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PANO1

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699:, isoform 1, located in the PONAB and PANTR species. These isoforms have proteins with 215 and 216 amino acids, respectively. No isoforms for the human PANO1 protein could be identified. Human PANO1 protein has a molecular weight of 22.8 kb and a theoretical, isoelectric point of 12.21. From an analysis of the PANO1 protein, it was observed that the protein contains a low amount of lysine and a very low amount of asparagine when compared to other human proteins. The same analysis indicated that the protein does not contain any hydrophobic or transmembrane regions. PANO1 contains 2 cAMP phosphorylation sites, 6 N- 819: 92: 117: 688: 1669: 716: 991: 123: 712:(SLC25A22). A causal link between this solute carrier, when upregulated, has been strongly associated with an osteosarcoma's ability to proliferate. In addition to promoting proliferation, SLC25A22 when expressed in vitro, progressed the cell cycle and inhibited apoptosis. It is curious that PANO1, an apoptosis inducer, and SLC25A22, an apoptosis inhibiter, overlap one another. 1958: 661:
is a protein in which in humans is modulated by the PANO1 gene. P14ARF is known to function as a tumor suppressor. When PANO1 is highly expressed in the cells, it is able to modulate p14ARF by stabilizing it and protecting it from degradation. With a confidence level of 5 out of 5, PANO1 has been
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is a protein that is a known tumor suppressor. It does this by controlling cell proliferation and cell survival, however the mechanism for how this process is controlled/modulated remained unclear. PANO1 has been identified to modulate and stabilize p14ARF by stabilizing it and protecting it from
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of the cell. PANO1 is an intron-less gene. Intron-less genes only make up about 3% of the human genome. A functional analysis of these types of genes revealed that they often have tissue-specific expression in tissues such as the nervous system and testis. This kind of expression is commonly
1684:. The date of divergence as well as amino acid changes were tracked over many different species types to generate a divergence date vs. number of amino acids changes as seen to the right. PANO1 appears to diverge much more quickly than fibrinogen alpha and much more quickly than cytochrome c. 703:
sites, 4 protein kinase C phosphorylation sites, 3 bipartite nuclear localization signals as well as arginine-rich and proline-rich regions. Using PSORTII, 3 discrimination of nuclear localization signals were identified. Pat4 (RRRR) at position 200, Pat7 (RRRR) at position 201 and a bipartite
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One promoter region was identified using Genomatix. It is located on the positive strand and is 1040 bp in length. Its start site is located at 795633 and it ends at 796672. It overlaps with the primary transcript which starts at 796633. 440
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PANO1 orthologs were only able to be traced back in divergence to birds. Much more closely related orthologs include primates, as well as marsupial and placental mammals. Specific examples of orthologs can be seen in the table below.
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associated with neuropathies, disease, and cancer. The tissue types that PANO1 has the highest expression in, are the cerebellum regions of the brain as well as pituitary and testis tissues.
762:, spermatogenic zip 1 transcription factor, signal transducer and activator of transcription, pleomorphic adenoma gene, general transcription factor IIIA, stimulating protein 1, 2124: 130: 1672:
Date of divergence in millions of years vs. "m" (changes / 100 amino acids) graph comparing PANO1 to two different proteins, fibrinogen alpha as well as cytochrome c.
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Watari A, Li Y, Higashiyama S, Yutsudo M (February 2012). "A novel proapoptotic gene PANO encodes a post-translational modulator of the tumor suppressor p14ARF".
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Grzybowska EA (July 2012). "Human intronless genes: functional groups, associated diseases, evolution, and mRNA processing in absence of splicing".
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A possible cleavage site was identified between amino acids 33 and 34 as depicted in the PANO1 protein model. As mentioned previously, 6 N-
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cell line with a rank of 97.  PANO1 is biased to being expressed in androgen sensitive cells compared to androgen insensitive cells.
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PANO1 is also known as Proapoptotic Nucleolar Protein 1, PANO, and Pre-mRNA-splicing Factor CW22-like. PANO1 is located on human
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Predicted secondary structure of PANO1 protein generated from Phyre2 and annotated to show alpha helices as well as a beta strand
1744: 763: 116: 91: 759: 1740: 705: 129: 679:
at positions 797,511-799,190 and is positioned on the + strand. Its protein contains 1 exon and 215 amino acids.
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Predicted 3' UTR DNA folding structure generated from Unafold program and annotated to be able to see stem loops.
1717: 818: 1867: 1625: 1548: 1354: 1199: 708:
allow proteins to be able to enter the nucleus, but many nuclear proteins possess their own. PANO1 overlaps
423: 1677: 1316: 1277: 654: 105: 2043:"SLC25A22 Promotes Proliferation and Metastasis of Osteosarcoma Cells via the PTEN Signaling Pathway" 758:
were identified within the promoter region. To highlight some of relevance and importance to PANO1:
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sites, 2 cAMP phosphorylation sites, and 4 protein kinase C phosphorylation sites are also present.
553: 508: 1587: 1432: 1238: 729: 65: 687: 1668: 715: 2072: 1913: 1844: 37: 2062: 2054: 1905: 1836: 1791:"PANO1 - Proapoptotic nucleolar protein 1 - Homo sapiens (Human) - PANO1 gene & protein" 1790: 1160: 990: 209: 140: 61: 783: 696: 184: 2193: 2145: 2067: 2042: 1393: 1121: 920: 908: 896: 884: 872: 860: 839: 700: 2169: 1700:. When PANO1 is over-expressed, as a direct result, p14ARF expression also increases. 806:
cell lines. It also appears that PANO1 is highly expressed in the androgen-sensitive
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meaning the protein is able to move around easily. The secondary structure reveals a
2216: 795: 676: 1681: 1471: 1082: 1043: 202: 82: 69: 1840: 1762:
National Center for Biotechnology Information, U.S. National Library of Medicine
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A predicted 3' UTR structure was generated using Unafold and depicts predicted
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PANO1 protein model with motifs generated through IBS tool at Cuckoo Workgroup
99: 2058: 1985: 1716:. Additionally, other diseases that have been associated with PANO1 include 827: 663: 650: 344: 302: 223: 168: 155: 57: 2076: 1917: 1848: 632: 1934: 787: 778:
Like previously mentioned, PANO1 is detected to be most expressed in the
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is a protein which in humans is encoded by the PANO1 gene. PANO1 is an
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Phylogenic tree of PANO1 generated using date of divergence data.
1697: 791: 2194:"Protein BLAST: search protein databases using a protein query" 2170:"DNA folding results on 21Aug01-20-42-28 for 73.242.41.233" 1959:"GeneLoc Integrated Map for Chromosome 11: Search Results" 653:
inducing protein that is able to regulate the function of
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Geo profiles indicated that PANO1 is highly expressed in
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structures. Two stem loop structures are zoomed in on.
192: 1712:, scientists have seen a decrease in tumorigenicity in 314: 567: 546: 522: 501: 1736: 1734: 1732: 1708:When there is an abnormal expression of PANO1 in 139: 2036: 2034: 1891: 1889: 1887: 1822: 1820: 1818: 1816: 1814: 1812: 1810: 1986:"SAPS < Sequence Statistics < EMBL-EBI" 8: 1741:GRCh38: Ensembl release 89: ENSG00000288675 770:elements and HMG box-containing protein 1. 340: 180: 77: 2066: 1001: 844: 48:, PANO, proapoptotic nucleolar protein 1 1728: 1676:PANO1 was compared to two other genes, 16:Mammalian protein found in Homo sapiens 971:protein kinase C phosphorylation site 963:protein kinase C phosphorylation site 955:protein kinase C phosphorylation site 947:protein kinase C phosphorylation site 18: 2140: 2138: 2119: 2117: 2115: 2113: 2088: 2086: 2009: 2007: 2005: 704:(RKGTPTARCLGQRTKEK) at position 35. 144: 103: 98: 7: 1980: 1978: 1929: 1927: 1862: 1860: 1858: 1785: 1783: 1781: 1779: 1777: 1775: 1773: 1771: 1028:Seq. identity to HSA prot. (#/100) 2094:"Phyre 2 Results for PANO1_phyre2" 710:solute carrier family 25 member 22 564: 543: 519: 498: 476: 457: 433: 414: 390: 371: 319: 280: 218: 197: 14: 1031:Seq. similarity to HSA prot. (%) 981:Homology and evolutionary history 662:theorized to be expressed in the 128: 121: 115: 90: 2041:Chen MW, Wu XJ (January 2018). 1025:Seq. identity to HSA prot. (%) 764:CCAAT/enhancer binding protein 728:The structure of PANO1 is 82% 303:More reference expression data 238:right hemisphere of cerebellum 1: 2146:"PANO1 - GEO Profiles - NCBI" 986:Orthologs and phylogenic tree 113: 2223:Genes on human chromosome 11 1244:white-tufted-eared marmoset 736:at positions 3-9 as well as 706:Nuclear localization signals 1841:10.1016/j.yexcr.2011.10.019 814:Transcript level regulation 242:stromal cell of endometrium 2239: 2125:"Genomatix Software Suite" 1910:10.1016/j.bbrc.2012.06.092 1898:Biochem Biophys Res Commun 939:cAMP phosphorylation site 931:cAMP phosphorylation site 1758:"Human PubMed Reference:" 1016:Date of divergence (MYA) 740:incorporated throughout. 636: 631: 627: 620: 601: 584: 571: 550: 539: 526: 505: 494: 483: 479: 464: 460: 451: 440: 436: 421: 417: 408: 397: 393: 378: 374: 365: 350: 343: 339: 327: 322: 313: 300: 287: 283: 230: 221: 191: 183: 179: 162: 149: 112: 89: 80: 76: 55: 52: 42: 35: 30: 26: 21: 2059:10.1177/1533033818811143 2047:Technol Cancer Res Treat 1963:genecards.weizmann.ac.il 846:Protein motif locations 834:Protein level regulation 683:Transcripts and proteins 1626:Melopsittacus undulatus 1549:Ursus arctos horribilis 1355:Leptonychotes weddellii 1200:Rhinopithecus roxellana 790:tissues. Furthermore, 2198:blast.ncbi.nlm.nih.gov 1678:fibrinogen alpha chain 1673: 1438:African bush elephant 1317:Phascolarctos cinereus 995: 823: 760:Wilms tumor suppressor 720: 692: 270:superior frontal gyrus 2015:"PSORT II Prediction" 1704:Clinical significance 1671: 1278:Propithecus coquereli 993: 821: 756:transcription factors 744:Gene level regulation 718: 690: 657:. More specifically, 266:primary visual cortex 106:Chromosome 11 (human) 2150:www.ncbi.nlm.nih.gov 2098:www.sbg.bio.ic.ac.uk 2053:: 1533033818811143. 1688:Interacting proteins 1007:Genus & species 590:Chr 11: 0.8 – 0.8 Mb 1935:"Human BLAT Search" 1088:Sumatran orangutan 1003: 847: 258:right lobe of liver 1674: 1588:Oxyura jamaicensis 1433:Loxodonta africana 1283:Coquerel's sifaka 1239:Callithrix jacchus 1002: 996: 845: 824: 721: 693: 254:anterior pituitary 1872:www.genecards.org 1718:hemochromatosis 2 1661: 1660: 1022:Seq. length (aa) 978: 977: 644: 643: 640: 639: 616: 615: 580: 579: 561: 560: 535: 534: 516: 515: 490: 489: 473: 472: 447: 446: 430: 429: 404: 403: 387: 386: 335: 334: 331: 330: 309: 308: 296: 295: 277: 276: 175: 174: 70:PANO1 - orthologs 2230: 2208: 2207: 2205: 2204: 2190: 2184: 2183: 2181: 2180: 2166: 2160: 2159: 2157: 2156: 2142: 2133: 2132: 2121: 2108: 2107: 2105: 2104: 2090: 2081: 2080: 2070: 2038: 2029: 2028: 2026: 2025: 2011: 2000: 1999: 1997: 1996: 1982: 1973: 1972: 1970: 1969: 1955: 1949: 1948: 1946: 1945: 1931: 1922: 1921: 1893: 1882: 1881: 1879: 1878: 1864: 1853: 1852: 1824: 1805: 1804: 1802: 1801: 1787: 1766: 1765: 1754: 1748: 1738: 1161:Hylobates moloch 1127:tufted capuchin 1004: 848: 655:tumor suppressor 629: 628: 612: 597: 592: 575: 565: 556: 544: 540:RefSeq (protein) 530: 520: 511: 499: 477: 458: 434: 415: 391: 372: 341: 320: 305: 281: 250:body of pancreas 226: 224:Top expressed in 219: 198: 181: 171: 158: 147: 132: 125: 119: 108: 94: 78: 72: 47: 40: 19: 2238: 2237: 2233: 2232: 2231: 2229: 2228: 2227: 2213: 2212: 2211: 2202: 2200: 2192: 2191: 2187: 2178: 2176: 2174:www.unafold.org 2168: 2167: 2163: 2154: 2152: 2144: 2143: 2136: 2123: 2122: 2111: 2102: 2100: 2092: 2091: 2084: 2040: 2039: 2032: 2023: 2021: 2013: 2012: 2003: 1994: 1992: 1984: 1983: 1976: 1967: 1965: 1957: 1956: 1952: 1943: 1941: 1939:genome.ucsc.edu 1933: 1932: 1925: 1895: 1894: 1885: 1876: 1874: 1866: 1865: 1856: 1826: 1825: 1808: 1799: 1797: 1795:www.uniprot.org 1789: 1788: 1769: 1756: 1755: 1751: 1739: 1730: 1726: 1706: 1696:degradation in 1690: 1666: 1640:XP_033919672.1 1602:XP_035173110.1 1563:XP_026346196.1 1525:XP_023096045.1 1486:XP_012391505.1 1447:XP_010600675.1 1408:XP_032270736.1 1369:XP_030893169.1 1331:XP_020836186.1 1292:XP_012507388.1 1253:XP_009007032.2 1214:XP_030773457.1 1175:XP_032005218.1 1166:silvery gibbon 1136:XP_024111240.1 1097:XP_024111240.1 1058:XP_034607700.1 988: 983: 836: 816: 776: 751: 746: 726: 685: 673: 633:View/Edit Human 610: 595: 588: 585:Location (UCSC) 573: 552: 528: 507: 424:ENSG00000288675 301: 292: 273: 268: 264: 260: 256: 252: 248: 246:pituitary gland 244: 240: 236: 222: 166: 153: 145: 135: 134: 133: 126: 104: 81:Gene location ( 56: 43: 36: 17: 12: 11: 5: 2236: 2234: 2226: 2225: 2215: 2214: 2210: 2209: 2185: 2161: 2134: 2109: 2082: 2030: 2001: 1974: 1950: 1923: 1883: 1854: 1806: 1767: 1749: 1727: 1725: 1722: 1705: 1702: 1689: 1686: 1665: 1662: 1659: 1658: 1655: 1652: 1650: 1647: 1644: 1641: 1638: 1635: 1632: 1629: 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1861: 1859: 1855: 1850: 1846: 1842: 1838: 1835:(3): 187–95. 1834: 1830: 1823: 1821: 1819: 1817: 1815: 1813: 1811: 1807: 1796: 1792: 1786: 1784: 1782: 1780: 1778: 1776: 1774: 1772: 1768: 1763: 1759: 1753: 1750: 1746: 1742: 1737: 1735: 1733: 1729: 1723: 1721: 1719: 1715: 1711: 1703: 1701: 1699: 1694: 1687: 1685: 1683: 1679: 1670: 1663: 1656: 1653: 1651: 1648: 1645: 1642: 1639: 1636: 1633: 1630: 1628: 1627: 1623: 1622: 1618: 1615: 1613: 1610: 1607: 1604: 1601: 1598: 1595: 1592: 1590: 1589: 1585: 1584: 1580: 1577: 1574: 1571: 1568: 1565: 1562: 1559: 1556: 1554:grizzly bear 1553: 1551: 1550: 1546: 1545: 1541: 1538: 1536: 1533: 1530: 1527: 1524: 1521: 1518: 1515: 1513: 1512: 1508: 1507: 1503: 1500: 1497: 1494: 1491: 1488: 1485: 1482: 1479: 1477:killer whale 1476: 1474: 1473: 1469: 1468: 1464: 1461: 1458: 1455: 1452: 1449: 1446: 1443: 1440: 1437: 1435: 1434: 1430: 1429: 1425: 1422: 1419: 1416: 1413: 1410: 1407: 1404: 1401: 1398: 1396: 1395: 1391: 1390: 1386: 1383: 1380: 1377: 1374: 1371: 1368: 1365: 1362: 1360:Weddell seal 1359: 1357: 1356: 1352: 1351: 1347: 1344: 1342: 1339: 1336: 1333: 1330: 1327: 1324: 1321: 1319: 1318: 1314: 1313: 1309: 1306: 1303: 1300: 1297: 1294: 1291: 1288: 1285: 1282: 1280: 1279: 1275: 1274: 1270: 1267: 1264: 1261: 1258: 1255: 1252: 1249: 1246: 1243: 1241: 1240: 1236: 1235: 1231: 1228: 1225: 1222: 1219: 1216: 1213: 1210: 1207: 1204: 1202: 1201: 1197: 1196: 1192: 1189: 1186: 1183: 1180: 1177: 1174: 1171: 1168: 1165: 1163: 1162: 1158: 1157: 1153: 1150: 1147: 1144: 1141: 1138: 1135: 1132: 1129: 1126: 1124: 1123: 1119: 1118: 1114: 1111: 1108: 1105: 1102: 1099: 1096: 1093: 1090: 1087: 1085: 1084: 1080: 1079: 1075: 1072: 1069: 1066: 1063: 1060: 1057: 1054: 1051: 1048: 1046: 1045: 1041: 1040: 1036: 1033: 1030: 1027: 1024: 1021: 1018: 1015: 1012: 1009: 1006: 1005: 1000: 992: 985: 980: 973: 970: 969: 965: 962: 961: 957: 954: 953: 949: 946: 945: 941: 938: 937: 933: 930: 929: 925: 922: 918: 917: 913: 910: 906: 905: 901: 898: 894: 893: 889: 886: 882: 881: 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88: 84: 79: 75: 71: 67: 63: 59: 51: 46: 39: 34: 29: 25: 20: 2201:. Retrieved 2197: 2188: 2177:. Retrieved 2173: 2164: 2153:. Retrieved 2149: 2128: 2101:. Retrieved 2097: 2050: 2046: 2022:. Retrieved 2019:psort.hgc.jp 2018: 1993:. Retrieved 1989: 1966:. Retrieved 1962: 1953: 1942:. Retrieved 1938: 1901: 1897: 1875:. Retrieved 1871: 1832: 1829:Exp Cell Res 1828: 1798:. Retrieved 1794: 1761: 1752: 1707: 1691: 1682:cytochrome C 1675: 1657:124.7228313 1624: 1586: 1581:50.51749523 1547: 1542:51.31616836 1509: 1504:88.64892406 1472:Orcinus orca 1470: 1465:94.16085399 1431: 1426:60.60524737 1399:harbor seal 1392: 1387:73.64723053 1353: 1348:125.5266099 1315: 1310:36.70139217 1276: 1271:32.15836241 1237: 1232:14.35240101 1198: 1193:4.677719156 1159: 1154:3.770186718 1120: 1115:3.770186718 1083:Pongo abelii 1081: 1044:Homo sapiens 1042: 1019:Accession # 1010:Common name 997: 837: 825: 800:liposarcomas 777: 752: 727: 694: 674: 646: 645: 572: 554:NP_001280096 551: 527: 509:NM_001293167 506: 484: 465: 441: 422: 398: 379: 359: 354: 208: 201: 53:External IDs 1680:as well as 1631:budgerigar 1619:122.894879 1593:ruddy duck 1511:Felis catus 1013:Tax. group 734:beta strand 262:granulocyte 31:Identifiers 2203:2021-08-01 2179:2021-08-01 2155:2021-08-01 2103:2021-08-01 2024:2021-08-01 1995:2021-08-01 1968:2021-08-01 1944:2021-08-01 1904:(1): 1–6. 1877:2021-07-30 1800:2021-08-01 1747:, May 2017 1724:References 1710:HeLa cells 1698:HeLa cells 1664:Divergence 1557:placental 1519:placental 1516:house cat 1480:placental 1441:placental 1402:placental 1363:placental 1325:marsupial 802:, and the 780:cerebellum 774:Expression 730:disordered 212:(ortholog) 2129:Genomatix 1714:nude mice 828:stem loop 784:pituitary 724:Structure 664:nucleolus 651:apoptosis 381:101927423 345:Orthologs 58:GeneCards 2217:Category 2077:30482097 1918:22732409 1849:22094112 1743:– 1286:primate 1247:primate 1208:primate 1169:primate 1130:primate 1091:primate 974:191-193 942:136-139 926:186-191 914:120-125 902:111-116 749:Promoter 622:Wikidata 234:testicle 167:799,190 154:797,511 2068:6259056 1868:"PANO1" 1745:Ensembl 1654:0.7127 1649:0.2873 1616:0.7074 1611:0.2926 1578:0.3966 1572:0.6034 1539:0.4014 1534:0.5986 1501:0.5879 1495:0.4121 1423:0.4545 1417:0.5455 1384:0.5212 1378:0.4788 1307:0.3072 1301:0.6928 1229:0.1337 1223:0.8663 1190:0.0457 1184:0.9543 697:isoform 453:UniProt 410:Ensembl 351:Species 187:pattern 146:11p15.5 38:Aliases 2075:  2065:  1916:  1847:  1693:p14ARF 1646:28.73 1608:29.26 1569:60.34 1531:59.86 1492:41.21 1414:54.55 1375:47.88 1345:0.715 1340:0.285 1322:koala 1298:69.28 1268:0.275 1262:0.725 1220:86.63 1211:28.21 1181:95.43 1151:0.037 1145:0.963 1112:0.037 1106:0.963 1094:15.76 1052:human 1049:Human 966:57-59 958:40-42 950:34-36 934:35-38 890:71-76 878:33-38 866:23-28 851:Motif 808:LAPC-4 804:testis 788:testis 768:GC box 659:P14ARF 605:search 603:PubMed 467:I0J062 367:Entrez 315:BioGPS 1634:bird 1596:bird 1462:0.61 1456:0.39 1337:28.5 1289:74.1 1259:72.5 1250:42.9 1172:19.8 1142:96.3 1133:43.2 1103:96.3 923:site 911:site 899:site 887:site 875:site 863:site 647:PANO1 360:Mouse 355:Human 210:Mouse 203:Human 150:Start 83:Human 62:PANO1 45:PANO1 22:PANO1 2073:PMID 1914:PMID 1845:PMID 1643:228 1637:312 1605:278 1599:312 1566:290 1528:217 1489:237 1450:204 1444:105 1411:264 1372:182 1334:205 1328:160 1295:183 1256:268 1217:239 1178:175 1148:100 1139:216 1109:100 1100:216 1070:100 1064:100 1061:215 792:NCBI 786:and 671:Gene 193:Bgee 141:Band 100:Chr. 2063:PMC 2055:doi 1906:doi 1902:424 1837:doi 1833:318 1575:53 1560:96 1522:96 1498:91 1483:96 1459:85 1453:39 1420:67 1405:96 1381:70 1366:96 1304:71 1265:91 1226:86 1187:81 611:n/a 596:n/a 574:n/a 529:n/a 485:n/a 442:n/a 399:n/a 323:n/a 290:n/a 163:End 66:OMA 2219:: 2196:. 2172:. 2148:. 2137:^ 2127:. 2112:^ 2096:. 2085:^ 2071:. 2061:. 2051:17 2049:. 2045:. 2033:^ 2017:. 2004:^ 1988:. 1977:^ 1961:. 1937:. 1926:^ 1912:. 1900:. 1886:^ 1870:. 1857:^ 1843:. 1831:. 1809:^ 1793:. 1770:^ 1760:. 1731:^ 1720:. 1076:0 1073:0 1067:1 1055:0 1037:m 1034:n 919:N- 907:N- 895:N- 883:N- 871:N- 859:N- 798:, 782:, 766:, 169:bp 156:bp 64:; 60:: 2206:. 2182:. 2158:. 2131:. 2106:. 2079:. 2057:: 2027:. 1998:. 1971:. 1947:. 1920:. 1908:: 1880:. 1851:. 1839:: 1803:. 1764:. 85:) 68::

Index

Aliases
PANO1
GeneCards
PANO1
OMA
PANO1 - orthologs
Human
Chromosome 11 (human)
Chr.
Chromosome 11 (human)
Chromosome 11 (human)
Genomic location for PANO1
Genomic location for PANO1
Band
bp
bp
RNA expression
Bgee
Human
Mouse
Top expressed in
More reference expression data
BioGPS
Orthologs
Entrez
101927423
Ensembl
ENSG00000288675
UniProt
I0J062

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