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121:(A) The rSeVLeGFP genome expresses eGFP (239 residues) fused to the C-terminus of the L protein (2,228 residues). (B) Western blot analysis of LeGFP expressed in infected cells. Lysates of mock- (lane 1), rSeVLeGFP- (lane 2), or rSeV-eGFP- (lane 3) infected cells were reacted with anti-GFP antibody. (C) Analysis of LeGFP localization in live cells. HeLa cells were infected with rSeVLeGFP and images of the cells were captured at the indicated h p.i.
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