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Blood smear

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From the thick smear, an experienced microscopist can detect all parasites they encounter. Microscopic diagnosis can be difficult because the early trophozoites ("ring form") of all four species look identical and it is never possible to diagnose species on the basis of a single ring form; species
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allow the microscopist to screen a larger volume of blood and are about eleven times more sensitive than the thin film, so picking up low levels of infection is easier on the thick film, but the appearance of the parasite is much more distorted and therefore distinguishing between the different
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to disperse the blood over the slide's length. The aim is to get a region, called a monolayer, where the cells are spaced far enough apart to be counted and differentiated. The monolayer is found in the "feathered edge" created by the spreader slide as it draws the blood forward.
352:, are also visible on the blood smear. Modern complete blood count analyzers can provide an automated white blood cell differential, but they have a limited ability to differentiate immature and abnormal cells, so manual examination of the blood smear is frequently indicated. 415:
The biggest pitfall in most laboratories in developed countries is leaving too great a delay between taking the blood sample and making the blood smears. As blood cools to room temperature, male gametocytes will divide and release
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The preferred and most reliable diagnosis of malaria is microscopic examination of blood smears, because each of the four major parasite species has distinguishing characteristics. Two sorts of blood smear are traditionally used.
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J. Gerard; E. Lebas; A. Godon; O. Blanchet; F. Genevieve; A. Mercat; M. Zandecki (2007). "Free and intracellular bacteria on peripheral blood smears: an uncommon situation related to an adverse prognosis".
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is left for several hours in EDTA, the buildup of acid in the sample will cause the parasitised erythrocytes to shrink and the parasite will roll up, simulating the appearance of
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After staining, the monolayer is viewed under a microscope using magnification up to 1000 times. Individual cells are examined and their morphology is characterized and recorded.
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Two push-type peripheral blood smears suitable for characterization of cellular blood elements. Left smear is unstained, right smear is stained with Wright-Giemsa stain.
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and then stained in such a way as to allow the various blood cells to be examined microscopically. Blood smears are examined in the investigation of
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are similar to usual blood films and allow species identification, because the parasite's appearance is best preserved in this preparation.
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The left image shows a microscopic view of a normal adult blood film, while the right image shows a blood film from a patient with
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Microscopic examination of the shape, size, and coloration of red blood cells is useful for determining the cause of
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Blood smear examination is the preferred diagnostic method for certain parasitic infections, such as malaria and
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will rupture and merozoites invading erythrocytes will mistakenly give the appearance of the accolé form of
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John P. Greer; Sherrie L. Perkins (December 2008). "Chapter 1: Examination of the blood and bone marrow".
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Closeups of the feathered edge of blood smears. The pale middle band of the gradient is the monolayer.
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Linné & Ringsrud's Clinical Laboratory Science: Concepts, Procedures, and Clinical Applications
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Mary Louise Turgeon (23 March 2015). "Chapter 11: Principles and practice of clinical hematology".
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Routine analysis of blood in medical laboratories is usually performed on blood films stained with
686:. Vol. 1 (12th ed.). Philadelphia, PA: Lippincott Williams & Wilkins. pp. 5–9. 1765: 1408: 1403: 1062: 469: 310: 295: 197: 1737: 1510: 1229: 1224: 836: 758: 730: 687: 662: 595: 577: 536: 506: 349: 325: 208: 65: 1636: 1533: 1528: 1471: 1454: 1155: 1077: 826: 818: 720: 652: 585: 569: 558:"Purpose and Criteria for Blood Smear Scan, Blood Smear Examination, and Blood Smear Review" 235:
often use other specialized stains to aid in the differential diagnosis of blood disorders.
220: 204: 118: 420:: these are long sinuous filamentous structures that can be mistaken for organisms such as 1421: 614: 251: 232: 1219: 1732: 1641: 1570: 1391: 1340: 1182: 962: 921: 590: 557: 345: 284: 224: 831: 806: 1754: 1560: 1543: 1487: 1440: 1067: 992: 445: 145:
A blood smear is made by placing a drop of blood on one end of a slide, and using a
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Hempelmann E, Wilson RJ (1982). "Immunoprecipitation of malarial enzymes".
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Blood smears showing various developmental stages of the malaria parasite
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Gulati, Gene; Song, Jinming; Dulau Florea, Alina; Gong, Jerald (2013).
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in order to investigate abnormal results or confirm results that the
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Blood smear examination is usually performed in conjunction with a
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can be determined from the blood smear. This is known as a manual
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Variations of red blood cell shape on blood film, overall termed
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are used. The anticoagulant that causes the least problems is
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This article is about the microscope slide. For films named
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Denise Harmening (2009). "Chapter 31: Hematology methods".
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result in characteristic abnormalities on the blood film.
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6.8): malaria blood films must be stained at pH 7.2, or
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identification is always based on several trophozoites.
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capture procedures (rapid diagnostic tests such as the
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The slide is left to air dry, after which the blood is
780:"CDC - DPDx - Diagnostic Procedures - Blood Specimens" 1710: 1519: 1419: 1321: 1258: 1193: 1163: 1154: 1124: 1076: 1050: 955: 929: 920: 74: 64: 47: 535:(7th ed.). Elsevier Mosby. pp. 321–323. 502:Clinical Hematology and Fundamentals of Hemostasis 641:"Automated blood cell counts: state of the art" 424:. If the blood is kept at warmer temperatures, 35:. For films with excessive blood and gore, see 1695:aPTT-based activated protein C resistance test 1700:ETP-based activated protein C resistance test 1296: 897: 524: 522: 8: 613:Choladda Vejabhuti Curry (14 January 2015). 1303: 1289: 1281: 1160: 926: 904: 890: 882: 200:to distinguish the cells from each other. 53: 1356:Mean corpuscular hemoglobin concentration 830: 724: 656: 589: 192:to the slide by immersing it briefly in 1685:Ristocetin-induced platelet aggregation 1647:Activated protein C–protein C inhibitor 491: 774: 772: 645:American Journal of Clinical Pathology 639:Buttarello, M; Plebani, M (Jul 2008). 324:The proportions of different types of 44: 505:(5th ed.). F. A. Davis Company. 7: 1728:Nitro blue tetrazolium chloride test 1690:Activated protein C resistance test 407:species can be much more difficult. 87: 33:Blood (disambiguation) § Films 25: 1581:Dilute Russell's viper venom time 1366:Red blood cell distribution width 807:"Laboratory diagnosis of malaria" 805:Warhurst DC, Williams JE (1996). 319:microangiopathic hemolytic anemia 42:Stained blood on microscope slide 1593:Calibrated automated thrombogram 444:. This problem is made worse if 259: 250: 169: 157: 480:malaria antigen detection tests 1622:Coagulation activation markers 1495:Erythrocyte sedimentation rate 683:Wintrobe's Clinical Hematology 1: 1632:Thrombin–antithrombin complex 1380:White blood cell differential 562:Annals of Laboratory Medicine 330:white blood cell differential 1617:Overall hemostatic potential 751:Annales de biologie clinique 713:Clinical Infectious Diseases 1539:Partial thromboplastin time 1505:Monocyte distribution width 1351:Mean corpuscular hemoglobin 298:has flagged as unreliable. 1782: 1478:Hemoglobin electrophoresis 707:Jon E. Rosenblatt (2009). 615:"Differential Blood Count" 26: 1627:Prothrombin fragments 1+2 1588:Thrombin generation assay 1385:Absolute neutrophil count 1203:Hypersegmented neutrophil 1110:Hemoglobin precipitation 1040:Hereditary stomatocytosis 986:Hereditary elliptocytosis 88: 52: 1235:Critical green inclusion 1026:Sickle cell/drepanocyte 998:Hereditary spherocytosis 658:10.1309/EK3C7CTDKNVPXVTN 271:chronic myeloid leukemia 1549:Activated clotting time 1361:Mean corpuscular volume 967:Membrane abnormalities 574:10.3343/alm.2013.33.1.1 472:dots will not be seen. 1483:Sickle solubility test 1375:White blood cell count 1346:Red blood cell indices 1137:Red cell agglutination 877:Blood photomicrographs 389: 307:iron deficiency anemia 287: 107:peripheral blood smear 1680:Von Willebrand factor 1659:Euglobulin lysis time 1598:ST Genesia-based test 476:Immunochromatographic 378:Plasmodium falciparum 374: 282: 242:Clinical significance 176:Wright-Giemsa stained 1673:-antiplasmin complex 1610:Thrombodynamics test 1576:Ecarin clotting time 1465:Kleihauer–Betke test 1397:Mean platelet volume 1341:Red blood cell count 1323:Complete blood count 1240:Alder–Reilly anomaly 1094:Basophilic stippling 823:10.1136/jcp.49.7.533 315:megaloblastic anemia 305:. Disorders such as 292:complete blood count 1605:Thromboelastography 1215:Pelger–HuĂ«t anomaly 1173:Reactive lymphocyte 1099:Pappenheimer bodies 1030:Sickle cell disease 129:, such as those of 117:smeared on a glass 113:is a thin layer of 1435:Total blood volume 1409:Reticulocyte index 1404:Reticulocyte count 1063:Hypochromic anemia 390: 311:sickle cell anemia 296:automated analyzer 288: 233:Hematopathologists 1748: 1747: 1738:Immunophenotyping 1511:Osmotic fragility 1278: 1277: 1274: 1273: 1230:Toxic vacuolation 1225:Toxic granulation 1156:White blood cells 1150: 1149: 1089:Howell–Jolly body 786:. 4 November 2020 693:978-0-7817-6507-7 542:978-0-323-22545-8 512:978-0-8036-1732-2 350:toxic granulation 326:white blood cells 205:Romanowsky stains 99: 98: 16:(Redirected from 1773: 1637:Fibrinopeptide A 1534:Prothrombin time 1529:Clotting factors 1472:Hemoglobinopathy 1455:Fetal hemoglobin 1305: 1298: 1291: 1282: 1245:Jordans' anomaly 1161: 1078:Inclusion bodies 927: 906: 899: 892: 883: 864: 863: 851: 845: 844: 834: 802: 796: 795: 793: 791: 776: 767: 766: 745: 739: 738: 728: 719:(7): 1103–1108. 704: 698: 697: 677: 671: 670: 660: 636: 630: 629: 627: 625: 610: 604: 603: 593: 553: 547: 546: 526: 517: 516: 496: 466:SchĂĽffner's dots 263: 254: 221:white blood cell 173: 161: 119:microscope slide 92:edit on Wikidata 84: 57: 45: 31:or similar, see 21: 1781: 1780: 1776: 1775: 1774: 1772: 1771: 1770: 1751: 1750: 1749: 1744: 1723:Blood viscosity 1706: 1672: 1642:Fibrin monomers 1515: 1460:Apt–Downey test 1447:Red cell volume 1422:red blood cells 1420:Other tests of 1415: 1317: 1309: 1279: 1270: 1254: 1189: 1146: 1120: 1072: 1046: 951: 922:Red blood cells 916: 910: 873: 868: 867: 853: 852: 848: 804: 803: 799: 789: 787: 778: 777: 770: 747: 746: 742: 706: 705: 701: 694: 679: 678: 674: 638: 637: 633: 623: 621: 612: 611: 607: 555: 554: 550: 543: 528: 527: 520: 513: 498: 497: 493: 488: 381:, stained with 369: 277: 276: 275: 274: 266: 265: 264: 256: 255: 244: 231:abnormalities. 186: 185: 184: 183: 179: 178: 177: 174: 166: 165: 162: 143: 95: 80: 60: 43: 40: 23: 22: 15: 12: 11: 5: 1779: 1777: 1769: 1768: 1763: 1753: 1752: 1746: 1745: 1743: 1742: 1741: 1740: 1733:Flow cytometry 1730: 1725: 1720: 1714: 1712: 1708: 1707: 1705: 1704: 1703: 1702: 1697: 1687: 1682: 1677: 1676: 1675: 1670: 1666: 1661: 1651: 1650: 1649: 1644: 1639: 1634: 1629: 1619: 1614: 1613: 1612: 1602: 1601: 1600: 1595: 1585: 1584: 1583: 1578: 1573: 1571:Reptilase time 1563: 1558: 1557: 1556: 1551: 1546: 1541: 1536: 1525: 1523: 1517: 1516: 1514: 1513: 1508: 1502: 1497: 1492: 1491: 1490: 1485: 1480: 1469: 1468: 1467: 1462: 1452: 1451: 1450: 1444: 1438: 1426: 1424: 1417: 1416: 1414: 1413: 1412: 1411: 1401: 1400: 1399: 1392:Platelet count 1389: 1388: 1387: 1382: 1372: 1371: 1370: 1369: 1368: 1363: 1358: 1353: 1338: 1333: 1327: 1325: 1319: 1318: 1310: 1308: 1307: 1300: 1293: 1285: 1276: 1275: 1272: 1271: 1269: 1268: 1262: 1260: 1256: 1255: 1253: 1252: 1247: 1242: 1237: 1232: 1227: 1222: 1217: 1212: 1211: 1210: 1199: 1197: 1191: 1190: 1188: 1187: 1186: 1185: 1183:Russell bodies 1175: 1169: 1167: 1158: 1152: 1151: 1148: 1147: 1145: 1144: 1139: 1134: 1128: 1126: 1122: 1121: 1119: 1118: 1117: 1116: 1108: 1107: 1106: 1101: 1096: 1091: 1085:Developmental 1082: 1080: 1074: 1073: 1071: 1070: 1065: 1060: 1054: 1052: 1048: 1047: 1045: 1044: 1043: 1042: 1034: 1033: 1032: 1024: 1019: 1014: 1009: 1004: 1003: 1002: 1001: 1000: 990: 989: 988: 978: 973: 965: 963:Poikilocytosis 959: 957: 953: 952: 950: 949: 944: 939: 933: 931: 924: 918: 917: 911: 909: 908: 901: 894: 886: 880: 879: 872: 871:External links 869: 866: 865: 846: 797: 768: 740: 726:10.1086/605574 699: 692: 672: 631: 605: 548: 541: 518: 511: 490: 489: 487: 484: 446:anticoagulants 409: 408: 401: 368: 365: 346:acute leukemia 285:poikilocytosis 268: 267: 258: 257: 249: 248: 247: 246: 245: 243: 240: 225:red blood cell 209:Wright's stain 181: 180: 175: 168: 167: 163: 156: 155: 154: 153: 152: 147:spreader slide 142: 139: 97: 96: 89: 86: 85: 78: 72: 71: 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1393: 1390: 1386: 1383: 1381: 1378: 1377: 1376: 1373: 1367: 1364: 1362: 1359: 1357: 1354: 1352: 1349: 1348: 1347: 1344: 1343: 1342: 1339: 1337: 1334: 1332: 1329: 1328: 1326: 1324: 1320: 1316: 1313: 1306: 1301: 1299: 1294: 1292: 1287: 1286: 1283: 1267: 1264: 1263: 1261: 1257: 1251: 1248: 1246: 1243: 1241: 1238: 1236: 1233: 1231: 1228: 1226: 1223: 1221: 1218: 1216: 1213: 1209: 1206: 1205: 1204: 1201: 1200: 1198: 1196: 1192: 1184: 1181: 1180: 1179: 1176: 1174: 1171: 1170: 1168: 1166: 1162: 1159: 1157: 1153: 1143: 1140: 1138: 1135: 1133: 1130: 1129: 1127: 1123: 1115: 1112: 1111: 1109: 1105: 1102: 1100: 1097: 1095: 1092: 1090: 1087: 1086: 1084: 1083: 1081: 1079: 1075: 1069: 1068:Polychromasia 1066: 1064: 1061: 1059: 1056: 1055: 1053: 1049: 1041: 1038: 1037: 1035: 1031: 1028: 1027: 1025: 1023: 1020: 1018: 1015: 1013: 1010: 1008: 1005: 999: 996: 995: 994: 991: 987: 984: 983: 982: 979: 977: 974: 972: 969: 968: 966: 964: 961: 960: 958: 954: 948: 945: 943: 940: 938: 935: 934: 932: 928: 925: 923: 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Retrieved 618: 608: 565: 561: 551: 531: 501: 494: 474: 441: 437: 433: 429: 421: 418:microgametes 414: 410: 404:Thick smears 403: 397: 391: 387:Giemsa stain 383:Wright stain 376: 354: 338:eosinophilia 334:neutrophilia 323: 300: 289: 237: 213:Giemsa stain 202: 187: 146: 144: 110: 106: 102: 100: 37:slasher film 28: 1761:Blood tests 1521:Coagulation 1500:Haptoglobin 1315:blood tests 1178:Smudge cell 1165:Lymphocytes 1104:Cabot rings 1017:Schistocyte 981:Elliptocyte 971:Acanthocyte 790:20 February 784:www.cdc.gov 442:P. malariae 398:Thin smears 342:blast cells 141:Preparation 103:blood smear 76:MedlinePlus 48:Blood smear 1755:Categories 1718:Blood film 1554:Fibrinogen 1336:Hematocrit 1331:Hemoglobin 1312:Hematology 1250:Left shift 1132:Haemoconia 1114:Heinz body 1012:Echinocyte 993:Spherocyte 913:Blood film 568:(1): 1–7. 486:References 357:babesiosis 135:filariasis 111:blood film 18:Blood film 1766:Pathology 1669:Plasmin-α 1022:Degmacyte 1007:Dacrocyte 582:2234-3806 426:schizonts 217:Diff-Quik 164:Unstained 127:parasites 1474:testing 1266:Auer rod 1142:Rouleaux 976:Codocyte 915:findings 763:17264045 735:19691431 667:18550479 619:Medscape 600:23301216 448:such as 438:P. ovale 434:P. vivax 422:Borrelia 344:seen in 229:platelet 207:such as 194:methanol 66:ICD-9-CM 1664:D-Dimer 841:8813948 624:12 June 591:3535191 454:citrate 450:heparin 367:Malaria 198:stained 131:malaria 1051:Colour 862:: 637. 839:  832:500564 829:  761:  733:  690:  665:  598:  588:  580:  539:  509:  470:James' 361:sepsis 303:anemia 227:, and 82:003665 1711:Other 1507:(MDW) 1449:(RCV) 1437:(TBV) 1259:Other 1125:Other 956:Shape 432:. If 215:, or 190:fixed 115:blood 90:[ 29:Blood 1443:(PV) 930:Size 837:PMID 792:2022 759:PMID 731:PMID 688:ISBN 663:PMID 626:2019 596:PMID 578:ISSN 537:ISBN 507:ISBN 468:and 458:EDTA 385:and 336:and 317:and 133:and 70:90.5 827:PMC 819:doi 721:doi 653:doi 649:130 586:PMC 570:doi 452:or 436:or 109:or 1757:: 860:29 858:. 835:. 825:. 815:49 813:. 809:. 782:. 771:^ 755:65 753:. 729:. 717:49 715:. 711:. 661:. 647:. 643:. 617:. 594:. 584:. 576:. 566:33 564:. 560:. 521:^ 462:pH 363:. 313:, 309:, 223:, 211:, 137:. 105:, 101:A 1671:2 1304:e 1297:t 1290:v 905:e 898:t 891:v 843:. 821:: 794:. 765:. 737:. 723:: 696:. 669:. 655:: 628:. 602:. 572:: 545:. 515:. 273:. 94:] 39:. 20:)

Index

Blood film
Blood (disambiguation) § Films
slasher film

ICD-9-CM
MedlinePlus
003665
edit on Wikidata
blood
microscope slide
hematological
parasites
malaria
filariasis
Unstained smear
Stained smear
fixed
methanol
stained
Romanowsky stains
Wright's stain
Giemsa stain
Diff-Quik
white blood cell
red blood cell
platelet
Hematopathologists
Normal human blood film
Blood film in chronic myeloid leukemia
chronic myeloid leukemia

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