221:, which cannot be related to an identifiable cause but are building related. Over the last two decades, there have been many studies indicating association of indoor bioaerosol with sick building syndrome. However, most of the related studies based their conclusions on statistical correlation between concentrations of certain types of bioaerosols and incidence of complaints, which has various drawbacks methodologically. For example, some studies have a small sample size, which critically undermines the validity of speculations based on the statistical results. Also, many studies were not able to exclude the influences of other factors beside bioaerosol in their analysis, which makes the statistical correlation theoretically inappropriate to support association of SBS with bioaerosols. Additional studies revealed that bioaerosol is unlikely to be the cause of SBS. Recent epidemiological and toxicological studies continued to suggest a possible link between bioaerosol exposure and sick building syndrome, but methodological limitations remained in these studies.
372:., FISH method successfully identified eubacteria in samples of complex native bioaerosols in swine barns. Nonetheless, molecular biological tools have limitations. Since PCR methods target DNA, viability of cells could not be confirmed in some cases. When qPCR technique is used for bioaerosol detection, standard curves need to be developed to calibrate final results. One study indicated that "curves used for quantification by qPCR needs to be prepared using the same environmental matrix and procedures as handling of the environmental sample in question" and that "reliance on the standard curves generated with cultured bacterial suspension (a traditional approach) may lead to substantial underestimation of microorganism quantities in environmental samples". Microarray techniques also face the challenge of natural sequence diversity and potential cross-hybridisation in complex environmental bioaerosols).
277:, bioaerosols need to be captured from the air first. Different air sampling techniques have been used to realise the goal of capturing indoor bioaerosols.. Important characteristics of bioaerosol sampling include: representativeness of sampling, sampler performance, and compatibility with subsequent analysis. Long-term sampler theoretically has a better representativeness of sampling than short-term sampler, but may not have a good temporary resolution. Performance of samplers (i.e., limit of detection and upper limit of range) has a significant impact on the reliability of results. Different characterisations of samplers can also limit the possibilities for further analysis (identification and quantification). Major bioaerosol sampler types and their possible subsequent analysis are summarised in Table 1. A frequently used sampler in previous studies is the Andersen impactor.
352:
underestimate environmental microbial diversity, based on the fact that only a small percentage of microbes can be cultivated in the laboratory. This underestimation is likely to be signified for the quantification of bioaerosol, since colony counts of airborne microbes are typically quite different from direct counts. Culture-based methods also need relatively long incubation times (over 24 hours) and are labor-intensive. Consequently, culture-based methods are no longer suitable for effective and rapid identification and quantification of bioaerosol, and non-culture based methods, such as immunoassays, molecular biological tests, and optical, and electrical methods, have been developing over the past few decades.
561:(<60%); 3) installing appropriate filtration devices to air ventilation system to inlet filtered outdoor air into indoor environment; 4) reducing/removing contaminant sources (i.e., indoor organic waste). As in the U.S., due to the increase in tuberculosis in the mid-1980s, indoor air treatment has developed substantially during the past two decades. Current or developing indoor air purification technologies include filtration, aerosol ultraviolet irradiation, electrostatic precipitation, unipolar ion emission, and photocatalytic oxidation.
356:
immunoassay (i.e., enzyme-linked immunosorbent assay (ELISA)). The well-known PCR is a powerful tool in identifying and even quantifying the biological origin of bioaerosols. PCR alone cannot accomplish all the tasks related to bioaerosol detection; instead it usually serves as the preparation tool for subsequent processes like DNA sequencing, microarray, and community fingerprinting techniques. A typical procedure for PCR-based bioaerosol analysis is shown in Figure 1.
360:
145:, geographical factors, do not seem to have significant impacts on respirable fungi and bacteria (important constituents of bioaerosols). Relative humidity is one of the most widely studied influencing factors for indoor bioaerosols. Concentrations of two categories of bioaerosols, endotoxin and airborne fungi, are both positively related to indoor relative humidity (higher concentration associated with higher relative humidity). Relative humidity also affects the
105:., they investigated the effects of indoor storage of organic household waste on microbial contamination among 99 households in the Netherlands in the summer of 1997, and indicated that "increased microbial contaminant levels in homes are associated with indoor storage of separated organic waste", which might elevate "the risk of bioaerosol-related respiratory symptoms in susceptible people". However, the analysis by Wouters
343:
concentrations are on the order of 10/cm or greater, bioaerosol concentrations are commonly less than 1/cm, and often less than 1/m in the case of infectious aerosols. Moreover, many commercially available bioaerosol samplers haven not been investigated on their collection efficiencies for particles with different aerodynamic diameters, which makes it impossible to get the size-resolved bioaerosol information.
260:(ACGIH)) with the culturable count for total bacteria not exceeding 500 CFUs/m. Note that for most types of indoor bioaerosols, the establishment of specific concentration limits or acceptance levels presents multiple challenges (e.g., differences on sampling and analysis method, irrelevance of sampling units to human exposure measurement; multiplicity and variability of composition, etc.).
97:) system. Several studies have identified human activities as an important source for indoor bioaerosols. Human bodies can generate bioaerosols directly through activities like talking, sneezing, and coughing, while other residential activities (i.e., washing, flushing toilet, sweeping floor) can generate bioaerosols indirectly. Since microorganisms can accumulate and grow on
342:
Certain limitations exist for commonly used bioaerosol samplers. For most of the samplers, nonbiological environmental particles such as dust must be separated from bioaerosols prior to detection. The diluted nature of bioaerosol in the air also poses challenges to samplers. While total microorganism
351:
In previous research on indoor bioaerosol in residential environments, microorganisms have been quantified by conventional culture-based techniques, in which colony forming units (CFU) on selective media are counted. Cultivating methods have several disadvantages. Culture-based methods are known to
367:
Molecular biological methods for bioaerosol are significantly faster and more sensitive than conventional culture-based methods, and they are also able to reveal a larger diversity of microbes. Targeting the variation in the 16S rRNA gene, a microarray (PhyloChip) was used to conduct comprehensive
355:
Major culture-independent identification/quantification methods adopted in previous bioaerosol studies include polymerase chain reaction (PCR), quantitative polymerase chain reaction (qPCR), microarray (PhyloChip), fluorescent in situ hybridisation (FISH), flow cytometry and solid-phase cytometry,
157:
Adverse health effects/diseases related to indoor bioaerosol exposure can be divided into two categories: those confirmed to be associated with bioaerosol and those suspected but not confirmed to be associated with bioaerosol. Bioaerosols have been revealed to cause certain human diseases, such as
85:, certain categories of outdoor bioaerosols (i.e., fungal spores) do serve as major sources for indoor bioaerosols in naturally ventilated buildings at specific periods of time (i.e., growing seasons for fungi). Major indoor sources for bioaerosols at residential homes include human occupants,
560:
Based on the sources and the influencing factors for indoor bioaerosols, corresponding remedial actions can be taken to control related contamination. Potentially effective strategies include: 1) limiting entrance of outdoor aerosols; 2) keeping the relative humidity level below high levels
63:
as small as 1–10 μm in size. Since bioaerosols are potentially related to various human health effects and the indoor environment provides a unique exposure situation, concerns about indoor bioaerosols have increased over the last decade.
251:
Because of the confirmed and potential adverse health effects associated with indoor bioaerosol, some concentration limits for total number of bioaerosol particles are recommended by different agencies and organisations as follow: 1000 CFUs/m
862:
Peccia, J. and M. Hernandez, Incorporating polymerase chain reaction-based identification, population characterization, and quantification of microorganisms into aerosol science: A review. Atmospheric
Environment, 2006. 40(21): p.
919:
Teeuw, K.B., C. Vandenbrouckegrauls, and J. Verhoef, Airborne gram-negative bacteria and endotoxin in sick building syndrome - a study in Dutch governmental office buildings. Archives of
Internal Medicine, 1994. 154(20): p.
186:. Bioaerosols are also associated with some noninfectious airway diseases, such as allergies and asthma. As a known component of indoor bioaerosol, β(1→3)-glucan (cell wall components of most fungi) is proposed to be the
109:. was based on the collected samples of settled house dust, which might not serve as a strong indicator for bioaerosols suspended in the air. Other materials in residential buildings, such as food stuffs, house plants,
879:
Vanhee, L.M.E., H.J. Nelis, and T. Coenye, Rapid
Detection and Quantification of Aspergillus fumigatus in Environmental Air Samples Using Solid-Phase Cytometry. Environmental Science & Technology, 2009. 43(9): p.
1069:
Lange, J.L., P.S. Thorne, and N. Lynch, Application of flow cytometry and fluorescent in situ hybridisation for assessment of exposures to airborne bacteria. Applied and
Environmental Microbiology, 1997. 63(4): p.
1010:
Moon, H.S., et al., Dielectrophoretic
Separation of Airborne Microbes and Dust Particles Using a Microfluidic Channel for Real-Time Bioaerosol Monitoring. Environmental Science & Technology, 2009. 43(15): p.
113:, wood material and furniture stuffing can also become bioaerosol sources when water content is appropriate for microorganisms to grow. For non-residential buildings, some specific indoor environments, such as
368:
identification of both bacterial and archaeal organisms in bioaerosols. New U.S. EPA methods have been developed to utilise qPCR to characterise indoor environment for fungal spores. In a study by Lange
795:
Sanchez-Monedero, M.A., E.I. Stentiford, and C. Mondini, Biofiltration at composting facilities: Effectiveness for bioaerosol control. Environmental
Science & Technology, 2003. 37(18): p. 4299-4303.
740:
Chen, Q. and L.M. Hildemann, The
Effects of Human Activities on Exposure to Particulate Matter and Bioaerosols in Residential Homes. Environmental Science & Technology, 2009. 43(13): p. 4641-4646.
994:
Gorny, R.L. and J. Dutkiewicz, Bacterial and fungal aerosols in indoor environment in
Central and Eastern European countries. Annals of Agricultural and Environmental Medicine, 2002. 9(1): p. 17-23.
832:
Dekoster, J.A. and P.S. Thorne, Bioaerosol concentrations in noncomplaint, complaint, and intervention homes in the
Midwest. American Industrial Hygiene Association Journal, 1995. 56(6): p. 573-580.
1041:
An, H.R., G. Mainelis, and L. White, Development and calibration of real-time PCR for quantification of airborne microorganisms in air samples. Atmospheric
Environment, 2006. 40(40): p. 7924-7939.
708:
Wouters, I.M., et al., Increased levels of markers of microbial exposure in homes with indoor storage of organic household waste. Applied and Environmental Microbiology, 2000. 66(2): p. 627-631.
1055:
Brodie, E.L., et al., Urban aerosols harbor diverse and dynamic bacterial populations. Proceedings of the National Academy of Sciences of the United States of America, 2007. 104(1): p. 299-304.
149:
of airborne viruses. Regarding the characterisation of air ventilation system, increased use of central air conditioning is found to be associated with lower fungal bioaerosol concentration.
59:, etc. Size of bioaerosol particles varies from below 1 μm to 100 μm in aerodynamic diameter; viable bioaerosol particles can be suspended in air as single cells or aggregates of
610:
Sanchez-Monedero, M.A., et al., Effect of the aeration system on the levels of airborne microorganisms generated at wastewater treatment plants. Water Research, 2008. 42(14): p. 3739-3744.
257:
929:
Li, C.S., C.W. Hsu, and M.L. Tai, Indoor pollution and sick building syndrome symptoms among workers in day-care centers. Archives of Environmental Health, 1997. 52(3): p. 200-207.
690:
Peccia, J., et al., A role for environmental engineering and science in preventing bioaerosol-related disease. Environmental Science & Technology, 2008. 42(13): p. 4631-4637.
253:
1079:
Wan, G.H. and C.S. Li, Indoor endotoxin and glucan in association with airway inflammation and systemic symptoms. Archives of Environmental Health, 1999. 54(3): p. 172-179.
232:
and biological characteristics, but also on the quantity of bioaerosol inhaled and their size distribution, which determines the site of bioaerosol deposition to human
898:
Cooley, J.D., et al., Correlation between the prevalence of certain fungi and sick building syndrome. Occupational and Environmental Medicine, 1998. 55(9): p. 579-584.
846:
Law, A.K.Y., C.K. Chau, and G.Y.S. Chan, Characteristics of bioaerosol profile in office buildings in Hong Kong. Building and Environment, 2001. 36(4): p. 527-541.
1029:
Fierer, N., et al., Short-term temporal variability in airborne bacterial and fungal populations. Applied and Environmental Microbiology, 2008. 74(1): p. 200-207.
1088:
Tsai, F.C. and J.M. Macher, Concentrations of airborne culturable bacteria in 100 large US office buildings from the BASE study. Indoor Air, 2005. 15: p. 71-81.
804:
Bauer, H., et al., Bacteria and fungi in aerosols generated by two different types of wastewater treatment plants. Water Research, 2002. 36(16): p. 3965-3970.
201:
Some other human diseases and symptoms have been proposed to be associated with indoor bioaerosol, but no deterministic conclusions could be drawn due to the
198:
cases in industrialised countries are induced by fungi, which has been the focus of concerns about human exposure to airborne microorganisms in recent years.
1097:
Jo, W.K. and Y.J. Seo, Indoor and outdoor bioaerosol levels at recreation facilities, elementary schools, and homes. Chemosphere, 2005. 61(11): p. 1570-1579.
772:
Kalogerakis, N., et al., Indoor air quality - bioaerosol measurements in domestic and office premises. Journal of Aerosol Science, 2005. 36(5-6): p. 751-761.
363:
Figure 1 Pathways to PCR-based bioaerosol analysis (adapted from).The numbers listed indicate the quantities of bioaerosols required for successful analysis.
816:
Verreault, D., S. Moineau, and C. Duchaine, Methods for sampling of airborne viruses. Microbiology and Molecular Biology Reviews, 2008. 72(3): p. 413-444.
668:
Chao, H.J., et al., Populations and determinants of airborne fungi in large office buildings. Environmental Health Perspectives, 2002. 110(8): p. 777-782.
640:
Pastuszka, J.S., et al., Bacterial and fungal aerosol in indoor environment in Upper Silesia, Poland. Atmospheric Environment, 2000. 34(22): p. 3833-3842.
910:
Gyntelberg, F., et al., Dust and the sick building syndrome. Indoor Air-International Journal of Indoor Air Quality and Climate, 1994. 4(4): p. 223-238.
125:
facilities, certain biotechnical laboratories, have been revealed to have bioaerosol sources related to their particular environmental characteristics.
598:
Douwes, J., et al., Bioaerosol health effects and exposure assessment: Progress and prospects. Annals of Occupational Hygiene, 2003. 47(3): p. 187-200.
726:
Burge, H., Bioaerosol - prevalence and health effects in the indoor environment. Journal of Allergy and Clinical Immunology, 1990. 86(5): p. 687-701.
81:
may originate from outdoor air and indoor reservoirs. Although outdoor bioaerosols cannot easily migrate into large buildings with complex
380:
Concentration levels of indoor bioaerosols in different regions of the world recorded in published literatures are summarised as Table 2.
244:, and particles with aerodynamic diameter less than 5 μm can reach the alveoli and hence lead to serious illnesses such as allergic
786:
Li, C.S. and P.A. Hou, Bioaerosol characteristics in hospital clean rooms. Science of the Total Environment, 2003. 305(1-3): p. 169-176.
31:, plant, or animal) origin suspended in the air. These particles are also referred to as organic dust. Bioaerosols may consist of
1020:
Li, C.S. and T.Y. Huang, Fluorochrome in monitoring indoor bioaerosols. Aerosol Science and Technology, 2006. 40(4): p. 237-241.
754:
Park, J.H., et al., Predictors of airborne endotoxin in the home. Environmental Health Perspectives, 2001. 109(8): p. 859-864.
1122:
245:
1112:
133:
According to previous studies, major indoor environmental factors influencing bioaerosol concentration include relative
240:, those between 5-10 μm mainly deposit in the upper respiratory system and usually induce symptoms like allergic
82:
1117:
183:
274:
202:
982:
Pasanen, A.L., A review: Fungal exposure assessment in indoor environments. Indoor Air, 2001. 11(2): p. 87-98.
236:. Bioaerosols larger than 10 μm in aerodynamic diameter are generally blocked by the nasal region of the
163:
580:
206:
889:
Redlich, C.A., J. Sparer, and M.R. Cullen, Sick-building syndrome. Lancet, 1997. 349(9057): p. 1013-1016.
938:
Burge, P.S., Sick building syndrome. Occupational and Environmental Medicine, 2004. 61(2): p. 185-190.
229:
118:
947:
Harrison, J., et al., An investigation of the relationship between microbial and particulate indoor
167:
575:
233:
171:
138:
359:
237:
214:
142:
209:(SBS). SBS refers to non-specific complaints, such as upper-respiratory irritative symptoms,
141:, temperature, and chemical composition of the air. Other factors, such as the type of home,
48:
187:
194:-induced nonallergic inflammatory reactions. It is reported that 25%-30% of allergenic
1106:
948:
191:
90:
60:
961:
159:
960:
Laumbach, R.J. and H.M. Kipen, Bioaerosols and sick building syndrome: particles,
101:
particles, house dust is a potential source of bioaerosols. In a study by Wouters
146:
56:
384:
Table 2 Concentration of indoor bioaerosols in different regions of the world
951:
and the sick building syndrome. Respiratory Medicine, 1992. 86(3): p. 225-235.
570:
78:
20:
968:. Current Opinion in Allergy and Clinical Immunology, 2005. 5(2): p. 135-139.
175:
519:
Highest bacterial concentration: 2912; Highest fungal concentration: 3852.
241:
210:
134:
114:
32:
24:
965:
456:
Total bacteria (average): 101.9; Total bacteria (90th percentile): 175.
437:
Bacteria: 7651(DC), 1502(OB), 2907(H); Fungi: 854(DC), 195(OB), 695(H).
225:
179:
122:
110:
28:
336:
Cultivation; Microscopic analysis; Biochemical analysis; Immunoassays
325:
Cultivation; Microscopic analysis; Biochemical analysis; Immunoassays
314:
Cultivation; Microscopic analysis; Biochemical analysis; Immunoassays
195:
52:
36:
475:
Bacterial aerosol in homes: 1000; Bacterial aerosol in offices: 100.
358:
44:
40:
218:
98:
94:
23:
in an indoor environment. Bioaerosols are natural or artificial
434:
40 daycare centers (DC), 69 office buildings (OB), 22 homes (H)
311:
AGI-30; Shipe sampler; Midget, multi-stage and micro-impingers
86:
281:
Table 1 Major types of bioaerosol samplers (adapted from).
93:, as well as the heating, ventilation and air-conditioning (
258:
American Conference of Governmental Industrial Hygienists
376:
Concentration levels in different geographical regions
254:
National Institute for Occupational Safety and Health
541:
41 bars, 41 internet cafes, 44 classrooms, 20 homes
129:Factors influencing indoor bioaerosol generation
1051:
1049:
1047:
990:
988:
978:
976:
974:
1065:
1063:
1061:
1006:
1004:
1002:
1000:
137:, characteristics of air ventilation systems,
1037:
1035:
906:
904:
418:Viable bacteria: 970; Culturable fungi: 1200.
8:
858:
856:
854:
852:
875:
873:
871:
869:
812:
810:
842:
840:
838:
828:
826:
824:
822:
333:Glass fiber; Teflon filters; Polycarbonate
224:The ability of bioaerosols to cause human
782:
780:
778:
347:Identification and quantification methods
636:
556:Approaches to control indoor bioaerosols
544:Total bacteria and total fungi: 10–1000.
382:
279:
273:To enable subsequent identification and
736:
734:
732:
722:
720:
718:
716:
714:
634:
632:
630:
628:
626:
624:
622:
620:
618:
616:
591:
500:Fungi: 42.05 (Standard deviation=69.60)
300:Andersen impactor; SAS; Burkard sampler
768:
766:
764:
762:
760:
750:
748:
746:
686:
684:
682:
680:
678:
676:
674:
664:
662:
660:
658:
656:
654:
652:
650:
648:
646:
704:
702:
700:
698:
696:
606:
604:
7:
535:The city of Daegu, Republic of Korea
398:Average Concentration Level (CFU/m)
303:Cultivation; Microscopic analysis
14:
205:. One example is the well-known
73:Sources for indoor environments
68:Sources and influencing factors
269:Bioaerosol sampling techniques
264:Sampling and detection methods
43:and cell fragments of fungi),
1:
292:Possible Subsequent Analysis
297:Impactors and Sieve Samplers
1139:
483:Staphylococcus epidermidis
453:100 large office buildings
228:depend not only on their
203:insufficiency of evidence
184:gastrointestinal illness
491:The city of Boston, USA
415:27 (noncomplaint homes)
256:(NIOSH)), 1000 CFUs/m (
166:and different forms of
581:Sick building syndrome
401:Major Microbes Present
364:
207:sick building syndrome
538:June 2003-August 2004
466:Upper Silesia, Poland
412:April–September, 1991
362:
164:Legionnaires' disease
1123:Indoor air pollution
409:Midwestern area, USA
395:Sampling/Survey Size
322:RCS; Aerojet cyclone
319:Centrifugal Samplers
230:chemical composition
153:Human health effects
119:wastewater treatment
1113:Airborne pollutants
459:Mesophilic bacteria
428:Taipei area, Taiwan
389:Geographical Region
385:
282:
234:respiratory systems
168:bacterial pneumonia
83:ventilation systems
576:Indoor air quality
383:
365:
280:
172:coccidioidomycosis
139:seasonal variation
553:
552:
494:May 1997-May 1998
340:
339:
289:Example of Device
238:respiratory tract
143:building material
17:Indoor bioaerosol
1130:
1118:Building biology
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1095:
1089:
1086:
1080:
1077:
1071:
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1042:
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1027:
1021:
1018:
1012:
1008:
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727:
724:
709:
706:
691:
688:
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641:
638:
611:
608:
599:
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510:Hong Kong, China
447:25 states of USA
386:
283:
49:microbial toxins
1138:
1137:
1133:
1132:
1131:
1129:
1128:
1127:
1103:
1102:
1101:
1096:
1092:
1087:
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1068:
1059:
1054:
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707:
694:
689:
672:
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644:
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614:
609:
602:
597:
593:
589:
567:
558:
479:Micrococcus spp
378:
349:
330:Filter Cassette
271:
266:
188:causative agent
155:
131:
75:
70:
27:of biological (
12:
11:
5:
1136:
1134:
1126:
1125:
1120:
1115:
1105:
1104:
1100:
1099:
1090:
1081:
1072:
1057:
1043:
1031:
1022:
1013:
996:
984:
970:
953:
940:
931:
922:
912:
900:
891:
882:
865:
848:
834:
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728:
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89:, house dust,
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66:
13:
10:
9:
6:
4:
3:
2:
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989:
985:
979:
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950:
949:air pollution
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923:
916:
913:
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849:
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839:
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91:organic waste
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61:microorganism
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962:inflammation
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523:Cladosporium
522:
513:About 1 week
482:
478:
472:70 dwellings
392:Study Period
379:
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341:
272:
250:
223:
200:
160:tuberculosis
156:
132:
106:
102:
76:
57:plant fibers
16:
15:
527:Penicillium
404:References
147:infectivity
79:bioaerosols
1107:Categories
1070:1557-1563.
1011:5857-5863.
920:2339-2345.
880:3233-3239.
863:3941-3961.
587:References
571:Bioaerosol
497:21 offices
246:alveolitis
123:composting
21:bioaerosol
516:2 offices
469:1996-1998
450:1994-1998
431:July 1996
308:Impingers
211:headaches
176:influenza
115:hospitals
29:microbial
25:particles
565:See also
242:rhinitis
135:humidity
121:plants,
111:textiles
33:bacteria
966:allergy
286:Sampler
226:disease
215:fatigue
180:measles
77:Indoor
45:viruses
964:, and
217:, and
196:asthma
182:, and
53:pollen
41:spores
370:et al
107:et al
103:et al
39:(and
37:fungi
219:rash
192:mold
99:dust
95:HVAC
87:pets
547:N/A
503:N/A
440:N/A
421:N/A
190:of
19:is
1109::
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525:;
481:;
248:.
213:,
178:,
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35:,
252:(
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