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In 1973, she was hired as an assistant professor at
University of California, San Francisco (UCSF). After a tough pre-tenure review in 1976, she found support in a group of women and men who met informally for 20 years to help each other thrive in academia. She was a professor of biochemistry and
262:
Guthrie showed that yeast have introns in their pre-messenger RNAs. They also have small nuclear RNAs (snRNAs) involved in splicing pre-messenger RNA into messenger RNA in eukaryotic cells. 2 In work described in her citation for the
Genetics Society of America Medal as a “macromolecular tour de
1122:
271:. Her work established the role of base pairing between the snRNAs and their target sequences at each step in the removal of an intron and allowed identification of proteins that formed part of the spliceosome complex with the snRNAs.
233:
She was the daughter of
Brooklyn native and humorist Irene Kampen, whose book, Life Without George, was the basis for The Lucy Show, which aired for six seasons on CBS in the 1960s. (Lucy's daughter on the show was named Chris.)
1127:
675:
Vijayraghavan U., Parker R., Tamm J., Iimura Y., Rossi J., et al., 1986 “Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome.”
267:
genes for the yeast snRNAs. To accomplish this feat, she had to invent methods to discriminate functional snRNAs from degradation products and also to create widely used intron-containing
1137:
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319:
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Riedel N., Wise J. A., Swerdlow H., Mak A., Guthrie C., 1986 “Small nuclear RNAs from
Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity.”
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Siliciano P. G., Brow D. A., Roiha H., Guthrie C., 1987a “An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA.”
534:
Madhani H. D., Guthrie C., 1992 “A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome.”
564:
Parker R., Guthrie C., 1985 “A point mutation in the conserved hexanucleotide at a yeast 5′ splice junction uncouples recognition, cleavage, and ligation.”
764:
Jonathan
Weisman, Christine Guthrie, and Gerald Fink editors, 2010. Guide to Yeast Genetics: Functional Genomics, Proteomics and other Systems Analysis.
574:
Parker R., Siliciano P. G., Guthrie C., 1987 “Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA.”
1132:
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Jandrositz A., Guthrie C., 1995 “Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs.”
654:
Siliciano P. G., Guthrie C., 1988 “5′ splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.”
484:
Guthrie C., Nashimoto H., Nomura M., 1969 “Structure and function of E. coli ribosomes. 8. Cold-sensitive mutants defective in ribosome assembly.”
422:
A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates.
192:
the genes for yeast snRNA and established the role of base pairing between the snRNAs and their target sequences at each step in the removal of an
165:
133:
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Burgess S., Couto J. R., Guthrie C., 1990 “A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing.”
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687:
Wise J. A., Tollervey D., Maloney D., Swerdlow H., Dunn E. J., et al., 1983 “Yeast contains small nuclear RNAs encoded by single copy genes.”
221:
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Noble S. M., Guthrie C., 1996 “Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations.”
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Siliciano P. G., Jones M. H., Guthrie C., 1987b “Saccharomyces cerevisiae has a U1-like small nuclear RNA with unexpected properties.”
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Shannon K. W., Guthrie C., 1991 “Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.”
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Lesser C. F., Guthrie C. 1993 “Mutations in U6 snRNA that alter splice site specificity: Implications for the active site.”
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Schwer B., Guthrie C., 1992 “A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis.” E
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Strauss E. J., Guthrie C., 1991 “A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.”
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Patterson B., Guthrie C., 1987 “An essential yeast snRNA with a U5-like domain is required for splicing in vivo.”
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Schwer B., Guthrie C., 1991 “PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome.”
75:
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217:
65:
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Madhani H. D., Bordonne R., Guthrie C. 1990 “Multiple roles for U6 snRNA in the splicing pathway.”
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1987 “A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.”
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Brow D. A., Guthrie C., 1988 “Spliceosomal RNA U6 is remarkably conserved from yeast to mammals.”
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may contain an excessive amount of intricate detail that may interest only a particular audience
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1986 “Activation of a cryptic TACTAAC box in the
Saccharomyces cerevisiae actin intron.”
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Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein.”
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268:
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Christine
Guthrie was born in Brooklyn, New York. She received a BS in Zoology from
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1002:
807:
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Madhani H. D., Guthrie C., 1994 “Dynamic RNA-RNA interactions in the spliceosome.”
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Christine
Guthrie (1945–2022). RNA trailblazer who illuminated splicing mechanics.
1123:
University of
Wisconsin–Madison College of Agricultural and Life Sciences alumni
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969:"Nature Structural & Molecular Biology "Editorial: Telling it like it was""
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Every Other
Thursday: Stories and Strategies from Successful Women Scientists
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any relevant information, and removing excessive detail that may be against
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American Cancer Society Research Professor of Genetics at UCSF.
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and American Cancer Society Research Professor of Genetics at
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Suzanne Noble, Sean M. Burgess, and Evelyn Strauss (2022):
1128:
Members of the United States National Academy of Sciences
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American Society for Biochemistry and Molecular Biology
893:
American Society for Biochemistry and Molecular Biology
438:
Cellini A., Parker R., McMahon J., Guthrie C., Rossi J.
196:. She also identified proteins that formed part of the
864:
Guide to Yeast Genetics and Molecular and Cell Biology
721:
Guide to Yeast Genetics and Molecular and Cell Biology
706:
Guide to Yeast Genetics and Molecular and Cell Biology
494:
Guthrie C., Patterson B., 1988 “Spliceosomal snRNAs.”
749:
Christine Guthrie and Gerald R. Fink, editors. 2002
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Christine Guthrie and Gerald R. Fink, editors. 2002
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Christine Guthrie and Gerald R. Fink, editors. 2002
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751:Guide to Yeast Genetics and Molecular Cell Biology
736:Guide to Yeast Genetics and Molecular Cell Biology
208:, an influential methods series for many years.
1138:Fellows of the American Academy of Microbiology
1113:University of California, San Francisco faculty
315:2006 The RNA Society Lifetime Achievement Award
1037:"John Abelson to receive WSU top alumni award"
206:Guide to Yeast Genetics and Molecular Biology
8:
808:"Genetics Society Award: Christine Guthrie"
452:Couto J. R., Tamm J., Parker R., Guthrie C.
308:1998 WICB Senior Career Recognition Award (
18:
984:
973:Nature Structural & Molecular Biology
382:Learn how and when to remove this message
916:Science. Vol 377, Issue 6610, p. 1049,
839:"NAS Member Directory:Christine Guthrie"
200:complex with the snRNAs. Elected to the
781:
250:Scanning Electron Micrograph of Yeast,
134:University of California, San Francisco
1019:Yale University Press, New Haven, CT.
166:University of California San Francisco
263:force”, she cloned and sequenced the
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889:"With a little help from my friends"
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160:(1945-2022) was an American yeast
14:
1067:American Society for Cell Biology
862:Christine Guthrie, editor (2002)
310:American Society for Cell Biology
304:Genetics Society of America Medal
107:Genetics Society of America Medal
1133:Scientists from New York (state)
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704:Christine Guthrie, editor 2002
979:(8): 663–664. August 1, 2006.
1:
1118:University of Michigan alumni
168:. She showed that yeast have
843:National Academy of Sciences
364:Knowledge's inclusion policy
298:National Academy of Sciences
202:National Academy of Sciences
1041:Washington State University
918:doi:10.1126/science.ade2163
815:Genetics Society of America
772:2nd edition Academic Press.
220:and a PhD in genetics from
1159:
1093:American women geneticists
486:Proc. Natl. Acad. Sci. USA
331:Selected scientific papers
596:Proc Natl. Acad. Sci. USA
418:Burgess S. M., Guthrie C.
204:in 1993, Guthrie edited
151:
112:
1108:Scientists from Brooklyn
252:Saccharomyces cerevisiae
222:University of Wisconsin.
212:Early life and education
279:Guthrie was married to
176:pre-messenger RNA into
76:University of Wisconsin
254:
218:University of Michigan
66:University of Michigan
868:Methods in Enzymology
766:Methods in Enzymology
755:Methods in Enzymology
740:Methods in Enzymology
725:Methods in Enzymology
710:Methods in Enzymology
249:
172:(snRNAs) involved in
1103:American geneticists
986:10.1038/nsmb0806-663
887:Guthrie, Christine.
506:Euro Mol Biol Org J.
224:Her PhD advisor was
1015:Ellen Daniell 2008
936:"ASBMB-Merck Award"
16:American geneticist
946:on October 2, 2016
616:uro Mol Bio Org J.
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170:small nuclear RNAs
43:Brooklyn, New York
1043:. October 5, 2004
678:uro Mol Bio Org J
546:Annu. Rev. Genet.
496:Annu. Rev. Genet.
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320:ASBMB-Merck Award
158:Christine Guthrie
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114:Scientific career
23:Christine Guthrie
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184:cells. Guthrie
141:Doctoral advisor
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444:Mol. Cell Biol.
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354:Please help by
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238:Academic career
226:Masayasu Nomura
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146:Masayasu Nomura
72:Alma mater
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1070:. Retrieved
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976:
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948:. Retrieved
944:the original
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896:. Retrieved
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728:
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691:
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684:: 1683–1695.
681:
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635:
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621:: 5033–5039.
618:
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601:: 8097–8101.
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531:: 2264–2277.
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521:: 1982–1988.
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157:
156:
130:Institutions
113:
97:John Abelson
85:Genetics of
55:(2022-07-01)
53:July 1, 2022
1143:1945 births
1072:October 12,
1047:October 14,
950:October 12,
898:October 12,
848:October 12,
820:October 12,
198:spliceosome
1087:Categories
866:, Part B,
777:References
708:, Part B,
694:: 743–751.
667:Genes Dev.
662:1258–1267.
651:1484–1487.
641:: 585–592.
631:: 773–785.
626:Genes Dev.
611:: 494–499.
591:: 613–624.
581:: 229–239.
571:: 107–118.
541:: 803–817.
511:: 820–832.
491:: 384–391.
481:: 157–163.
466:Guthrie C.
463:: 445–455.
458:Genes Dev.
449:1571–1578.
415:: 705–717.
372:April 2020
360:relocating
285:biochemist
182:eukaryotic
162:geneticist
35:1945-04-27
723:, Part A
656:Genes Dev
526:Genes Dev
432:1377–1391
190:sequenced
62:Education
1003:21258733
995:16886003
672:629–641.
561:: 67–80.
556:Genetics
501:387–419.
420:, 1993 “
405:213–218.
258:Research
174:splicing
124:Genetics
870:Volume
768:volume
757:volume
753:Part C
742:volume
738:Part B
727:volume
712:Volume
646:Science
551:: 1–26.
516:Science
476:Science
1023:
1001:
993:
606:Nature
424:” Cell
397:Nature
291:Awards
194:intron
186:cloned
120:Fields
103:Awards
93:Spouse
999:S2CID
811:(PDF)
699:Books
470:1991
326:Works
318:2011
302:1997
296:1993
87:yeast
1074:2018
1049:2018
1021:ISBN
991:PMID
952:2018
900:2018
850:2018
822:2018
689:Cell
649:237:
636:Cell
586:Cell
576:Cell
566:Cell
536:Cell
410:Cell
188:and
50:Died
29:Born
981:doi
872:350
770:470
759:351
744:351
729:351
714:350
609:349
559:143
529:. 4
519:262
499:22:
479:253
400:334
358:or
265:SNR
180:in
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312:)
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Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
