Knowledge (XXG)

Exposing Microorganisms in the Stratosphere

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Of the 40 million spores exposed to the stratosphere, only 267 spores (or 0.0007%) remained viable. Extrapolating this, no viable spores would remain if flight samples had an additional 150  minutes of Sun exposure in the stratosphere (630 min total time). The survivors showed three single nucleotide
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The 80-pound gondola features four doors that rotate to expose up to 10 experimental samples each for 8 hours. To terminate the flight, an explosive charge detonated, tearing a hole in the balloon. E-MIST and other science payloads attached to the balloon's gondola were returned to the surface under
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Even after cleaning, spacecraft leaving Earth still carry microorganisms on board that are embedded within surfaces, instruments, electronics, and other inaccessible areas that cannot be readily cleaned and are not exposed to direct sunlight, so there is a concern about the few survivors, which may
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After 8 hours exposure in the stratosphere (31 km above sea level), 99.9% of the entire population was destroyed. According to the researchers, "most terrestrial bacteria would be inactivated within the first on Mars if contaminated spacecraft surfaces receive direct sunlight."
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Beyond investigating viability alone, concerns of whether microorganisms can survive deep space conditions and potentially be transported to other planets, such as Mars, has long been a concern to scientists. To further this understanding, it is necessary to determine if there is a
171:(base pair) substitutions compared to unexposed controls kept on the ground. These three coding regions are associated with bacterial sporulation and metabolism. A similar observation was recorded on SAFR-032 samples that were exposed outside the International Space Station. 158:
a parachute, where waiting researchers recovered the biological samples for analysis. Major payload components were a lithium-ion battery, an ultraviolet (UV) radiometer, humidity and temperatures sensors, and a flight computer.
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be pushed in evolutionarily consequential directions. The researchers suggest that ultraviolet-C lamps (UVC LED arrays) could be embedded in lander spacecraft hardware to help sterilize their interior.
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A test flight of the balloon and gondola was launched from New Mexico on 24 August 2014. A second, longer flight, took place on 10 October 2015.
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could provide valuable insight into the functional effects on surviving microorganisms. For instance, this approach could help identify
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Smith, David J. (21 October 2013). "Microbes in the Upper Atmosphere and Unique Opportunities for Astrobiology Research".
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The environmental conditions in the upper atmosphere resemble the surface conditions of Mars, especially in the
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Stratosphere Conditions Inactivate Bacterial Endospores from a Mars Spacecraft Assembly Facility
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of every major biological lineage have been detected in Earth's upper atmosphere. If microbial
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Smith, David J.; Thakrar, Prital J.; Bharrat, Anthony E.; Dokos, Adam G.; et al.
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Stowaway Bacteria Could Be Even More Contagious in Space Than We Thought
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is achievable, independent of Earth's surface, then the search for
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Figliozzi, Gianine M.; Walter, Daryl (25 September 2015).
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bacteria and their spores by helium-filled balloon to the
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Astrobiology Science and Technology for Exploring Planets
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Exposing Microorganisms in the Stratosphere (E-MIST)
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Exposing Microorganisms in the Stratosphere (E-MIST)
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(25 September 2015). 22:Exposing Microorganisms in the Stratosphere 2710: 2484: 2414: 2400: 2392: 2137:Enceladus Life Signatures and Habitability 1841: 1568: 1554: 1546: 1308: 1089: 588: 558: 544: 536: 2324:European Astrobiology Network Association 1817:List of potentially habitable exoplanets 2998: 2235:Living Interplanetary Flight Experiment 259: 2225:Biological Oxidant and Life Detection 7: 2976: 2374: 1802:Habitability of yellow dwarf systems 1787:Habitability of neutron star systems 432:Astrobiology Science Conference 2015 1777:Habitability of binary star systems 449:NASA's John F. Kennedy Space Center 2339:Nexus for Exoplanet System Science 1782:Habitability of natural satellites 1707:Hypothetical types of biochemistry 206:E-MIST study - scientific balloon. 194:E-MIST study - preparing hardware. 14: 2319:Center for Life Detection Science 2240:Mars Astrobiology Explorer-Cacher 1792:Habitability of red dwarf systems 32:study to determine if a specific 3025: 3013: 3001: 2975: 2966: 2965: 2373: 2362: 2361: 2344:Ocean Worlds Exploration Program 2177:Life Investigation For Enceladus 1697:Extraterrestrial sample curation 1528: 1517: 1516: 199: 187: 2664:Bacterial cellular morphologies 2282:Astrobiology Society of Britain 2157:Explorer of Enceladus and Titan 1812:Habitable zone for complex life 2167:Journey to Enceladus and Titan 312:. April 2017, 17(4): 337-350. 1: 2215:Astrobiology Field Laboratory 1767:Extraterrestrial liquid water 391:Gravitational Spaqce Research 2349:Spanish Astrobiology Center‎ 1757:Circumstellar habitable zone 973:Recurring slope lineae (RSL) 226:Interplanetary contamination 3048:Astrobiology space missions 2334:NASA Astrobiology Institute 1407:Inspiration Mars Foundation 3084: 2907:Bacteria (classifications) 2629:Primary nutritional groups 2182:Mars sample return mission 1442:Artificial objects on Mars 135:associated with repairing 3068:Microorganisms and humans 2961: 2357: 2260:Terrestrial Planet Finder 1682:Earliest known life forms 1511: 1459:List of films set on Mars 1274:C/2013 A1 (Siding Spring) 1071:Classical albedo features 573: 3053:Biological contamination 2783:Bacterial outer membrane 2292:Breakthrough Initiatives 1377:List of missions to Mars 40:. The study transported 1953:Mars Science Laboratory 1772:Galactic habitable zone 1535:Solar System portal 2778:Gram-negative bacteria 2757:Gram-positive bacteria 2132:Enceladus Life Finder‎ 2112:Breakthrough Enceladus 1636:Planetary oceanography 1149:Solar eclipses on Mars 1008:"Swiss cheese" feature 864:Concentric crater fill 3058:Extraterrestrial life 2633:Substrate preference 2307:Breakthrough Starshot 1907:Lunar Micro Ecosystem 1827:Superhabitable planet 1692:Extraterrestrial life 526:Tersicoccus phoenicis 356:10.1089/ast.2013.1074 318:10.1089/ast.2016.1549 269:"Hitchhiking to Mars" 247:Tersicoccus phoenicis 16:NASA research program 2614:Microbial metabolism 2314:Carl Sagan Institute 2302:Breakthrough Message 2142:Enceladus Orbilander 1877:Biosatellite program 1727:Planetary protection 1611:Evolutionary biology 1601:Atmospheric sciences 1349:Permanent settlement 241:Planetary protection 94:planetary protection 2864:Non-motile bacteria 2460:Pathogenic bacteria 2297:Breakthrough Listen 1491:Timekeeping on Mars 1168:Planetary transits 1153:Satellite transits 1066:Observation history 914:Lobate debris apron 451:. 22 September 2014 348:2013AsBio..13..981S 2793:Lipopolysaccharide 2127:Enceladus Explorer 503:. Peter Dockrill, 137:damage to the cell 56:ionizing radiation 2989: 2988: 2888: 2887: 2834:Bacterial capsule 2800:Periplasmic space 2767:Lipoteichoic acid 2652: 2651: 2624:Microbial ecology 2619:Nitrogen fixation 2389: 2388: 2268: 2267: 2092:Rosalind Franklin 2005:Trace Gas Orbiter 1662:Allan Hills 84001 1641:Planetary science 1543: 1542: 1496:Sol (day on Mars) 1464:Martian scientist 1447:Memorials on Mars 1430: 1429: 1401:The Case for Mars 1298: 1297: 1079: 1078: 1013:Terrain softening 978:Ring mold craters 946:North Polar Basin 869:Dark slope streak 714:Vastitas Borealis 611:Dust devil tracks 117:solar irradiation 3075: 3030: 3029: 3028: 3018: 3017: 3006: 3005: 3004: 2997: 2979: 2978: 2969: 2968: 2917:Former groupings 2711: 2562:Human microbiome 2485: 2416: 2409: 2402: 2393: 2377: 2376: 2365: 2364: 1842: 1737:Yamato meteorite 1570: 1563: 1556: 1547: 1533: 1532: 1531: 1520: 1519: 1394:The Mars Project 1309: 1257: 1247: 1237: 1215: 1213: 1212: 1090: 951:Ocean hypothesis 801:Outflow 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1873: 1870: 1868: 1865: 1863: 1860: 1858: 1855: 1853: 1850: 1849: 1847: 1843: 1840: 1834: 1828: 1825: 1823: 1820: 1818: 1815: 1813: 1810: 1808: 1805: 1803: 1800: 1798: 1795: 1793: 1790: 1788: 1785: 1783: 1780: 1778: 1775: 1773: 1770: 1768: 1765: 1763: 1760: 1758: 1755: 1754: 1752: 1750: 1744: 1738: 1735: 1733: 1730: 1728: 1725: 1723: 1720: 1718: 1715: 1713: 1710: 1708: 1705: 1703: 1702:Extremophiles 1700: 1698: 1695: 1693: 1690: 1688: 1685: 1683: 1680: 1678: 1675: 1673: 1670: 1668: 1665: 1663: 1660: 1658: 1655: 1654: 1652: 1648: 1642: 1639: 1637: 1634: 1632: 1629: 1627: 1624: 1622: 1619: 1617: 1614: 1612: 1609: 1607: 1604: 1602: 1599: 1597: 1594: 1592: 1589: 1588: 1586: 1582: 1578: 1571: 1566: 1564: 1559: 1557: 1552: 1551: 1548: 1536: 1525: 1523: 1514: 1513: 1510: 1502: 1499: 1497: 1494: 1493: 1492: 1489: 1487: 1484: 1482: 1479: 1477: 1474: 1470: 1467: 1465: 1462: 1460: 1457: 1456: 1455: 1452: 1448: 1445: 1444: 1443: 1440: 1439: 1437: 1433: 1423: 1420: 1418: 1415: 1413: 1410: 1408: 1405: 1403: 1402: 1398: 1396: 1395: 1391: 1390: 1388: 1384: 1378: 1375: 1374: 1372: 1370: 1366: 1360: 1357: 1355: 1352: 1350: 1347: 1345: 1344:Human mission 1342: 1340: 1339:Sample return 1337: 1335: 1332: 1330: 1327: 1325: 1322: 1320: 1317: 1316: 1314: 1310: 1307: 1305: 1301: 1291: 1288: 1287: 1285: 1281: 1275: 1272: 1271: 1269: 1265: 1256: 1249: 1246: 1239: 1236: 1229: 1227: 1224: 1223: 1222: 1219: 1214: 1205: 1204: 1203: 1202:Mars-crossers 1200: 1198: 1195: 1194: 1192: 1190: 1186: 1178: 1175: 1173: 1170: 1169: 1167: 1163: 1160: 1158: 1155: 1154: 1152: 1150: 1147: 1146: 1144: 1142: 1138: 1130: 1127: 1125: 1122: 1121: 1120: 1117: 1113: 1110: 1108: 1105: 1104: 1103: 1100: 1099: 1097: 1095: 1091: 1088: 1086: 1082: 1072: 1069: 1067: 1064: 1062: 1059: 1057: 1054: 1052: 1049: 1048: 1046: 1044: 1040: 1034: 1031: 1029: 1026: 1024: 1021: 1019: 1018:Tharsis bulge 1016: 1014: 1011: 1009: 1006: 1004: 1001: 999: 996: 994: 991: 989: 986: 984: 981: 979: 976: 974: 971: 967: 964: 963: 962: 959: 957: 956:Ore resources 954: 952: 949: 947: 944: 942: 939: 935: 932: 930: 927: 926: 925: 922: 920: 917: 915: 912: 910: 907: 905: 902: 900: 897: 895: 892: 890: 887: 885: 882: 880: 877: 875: 872: 870: 867: 865: 862: 860: 857: 855: 852: 850: 849:Chaos terrain 847: 845: 842: 840: 839:Brain terrain 837: 836: 834: 832: 828: 822: 819: 817: 814: 812: 809: 807: 804: 802: 799: 797: 794: 790: 787: 786: 785: 782: 780: 777: 775: 772: 770: 767: 765: 762: 760: 757: 755: 754:Chaos terrain 752: 750: 747: 745: 742: 739: 735: 732: 731: 729: 727: 721: 715: 712: 710: 707: 705: 702: 700: 697: 695: 692: 690: 687: 685: 682: 680: 677: 675: 672: 670: 669:Planum Boreum 667: 665: 662: 660: 659:Olympia Undae 657: 655: 652: 650: 649:Eridania Lake 647: 645: 642: 640: 637: 635: 632: 631: 629: 627: 623: 617: 614: 612: 609: 607: 604: 602: 599: 598: 596: 594: 590: 587: 585: 581: 577: 572: 568: 561: 556: 554: 549: 547: 542: 541: 538: 532: 528: 527: 523: 521: 518: 517: 513: 506: 505:Science Alert 502: 497: 495: 493: 489: 477: 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1687:Earth analog 1672:Biosignature 1631:Paleontology 1626:Microbiology 1606:Biochemistry 1596:Astrophysics 1577:Astrobiology 1481:Life on Mars 1476:Flag of Mars 1417:Mars Society 1399: 1392: 1359:Terraforming 1354:Colonization 1124:Swift crater 966:polar wander 694:Terra Sabaea 634:Arabia Terra 525: 504: 481:29 September 479:. Retrieved 475: 465: 455:29 September 453:. Retrieved 448: 439: 431: 423: 411: 401:29 September 399:. Retrieved 394: 390: 339: 336:Astrobiology 335: 310:Astrobiology 309: 280:29 September 278:. Retrieved 272: 262: 245: 231:Life on Mars 221:Extremophile 173: 169: 165: 156: 144: 121: 101:stratosphere 98: 96:procedures. 68: 60: 48:stratosphere 41: 25: 21: 20: 18: 2839:Slime layer 2519:Facultative 2507:Facultative 2208:undeveloped 2058:BioSentinel 1948:Fobos-Grunt 1845:Earth orbit 1667:Biomolecule 1657:Abiogenesis 1650:Main topics 1584:Disciplines 1304:Exploration 1226:5261 Eureka 894:Groundwater 859:Composition 684:Tempe Terra 674:Quadrangles 601:Circulation 109:desiccation 90:atmospheres 82:replication 3042:Categories 2946:Mollicutes 2941:Firmicutes 2931:Prokaryota 2849:Glycocalyx 2674:plasticity 2637:Lipophilic 2490:preference 2465:Resistance 2255:Red Dragon 2035:OSIRIS-REx 2019:Comets and 1722:Panspermia 961:Polar caps 941:Mud cracks 924:Meteorites 909:Lava tubes 844:Carbonates 779:Labyrinthi 654:Iani Chaos 593:Atmosphere 255:References 236:Panspermia 139:envelope, 74:metabolism 3008:Astronomy 2899:evolution 2873:Composite 2772:Endospore 2730:Cell wall 2706:Structure 2597:Placental 2536:Nanaerobe 2514:Anaerobic 2445:Infection 2172:Laplace-P 2075:Dragonfly 2029:Hayabusa2 2021:asteroids 1990:Tianwen-1 1968:Mars 2020 1959:Curiosity 1892:Eu:CROPIS 1747:Planetary 1486:Sub-Earth 1469:Mythology 1422:Mars race 1189:Asteroids 1085:Astronomy 1056:Hesperian 1051:Amazonian 1023:Volcanism 998:Spherules 919:Marsquake 874:Dichotomy 789:by height 784:Mountains 584:Geography 434:. (2015). 113:oxidation 105:sea level 2971:Category 2894:Taxonomy 2827:envelope 2717:envelope 2607:Salivary 2524:Obligate 2502:Obligate 2450:Exotoxin 2427:Bacteria 2368:Category 2230:Kazachok 2220:Beagle 3 2152:ExoLance 2105:Proposed 1943:Beagle 2 1917:OREOcube 1897:EXOSTACK 1838:missions 1522:Category 1386:Advocacy 1369:Missions 1312:Concepts 1141:Transits 1112:Monolith 1061:Noachian 1033:Yardangs 929:on Earth 889:Glaciers 734:"Canals" 726:features 724:Physical 476:Phys Org 364:24106911 214:See also 65:Overview 3020:Biology 2994:Portals 2981:Commons 2880:Biofilm 2859:Fimbria 2844:S-layer 2825:Outside 2686:Bacilli 2602:Uterine 2587:Vaginal 2497:Aerobic 2480:ecology 2435:Medical 2380:Commons 2197:Trident 2187:Oceanus 2086:ExoMars 2067:Planned 2041:Rosetta 1996:Zhurong 1984:Phoenix 1922:Tanpopo 1912:O/OREOS 1857:BIOCORE 1454:Fiction 1435:Related 1329:Landing 1324:Orbiter 1283:General 1252:2007 NS 1242:1999 UJ 1232:1998 VF 1221:Trojans 1208:2007 WD 1197:Impacts 1177:Mercury 1043:History 1003:Surface 934:on Mars 899:Gullies 884:Geysers 831:Geology 821:Gravity 816:Valleys 769:Gullies 759:Craters 749:Catenae 744:Canyons 699:Tharsis 644:Cydonia 626:Regions 616:Methane 606:Climate 529:at the 344:Bibcode 179:Gallery 162:Results 153:Gondola 125:genomic 28:) is a 2926:Monera 2696:Spiral 2488:Oxygen 2329:MERMOZ 2122:CAESAR 2010:Viking 1927:VEGGIE 1902:EXPOSE 1882:E-MIST 1872:BIOPAN 1862:Biolab 1822:Tholin 1526:  1515:  1267:Comets 1162:Deimos 1157:Phobos 1119:Deimos 1102:Phobos 806:Plains 774:Mensae 764:Fossae 362:  141:genome 78:growth 26:E-MIST 2854:Pilus 2808:only: 2788:Porin 2780:only: 2759:only: 2681:Cocci 2657:Shape 2577:Mouth 2550:Other 2245:MELOS 2117:BRUIE 2094:rover 1998:rover 1976:rover 1961:rover 1836:Space 1334:Rover 1319:Flyby 1290:Orbit 1172:Earth 1094:Moons 1028:Water 904:Lakes 854:Color 704:Undae 387:(PDF) 133:genes 80:, or 52:Earth 2897:and 2715:Cell 2582:Skin 2572:Lung 2478:and 2192:THEO 1936:Mars 1867:Bion 993:Soil 738:list 567:Mars 483:2015 457:2015 403:2015 360:PMID 282:2015 274:NASA 38:Mars 30:NASA 2749:DAP 2744:NAG 2739:NAM 2567:Gut 1887:ERA 1852:BIO 397:(2) 352:doi 314:doi 129:RNA 50:of 3044:: 2919:: 2732:: 2425:: 1234:31 491:^ 474:. 447:. 393:. 389:. 372:^ 358:. 350:. 340:13 338:. 324:^ 290:^ 271:. 119:. 111:, 76:, 58:. 2996:: 2593:) 2589:( 2415:e 2408:t 2401:v 1569:e 1562:t 1555:v 1254:2 1244:7 1211:5 740:) 736:( 559:e 552:t 545:v 485:. 459:. 405:. 395:2 366:. 354:: 346:: 316:: 284:. 24:(

Index

NASA
microorganism
Mars
Bacillus pumilus
stratosphere
Earth
ionizing radiation
Microorganisms
metabolism
growth
replication
habitable zones
atmospheres
planetary protection
stratosphere
sea level
desiccation
oxidation
solar irradiation
genomic
RNA
genes
damage to the cell
genome
Bacillus pumilus


Extremophile
Interplanetary contamination
Life on Mars

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