78:
22:
700:
Neudorfer, J; Schmidt, B; Huster, K. M.; Ander, F.; Schiemann, M.; Holzapfel, G.; Schmidt, T.; Germeroth, L.; Wagner, H.; Peschel, C.; Busch, D. H.; Bernhard, H. (2007). "Reversible HLA multimers (Streptamers) for the isolation of human cytotoxic T lymphocytes functionally active against tumor- and
233:
and other cells of interest. This technology is known as Fab-TACS (Traceless
Affinity Cell Selection) and is based on Strep-tagged Fab fragments, which reversibly capture and release the target cells. This delivers label-free, non-activated cells suitable for immunologic or cell biological assays.
234:
The technology can be used with manual gravity flow columns as well as with the automated cell selection device FABian. Isolated and purified cells are being deployed for basic research as well as diagnostic applications. The
Streptamers for cell expansion enable the controlled stimulation of
292:
of interest with an extremely high affinity. This results in improved batch purification runs, higher yields of the expressed recombinant protein and a versatile approach for the immobilization on solid surfaces like beads, slides/arrays or microplates.
646:
1136:
Knabel, M.; Franz, T. J.; Schiemann, M.; Wulf, A.; Villmow, B.; Schmidt, B.; Bernhard, H.; Wagner, H.; Busch, D. H. (2002). "Reversible MHC multimer staining for functional isolation of T-cell populations and effective adoptive transfer".
632:
199:
towards Strep-Tactin results in a highly specific interaction, which enables the isolation and purification of sensitive proteins in a native state as wells as intact protein complexes, respectively. The technology was patented by the
238:. They consist of non-magnetic soluble protein complexes generated by multimerization of αCD3- and αCD28 Fab-Streps with a Strep-Tactin multimer. The reversible reagents can be easily removed from the cells by the addition of
1054:
Pelák, O.; KuĹľĂlková, D.; ThĂĽrner, D.; Kiene, M. L.; Stanar, K.; StuchlĂ˝, J.; Vášková, M.; StarĂ˝, J.; Hrušák, O.; Stadler, H.; Kalina, T. (2016). "Lymphocyte enrichment using CD81-targeted immunoaffinity matrix".
680:
Pelák, O.; KuĹľĂlková, D.; ThĂĽrner, D.; Kiene, M. L.; Stanar, K.; StuchlĂ˝, J.; Vášková, M.; StarĂ˝, J.; Hrušák, O.; Stadler, H.; Kalina, T. (2016). "Lymphocyte enrichment using CD81-targeted immunoaffinity matrix".
660:
Pelák, O.; KuĹľĂlková, D.; ThĂĽrner, D.; Kiene, M. L.; Stanar, K.; StuchlĂ˝, J.; Vášková, M.; StarĂ˝, J.; Hrušák, O.; Stadler, H.; Kalina, T. (2016). "Lymphocyte enrichment using CD81-targeted immunoaffinity matrix".
1088:
171:
company providing products and custom specific services for life science applications in academia and industry worldwide. IBA focusses on two business segments: cell selection and protein purification.
204:(former “Garching Innovations”) and later assigned to IBA. Recently, the method was further developed to be used for cell selection from whole blood or other single cell suspensions.
981:
Witte, C. P.; Noël, L. D.; Gielbert, J.; Parker, J. E.; Romeis, T. (2004). "Rapid one-step protein purification from plant material using the eight-amino acid StrepII epitope".
835:
Schmidt, T.; Skerra, A. (1994). "One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin".
1102:
493:
402:
In 2011 IBA was granted multiple local innovation awards, e.g. for its
Streptamer technology, which allows reversible isolation and staining of antigen-specific
870:
Schmidt, T.; Koepke, J.; Frank, R.; Skerra, A. (1996). "Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin".
1234:
426:). The officers of the company are Dr. Herbert Stadler (Founder and President), Dr. Mike Rothe (CEO) and Dr. Joachim Bertram (CSO). Headquartered in
277:
This can be used for i.e. drug screening, diagnostic assays, immobilization and interaction studies. Due to its small size and biochemically almost
957:
608:
1074:
406:. Additionally, a diagnostic test developed by IBA and Fassisi for veterinary diseases was chosen as one of the top five innovations by the
785:
Schmidt, T. G.; Skerra, A. (2007). "The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins".
556:
Schmidt, T. G.; Skerra, A. (2007). "The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins".
521:
Schmidt, T. G.; Skerra, A. (2007). "The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins".
940:
Skerra, A.; Schmidt, T. G. (2000). "Use of the Strep- tag and streptavidin for detection and purification of recombinant proteins".
591:
Skerra, A.; Schmidt, T. G. (2000). "Use of the Strep- tag and streptavidin for detection and purification of recombinant proteins".
222:
1214:
374:- IBA earned its ISO 9001:2008 certification for Quality Management Standard, IBA introduces Strep-Tactin XT products
1026:
288:
The latest generation of the system is called Strep-Tactin XT. In combination with the Twin Strep-tag it binds the
259:
242:. This allows to regulate the extent of stimulation precisely and to study functional and label-free activated
187:. The method is based on one of the strongest non-covalent interactions in nature, which is the interaction of
1172:
407:
218:
180:
410:
in 2015. Furthermore, the company honors young scientists and participated in research consortia as well.
263:
184:
905:
Skerra, A.; Schmidt, T. (1999). "Applications of a peptide ligand for streptavidin: the Strep-tag".
289:
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32:
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77:
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The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture
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Use of the strep-tag and streptavidin for detection and purification of recombinant proteins
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Use of the strep-tag and streptavidin for detection and purification of recombinant proteins
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647:"United States Patent - Peptides with sequentially arranged streptavidin binding modules"
762:
737:
473:
51:
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451:
368:- IBA introduces Fab-TACS (Formerly known as T-CATCH) column based cell purification
168:
88:
381:
192:
738:"T cells for viral infections after allogeneic hematopoietic stem cell transplant"
427:
115:
1201:"Bundesministerium fĂĽr Bildung und Forschung - Forschungsschwerpunkt Biophotonik"
753:
304:- Institut fĂĽr Bioanalytik GmbH was founded by Herbert Stadler, IBA introduces
994:
714:
468:
156:
447:
338:- Operations segmented into Protein, Gene and Cell TAGnology business units
305:
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633:"United States Patent - Streptavidin-binding peptide and uses thereof"
439:
239:
188:
314:- Acquisition of NAPS Göttingen GmbH (Nucleic Acid Products Supply)
463:
1186:
419:
418:
IBA Lifesciences is a limited liability company (referred to as
15:
362:- Innovation Award for Streptamer cell isolation technology
175:
The company is the original manufacturer and supplier of the
285:
does not influence protein folding, secretion and function.
1117:"Innovationspreis 2011 des Landkreises Göttingen - Youtube"
1089:"Research in Germany - Foreign Trade Award of Lower Saxony"
326:- Institut fĂĽr Bioanalytik GmbH was renamed to IBA GmbH
46:
36:
332:- IBA introduces Streptamer cell isolation technology
254:
The company exhibits a wide range of tools related to
1075:"IBA GmbH Certified with ISO 9001:2008 Accreditation"
944:. Methods Enzymol. Vol. 326. pp. 271–304.
595:. Methods Enzymol. Vol. 326. pp. 271–304.
824:. Methods Mol Biol. Vol. 1286. pp. 83–95.
152:
142:
126:
110:
102:
94:
84:
1173:"Innovationspreis 2017 des Landkreises Göttingen"
1027:"Bionity - Trennsysteme fĂĽr den Pharma Weltmarkt"
388:), IBA launches MEXi mammalian expression system
320:- Biologics joint venture with GBF Braunschweig
394:- IBA introduces cell selection device FABian
1103:"Göttinger Tageblatt - Außenwirtschaftspreis"
494:"Göttinger Tageblatt - Außenwirtschaftspreis"
132:Dr. Herbert Stadler (Founder & President)
8:
70:
356:- IBA introduces Fab-Streptamer technology
1041:"faktor - Innovationspreis 2008 verliehen"
76:
69:
1002:
761:
344:- IBA introduces StarGate cloning system
485:
195:. An intrinsic binding affinity of the
736:Bollard, C. M.; Heslop, H. E. (2016).
508:"VBio - 10 Jahre IBA. Ein Kurzporträt"
474:Cell isolation with Fab-TACS® Gravity
469:Strep-tag® protein purification cycle
221:system for non-magnetic isolation of
7:
1235:Biotechnology companies of Germany
223:peripheral blood mononuclear cells
14:
1215:"Bionity - EU Forschungsvorhaben"
350:- Innovation Award for StarGate
820:Schmidt, T.; Skerra, A. (2015).
20:
464:http://www.iba-lifesciences.com
250:Protein Production & Assays
1004:11858/00-001M-0000-0012-3BE7-1
213:Cell Selection & Expansion
1:
950:10.1016/S0076-6879(00)26060-6
919:10.1016/S1050-3862(99)00033-9
601:10.1016/S0076-6879(00)26060-6
380:- 6th foreign Trade Award of
179:/Strep-Tactin technology, an
849:10.1016/0021-9673(94)80434-6
754:10.1182/blood-2016-01-628982
258:purification consisting of
1251:
995:10.1007/s11103-004-0501-y
715:10.1016/j.jim.2007.01.001
701:virus-derived antigens".
208:Products and technologies
138:Dr. Joachim Bertram (CSO)
75:
398:Awards & Sponsorship
157:www.iba-lifesciences.com
219:affinity chromatography
181:affinity chromatography
35:, as no other articles
884:10.1006/jmbi.1996.0061
799:10.1038/nprot.2007.209
570:10.1038/nprot.2007.209
535:10.1038/nprot.2007.209
274:/Strep-Tactin system.
264:affinity purification
217:IBA has developed an
183:system developed for
185:protein purification
135:Dr. Mike Rothe (CEO)
1187:"FDN 2016 - Awards"
1125:. 24 November 2011.
408:county of Göttingen
290:recombinant protein
256:recombinant protein
144:Number of employees
106:Dr. Herbert Stadler
72:
1151:10.1038/nm0602-631
434:with locations in
260:expression vectors
202:Max Planck Society
54:for suggestions.
44:to this page from
959:978-0-12-182227-9
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703:J Immunol Methods
610:978-0-12-182227-9
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1057:Cytometry A
683:Cytometry A
663:Cytometry A
448:Saint Louis
59:August 2023
907:Biomol Eng
872:J Mol Biol
787:Nat Protoc
558:Nat Protoc
523:Nat Protoc
480:References
128:Key people
50:; try the
37:link to it
428:Göttingen
414:Structure
308:products
306:Strep-tag
283:Strep-tag
272:Strep-tag
197:Strep-tag
177:Strep-tag
116:Göttingen
40:. Please
1229:Category
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444:Germany
436:Leipzig
432:Germany
424:Germany
404:T cells
386:Germany
297:History
244:T cells
236:T cells
231:B cells
227:T cells
153:Website
121:Germany
103:Founder
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61:)
57:(
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