925:
913:
75:
25:
135:
The basic unit of ABPP is the probe, which typically consists of two elements: a reactive group (RG, sometimes called a "warhead") and a tag. Additionally, some probes may contain a binding group which enhances selectivity. The reactive group usually contains a specially designed
848:
236:(multidimensional protein identification technology) is especially useful for profiling inhibitor selectivity as the potency of an inhibitor can be tested against hundreds of targets simultaneously.
196:
A major advantage of ABPP is the ability to monitor the availability of the enzyme active site directly, rather than being limited to protein or mRNA abundance. With classes of enzymes such as the
518:
Abuelyaman AS, Hudig D, Woodard SL, Powers JC (1994). "Fluorescent derivatives of diphenyl phosphonate esters: synthesis and use in the inhibition and cellular localization of serine proteases".
483:
Kam CM, Abuelyaman AS, Li Z, Hudig D, Powers JC (1993). "Biotinylated isocoumarins, new inhibitors and reagents for detection, localization, and isolation of serine proteases".
43:
843:
287:
Berger AB, Vitorino PM, Bogyo M (2004). "Activity-based protein profiling: applications to biomarker discovery, in vivo imaging and drug discovery".
330:
Speers AE, Adam GC, Cravatt BF (April 2003). "Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne cycloaddition".
885:
570:
181:
61:
217:
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ABPP using probes with different fluorophores in the same lane to simultaneously profile differences in enzyme activities
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superfamily. In this probe the fluorophosphonate is the reactive group (RG) as it binds irreversibly to the active-site
807:
208:, this technique offers a valuable advantage over traditional techniques that rely on abundance rather than activity.
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enabling the identification of hundreds of active enzymes from a single sample. This technique, known as
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technology that uses chemical probes that react with mechanistically related classes of enzymes.
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will not react with an activity-based probe. The tag may be either a reporter such as a
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Proceedings of the
National Academy of Sciences of the United States of America
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Proceedings of the
National Academy of Sciences of the United States of America
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737:
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124:
391:
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Matrix-assisted laser desorption ionization-time of flight mass spectrometer
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450:
101:
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that often interact with endogenous inhibitors or that exist as inactive
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Saghatelian A, Jessani N, Joseph A, Humphrey M, Cravatt BF (July 2004).
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426:"Activity-based probes for the proteomic profiling of metalloproteases"
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ABPP were first reported in the 1990s in the study of proteases.
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18:
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367:"Activity-based protein profiling: the serine hydrolases"
85:(FP-Rhodamine) activity-based probe for profiling of the
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may be too technical for most readers to understand
365:Liu Y, Patricelli MP, Cravatt BF (December 1999).
212:Multidimensional protein identification technology
564:
8:
228:In recent years ABPP has been combined with
844:Matrix-assisted laser desorption ionization
912:
571:
557:
549:
459:
449:
400:
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62:Learn how and when to remove this message
46:, without removing the technical details.
332:Journal of the American Chemical Society
279:
16:Analytical technique to study proteins
886:European Molecular Biology Laboratory
44:make it understandable to non-experts
7:
289:American Journal of Pharmacogenomics
14:
182:Huisgen 1,3-dipolar cycloaddition
924:
923:
911:
301:10.2165/00129785-200404060-00004
117:activity-based protein profiling
23:
864:Chromosome conformation capture
1:
892:National Institutes of Health
168:or an affinity label such as
162:post-translationally modified
808:Structure-based drug design
983:
907:
898:Wellcome Sanger Institute
113:Activity-based proteomics
854:Microfluidic-based tools
699:Human Connectome Project
631:Human Microbiome Project
392:10.1073/pnas.96.26.14694
230:tandem mass spectrometry
839:Electrospray ionization
711:Human Epigenome Project
451:10.1073/pnas.0402784101
104:, a fluorophore for in-
880:DNA Data Bank of Japan
796:Human proteome project
599:Computational genomics
520:Bioconjugate Chemistry
485:Bioconjugate Chemistry
225:
156:. An enzyme that is
109:
859:Isotope affinity tags
813:Expression proteomics
219:
77:
619:Human Genome Project
604:Comparative genomics
829:2-D electrophoresis
803:Call-map proteomics
661:Structural genomics
648:Population genomics
609:Functional genomics
532:10.1021/bc00029a004
497:10.1021/bc00024a021
442:2004PNAS..10110000S
383:1999PNAS...9614694L
258:Related inhibitors
783:Structural biology
594:Cognitive genomics
226:
123:) is a functional
110:
967:Mass spectrometry
939:
938:
834:Mass spectrometer
643:Personal genomics
344:10.1021/ja034490h
249:Mass spectrometry
198:serine hydrolases
180:for use with the
98:serine hydrolases
79:Fluorophosphonate
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758:Pharmacogenomics
753:Pharmacogenetics
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202:metalloproteases
87:serine hydrolase
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27:
26:
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962:Protein methods
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773:Transcriptomics
763:Systems biology
748:Paleopolyploidy
684:Cheminformatics
665:
582:
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517:
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477:
436:(27): 10000–5.
423:
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377:(26): 14694–9.
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329:
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286:
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269:Chemoproteomics
245:
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186:click chemistry
184:(also known as
148:residue in the
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100:and the tag is
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40:help improve it
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822:Research tools
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768:Toxicogenomics
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671:Bioinformatics
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614:Genome project
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338:(16): 4686–7.
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108:visualization.
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873:Organizations
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743:Nutrigenomics
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689:Chemogenomics
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295:(6): 371–81.
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152:of an active
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144:-linked to a
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140:that becomes
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32:This article
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21:
20:
928:
916:
738:Microbiomics
733:Metabolomics
694:Connectomics
653:
626:Metagenomics
526:(5): 400–5.
523:
519:
513:
491:(6): 560–7.
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146:nucleophilic
138:electrophile
134:
120:
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112:
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58:
49:
33:
706:Epigenomics
638:Pangenomics
234:ABPP-MudPIT
166:fluorophore
150:active site
131:Description
94:nucleophile
52:August 2014
952:Proteomics
946:Categories
791:Proteomics
728:Lipidomics
723:Immunomics
275:References
254:Proteomics
192:Advantages
142:covalently
718:Glycomics
158:inhibited
125:proteomic
102:rhodamine
83:rhodamine
957:Genomics
930:Category
656:genomics
580:Genomics
470:15220480
411:10611275
352:12696868
317:18637390
309:15651898
243:See also
206:zymogens
679:Biochip
540:7849068
505:8305526
438:Bibcode
379:Bibcode
38:Please
587:Fields
538:
503:
468:
461:454150
458:
409:
399:
350:
315:
307:
174:alkyne
172:or an
170:biotin
154:enzyme
91:serine
894:(USA)
654:Socio
402:24710
313:S2CID
178:azide
115:, or
918:List
900:(UK)
888:(EU)
882:(JP)
536:PMID
501:PMID
466:PMID
407:PMID
348:PMID
305:PMID
264:DIFP
262:and
260:MAFP
200:and
160:or
121:ABPP
528:doi
493:doi
456:PMC
446:doi
434:101
397:PMC
387:doi
340:doi
336:125
297:doi
222:gel
220:In-
188:).
176:or
106:gel
96:of
42:to
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293:4
119:(
81:-
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59:(
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36:.
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
