634: 628: 625: 619: 618: 607: 601: 600: 580: 574: 573: 533: 527: 526: 508: 499: 498: 470: 386:Oocysts of some 353:inclusion bodies 199:polyglycol ether 192:Schaeffer–Fulton 186:Moeller's method 143:, for example). 21: 2278: 2277: 2273: 2272: 2271: 2269: 2268: 2267: 2243: 2242: 2241: 2236: 2210: 2095: 2049:trichrome stain 2035: 1947: 1924: 1896: 1851: 1829: 1824: 1794: 1789: 1761: 1716:Bacterial phyla 1700: 1688: 1672: 1630: 1624: 1615:Arabinogalactan 1520: 1504: 1452: 1349: 1293: 1281: 1273: 1259:Lysogenic cycle 1240: 1233: 1224: 1190:Wayback Machine 1168: 1163: 1162: 1157: 1153: 1143: 1141: 1140:on 4 March 2016 1137: 1126: 1122: 1121: 1117: 1073: 1072: 1068: 1045:(18): 1308–12. 1039:N. Engl. J. Med 1032: 1031: 1027: 983: 982: 978: 934: 933: 929: 920: 918: 909: 908: 904: 860: 859: 855: 846: 844: 837:stainsfile.info 831: 830: 826: 817: 815: 808:stainsfile.info 802: 801: 797: 784: 783: 779: 770: 768: 761:stainsfile.info 755: 754: 750: 740: 738: 719: 714: 713: 709: 699: 697: 688: 687: 683: 645: 644: 640: 635: 631: 626: 622: 609: 608: 604: 582: 581: 577: 535: 534: 530: 523: 510: 509: 502: 472: 471: 467: 462: 430:Taenia saginata 257:M. tuberculosis 245: 219:Wade Fite stain 149: 28: 23: 22: 15: 12: 11: 5: 2276: 2274: 2266: 2265: 2260: 2255: 2245: 2244: 2238: 2237: 2235: 2234: 2229: 2224: 2218: 2216: 2212: 2211: 2209: 2208: 2206:Wright's stain 2203: 2202: 2201: 2196: 2191: 2181: 2176: 2171: 2166: 2161: 2156: 2151: 2146: 2141: 2136: 2135: 2134: 2129: 2119: 2114: 2109: 2103: 2101: 2097: 2096: 2094: 2093: 2087: 2086: 2085: 2084: 2079: 2077:Fast Green FCF 2074: 2069: 2064: 2045: 2043: 2037: 2036: 2034: 2033: 2032: 2031: 2026: 2025: 2024: 2019: 2009: 2008: 2007: 2005:Methylene blue 2002: 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1342: 1337: 1335:Microaerophile 1332: 1331: 1330: 1325: 1315: 1314: 1313: 1308: 1297: 1295: 1286: 1275: 1274: 1272: 1271: 1266: 1261: 1256: 1251: 1245: 1243: 1235: 1234: 1225: 1223: 1222: 1215: 1208: 1200: 1194: 1193: 1179: 1167: 1164: 1161: 1160: 1151: 1115: 1066: 1025: 976: 927: 902: 873:(6): 559–560. 853: 824: 795: 777: 748: 730:(8): 356–359. 707: 696:on 1 June 2012 681: 638: 629: 620: 602: 575: 528: 521: 500: 464: 463: 461: 458: 457: 456: 452: 451: 450: 440: 426: 416: 415: 414: 406: 403:Isospora belli 399: 384: 383: 382: 381: 380: 377: 375:Lead poisoning 369: 368: 367: 364:Batten disease 346: 339: 333: 327: 326: 325: 318: 311: 303: 296: 271: 244: 241: 240: 239: 234: 229: 223: 222: 221: 216: 206: 205: 204: 203: 202: 195: 188: 177:methylene blue 169:carbol fuchsin 161: 156: 148: 145: 129:Kinyoun method 26: 24: 14: 13: 10: 9: 6: 4: 3: 2: 2275: 2264: 2261: 2259: 2256: 2254: 2251: 2250: 2248: 2233: 2230: 2228: 2225: 2223: 2220: 2219: 2217: 2213: 2207: 2204: 2200: 2197: 2195: 2192: 2190: 2187: 2186: 2185: 2182: 2180: 2177: 2175: 2172: 2170: 2169:Movat's stain 2167: 2165: 2164:Moeller stain 2162: 2160: 2157: 2155: 2152: 2150: 2147: 2145: 2142: 2140: 2139:Janus Green B 2137: 2133: 2130: 2128: 2125: 2124: 2123: 2122:H&E stain 2120: 2118: 2115: 2113: 2110: 2108: 2107:Cresyl violet 2105: 2104: 2102: 2098: 2092: 2089: 2088: 2083: 2080: 2078: 2075: 2073: 2070: 2068: 2065: 2063: 2060: 2059: 2058: 2054: 2050: 2047: 2046: 2044: 2042: 2038: 2030: 2027: 2023: 2020: 2018: 2015: 2014: 2013: 2010: 2006: 2003: 2001: 1997: 1994: 1993: 1992: 1991:Kinyoun stain 1988: 1985: 1984: 1983: 1980: 1976: 1973: 1971: 1967: 1966:Methyl violet 1964: 1963: 1962: 1959: 1958: 1956: 1954: 1950: 1944: 1941: 1939: 1936: 1935: 1933: 1931: 1927: 1921: 1918: 1916: 1913: 1911: 1908: 1907: 1905: 1903: 1902:Carbohydrates 1899: 1891: 1890:Sudan Black B 1888: 1886: 1883: 1881: 1878: 1876: 1873: 1871: 1868: 1867: 1866: 1863: 1862: 1860: 1858: 1854: 1848: 1845: 1844: 1842: 1840: 1836: 1832: 1828: 1821: 1816: 1814: 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695: 691: 685: 682: 677: 673: 669: 665: 661: 657: 654:(1990): 194. 653: 649: 642: 639: 633: 630: 624: 621: 616: 612: 606: 603: 598: 594: 591:(8): 541–51. 590: 586: 579: 576: 571: 567: 563: 559: 555: 551: 547: 543: 539: 532: 529: 524: 522:0-8385-8529-9 518: 514: 507: 505: 501: 496: 492: 488: 484: 481:(3): 119–25. 480: 476: 469: 466: 459: 453: 448: 444: 443:Hydatid cysts 441: 438: 437: 436:Taenia solium 432: 431: 427: 425: 424: 420: 419: 417: 412: 411: 407: 405: 404: 400: 397: 396: 392: 391: 389: 385: 378: 376: 373: 372: 370: 365: 361: 357: 356: 354: 350: 349: 347: 345: 344: 340: 338: 334: 332: 328: 324: 323: 319: 317: 316: 312: 309: 308: 304: 302: 301: 297: 294: 291: 290: 289: 285: 284: 279: 275: 274:Actinomycetes 272: 270: 269:Mycobacterium 267:and atypical 266: 262: 258: 254: 250: 249: 248: 242: 238: 235: 233: 230: 227: 224: 220: 217: 215: 212: 211: 210: 207: 200: 196: 193: 189: 187: 184: 183: 181: 180: 178: 174: 170: 166: 165:Victoria blue 162: 160: 159:Kinyoun stain 157: 154: 151: 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Index

Acid-fast bacilli

Mycobacterium tuberculosis
bacterial
eukaryotic
cells
sub-cellular structures
acids
staining
Mycobacterium
tuberculosis
leprosy
mycolic acid
cell walls
Nocardia
Gram stain
Ziehl–Neelsen stain
Kinyoun method
fluorescence microscopy
auramine-rhodamine stain
Ziehl–Neelsen stain
Kinyoun stain
Victoria blue
carbol fuchsin
picric acid
methylene blue
Moeller's method
Schaeffer–Fulton
polyglycol ether
Fite stain

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