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57:. The test is no longer used but stands as a milestone in our understanding of immunology. A heat inactivated LGV 0.1ml grown in yolk sac of embryonated egg is injected intra dermally on the forearm and a control material prepared from noninfected yolk sac on the other forearm i.e. control. After 48–72 hours, an inflammatory nodule more than 6mm in diameter develops at the test site.
53:, produced a reaction in patients with lymphogranuloma venereum when injected intradermally. Other sources of antigen have been explored, most deriving from various tissues of mice infected with
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