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48:
in 1882. Certain species of bacteria have a waxy lipid called mycolic acid, in their cell walls which allow them to be stained with Acid-Fast better than a Gram-Stain. The unique ability of mycobacteria to resist decolorization by acid-alcohol is why they are termed acid-fast. It
336:
927:
750:
77:) acts as the chemical mordant. Since the Kinyoun stain is a cold method (no heat applied), the concentration of carbol fuschin used is increased.
387:
827:
309:
345:
333:
451:
920:
553:
358:
Murray PR, Baron E, Pfaller M, Tenover F, Yolken, Eds. Manual of clinical microbiology. 7th ed. Washington, DC: ASM, 1999.
61:(Z-N stain), the Kinyoun method of staining does not require heating. In the Ziehl–Neelsen stain, heat acts as a physical
645:
157:
782:
961:
812:
686:
206:
Make smear on a slide with organisms: Clean slide, wax label slide, spread organism, air dry for 10 minutes, heat fix
913:
251:, will also stain organisms that are not able to maintain the carbol fuchsin after decolorizing with HCl, such as
832:
695:
376:
Ananthanarayan and
Panicker's Textbook of Microbiology, 9th Edition, Universities Press (India), 2013, page 353
291:
Geo F. B et al, Jawetz, Melnick and
Adelberg's Medical Microbiology, 25th Edition, Lange Medical,2004, page 182
272:
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58:
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Baron EJ, Finegold SM. Bailey and Scott's diagnostic microbiology. 8th ed. St. Louis: Mosby, 1990.
122:
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28:
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523:
240:
50:
40:
429:
893:
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535:
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The
Kinyoun method can be modified as a weak acid fast stain, which uses 0.5–1.0%
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Briggs, Chris (July 2018). "Part 3: Differential and
Structural Staining".
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16:
Procedure used to stain certain acid-fast species of bacteria
389:
Microbiology Lab Manual: Discovering Your Tiny
Neighbors
901:
53:), a decolorizer (acid-alcohol), and a counterstain (
247:. The weak acid fast stain, in addition to staining
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349:, 8 September 2008. Retrieved on 1 November 2014.
161:Kinyoun stain on organism Mycobacterium smegmatis
921:
445:
221:Dip slide into Methylene blue for 30 seconds.
209:Dip slide into Carbol Fuchsin for 20 minutes.
49:involves the application of a primary stain (
44:. It is a variation of a method developed by
8:
215:Dip slide into Acid-alcohol for 3–5 seconds.
81:Summary of acid-fast stain (Kinyoun stain)
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914:
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395:(Lab manual). p. 58. Archived from
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751:Jaswant Singh–Bhattacharji (JSB) stain
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405:
7:
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880:
227:Blot slide dry with bibulous paper.
828:Grocott's methenamine silver stain
14:
461:Microbial and histological stains
346:American Society for Microbiology
884:
315:from the original on 2022-01-14
38:species of the bacterial genus
303:"KINYOUN CARBOL FUCHSIN STAIN"
1:
900:. You can help Knowledge by
332:Hussey, A. M., Zayaitz, A.,
27:(cold method), developed by
334:"Acid-Fast Stain Protocols"
978:
879:
554:Periodic acid–Schiff stain
230:Observe under Microscope.
179:Slide with organism smear
91:
88:
85:
31:, is a procedure used to
696:Light Green SF yellowish
687:Masson's trichrome stain
646:Auramine–rhodamine stain
301:.dalynn (October 2014).
273:Auramine-rhodamine stain
166:Make an acid-fast stain
896:-related article is a
813:Schaeffer–Fulton stain
783:Gömöri trichrome stain
162:
788:Luxol fast blue stain
663:Auramine phenol stain
160:
833:Warthin–Starry stain
706:Phosphomolybdic acid
849:Tissue stainability
621:Ziehl–Neelsen stain
481:Perls Prussian blue
82:
59:Ziehl–Neelsen stain
962:Microbiology stubs
823:Bielschowsky stain
725:Van Gieson's stain
691:Lillie's trichrome
339:2011-10-01 at the
163:
153:Staining procedure
80:
909:
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874:
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675:Connective tissue
245:hydrochloric acid
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29:Joseph J. Kinyoun
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701:Biebrich scarlet
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683:trichrome stain
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341:Wayback Machine
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260:Cryptosporidium
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108:Carbol fuchsin
86:Application of
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840:Wright's stain
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711:Fast Green FCF
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639:Methylene blue
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630:Carbol fuchsin
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604:Gentian violet
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402:on 2022-01-14.
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192:bibulous paper
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188:Methylene blue
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182:Carbol Fuchsin
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139:Methylene blue
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135:Counter Stain
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100:Non-acid fast
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75:carbol fuchsin
57:). Unlike the
55:methylene blue
21:Kinyoun method
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803:Movat's stain
801:
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798:Moeller stain
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773:Janus Green B
771:
767:
764:
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756:H&E stain
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741:Cresyl violet
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625:Kinyoun stain
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600:Methyl violet
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536:Carbohydrates
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524:Sudan Black B
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241:sulfuric acid
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51:basic fuchsin
47:
43:
42:
41:Mycobacterium
37:
34:
30:
26:
25:Kinyoun stain
22:
947:Bacteriology
902:expanding it
894:microbiology
891:
876:
866:Chromophobic
818:Silver stain
778:Giemsa stain
761:Haematoxylin
624:
397:the original
388:
381:
372:
363:
354:
344:
328:
317:. Retrieved
296:
287:
258:
257:species and
252:
249:Mycobacteria
248:
238:
235:Modification
201:Instructions
185:Acid-Alcohol
169:
123:Acid alcohol
119:Decolorizer
105:Primary dye
92:Cell colour
39:
24:
20:
18:
856:Acidophilic
808:Neutral red
793:Methyl blue
656:Rhodamine B
549:Mucicarmine
544:Alcian blue
499:Sudan stain
473:hemosiderin
416:|work=
243:instead of
224:Rinse Slide
218:Rinse Slide
212:Rinse slide
46:Robert Koch
957:Microscopy
941:Categories
861:Basophilic
716:Sirius Red
651:Auramine O
595:Gram stain
577:Thioflavin
319:2019-05-22
279:References
196:Microscope
130:Colorless
97:Acid fast
616:Acid-fast
572:Congo red
519:Oil Red O
509:Sudan III
418:ignored (
408:cite book
174:Materials
36:acid-fast
952:Staining
634:Fuchsine
609:Safranin
587:Bacteria
514:Sudan IV
504:Sudan II
337:Archived
310:Archived
267:See also
254:Nocardia
170:Source:
89:Reagent
766:Eosin Y
746:Cyanine
564:Amyloid
63:mordant
491:Lipids
71:carbol
67:phenol
65:while
892:This
734:Other
400:(PDF)
393:(PDF)
313:(PDF)
306:(PDF)
146:Blue
33:stain
898:stub
469:Iron
420:help
143:Red
127:Red
114:Red
111:Red
19:The
73:of
23:or
943::
685::
412::
410:}}
406:{{
343:,
308:.
263:.
929:e
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915:v
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689:/
632:/
623:/
602:/
471:/
453:e
446:t
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322:.
69:(
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
