207:
114:
353:
24:
348:
526:
817:
798:
646:
or DNA for
Southern blot analysis because the gel electrophoresis followed by transfer to a membrane will separate the RNA/DNA from the proteins. Additionally, since these methods use probes to bind to their conjugates, peptides that get through the process don't generally matter unless a peptide is
836:
647:
an RNase or DNase, and then only if the enzyme manages to renature, which should not occur if proper protocols are followed. A possible exception might be when working with temperature extremophiles because some enzymes of these organisms can remain stable under extraordinary circumstances.
634:
cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them. These enzymes would otherwise damage the extract.
361:
328:
463:
758:
Shimomura, O; Masugi, T; Johnson, FH; Haneda, Y (March 1978). "Properties and reaction mechanism of the bioluminescence system of the deep-sea shrimp
Oplophorus gracilorostris".
539:
451:
639:
881:
Braml, Nicole E.; Sattler, Andreas; Schnick, Wolfgang (6 February 2012). "Formation of
Melamium Adducts by Pyrolysis of Thiourea or Melamine/NH4Cl Mixtures".
256:
459:
221:
439:
926:
455:
435:
546:
405:
164:
185:
701:"The hydration structure of guanidinium and thiocyanate ions: Implications for protein stability in aqueous solution"
352:
931:
121:
71:
680:
471:
443:
347:
109:
921:
375:
340:
712:
664:
660:
467:
423:
202:
37:
604:
415:
397:
411:
427:
898:
815:, Jiawang Wu, "Production method of guanidinium thiocyanate", published 2013-04-24
775:
740:
573:
502:
890:
863:
767:
730:
720:
656:
577:
279:
796:, Rongfu Li, "Preparation method of guandine thiocyanate", published 2013-11-13
475:
173:
91:
479:
447:
47:
716:
699:
Mason, P. E.; Neilson, G. W.; Dempsey, C. E.; Barnes, A. C.; Cruickshank, J. M. (2003).
487:
206:
113:
23:
517:
389:
735:
700:
915:
643:
102:
812:
793:
495:
581:
419:
831:
153:
834:, Jianwen Fang, "Prepn of guanidyl thiocyanate", published 2004-09-08
642:. It is not strictly necessary to use phenol or chloroform if extracting RNA for
431:
624:
508:
310:
82:
725:
620:
600:
385:
902:
894:
744:
867:
779:
668:
771:
140:
122:
852:"CL. – Guanidine thiocyanate: its formation from ammonium thiocyanate"
655:
This substance can be prepared by reacting guanidinium carbonate with
851:
516:
Except where otherwise noted, data are given for materials in their
381:
631:
616:
393:
70:
60:
491:
589:
585:
483:
190:
599:. This is because guanidinium is the conjugate acid of
534:
640:
guanidinium thiocyanate-phenol-chloroform extraction
615:
Guanidinium thiocyanate can be used to deactivate a
230:InChI=1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
240:InChI=1/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
152:
705:Proceedings of the National Academy of Sciences
663:under heat. Another method is the pyrolysis of
46:
576:used as a general protein denaturant, being a
8:
205:
112:
90:
15:
734:
724:
580:, although it is most commonly used as a
172:
630:Guanidinium thiocyanate is also used to
691:
261:
226:
201:
627:", so that it can be studied safely.
103:
233:Key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N
7:
243:Key: ZJYYHGLJYGJLLN-UHFFFAOYAS
143:
14:
524:
351:
346:
297:
291:
22:
595:GITC may also be recognized as
584:protector in the extraction of
520:(at 25 °C , 100 kPa).
883:Chemistry – A European Journal
303:
285:
1:
948:
638:A commonly used method is
566:guanidinium isothiocyanate
514:
327:
322:
272:
252:
217:
30:
21:
406:Precautionary statements
17:Guanidinium thiocyanate
726:10.1073/pnas.0735920100
681:Guanidine hydrochloride
559:Guanidinium thiocyanate
895:10.1002/chem.201101885
623:that caused the 1918 "
927:Guanidinium compounds
597:guanidine thiocyanate
868:10.1039/CT9130301378
856:J. Chem. Soc., Trans
850:Krall, Hans (1913).
665:ammonium thiocyanate
661:ammonium thiocyanate
772:10.1021/bi00599a008
717:2003PNAS..100.4557M
318: g·mol
18:
605:guanidinium cation
603:and is called the
547:Infobox references
16:
932:Chaotropic agents
644:Northern blotting
574:chemical compound
555:Chemical compound
553:
552:
503:Safety data sheet
376:Hazard statements
186:CompTox Dashboard
72:Interactive image
939:
907:
906:
878:
872:
871:
847:
841:
840:
839:
835:
828:
822:
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820:
816:
809:
803:
802:
801:
797:
790:
784:
783:
755:
749:
748:
738:
728:
711:(8): 4557–4561.
696:
657:ammonium sulfate
578:chaotropic agent
537:
531:
528:
527:
497:
493:
489:
485:
481:
477:
473:
469:
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417:
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395:
391:
387:
383:
355:
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317:
305:
299:
293:
287:
280:Chemical formula
210:
209:
194:
192:
176:
156:
145:
124:
116:
105:
94:
74:
50:
26:
19:
947:
946:
942:
941:
940:
938:
937:
936:
912:
911:
910:
880:
879:
875:
849:
848:
844:
837:
830:
829:
825:
818:
811:
810:
806:
799:
792:
791:
787:
757:
756:
752:
698:
697:
693:
689:
677:
653:
621:influenza virus
613:
556:
549:
544:
543:
542: ?)
533:
529:
525:
521:
408:
378:
364:
343:
315:
302:
296:
290:
282:
268:
265:
260:
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213:
195:
188:
179:
159:
146:
134:
97:
77:
64:
53:
40:
12:
11:
5:
945:
943:
935:
934:
929:
924:
914:
913:
909:
908:
889:(6): 1811–19.
873:
842:
823:
804:
785:
750:
690:
688:
685:
684:
683:
676:
673:
652:
649:
619:, such as the
612:
609:
554:
551:
550:
545:
523:
522:
518:standard state
515:
512:
511:
506:
499:
498:
464:P305+P351+P338
452:P303+P361+P353
444:P301+P330+P331
409:
404:
401:
400:
379:
374:
371:
370:
365:
360:
357:
356:
344:
339:
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307:
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278:
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255:
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239:
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229:
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220:
219:
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198:
196:
184:
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180:
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177:
169:
167:
161:
160:
158:
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149:
147:
139:
136:
135:
133:
132:
128:
126:
118:
117:
107:
99:
98:
96:
95:
87:
85:
79:
78:
76:
75:
67:
65:
58:
55:
54:
52:
51:
43:
41:
36:
33:
32:
28:
27:
13:
10:
9:
6:
4:
3:
2:
944:
933:
930:
928:
925:
923:
920:
919:
917:
904:
900:
896:
892:
888:
884:
877:
874:
869:
865:
861:
857:
853:
846:
843:
833:
827:
824:
814:
808:
805:
795:
789:
786:
781:
777:
773:
769:
766:(6): 994–98.
765:
761:
754:
751:
746:
742:
737:
732:
727:
722:
718:
714:
710:
706:
702:
695:
692:
686:
682:
679:
678:
674:
672:
670:
666:
662:
658:
650:
648:
645:
641:
636:
633:
628:
626:
622:
618:
610:
608:
606:
602:
598:
593:
591:
587:
583:
579:
575:
571:
567:
563:
560:
548:
541:
536:
519:
513:
510:
509:External MSDS
507:
504:
501:
500:
410:
407:
403:
402:
380:
377:
373:
372:
369:
366:
363:
359:
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333:
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321:
314:
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309:
308:
284:
281:
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216:
208:
204:
203:DTXSID6060478
200:
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187:
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163:
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155:
151:
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148:
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138:
137:
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125:
120:
119:
115:
111:
108:
106:
104:ECHA InfoCard
101:
100:
93:
89:
88:
86:
84:
81:
80:
73:
69:
68:
66:
62:
57:
56:
49:
45:
44:
42:
39:
35:
34:
29:
25:
20:
922:Thiocyanates
886:
882:
876:
859:
855:
845:
826:
813:CN 103058889
807:
794:CN 103387520
788:
763:
760:Biochemistry
759:
753:
708:
704:
694:
654:
637:
629:
614:
596:
594:
592:from cells.
582:nucleic acid
569:
565:
561:
558:
557:
367:
329:
31:Identifiers
862:: 1378–91.
651:Preparation
625:Spanish flu
362:Signal word
273:Properties
264:N#CS.=C(N)N
110:100.008.922
916:Categories
832:CN 1235876
687:References
671:at 180°C.
341:Pictograms
311:Molar mass
174:GCA2EU297U
83:ChemSpider
59:3D model (
38:CAS Number
601:guanidine
460:P304+P340
456:P304+P312
448:P302+P352
440:P301+P312
332:labelling
131:209-812-1
123:EC Number
903:22223531
745:12684536
675:See also
669:thiourea
323:Hazards
48:593-84-0
713:Bibcode
572:) is a
540:what is
538: (
141:PubChem
901:
838:
819:
800:
780:629957
778:
743:
736:404697
733:
535:verify
532:
505:(SDS)
368:Danger
316:118.16
257:SMILES
617:virus
562:(GTC)
222:InChI
154:65046
92:58557
61:JSmol
899:PMID
776:PMID
741:PMID
632:lyse
611:Uses
607:, .
588:and
570:GITC
496:P501
492:P405
488:P363
484:P330
480:P322
476:P321
472:P312
468:P310
436:P280
432:P273
428:P271
424:P270
420:P264
416:P261
412:P260
398:H412
394:H332
390:H314
386:H312
382:H302
165:UNII
891:doi
864:doi
860:103
768:doi
731:PMC
721:doi
709:100
667:or
659:or
590:RNA
586:DNA
564:or
330:GHS
191:EPA
144:CID
918::
897:.
887:18
885:.
858:.
854:.
774:.
764:17
762:.
739:.
729:.
719:.
707:.
703:.
494:,
490:,
486:,
482:,
478:,
474:,
470:,
466:,
462:,
458:,
454:,
450:,
446:,
442:,
438:,
434:,
430:,
426:,
422:,
418:,
414:,
396:,
392:,
388:,
384:,
334::
905:.
893::
870:.
866::
782:.
770::
747:.
723::
715::
568:(
530:Y
304:S
301:4
298:N
295:6
292:H
289:2
286:C
193:)
189:(
63:)
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