65:
2587:
Hamilton SR, Davidson RC, Sethuraman N, Nett JH, Jiang Y, Rios S, Bobrowicz P, Stadheim TA, Li H, Choi BK, Hopkins D, Wischnewski H, Roser J, Mitchell T, Strawbridge RR, Hoopes J, Wildt S, Gerngross TU (September 2006). "Humanization of yeast to produce complex terminally sialylated glycoproteins".
560:
was mainly used as the host system for transformation. Due to its abilities of recombination with foreign DNA and processing large proteins, much research has been carried out to investigate the possibility of producing new proteins and the function of artificially designed proteins, using
202:. Later studies have further distinguished new species in this genus, resulting in a total of 7 recognized species. It is not uncommon to see the old name still in use in the context of protein production, as of 2023; in less formal use, the yeast may confusingly be referred to as
873:
can grow up to a very high cell density. Under ideal conditions, it can multiply to the point where the cell suspension is practically a paste. As the protein yield from expression system in a microbe is roughly equal to the product of the proteins produced per cell, which makes
1602:
M. M. Guarna G. J. Lesnicki B. M. Tam J. Robinson C. Z. Radziminski D. Hasenwinkle A. Boraston E. Jervis R. T. A. MacGillivray R. F. B. Turner D. G. Kilburn (1997). "On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris".
1144:
Yamada, Yuzo; Matsuda, Minako; Maeda, Kojiro; Mikata, Kozaburo (January 1995). "The
Phylogenetic Relationships of Methanol-assimilating Yeasts Based on the Partial Sequences of 18S and 26S Ribosomal RNAs: The Proposal of Komagataella Gen. Nov. (Saccharomycetaceae)".
747:
is unable to produce a number of proteins, since it does not contain the appropriate chaperones. The technologies of introducing genes of mammalian chaperonins into the yeast genome and overexpressing existing chaperonins still require improvement.
1681:
Brigitte Gasser, Roland
Prielhofer, Hans Marx, Michael Maurer, Justyna Nocon, Matthias Steiger, Verena Puxbaum, Michael Sauer & Diethard Mattanovich (2013). "Pichia pastoris: protein production host and model organism for biomedical research".
975:
in its normal glycosylation form, by exchanging the enzymes responsible for the fungal type glycosylation, with the mammalian homologs. Thus, the altered glycosylation pattern allowed the protein to be fully functional.
1859:
Gould, S. J., McCollum, D., Spong, A. P., Heyman, J. A., & Subramani, S. (1992). "Development of the yeast Pichia pastoris as a model organism for a genetic and molecular analysis of peroxisome assembly".
428:
can be grown and used easily in lab. Like other widely used yeast models, it has relatively short life span and fast regeneration time. Moreover, some inexpensive culture media have been designed, so that
2250:
Morton, C. L., & Potter, P. M. (2000). "Comparison of
Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Spodoptera frugiperda, and COS7 cells for recombinant gene expression".
2199:
Zhou, X., Yu, Y., Tao, J., & Yu, L. (2014). "Production of LYZL6, a novel human c-type lysozyme, in recombinant Pichia pastoris employing high cell density fed-batch fermentation".
692:
promoter, which means that protein production can be induced by the addition of methanol on medium. After several researches, scientists found that the promotor derived from
2360:"Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects"
2674:
2295:
2236:
2072:
1968:
1903:
1845:
1786:
1719:
1588:
1513:
1409:
1101:
2972:
2828:
2088:"Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A"
2998:
2553:"Increased expression and secretion of recombinant hIFNγ through amino acid starvation-induced selective pressure on the adjacent HIS4 gene in Pichia pastoris"
715:
can grow with extremely high cell density on the culture. This feature is compatible with heterologous protein expression, giving higher yields of production.
44:
603:
are used for biotechnical purposes, with significant differences among them in growth and protein production. Some common variants possess a mutation in the
867:
with reasonably strong methanol solution, which would kill most other micro-organisms. In this case, the expression system is cheap to set up and maintain.
2639:
Spohner, S. C., Müller, H., Quitmann, H., & Czermak, P. (2015). "Expression of enzymes for the usage in food and feed industry with Pichia pastoris".
409:. The growth rate of its colonies can vary by a large range, from near to 0 to a doubling time of one hour, which is suitable for industrial processes.
1735:"Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor"
514:
has the ability to functionally process proteins with large molecular weight, which is useful in a translational host. Considering all the advantages,
537:, with complete genome data and its ability to carry out complex eukaryotic genetic processing in a relatively small genome. The functional genes for
2127:
Daly R, Hearn MT (2005). "Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production".
2959:
2815:
2201:
449:
system. The complete genomic data allows scientists to identify homologous proteins and evolutionary relationships between other yeast species and
1354:
2480:
2439:
1117:
770:, due to its features of fast growth rate, high protein production rate, as well as undemanding growth conditions. Protein production in
726:, which is one of the most well-studied yeast model organisms. As a result, the experiment protocol and materials are easy to build for
1618:
465:. Then the homologous comparison to other more complicated eukaryotic species can be processed, to obtain their functions and origins.
825:
might produce a misfolded protein when disulfides are included in final product, leading to inactive or insoluble forms of proteins.
2048:
1821:
1444:
1255:"Biotechnological strains of Komagataella (Pichia) pastoris are Komagataella phaffii as determined from multigene sequence analysis"
2087:
797:, so that a large number of clones has to be screened for protein production, to find the best producer. The biggest advantage of
2172:
2092:
1605:
1529:"Improvement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris"
3064:
2422:
Cregg JM, Tolstorukov I, Kusari A, Sunga J, Madden K, Chappell T (2009). "Chapter 13 Expression in the Yeast Pichia pastoris".
2552:
1923:
2105:
1640:
De
Schutter K, Lin YC, Tiels P, Van Hecke A, Glinka S, Weber-Lehmann J, Rouzé P, Van de Peer Y, Callewaert N (June 2009).
1297:
Heistinger, L; Dohm, JC; Paes, BG; Koizar, D; Troyer, C; Ata, Ö; Steininger-Mairinger, T; Mattanovich, D (25 April 2022).
666:
486:
have been well studied for decades and used by researchers for various purposes throughout history. The two yeast genera;
2311:"Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris"
64:
3049:
2691:
Barone, GD; Emmerstorfer-Augustin, A; Biundo, A; Pisano, I; Coccetti, P; Mapelli, V; Camattari, A (26 February 2023).
1350:"Pichia pastoris exhibits high viability and a low maintenance energy requirement at near-zero specific growth rates"
3003:
1000:
can keep the bread soft. Meanwhile, in beer, enzymes could be used to lower the alcohol concentration. Recombinant
889:
612:
441:
GS115 genome has been sequenced by the
Flanders Institute for Biotechnology and Ghent University, and published in
1460:
Zörgö E, Chwialkowska K, Gjuvsland AB, Garré E, Sunnerhagen P, Liti G, Blomberg A, Omholt SW, Warringer J (2013).
608:
305:
1348:
Rebnegger, C., Vos, T., Graf, A. B., Valli, M., Pronk, J. T., Daran-Lapujade, P., & Mattanovich, D. (2016).
1299:"Genotypic and phenotypic diversity among Komagataella species reveals a hidden pathway for xylose utilization"
604:
474:
264:
1206:"Microbe Profile: Komagataella phaffii: a methanol devouring biotech yeast formerly known as Pichia pastoris"
254:
2252:
936:
884:
1984:"Comparative genome-scale analysis of Pichia pastoris variants informs selection of an optimal base strain"
274:
3054:
2744:
2506:"Is Pichia pastoris a realistic platform for industrial production of recombinant human interferon gamma?"
912:. These types of media significantly increase the cost of producing heterologous proteins. Additionally,
897:
573:
platforms, which is a typical application for a standard experimental model organism, as described below.
284:
295:
2668:
2289:
2230:
1962:
1897:
1839:
1780:
1713:
1582:
1507:
1403:
1095:
398:
315:
2463:
Brondyk WH (2009). "Chapter 11 Selecting an
Appropriate Method for Expressing a Recombinant Protein".
700:
is extremely suitable to control the expression of foreign genes, which had been transformed into the
43:
3013:
2933:
2856:
2807:
2597:
2371:
1542:
1363:
1115:
Koichi Ogata, Hideo
Nishikawa & Masahiro Ohsugi (1969). "A Yeast Capable of Utilizing Methanol".
1064:
374:
2951:
1800:
Heidebrecht, Aniela, and Thomas
Scheibel (2013). "Recombinant production of spider silk proteins".
1684:
971:. In 2006, a research group managed to create a new strain called YSH597. This strain can express
640:
378:
401:, haploid cells undergo mitosis for reproduction. In the sexual life cycle, diploid cells undergo
2621:
2533:
2340:
2277:
2152:
2066:
1885:
669:
226:
59:
2170:
Romanos, Mike. (1995). "Advances in the use of Pichia pastoris for high-level gene expression".
424:
was investigated and identified as a good model organism with several advantages. First of all,
2911:
2833:
3021:
2977:
2920:
2864:
2776:
2724:
2656:
2613:
2525:
2486:
2476:
2445:
2435:
2399:
2332:
2269:
2218:
2144:
2109:
2054:
2044:
2013:
1950:
1877:
1827:
1817:
1768:
1701:
1663:
1622:
1570:
1495:
1440:
1391:
1330:
1276:
1235:
1227:
1162:
1083:
757:
570:
175:
116:
3026:
2869:
2714:
2704:
2648:
2605:
2567:
2517:
2468:
2427:
2389:
2379:
2322:
2261:
2210:
2181:
2136:
2101:
2036:
2003:
1995:
1940:
1932:
1869:
1809:
1758:
1748:
1693:
1653:
1614:
1560:
1550:
1533:
1485:
1475:
1432:
1381:
1371:
1320:
1310:
1266:
1217:
1154:
1126:
1073:
956:
878:
of great use when trying to produce large quantities of protein without expensive equipment.
762:
708:
534:
126:
2086:
Wenhui Zhang Mark A. Bevins
Bradley A. Plantz Leonard A. Smith Michael M. Meagher. (2000).
3059:
1001:
864:
654:
588:
446:
222:
106:
2719:
2692:
2601:
2375:
1546:
1367:
2946:
2802:
2394:
2359:
2008:
1983:
1813:
1763:
1734:
1565:
1528:
1490:
1461:
1436:
1386:
1349:
1325:
1298:
989:
972:
968:
952:
779:
479:
230:
2521:
2472:
2431:
1945:
1918:
3043:
2185:
1739:
1466:
993:
960:
909:
900:
from multicellular organisms require complex and expensive types of media, including
814:
498:
218:
2625:
2537:
2344:
2281:
859:, meaning that it can grow with the simple methanol, as the only source of energy —
766:
is the most frequently used organism for expression system, to produce heterologous
2781:
2156:
1889:
1130:
856:
688:. Usually, the gene for the desired protein is introduced under the control of the
592:
214:
168:
2925:
2652:
996:, with many functions. For example, some enzymes produced by genetically modified
2896:
2214:
1480:
346:. They are heterotrophs and they can use several carbon sources for living, like
2905:
1016:
921:
901:
2384:
1555:
1315:
959:. At the beginning, one drawback of this protein expression system is the over-
17:
2327:
2310:
2265:
2040:
2031:
Higgins, D. R., & Cregg, J. M. (1998). "Introduction to Pichia pastoris".
1753:
1271:
1254:
740:
538:
493:
458:
433:
can grow quickly on them, with high cell density. Whole genome sequencing for
96:
2767:
2693:"Industrial Production of Proteins with Pichia pastoris-Komagataella phaffii"
2504:
Razaghi A, Tan E, Lua LH, Owens L, Karthikeyan OP, Heimann K (January 2017).
1231:
2789:
2609:
2572:
1917:
Cregg, J. M., Barringer, K. J., Hessler, A. Y., & Madden, K. R. (1985).
1642:"Genome sequence of the recombinant protein production host Pichia pastoris"
1060:"Genome sequence of the recombinant protein production host Pichia pastoris"
1053:
1051:
810:
644:
518:
can be usefully employed as both a genetic and experimental model organism.
386:
76:
2728:
2660:
2617:
2529:
2490:
2449:
2403:
2358:
Yu, Xiao-Wei; Sun, Wei-Hong; Wang, Ying-Zheng; Xu, Yan (24 November 2017).
2336:
2273:
2222:
2148:
2113:
2017:
1831:
1772:
1705:
1667:
1626:
1574:
1499:
1395:
1334:
1280:
1239:
1087:
2058:
1954:
1936:
1881:
1873:
1619:
10.1002/(SICI)1097-0290(19971105)56:3<279::AID-BIT5>3.0.CO;2-G
1166:
2985:
2890:
2841:
2761:
2709:
1376:
1222:
1205:
917:
767:
681:
355:
351:
343:
185:
1204:
Heistinger, Lina; Gasser, Brigitte; Mattanovich, Diethard (2020-07-01).
939:. All the applications are based on its feature of expressing proteins.
541:
assembly were investigated by comparing wild-type and mutant strains of
2964:
2820:
1697:
1183:
1158:
1012:
964:
905:
794:
789:
cells have to be produced immediately before use. Expression yields in
685:
406:
382:
359:
347:
291:– responsible for most, if not all, industrial & research use
237:
has become important for biological research and biotech applications.
1999:
635:
is able to grow on simple, inexpensive medium, with high growth rate.
502:, have similar growth conditions and tolerances; thus, the culture of
453:. In addition, all seven species were sequenced by 2022. Furthermore,
2846:
1658:
1641:
1078:
1059:
677:
647:, which makes it suitable for both small- and large-scale production.
488:
461:
cells, which means researchers could investigate the proteins inside
445:. The genome sequence and gene annotation can be browsed through the
394:
86:
2990:
2738:
2551:
Ali
Razaghi; Roger Huerlimann; Leigh Owens; Kirsten Heimann (2015).
2505:
2140:
920:
source, which is suitable for isotopic labelling applications, like
506:
can be adopted by labs without many modifications. Moreover, unlike
2794:
2106:
10.1002/1097-0290(20001005)70:1<1::AID-BIT1>3.0.CO;2-Y
832:
is also used as an expression system with similar advantages over
402:
279:(Guillierm., 1919) Y.Yamada, M.Matsuda, K.Maeda & Mikata, 1995
171:
2938:
310:(Dlauchy, Tornai-Leh., Fülöp & G.Péter, 2003) Kurtzman, 2005
246:
2742:
1004:
can degum high-phosphorus oils by breaking down phospholipids.
935:
have been used in several kinds of biotech industries, such as
2035:. Methods in Molecular Biology. Vol. 103. pp. 1–15.
1184:"Komagataella Y.Yamada, M.Matsuda, K.Maeda & Mikata, 1995"
1035:
YSH597 is based on strain NRRL-Y11430, now considered part of
1462:"Ancient Evolutionary Trade-Offs between Yeast Ploidy States"
882:
Comparing to other expression systems, such as S2-cells from
1423:
Kurtzman (1998). "42 - Pichia E.C. Hansen emend. Kurtzman".
916:
can grow in media containing only one carbon source and one
785:
cells can be stored frozen, and thawed before use, whereas
2467:. Methods in Enzymology. Vol. 463. pp. 131–47.
2426:. Methods in Enzymology. Vol. 463. pp. 169–89.
27:
Genus of fungus used industrially and as model organism
1919:"Pichia pastoris as a host system for transformations"
1259:
Journal of Industrial Microbiology & Biotechnology
951:
had been used for the production of over 500 types of
599:
suited for this task. Currently, several strains of
192:
was reassigned into the sole representative of genus
718:
The technology required for genetic manipulation of
680:
and energy source. The AOX promoters are induced by
472:
is its similarity to the well-studied yeast model —
269:
G.I.Naumov, E.S.Naumova & K.L.Boundy-Mills, 2018
2880:
2751:
988:is used to produce different kinds of enzymes, as
259:G.I.Naumov, E.S.Naumova, Tyurin & Kozlov, 2013
143:Y. Yamada, M. Matsuda, K. Maeda & Mikata, 1995
2309:Bankefa, OE; Wang, M; Zhu, T; Li, Y (July 2018).
984:In food industries, like brewery and bake house,
533:can be used for genetic analysis and large-scale
1199:
1197:
2686:
2684:
2417:
2415:
2413:
1292:
1290:
607:, leading to the selection of cells which are
565:as a transformation host. In the last decade,
896:usually gives much better yields. Generally,
615:. The technology for vector integration into
8:
2673:: CS1 maint: multiple names: authors list (
2294:: CS1 maint: multiple names: authors list (
2235:: CS1 maint: multiple names: authors list (
2071:: CS1 maint: multiple names: authors list (
1967:: CS1 maint: multiple names: authors list (
1902:: CS1 maint: multiple names: authors list (
1844:: CS1 maint: multiple names: authors list (
1785:: CS1 maint: multiple names: authors list (
1718:: CS1 maint: multiple names: authors list (
1587:: CS1 maint: multiple names: authors list (
1527:Kastilan, R., Boes, A., Spiegel, H. (2017).
1512:: CS1 maint: multiple names: authors list (
1408:: CS1 maint: multiple names: authors list (
1100:: CS1 maint: multiple names: authors list (
1058:De Schutter, K., Lin, Y., Tiels, P. (2009).
1147:Bioscience, Biotechnology, and Biochemistry
188:as a source of carbon and energy. In 1995,
2739:
2465:Guide to Protein Purification, 2nd Edition
2424:Guide to Protein Purification, 2nd Edition
42:
31:
2718:
2708:
2571:
2393:
2383:
2326:
2202:Journal of Bioscience and Bioengineering
2007:
1944:
1762:
1752:
1733:Tran, A., Nguyen, T., Nguyen, C. (2017).
1657:
1564:
1554:
1489:
1479:
1385:
1375:
1324:
1314:
1270:
1221:
1077:
967:structure, which is a potential cause of
752:Comparison with other expression systems
704:genome, producing heterologous proteins.
1253:Kurtzman, Cletus Paul (November 2009).
1047:
1028:
848:in laboratory and industrial settings:
2666:
2287:
2228:
2064:
1960:
1895:
1837:
1778:
1711:
1580:
1505:
1401:
1355:Applied and Environmental Microbiology
1178:
1176:
1093:
389:. In this case, two types of cells of
1118:Agricultural and Biological Chemistry
221:. With strong potential for being an
7:
3014:c150c448-86a1-4163-846d-8d8466b027ea
2857:e05e39a0-d273-44ac-bb09-46fe644f64c4
556:As an experimental model organism,
1814:10.1016/B978-0-12-407679-2.00004-1
595:proteins. Several properties make
25:
2522:10.1016/j.biologicals.2016.09.015
552:as an experimental model organism
2173:Current Opinion in Biotechnology
2129:Journal of Molecular Recognition
2093:Biotechnology and Bioengineering
1988:Biotechnology and Bioengineering
1802:Advances in Applied Microbiology
1606:Biotechnology and Bioengineering
1437:10.1016/B978-044481312-1/50046-0
63:
2560:European Pharmaceutical Journal
774:is usually faster than that in
178:. It was found in the 1960s as
1924:Molecular and Cellular Biology
1131:10.1080/00021369.1969.10859497
1:
2653:10.1016/j.jbiotec.2015.01.027
2473:10.1016/S0076-6879(09)63011-1
2432:10.1016/S0076-6879(09)63013-5
1862:The Yeasts: A Taxonomic Study
1425:The Yeasts: A Taxonomic Study
844:has two main advantages over
619:genome is similar to that in
580:as expression system platform
529:As a genetic model organism,
2215:10.1016/j.jbiosc.2014.03.009
2186:10.1016/0958-1669(95)80087-5
1481:10.1371/journal.pgen.1003388
358:. However, they cannot use
184:, with its feature of using
890:Chinese hamster ovary cells
855:, as mentioned above, is a
525:as a genetic model organism
342:is found on trees, such as
306:Komagataella pseudopastoris
3081:
2385:10.1038/s41598-017-16577-x
1556:10.1038/s41598-017-11819-4
1316:10.1186/s12934-022-01796-3
2328:10.1007/s10529-018-2571-y
1754:10.1186/s13104-017-2471-6
1272:10.1007/s10295-009-0638-4
943:Biotherapeutic production
739:As some proteins require
665:, which include strongly
587:is frequently used as an
153:
148:
60:Scientific classification
58:
50:
41:
34:
2641:Journal of Biotechnology
1303:Microbial Cell Factories
830:Saccharomyces cerevisiae
760:research, the bacterium
724:Saccharomyces cerevisiae
672:. These two genes allow
621:Saccharomyces cerevisiae
569:was engineered to build
475:Saccharomyces cerevisiae
437:had been performed. The
265:Komagataella mondaviorum
2610:10.1126/science.1130256
2573:10.1515/afpuc-2015-0031
2253:Molecular Biotechnology
2041:10.1385/0-89603-421-6:1
947:In the last few years,
937:pharmaceutical industry
928:Industrial applications
885:Drosophila melanogaster
793:vary between different
420:In the last few years,
255:Komagataella kurtzmanii
3065:Taxa described in 1995
1015:is used to break down
809:is capable of forming
722:is similar to that of
591:for the production of
397:cells. In the asexual
209:After years of study,
2315:Biotechnology Letters
1937:10.1128/MCB.5.12.3376
1874:10.1002/yea.320080805
963:with high density of
676:to use methanol as a
468:Another advantage of
275:Komagataella pastoris
229:, as well as being a
2710:10.3390/biom13030441
1982:Brady, J.R. (2020).
1646:Nature Biotechnology
1377:10.1128/AEM.00638-16
1223:10.1099/mic.0.000958
1065:Nature Biotechnology
743:for proper folding,
443:Nature Biotechnology
375:asexual reproduction
285:Komagataella phaffii
235:Komagataella phaffii
233:for genetic study,
199:Komagataella phaffii
52:Komagataella phaffii
2602:2006Sci...313.1441H
2376:2017NatSR...716249Y
2266:10.1385/MB:16:3:193
1685:Future Microbiology
1547:2017NatSR...711991K
1368:2016ApEnM..82.4570R
1019:, an antinutrient.
908:, as well as other
684:, and repressed by
639:can grow in either
416:as a model organism
395:haploid and diploid
296:Komagataella populi
3050:Saccharomycetaceae
2364:Scientific Reports
1698:10.2217/fmb.12.133
1159:10.1271/bbb.59.439
613:expression vectors
611:successfully with
227:protein production
219:biotech industries
213:is widely used in
3037:
3036:
3022:Open Tree of Life
2865:Open Tree of Life
2745:Taxon identifiers
2482:978-0-12-374536-1
2441:978-0-12-374536-1
2000:10.1002/bit.27209
1931:(12): 3376–3385.
1362:(15): 4570–4583.
1265:(11): 1435–1438.
1125:(10): 1519–1520.
980:Enzyme production
863:can grow fast in
828:The well-studied
817:in proteins, but
758:molecular biology
589:expression system
571:expression system
381:reproduction, by
373:can undergo both
316:Komagataella ulmi
223:expression system
176:Saccharomycetales
174:within the order
160:
159:
144:
117:Saccharomycetales
16:(Redirected from
3072:
3030:
3029:
3017:
3016:
3007:
3006:
2994:
2993:
2981:
2980:
2968:
2967:
2955:
2954:
2942:
2941:
2929:
2928:
2916:
2915:
2914:
2901:
2900:
2899:
2873:
2872:
2860:
2859:
2850:
2849:
2837:
2836:
2824:
2823:
2811:
2810:
2798:
2797:
2785:
2784:
2772:
2771:
2770:
2740:
2733:
2732:
2722:
2712:
2688:
2679:
2678:
2672:
2664:
2636:
2630:
2629:
2596:(5792): 1441–3.
2584:
2578:
2577:
2575:
2557:
2548:
2542:
2541:
2501:
2495:
2494:
2460:
2454:
2453:
2419:
2408:
2407:
2397:
2387:
2355:
2349:
2348:
2330:
2321:(7): 1149–1156.
2306:
2300:
2299:
2293:
2285:
2247:
2241:
2240:
2234:
2226:
2196:
2190:
2189:
2167:
2161:
2160:
2124:
2118:
2117:
2083:
2077:
2076:
2070:
2062:
2033:Pichia Protocols
2028:
2022:
2021:
2011:
1979:
1973:
1972:
1966:
1958:
1948:
1914:
1908:
1907:
1901:
1893:
1856:
1850:
1849:
1843:
1835:
1797:
1791:
1790:
1784:
1776:
1766:
1756:
1730:
1724:
1723:
1717:
1709:
1678:
1672:
1671:
1661:
1659:10.1038/nbt.1544
1637:
1631:
1630:
1599:
1593:
1592:
1586:
1578:
1568:
1558:
1524:
1518:
1517:
1511:
1503:
1493:
1483:
1457:
1451:
1450:
1420:
1414:
1413:
1407:
1399:
1389:
1379:
1345:
1339:
1338:
1328:
1318:
1294:
1285:
1284:
1274:
1250:
1244:
1243:
1225:
1201:
1192:
1191:
1180:
1171:
1170:
1141:
1135:
1134:
1112:
1106:
1105:
1099:
1091:
1081:
1079:10.1038/nbt.1544
1055:
1040:
1033:
1007:In animal feed,
778:, with reasons:
763:Escherichia coli
535:genetic crossing
321:
311:
301:
290:
280:
270:
260:
142:
127:Phaffomycetaceae
68:
67:
46:
32:
21:
3080:
3079:
3075:
3074:
3073:
3071:
3070:
3069:
3040:
3039:
3038:
3033:
3025:
3020:
3012:
3010:
3002:
2997:
2989:
2984:
2976:
2971:
2963:
2958:
2950:
2945:
2937:
2932:
2924:
2919:
2910:
2909:
2904:
2895:
2894:
2889:
2876:
2868:
2863:
2855:
2853:
2845:
2840:
2832:
2827:
2819:
2814:
2806:
2801:
2793:
2788:
2780:
2775:
2766:
2765:
2760:
2753:Pichia pastoris
2747:
2737:
2736:
2690:
2689:
2682:
2665:
2638:
2637:
2633:
2586:
2585:
2581:
2555:
2550:
2549:
2545:
2503:
2502:
2498:
2483:
2462:
2461:
2457:
2442:
2421:
2420:
2411:
2357:
2356:
2352:
2308:
2307:
2303:
2286:
2249:
2248:
2244:
2227:
2198:
2197:
2193:
2169:
2168:
2164:
2141:10.1002/jmr.687
2126:
2125:
2121:
2085:
2084:
2080:
2063:
2051:
2030:
2029:
2025:
1981:
1980:
1976:
1959:
1916:
1915:
1911:
1894:
1858:
1857:
1853:
1836:
1824:
1799:
1798:
1794:
1777:
1732:
1731:
1727:
1710:
1680:
1679:
1675:
1639:
1638:
1634:
1601:
1600:
1596:
1579:
1526:
1525:
1521:
1504:
1474:(3): e1003388.
1459:
1458:
1454:
1447:
1422:
1421:
1417:
1400:
1347:
1346:
1342:
1296:
1295:
1288:
1252:
1251:
1247:
1203:
1202:
1195:
1182:
1181:
1174:
1143:
1142:
1138:
1114:
1113:
1109:
1092:
1057:
1056:
1049:
1044:
1043:
1034:
1030:
1025:
1002:phospholipase C
990:processing aids
982:
953:biotherapeutics
945:
930:
865:cell suspension
754:
737:
655:alcohol oxidase
629:
582:
554:
527:
418:
368:
336:
334:Natural habitat
331:
319:
309:
299:
288:
278:
268:
258:
243:
181:Pichia pastoris
141:
107:Saccharomycetes
62:
28:
23:
22:
18:Pichia pastoris
15:
12:
11:
5:
3078:
3076:
3068:
3067:
3062:
3057:
3052:
3042:
3041:
3035:
3034:
3032:
3031:
3018:
3008:
2995:
2982:
2969:
2956:
2943:
2930:
2917:
2902:
2886:
2884:
2878:
2877:
2875:
2874:
2861:
2851:
2838:
2825:
2812:
2799:
2786:
2773:
2757:
2755:
2749:
2748:
2743:
2735:
2734:
2680:
2631:
2579:
2543:
2496:
2481:
2455:
2440:
2409:
2350:
2301:
2260:(3): 193–202.
2242:
2209:(4): 420–425.
2191:
2180:(5): 527–533.
2162:
2119:
2078:
2049:
2023:
1994:(2): 543–555.
1974:
1909:
1868:(8): 613–628.
1851:
1822:
1792:
1725:
1692:(2): 191–208.
1673:
1632:
1613:(3): 279–286.
1594:
1519:
1452:
1445:
1415:
1340:
1286:
1245:
1216:(7): 614–616.
1193:
1172:
1153:(3): 439–444.
1136:
1107:
1072:(6): 561–566.
1046:
1045:
1042:
1041:
1027:
1026:
1024:
1021:
994:food additives
981:
978:
973:erythropoietin
969:immunogenicity
944:
941:
929:
926:
910:growth factors
880:
879:
868:
815:glycosylations
753:
750:
736:
733:
732:
731:
716:
705:
648:
628:
625:
581:
575:
553:
547:
526:
520:
480:model organism
417:
411:
367:
364:
344:chestnut trees
335:
332:
330:
324:
323:
322:
320:Kurtzman, 2012
312:
302:
300:Kurtzman, 2012
292:
289:Kurtzman, 2005
281:
271:
261:
242:
239:
231:model organism
169:methylotrophic
158:
157:
151:
150:
146:
145:
134:
130:
129:
124:
120:
119:
114:
110:
109:
104:
100:
99:
94:
90:
89:
84:
80:
79:
74:
70:
69:
56:
55:
48:
47:
39:
38:
26:
24:
14:
13:
10:
9:
6:
4:
3:
2:
3077:
3066:
3063:
3061:
3058:
3056:
3055:Fungal models
3053:
3051:
3048:
3047:
3045:
3028:
3023:
3019:
3015:
3009:
3005:
3000:
2996:
2992:
2987:
2983:
2979:
2974:
2970:
2966:
2961:
2957:
2953:
2948:
2944:
2940:
2935:
2931:
2927:
2922:
2918:
2913:
2907:
2903:
2898:
2892:
2888:
2887:
2885:
2883:
2879:
2871:
2866:
2862:
2858:
2852:
2848:
2843:
2839:
2835:
2830:
2826:
2822:
2817:
2813:
2809:
2804:
2800:
2796:
2791:
2787:
2783:
2778:
2774:
2769:
2763:
2759:
2758:
2756:
2754:
2750:
2746:
2741:
2730:
2726:
2721:
2716:
2711:
2706:
2702:
2698:
2694:
2687:
2685:
2681:
2676:
2670:
2662:
2658:
2654:
2650:
2646:
2642:
2635:
2632:
2627:
2623:
2619:
2615:
2611:
2607:
2603:
2599:
2595:
2591:
2583:
2580:
2574:
2569:
2565:
2561:
2554:
2547:
2544:
2539:
2535:
2531:
2527:
2523:
2519:
2515:
2511:
2507:
2500:
2497:
2492:
2488:
2484:
2478:
2474:
2470:
2466:
2459:
2456:
2451:
2447:
2443:
2437:
2433:
2429:
2425:
2418:
2416:
2414:
2410:
2405:
2401:
2396:
2391:
2386:
2381:
2377:
2373:
2369:
2365:
2361:
2354:
2351:
2346:
2342:
2338:
2334:
2329:
2324:
2320:
2316:
2312:
2305:
2302:
2297:
2291:
2283:
2279:
2275:
2271:
2267:
2263:
2259:
2255:
2254:
2246:
2243:
2238:
2232:
2224:
2220:
2216:
2212:
2208:
2204:
2203:
2195:
2192:
2187:
2183:
2179:
2175:
2174:
2166:
2163:
2158:
2154:
2150:
2146:
2142:
2138:
2135:(2): 119–38.
2134:
2130:
2123:
2120:
2115:
2111:
2107:
2103:
2099:
2095:
2094:
2089:
2082:
2079:
2074:
2068:
2060:
2056:
2052:
2050:0-89603-421-6
2046:
2042:
2038:
2034:
2027:
2024:
2019:
2015:
2010:
2005:
2001:
1997:
1993:
1989:
1985:
1978:
1975:
1970:
1964:
1956:
1952:
1947:
1942:
1938:
1934:
1930:
1926:
1925:
1920:
1913:
1910:
1905:
1899:
1891:
1887:
1883:
1879:
1875:
1871:
1867:
1863:
1855:
1852:
1847:
1841:
1833:
1829:
1825:
1823:9780124076792
1819:
1815:
1811:
1807:
1803:
1796:
1793:
1788:
1782:
1774:
1770:
1765:
1760:
1755:
1750:
1746:
1742:
1741:
1740:BMC Res Notes
1736:
1729:
1726:
1721:
1715:
1707:
1703:
1699:
1695:
1691:
1687:
1686:
1677:
1674:
1669:
1665:
1660:
1655:
1651:
1647:
1643:
1636:
1633:
1628:
1624:
1620:
1616:
1612:
1608:
1607:
1598:
1595:
1590:
1584:
1576:
1572:
1567:
1562:
1557:
1552:
1548:
1544:
1540:
1536:
1535:
1530:
1523:
1520:
1515:
1509:
1501:
1497:
1492:
1487:
1482:
1477:
1473:
1469:
1468:
1467:PLOS Genetics
1463:
1456:
1453:
1448:
1446:9780444813121
1442:
1438:
1434:
1430:
1426:
1419:
1416:
1411:
1405:
1397:
1393:
1388:
1383:
1378:
1373:
1369:
1365:
1361:
1357:
1356:
1351:
1344:
1341:
1336:
1332:
1327:
1322:
1317:
1312:
1308:
1304:
1300:
1293:
1291:
1287:
1282:
1278:
1273:
1268:
1264:
1260:
1256:
1249:
1246:
1241:
1237:
1233:
1229:
1224:
1219:
1215:
1211:
1207:
1200:
1198:
1194:
1189:
1185:
1179:
1177:
1173:
1168:
1164:
1160:
1156:
1152:
1148:
1140:
1137:
1132:
1128:
1124:
1120:
1119:
1111:
1108:
1103:
1097:
1089:
1085:
1080:
1075:
1071:
1067:
1066:
1061:
1054:
1052:
1048:
1038:
1032:
1029:
1022:
1020:
1018:
1014:
1010:
1005:
1003:
999:
995:
991:
987:
979:
977:
974:
970:
966:
962:
961:glycosylation
958:
954:
950:
942:
940:
938:
934:
927:
925:
923:
919:
915:
911:
907:
903:
899:
895:
891:
887:
886:
877:
872:
869:
866:
862:
858:
854:
851:
850:
849:
847:
846:S. cerevisiae
843:
839:
835:
831:
826:
824:
820:
816:
812:
808:
804:
800:
796:
792:
788:
784:
781:
777:
773:
769:
765:
764:
759:
751:
749:
746:
742:
734:
729:
725:
721:
717:
714:
710:
706:
703:
699:
695:
691:
687:
683:
679:
675:
671:
668:
664:
660:
656:
652:
649:
646:
642:
638:
634:
631:
630:
626:
624:
622:
618:
614:
610:
606:
602:
598:
594:
590:
586:
579:
576:
574:
572:
568:
564:
559:
551:
548:
546:
544:
540:
536:
532:
524:
521:
519:
517:
513:
509:
508:S. cerevisiae
505:
501:
500:
499:Saccharomyces
495:
491:
490:
485:
484:S. cerevisiae
482:for biology,
481:
477:
476:
471:
466:
464:
460:
456:
452:
448:
444:
440:
436:
432:
427:
423:
415:
412:
410:
408:
404:
400:
396:
392:
388:
384:
380:
376:
372:
365:
363:
361:
357:
353:
349:
345:
341:
333:
328:
325:
318:
317:
313:
308:
307:
303:
298:
297:
293:
287:
286:
282:
277:
276:
272:
267:
266:
262:
257:
256:
252:
251:
250:
248:
245:According to
240:
238:
236:
232:
228:
224:
220:
217:research and
216:
212:
207:
205:
201:
200:
195:
191:
187:
183:
182:
177:
173:
170:
166:
165:
156:
152:
147:
140:
139:
135:
132:
131:
128:
125:
122:
121:
118:
115:
112:
111:
108:
105:
102:
101:
98:
95:
92:
91:
88:
85:
82:
81:
78:
75:
72:
71:
66:
61:
57:
53:
49:
45:
40:
37:
33:
30:
19:
2912:Komagataella
2882:Komagataella
2881:
2752:
2700:
2697:Biomolecules
2696:
2669:cite journal
2644:
2640:
2634:
2593:
2589:
2582:
2566:(2): 43–50.
2563:
2559:
2546:
2513:
2509:
2499:
2464:
2458:
2423:
2370:(1): 16249.
2367:
2363:
2353:
2318:
2314:
2304:
2290:cite journal
2257:
2251:
2245:
2231:cite journal
2206:
2200:
2194:
2177:
2171:
2165:
2132:
2128:
2122:
2097:
2091:
2081:
2032:
2026:
1991:
1987:
1977:
1963:cite journal
1928:
1922:
1912:
1898:cite journal
1865:
1861:
1854:
1840:cite journal
1805:
1801:
1795:
1781:cite journal
1744:
1738:
1728:
1714:cite journal
1689:
1683:
1676:
1652:(6): 561–6.
1649:
1645:
1635:
1610:
1604:
1597:
1583:cite journal
1538:
1532:
1522:
1508:cite journal
1471:
1465:
1455:
1428:
1424:
1418:
1404:cite journal
1359:
1353:
1343:
1306:
1302:
1262:
1258:
1248:
1213:
1210:Microbiology
1209:
1188:www.gbif.org
1187:
1150:
1146:
1139:
1122:
1116:
1110:
1096:cite journal
1069:
1063:
1036:
1031:
1008:
1006:
998:Komagataella
997:
986:Komagataella
985:
983:
949:Komagataella
948:
946:
933:Komagataella
932:
931:
914:Komagataella
913:
894:Komagataella
893:
883:
881:
876:Komagataella
875:
871:Komagataella
870:
861:Komagataella
860:
857:methylotroph
853:Komagataella
852:
845:
842:Komagataella
841:
838:Komagataella
837:
833:
829:
827:
822:
818:
807:Komagataella
806:
802:
799:Komagataella
798:
791:Komagataella
790:
787:Komagataella
786:
782:
776:Komagataella
775:
771:
761:
756:In standard
755:
745:Komagataella
744:
738:
735:Disadvantage
728:Komagataella
727:
723:
720:Komagataella
719:
713:Komagataella
712:
702:Komagataella
701:
698:Komagataella
697:
693:
689:
674:Komagataella
673:
662:
658:
651:Komagataella
650:
641:shake flasks
637:Komagataella
636:
633:Komagataella
632:
620:
617:Komagataella
616:
601:Komagataella
600:
597:Komagataella
596:
593:heterologous
585:Komagataella
584:
583:
578:Komagataella
577:
567:Komagataella
566:
563:Komagataella
562:
558:Komagataella
557:
555:
550:Komagataella
549:
543:Komagataella
542:
531:Komagataella
530:
528:
523:Komagataella
522:
516:Komagataella
515:
512:Komagataella
511:
507:
504:Komagataella
503:
497:
487:
483:
473:
470:Komagataella
469:
467:
463:Komagataella
462:
455:Komagataella
454:
451:Komagataella
450:
442:
438:
435:Komagataella
434:
431:Komagataella
430:
426:Komagataella
425:
422:Komagataella
421:
419:
414:Komagataella
413:
391:Komagataella
390:
371:Komagataella
370:
369:
366:Reproduction
340:Komagataella
339:
337:
327:Komagataella
326:
314:
304:
294:
283:
273:
263:
253:
244:
234:
211:Komagataella
210:
208:
203:
198:
197:
194:Komagataella
193:
189:
180:
179:
164:Komagataella
163:
162:
161:
154:
138:Komagataella
137:
136:
51:
36:Komagataella
35:
29:
2906:Wikispecies
2647:: 420–425.
2510:Biologicals
1808:: 115–153.
1431:: 273–352.
1017:phytic acid
922:protein NMR
902:amino acids
707:With a key
609:transformed
457:are single
403:sporulation
338:In nature,
215:biochemical
196:, becoming
190:P. pastoris
3044:Categories
2703:(3): 441.
2100:(1): 1–8.
1747:(1): 148.
1023:References
1011:-produced
955:, such as
898:cell lines
840:. However
813:bonds and
741:chaperonin
539:peroxisome
494:sensu lato
459:eukaryotic
439:K. phaffii
399:life cycle
155:See text
97:Ascomycota
93:Division:
2897:Q10547278
2516:: 52–60.
2067:cite book
1309:(1): 70.
1232:1350-0872
1037:K. phaffi
1009:K. phaffi
821:cannot.
811:disulfide
780:Competent
670:promoters
667:inducible
645:fermenter
627:Advantage
605:HIS4 gene
387:ascospore
329:in nature
83:Kingdom:
77:Eukaryota
2986:MycoBank
2947:Fungorum
2891:Wikidata
2842:MycoBank
2834:10780229
2803:Fungorum
2762:Wikidata
2729:36979376
2720:10046876
2661:25687104
2626:43334198
2618:16960007
2538:28204059
2530:27810255
2491:19892171
2450:19892173
2404:29176680
2345:29155989
2337:29785668
2282:22792748
2274:11252804
2223:24745549
2149:15565717
2114:10940857
2018:31654411
1832:23415154
1773:28376863
1706:23374125
1668:19465926
1627:18636643
1575:28931852
1541:(1): 7.
1500:23555297
1396:27208115
1335:35468837
1281:19760441
1240:32720891
1088:19465926
918:nitrogen
906:vitamins
805:is that
768:proteins
696:gene in
682:methanol
653:has two
356:methanol
352:glycerol
241:Taxonomy
186:methanol
149:Species
123:Family:
73:Domain:
2978:1012130
2965:2599347
2939:6655148
2821:2599390
2768:Q148697
2598:Bibcode
2590:Science
2395:5701153
2372:Bibcode
2157:7476149
2059:9680629
2009:7003935
1955:3915774
1890:8840145
1882:1441741
1764:5379694
1566:5607246
1543:Bibcode
1491:3605057
1387:4984280
1364:Bibcode
1326:9036795
1167:7766181
1013:phytase
965:mannose
834:E. coli
823:E. coli
819:E. coli
803:E. coli
783:E. coli
772:E. coli
686:glucose
657:genes,
478:. As a
407:meiosis
393:exist:
383:budding
360:lactose
348:glucose
133:Genus:
113:Order:
103:Class:
3060:Yeasts
3027:312891
3011:NZOR:
3004:460517
2870:333983
2854:NZOR:
2847:303634
2808:303634
2795:PICHPA
2727:
2717:
2659:
2624:
2616:
2536:
2528:
2489:
2479:
2448:
2438:
2402:
2392:
2343:
2335:
2280:
2272:
2221:
2155:
2147:
2112:
2057:
2047:
2016:
2006:
1953:
1946:369166
1943:
1888:
1880:
1830:
1820:
1771:
1761:
1704:
1666:
1625:
1573:
1563:
1534:Nature
1498:
1488:
1443:
1394:
1384:
1333:
1323:
1279:
1238:
1230:
1165:
1086:
795:clones
678:carbon
496:) and
489:Pichia
379:sexual
204:pichia
54:GS115
2991:27606
2973:IRMNG
2952:27606
2829:IRMNG
2782:6VJ3K
2622:S2CID
2556:(PDF)
2534:S2CID
2341:S2CID
2278:S2CID
2153:S2CID
1886:S2CID
801:over
709:trait
643:or a
447:ORCAE
172:yeast
167:is a
87:Fungi
2999:NCBI
2960:GBIF
2926:58SW
2816:GBIF
2790:EPPO
2725:PMID
2675:link
2657:PMID
2614:PMID
2526:PMID
2487:PMID
2477:ISBN
2446:PMID
2436:ISBN
2400:PMID
2333:PMID
2296:link
2270:PMID
2237:link
2219:PMID
2145:PMID
2110:PMID
2073:link
2055:PMID
2045:ISBN
2014:PMID
1969:link
1951:PMID
1904:link
1878:PMID
1846:link
1828:PMID
1818:ISBN
1787:link
1769:PMID
1720:link
1702:PMID
1664:PMID
1623:PMID
1589:link
1571:PMID
1514:link
1496:PMID
1441:ISBN
1410:link
1392:PMID
1331:PMID
1277:PMID
1236:PMID
1228:ISSN
1163:PMID
1102:link
1084:PMID
992:and
957:IFNγ
888:and
694:AOX1
690:Aox1
663:Aox2
661:and
659:Aox1
405:and
385:and
377:and
354:and
247:GBIF
225:for
2934:EoL
2921:CoL
2777:CoL
2715:PMC
2705:doi
2649:doi
2645:202
2606:doi
2594:313
2568:doi
2518:doi
2469:doi
2428:doi
2390:PMC
2380:doi
2323:doi
2262:doi
2211:doi
2207:118
2182:doi
2137:doi
2102:doi
2037:doi
2004:PMC
1996:doi
1992:117
1941:PMC
1933:doi
1870:doi
1810:doi
1759:PMC
1749:doi
1694:doi
1654:doi
1615:doi
1561:PMC
1551:doi
1486:PMC
1476:doi
1433:doi
1382:PMC
1372:doi
1321:PMC
1311:doi
1267:doi
1218:doi
1214:166
1155:doi
1127:doi
1074:doi
836:as
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3001::
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