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Drops of glowing fluid from a glow stick are added to a chalk so that a band is created halfway through it. The chalk is then placed vertically into a beaker filled with a small amount of acetone or alcohol - ensuring the surface of the solvent is below the dye band. The liquid is then allowed to
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travel up the chalk; polar dyes would tend to stick to the chalk and not travel significantly while non-polar dyes would travel up with the solvent. Once it travels almost to the top of the chalk, it is removed from the beaker. The chalk
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travels up the chalk. 2: Separation starts as the red dye is attracted to the mobile phase and starts traveling up while the blue dye is attracted to the stationary phase and stays relatively still. 3: Complete separation occurs as time
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Thomas S. Kuntzleman, Anna E. Comfort, Bruce W. Baldwin. (2019). "Simple
Glowmatography: Chromatographic Separation of Glow-Stick Dyes Using Chalk".
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components. Developed as a laboratory class experiment, it can be used to demonstrate chemistry concepts of polarity,
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This experiment can be conducted with glow sticks, chalks, and solutions of acetone or isopropyl alcohol.
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Additionally, this glomatographic experiment can be done using other materials. For instance,
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Thomas S. Kuntzleman, Anna E. Comfort, Bruce W. Baldwin. (2009). "Glowmatography".
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Demonstration of glowmatography; experiment done over a span of about half an hour.
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acting as the mobile phase. The polar components would be attracted to the polar
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can be used as the stationary phase together with a solution of nonpolar
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Glow stick solutions and chalk sticks - preparation for experiment
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Bruce W. Baldwin, Kasey R. Bunker, Thoman S. Kuntzleman (2019).
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103:), and therefore contains ions. This allows it to attract other
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383:"Extraction of Dyes Contained in Glow Sticks Using Liquid CO
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In the chromatography of a glow stick solution, a piece of
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the chalk. This then allows for the separation of dyes.
340:"Mini-Encyclopedia of Papermaking Wet-End Chemistry"
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195:and other dyes would be attracted to cotton.
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177:silanol
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