3481:
383:. As Pol III stalls unable to add nucleotides to the nascent DNA strand, the cell becomes at risk of having the replication fork collapse and cell death to occur. Pol V TLS function depends on association with other elements of the SOS response, most importantly Pol V translesion activity is tightly dependent on the formation of
461:
Pol V is only expressed in the cell during the SOS response. It is very tightly regulated at different levels of protein expression and under different mechanisms to avoid its activity unless absolutely necessary for survival of the cell. Pol V's strict regulation stems from its poor replication
355:. The polymerase function of Pol V was not discovered until the late 1990s when UmuC was successfully extracted, consequent experiments unequivocally proved UmuD'2C is a polymerase. This finding lead to the detection of many Pol V
3008:
498:
The formation of the UmuD'2C complex is limited by the formation of UmuD' from UmuD. UmuD is made of a polypeptide with 139 amino acid residues that form a stable tertiary structure, however it needs to be
515:
UmuD'2C complex is inactive unless associated with RecA*. Pol V directly interacts with RecA* at the 3' tip of the nucleoprotein filament; this is the site of the nascent DNA strand where Pol V restarts
347:
can stall and lead to cell death. However, Y polymerases have low sequence fidelity during replication (prone to add wrong nucleotides). When the UmuC and UmuD' proteins were initially discovered in
462:
fidelity, Pol V is highly mutagenic and it is used as a last resort in DNA repair mechanisms. As such, the expression of the UmuD'2C complex takes 45–50 minutes after UV radiation exposure.
3018:
3013:
2173:
3133:
3111:
2033:
2929:
1773:
217:
79:
264:
126:
490:, which leads to the autocleavage of the repressor freeing the operon for transcription. The UmuDC operon is transcribed and translated into UmuC and UmuD.
3039:
482:
binds to the promoter of the UmuDC operon and inhibits gene transcription. DNA damage in the cell leads to the formation of RecA*. RecA* interacts with
2934:
1019:
3101:
1583:
1546:
351:, they were thought to be agents that inhibit faithful DNA replication and caused DNA synthesis to have high mutation rates after exposure to
2740:
1766:
387:
nucleoprotein filaments. Pol V can use TLS on lesions that block replication or miscoding lesions, which modify bases and lead to wrong
2874:
1666:
3200:
2444:
2238:
1675:
1241:
647:
2585:
1728:
375:
to DNA damage. When DNA is damaged regular DNA synthesis polymerases are unable to add dNTPs onto the newly synthesized strand.
210:
72:
500:
1759:
1588:
3356:
3158:
3153:
2713:
2531:
1398:
1248:
1012:
376:
335:, forming the UmuD'2C protein complex. It is part of the Y-family of DNA Polymerases, which are capable of performing DNA
3471:
503:
to be in its active form. UmuD has self-proteolytic activity that is activated by RecA, it removes 24 amino acids at the
3023:
2911:
2879:
2864:
1993:
2546:
1389:
1057:
1032:
1751:
1518:
1494:
1363:
1195:
1142:
1124:
1036:
3341:
3457:
3444:
3431:
3418:
3405:
3392:
3379:
3144:
3122:
3097:
3072:
2992:
2600:
2560:
2505:
2482:
2454:
2422:
2288:
1908:
1893:
1793:
1572:
3351:
2375:
925:"Suppression of Rev3, the catalytic subunit of Pol{zeta}, sensitizes drug-resistant lung tumors to chemotherapy"
507:, turning it into UmuD'. UmuD' can form a homodimer and associate with UmuC to form the active UmuD'2C complex.
3501:
3305:
3248:
2609:
2590:
2521:
2255:
1790:
1733:
1005:
246:
108:
185:
47:
3253:
2467:
2183:
2148:
2143:
1135:
1066:
720:
Jarosz DF, Beuning PJ, Cohen SE, Walker GC (February 2007). "Y-family DNA polymerases in
Escherichia coli".
2921:
2694:
2604:
1632:
1563:
396:
3274:
3193:
2775:
2730:
2432:
2417:
2353:
2333:
2231:
2138:
1988:
1558:
1327:
997:
336:
3346:
2216:
2689:
2684:
2564:
2427:
2407:
2316:
2128:
1605:
1578:
1529:
3310:
2952:
2944:
2725:
2392:
2328:
2292:
2028:
2018:
1835:
1830:
1335:
471:
415:
in the cell. The role of Pol V in SOS response triggered by UV-radiation is described as follows:
3511:
3243:
2815:
2753:
2704:
2439:
2266:
356:
3506:
2830:
2820:
2810:
2735:
2719:
2678:
2664:
2659:
2397:
2360:
2010:
1541:
1358:
977:
956:
905:
851:
799:
747:
697:
643:
620:
578:
391:. However, it is unable to translate through 5' → 3' backbone nick errors. Pol V also lacks
3289:
3284:
3258:
3186:
3163:
2825:
2805:
2643:
2541:
2526:
2472:
2370:
2365:
2224:
1802:
1498:
1347:
1342:
1234:
946:
936:
895:
887:
841:
833:
789:
781:
737:
729:
687:
679:
612:
568:
558:
427:
412:
344:
323:
547:"Managing DNA polymerases: coordinating DNA replication, DNA repair, and DNA recombination"
3336:
3320:
3233:
3086:
3076:
2898:
2869:
2495:
2402:
2387:
2043:
2038:
1810:
1786:
1711:
1503:
1377:
1095:
1045:
1028:
340:
284:
251:
146:
113:
3485:
3374:
3315:
3054:
2893:
2800:
2792:
2669:
2632:
1351:
1331:
951:
924:
900:
875:
846:
821:
794:
769:
692:
667:
483:
479:
475:
923:
Doles J, Oliver TG, Cameron ER, Hsu G, Jacks T, Walker GC, Hemann MT (November 2010).
770:"A new model for SOS-induced mutagenesis: how RecA protein activates DNA polymerase V"
616:
3495:
3279:
3238:
2323:
2188:
2061:
2050:
1782:
1553:
1480:
1090:
986:
573:
546:
524:/REV3L/REV7 pathway is necessary for the TLS synthesis mediated by DNA polymerase V.
517:
423:
328:
822:"An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η"
3228:
2412:
2382:
2311:
2084:
1978:
1820:
1692:
449:
Pol V accesses 3'-OH of nascent DNA strand and extends strand past the lesion site.
440:
372:
222:
84:
785:
3452:
3387:
3223:
2978:
2968:
2569:
2306:
2247:
2203:
2198:
1847:
1637:
891:
683:
487:
392:
352:
294:
156:
3480:
929:
Proceedings of the
National Academy of Sciences of the United States of America
551:
Proceedings of the
National Academy of Sciences of the United States of America
2780:
2623:
2536:
2301:
2251:
2165:
2107:
1888:
1883:
1863:
1723:
1697:
1485:
1470:
733:
504:
318:
314:
258:
229:
120:
91:
3426:
3400:
3059:
2972:
2509:
2275:
2193:
2179:
1998:
1878:
1868:
1661:
1475:
1465:
941:
446:
RecA functions through mediator proteins to activate Pol V (see
Regulation).
388:
960:
909:
855:
803:
751:
701:
624:
582:
563:
2906:
2486:
2079:
2074:
2023:
1873:
1858:
1825:
1738:
1719:
1291:
1282:
1080:
234:
96:
742:
2857:
2842:
2458:
2133:
2123:
2069:
1983:
1815:
1302:
1277:
1272:
1104:
991:
981:
430:
creating single stranded DNA (ssDNA) segments ahead of from the lesion.
332:
837:
3439:
3209:
2835:
2270:
2160:
2102:
205:
67:
17:
600:
367:
Pol V functions as a TLS (translesion DNA synthesis) polymerase in
3413:
2852:
2847:
2699:
2169:
2155:
2094:
1968:
1963:
1958:
1953:
1948:
1943:
1652:
1647:
1642:
1625:
1620:
1615:
1610:
1598:
1593:
1311:
1306:
1263:
1258:
1253:
3009:
CDP-diacylglycerol—glycerol-3-phosphate 3-phosphatidyltransferase
642:(1st Canadian ed.). Toronto: Nelson Education. p. 343.
443:
replacing SSBs. Formation of RecA nucleoprotein filament (RecA*).
2763:
2758:
2654:
2649:
2348:
2343:
2338:
1938:
1933:
1928:
1923:
1918:
1913:
1903:
1898:
1534:
1458:
1453:
1448:
1443:
1438:
1433:
1428:
1423:
1418:
1413:
1408:
1403:
1295:
1225:
1220:
1215:
1210:
1205:
1200:
1188:
1181:
1172:
1167:
1162:
1157:
1152:
1147:
1109:
1085:
1073:
521:
436:
384:
339:(TLS). Translesion polymerases bypass DNA damage lesions during
3182:
2220:
1755:
1001:
768:
Patel M, Jiang Q, Woodgate R, Cox MM, Goodman MF (June 2010).
976:
Overview of all the structural information available in the
3178:
876:"Translesion DNA synthesis and mutagenesis in prokaryotes"
774:
Critical
Reviews in Biochemistry and Molecular Biology
3469:
3019:
CDP-diacylglycerol—inositol 3-phosphatidyltransferase
3024:
CDP-diacylglycerol—choline O-phosphatidyltransferase
3365:
3329:
3298:
3267:
3216:
3143:
3121:
3096:
3071:
3052:
3032:
3014:
CDP-diacylglycerol—serine O-phosphatidyltransferase
3001:
2991:
2961:
2943:
2920:
2892:
2791:
2631:
2622:
2599:
2559:
2504:
2481:
2453:
2287:
2263:
2116:
2093:
2059:
2009:
1846:
1801:
1710:
1684:
1514:
1385:
1376:
1320:
1120:
1053:
1044:
290:
280:
275:
257:
245:
240:
228:
216:
204:
196:
180:
175:
170:
152:
142:
137:
119:
107:
102:
90:
78:
66:
58:
42:
37:
32:
359:and the discovery of the Y-family of polymerases.
411:attempts to alleviate the effect of a damaging
661:
659:
433:ssDNA binding proteins (SSBs) stabilize ssDNA.
343:- if a lesion is not repaired or bypassed the
3194:
2232:
1767:
1013:
715:
713:
711:
540:
538:
536:
8:
869:
867:
865:
763:
761:
3040:N-acetylglucosamine-1-phosphate transferase
2930:UTP—glucose-1-phosphate uridylyltransferase
815:
813:
520:. Additionally, it has been shown that the
470:Transcription of the SOS response genes is
379:(Pol III) is the regular DNA polymerase in
3201:
3187:
3179:
3068:
2998:
2628:
2619:
2284:
2239:
2225:
2217:
1774:
1760:
1752:
1382:
1050:
1020:
1006:
998:
880:Cold Spring Harbor Perspectives in Biology
672:Cold Spring Harbor Perspectives in Biology
272:
134:
3134:serine/threonine-specific protein kinases
3112:serine/threonine-specific protein kinases
2935:Galactose-1-phosphate uridylyltransferase
950:
940:
899:
845:
793:
741:
691:
572:
562:
399:synthesis causing it to be error prone.
3476:
666:Goodman MF, Woodgate R (October 2013).
532:
1787:serine proteases/serine endopeptidases
167:
29:
605:Current Opinion in Structural Biology
7:
594:
592:
874:Fuchs RP, Fujii S (December 2013).
439:recruited and loaded onto ssDNA by
395:activity, thus rendering unable to
545:Sutton MD, Walker GC (July 2001).
25:
2445:Glucose-1,6-bisphosphate synthase
1676:Control of chromosome duplication
1242:Autonomously replicating sequence
601:"Damage repair DNA polymerases Y"
3479:
2586:Ribose-phosphate diphosphokinase
321:mechanisms in bacteria, such as
990:(E. coli Protein UmuD) at the
419:Pol III stalls at lesion site.
1:
3159:Protein-histidine tele-kinase
3154:Protein-histidine pros-kinase
3033:Glycosyl-1-phosphotransferase
1999:Urinary plasminogen activator
1399:DNA polymerase III holoenzyme
1249:Single-strand binding protein
668:"Translesion DNA polymerases"
617:10.1016/S0959-440X(02)00003-9
501:post-translationally modified
494:Post-translational regulation
2865:RNA-dependent RNA polymerase
1994:Tissue plasminogen activator
786:10.3109/10409238.2010.480968
327:. It is composed of a UmuD'
2772:RNA-directed DNA polymerase
2640:DNA-directed DNA polymerase
892:10.1101/cshperspect.a012682
684:10.1101/cshperspect.a010363
452:Pol III resumes elongation.
171:DNA polymerase V, subunit D
33:DNA polymerase V, subunit C
3528:
3125:: protein-dual-specificity
1495:Prokaryotic DNA polymerase
1196:Minichromosome maintenance
1143:Origin recognition complex
466:Transcriptional regulation
190:(str. K-12 substr. MG1655)
52:(str. K-12 substr. MG1655)
3357:Michaelis–Menten kinetics
2085:Proteinase 3/Myeloblastin
1573:Eukaryotic DNA polymerase
734:10.1016/j.tim.2006.12.004
422:DNA replication helicase
271:
133:
27:Type of polymerase enzyme
3249:Diffusion-limited enzyme
3102:protein-serine/threonine
3002:Phosphatidyltransferases
2591:Thiamine diphosphokinase
599:Yang W (February 2003).
426:continues to expand the
1136:Pre-replication complex
1067:Pre-replication complex
942:10.1073/pnas.1011409107
2922:Nucleotidyltransferase
2605:nucleotidyltransferase
2532:Nucleoside-diphosphate
2139:Proprotein convertases
722:Trends in Microbiology
564:10.1073/pnas.111036998
265:genome: 1.23 - 1.23 Mb
127:genome: 1.23 - 1.23 Mb
3342:Eadie–Hofstee diagram
3275:Allosteric regulation
2776:Reverse transcriptase
1989:Plasminogen activator
1559:Replication protein A
1328:Origin of replication
511:Functional regulation
337:translesion synthesis
3352:Lineweaver–Burk plot
2565:diphosphotransferase
2547:Thiamine-diphosphate
2254:-containing groups (
2129:Prolyl endopeptidase
1530:Replication factor C
488:proteolytic activity
472:negatively regulated
3147:: protein-histidine
3065:; protein acceptor)
2953:mRNA capping enzyme
2945:Guanylyltransferase
820:Yang W (May 2014).
638:Garrett RH (2013).
486:and stimulates its
317:enzyme involved in
3311:Enzyme superfamily
3244:Enzyme promiscuity
2423:Phosphoinositide 3
2267:phosphotransferase
377:DNA Polymerase III
3467:
3466:
3176:
3175:
3172:
3171:
3048:
3047:
2987:
2986:
2888:
2887:
2801:Template-directed
2555:
2554:
2522:Phosphomevalonate
2214:
2213:
2011:Complement system
1803:Digestive enzymes
1749:
1748:
1706:
1705:
1542:Flap endonuclease
1372:
1371:
1359:Okazaki fragments
838:10.1021/bi500019s
304:
303:
300:
299:
166:
165:
162:
161:
16:(Redirected from
3519:
3484:
3483:
3475:
3347:Hanes–Woolf plot
3290:Enzyme activator
3285:Enzyme inhibitor
3259:Enzyme catalysis
3203:
3196:
3189:
3180:
3164:Histidine kinase
3087:tyrosine kinases
3077:protein-tyrosine
3069:
2999:
2806:RNA polymerase I
2629:
2620:
2473:Aspartate kinase
2468:Phosphoglycerate
2285:
2241:
2234:
2227:
2218:
1776:
1769:
1762:
1753:
1499:DNA polymerase I
1383:
1343:Replication fork
1235:Licensing factor
1051:
1022:
1015:
1008:
999:
965:
964:
954:
944:
935:(48): 20786–91.
920:
914:
913:
903:
871:
860:
859:
849:
832:(17): 2793–803.
817:
808:
807:
797:
765:
756:
755:
745:
717:
706:
705:
695:
663:
654:
653:
635:
629:
628:
596:
587:
586:
576:
566:
542:
428:replication fork
407:SOS response in
345:replication fork
324:Escherichia coli
307:DNA Polymerase V
273:
192:
187:Escherichia coli
168:
135:
54:
49:Escherichia coli
30:
21:
3527:
3526:
3522:
3521:
3520:
3518:
3517:
3516:
3502:DNA replication
3492:
3491:
3490:
3478:
3470:
3468:
3463:
3375:Oxidoreductases
3361:
3337:Enzyme kinetics
3325:
3321:List of enzymes
3294:
3263:
3234:Catalytic triad
3212:
3207:
3177:
3168:
3139:
3117:
3092:
3063:
3057:
3053:2.7.10-2.7.13:
3044:
3028:
2995:: miscellaneous
2983:
2957:
2939:
2916:
2899:exoribonuclease
2896:
2884:
2870:Polyadenylation
2787:
2613:
2607:
2595:
2577:
2573:
2567:
2551:
2513:
2500:
2477:
2449:
2279:
2273:
2265:
2259:
2245:
2215:
2210:
2112:
2089:
2055:
2005:
1842:
1811:Enteropeptidase
1797:
1780:
1750:
1745:
1702:
1680:
1520:
1516:
1510:
1504:Klenow fragment
1387:
1368:
1352:leading strands
1316:
1126:
1122:
1116:
1055:
1040:
1029:DNA replication
1026:
973:
968:
922:
921:
917:
886:(12): a012682.
873:
872:
863:
819:
818:
811:
767:
766:
759:
719:
718:
709:
678:(10): a010363.
665:
664:
657:
650:
637:
636:
632:
598:
597:
590:
544:
543:
534:
530:
513:
496:
468:
459:
405:
371:as part of the
365:
341:DNA replication
189:
184:
51:
46:
28:
23:
22:
15:
12:
11:
5:
3525:
3523:
3515:
3514:
3509:
3504:
3494:
3493:
3489:
3488:
3465:
3464:
3462:
3461:
3448:
3435:
3422:
3409:
3396:
3383:
3369:
3367:
3363:
3362:
3360:
3359:
3354:
3349:
3344:
3339:
3333:
3331:
3327:
3326:
3324:
3323:
3318:
3313:
3308:
3302:
3300:
3299:Classification
3296:
3295:
3293:
3292:
3287:
3282:
3277:
3271:
3269:
3265:
3264:
3262:
3261:
3256:
3251:
3246:
3241:
3236:
3231:
3226:
3220:
3218:
3214:
3213:
3208:
3206:
3205:
3198:
3191:
3183:
3174:
3173:
3170:
3169:
3167:
3166:
3161:
3156:
3150:
3148:
3141:
3140:
3138:
3137:
3128:
3126:
3119:
3118:
3116:
3115:
3106:
3104:
3094:
3093:
3091:
3090:
3081:
3079:
3066:
3061:
3055:protein kinase
3050:
3049:
3046:
3045:
3043:
3042:
3036:
3034:
3030:
3029:
3027:
3026:
3021:
3016:
3011:
3005:
3003:
2996:
2989:
2988:
2985:
2984:
2982:
2981:
2976:
2965:
2963:
2959:
2958:
2956:
2955:
2949:
2947:
2941:
2940:
2938:
2937:
2932:
2926:
2924:
2918:
2917:
2915:
2914:
2909:
2903:
2901:
2894:Phosphorolytic
2890:
2889:
2886:
2885:
2883:
2882:
2877:
2872:
2867:
2862:
2861:
2860:
2855:
2850:
2840:
2839:
2838:
2828:
2823:
2818:
2813:
2808:
2803:
2797:
2795:
2793:RNA polymerase
2789:
2788:
2786:
2785:
2784:
2783:
2773:
2769:
2768:
2767:
2766:
2761:
2756:
2745:
2744:
2743:
2738:
2733:
2728:
2717:
2711:
2710:
2709:
2702:
2697:
2692:
2687:
2676:
2675:
2674:
2667:
2662:
2657:
2652:
2641:
2637:
2635:
2633:DNA polymerase
2626:
2617:
2611:
2597:
2596:
2594:
2593:
2588:
2582:
2580:
2575:
2571:
2557:
2556:
2553:
2552:
2550:
2549:
2544:
2539:
2534:
2529:
2524:
2518:
2516:
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2451:
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2437:
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2435:
2430:
2420:
2418:Diacylglycerol
2415:
2410:
2405:
2400:
2395:
2390:
2385:
2380:
2379:
2378:
2368:
2363:
2358:
2357:
2356:
2351:
2346:
2341:
2336:
2329:Phosphofructo-
2326:
2321:
2320:
2319:
2309:
2304:
2298:
2296:
2282:
2277:
2261:
2260:
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2229:
2221:
2212:
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2209:
2208:
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2201:
2191:
2186:
2177:
2163:
2158:
2153:
2152:
2151:
2146:
2136:
2131:
2126:
2120:
2118:
2114:
2113:
2111:
2110:
2105:
2099:
2097:
2091:
2090:
2088:
2087:
2082:
2077:
2072:
2066:
2064:
2057:
2056:
2054:
2053:
2048:
2047:
2046:
2041:
2031:
2026:
2021:
2015:
2013:
2007:
2006:
2004:
2003:
2002:
2001:
1996:
1986:
1974:
1973:
1972:
1971:
1966:
1961:
1956:
1951:
1946:
1941:
1936:
1931:
1926:
1921:
1916:
1911:
1906:
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1840:
1839:
1838:
1833:
1823:
1818:
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1807:
1805:
1799:
1798:
1783:Endopeptidases
1781:
1779:
1778:
1771:
1764:
1756:
1747:
1746:
1744:
1743:
1742:
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1539:
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1506:
1491:
1490:
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1473:
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1395:
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1299:
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1213:
1208:
1203:
1192:
1191:
1185:
1184:
1178:
1177:
1176:
1175:
1170:
1165:
1160:
1155:
1150:
1139:
1138:
1132:
1130:
1125:preparation in
1118:
1117:
1115:
1114:
1113:
1112:
1101:
1100:
1099:
1098:
1093:
1088:
1077:
1076:
1070:
1069:
1063:
1061:
1048:
1042:
1041:
1027:
1025:
1024:
1017:
1010:
1002:
996:
995:
972:
971:External links
969:
967:
966:
915:
861:
809:
757:
707:
655:
648:
630:
588:
557:(15): 8342–9.
531:
529:
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512:
509:
495:
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467:
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3345:
3343:
3340:
3338:
3335:
3334:
3332:
3328:
3322:
3319:
3317:
3316:Enzyme family
3314:
3312:
3309:
3307:
3304:
3303:
3301:
3297:
3291:
3288:
3286:
3283:
3281:
3280:Cooperativity
3278:
3276:
3273:
3272:
3270:
3266:
3260:
3257:
3255:
3252:
3250:
3247:
3245:
3242:
3240:
3239:Oxyanion hole
3237:
3235:
3232:
3230:
3227:
3225:
3222:
3221:
3219:
3215:
3211:
3204:
3199:
3197:
3192:
3190:
3185:
3184:
3181:
3165:
3162:
3160:
3157:
3155:
3152:
3151:
3149:
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3136:
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3127:
3124:
3120:
3114:
3113:
3108:
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3099:
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3089:
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3041:
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3025:
3022:
3020:
3017:
3015:
3012:
3010:
3007:
3006:
3004:
3000:
2997:
2994:
2990:
2980:
2977:
2974:
2970:
2967:
2966:
2964:
2960:
2954:
2951:
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2948:
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2942:
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2928:
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2900:
2895:
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2881:
2878:
2876:
2873:
2871:
2868:
2866:
2863:
2859:
2856:
2854:
2851:
2849:
2846:
2845:
2844:
2841:
2837:
2834:
2833:
2832:
2829:
2827:
2824:
2822:
2819:
2817:
2814:
2812:
2809:
2807:
2804:
2802:
2799:
2798:
2796:
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2790:
2782:
2779:
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2737:
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2729:
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2724:
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2718:
2715:
2712:
2708:
2707:
2703:
2701:
2698:
2696:
2693:
2691:
2688:
2686:
2683:
2682:
2680:
2677:
2673:
2672:
2668:
2666:
2663:
2661:
2658:
2656:
2653:
2651:
2648:
2647:
2645:
2642:
2639:
2638:
2636:
2634:
2630:
2627:
2625:
2621:
2618:
2615:
2606:
2602:
2598:
2592:
2589:
2587:
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2583:
2581:
2578:
2566:
2562:
2558:
2548:
2545:
2543:
2540:
2538:
2535:
2533:
2530:
2528:
2525:
2523:
2520:
2519:
2517:
2514:
2507:
2503:
2497:
2494:
2493:
2491:
2488:
2484:
2480:
2474:
2471:
2469:
2466:
2465:
2463:
2460:
2456:
2452:
2446:
2443:
2441:
2438:
2434:
2433:Class II PI 3
2431:
2429:
2426:
2425:
2424:
2421:
2419:
2416:
2414:
2411:
2409:
2408:Deoxycytidine
2406:
2404:
2401:
2399:
2396:
2394:
2391:
2389:
2386:
2384:
2381:
2377:
2376:ADP-thymidine
2374:
2373:
2372:
2369:
2367:
2364:
2362:
2359:
2355:
2352:
2350:
2347:
2345:
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2327:
2325:
2322:
2318:
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2303:
2300:
2299:
2297:
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2283:
2280:
2272:
2268:
2262:
2257:
2253:
2249:
2242:
2237:
2235:
2230:
2228:
2223:
2222:
2219:
2205:
2202:
2200:
2197:
2196:
2195:
2192:
2190:
2189:Streptokinase
2187:
2185:
2181:
2178:
2175:
2171:
2167:
2164:
2162:
2159:
2157:
2154:
2150:
2147:
2145:
2142:
2141:
2140:
2137:
2135:
2132:
2130:
2127:
2125:
2122:
2121:
2119:
2115:
2109:
2106:
2104:
2101:
2100:
2098:
2096:
2092:
2086:
2083:
2081:
2078:
2076:
2073:
2071:
2068:
2067:
2065:
2063:
2062:immune system
2058:
2052:
2051:C3-convertase
2049:
2045:
2042:
2040:
2037:
2036:
2035:
2032:
2030:
2027:
2025:
2022:
2020:
2017:
2016:
2014:
2012:
2008:
2000:
1997:
1995:
1992:
1991:
1990:
1987:
1985:
1982:
1980:
1976:
1975:
1970:
1967:
1965:
1962:
1960:
1957:
1955:
1952:
1950:
1947:
1945:
1942:
1940:
1937:
1935:
1932:
1930:
1927:
1925:
1922:
1920:
1917:
1915:
1912:
1910:
1907:
1905:
1902:
1900:
1897:
1895:
1892:
1891:
1890:
1887:
1885:
1882:
1880:
1877:
1875:
1872:
1870:
1867:
1865:
1862:
1860:
1857:
1854:
1853:
1851:
1849:
1845:
1837:
1834:
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1829:
1828:
1827:
1824:
1822:
1819:
1817:
1814:
1812:
1809:
1808:
1806:
1804:
1800:
1795:
1792:
1788:
1784:
1777:
1772:
1770:
1765:
1763:
1758:
1757:
1754:
1740:
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1732:
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1727:
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1718:
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1709:
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1668:
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1631:
1627:
1624:
1622:
1619:
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1614:
1612:
1609:
1608:
1607:
1604:
1600:
1597:
1595:
1592:
1590:
1587:
1585:
1582:
1581:
1580:
1577:
1574:
1571:
1570:
1565:
1562:
1561:
1560:
1557:
1555:
1554:Topoisomerase
1552:
1548:
1545:
1544:
1543:
1540:
1536:
1533:
1532:
1531:
1528:
1527:
1525:
1522:
1513:
1505:
1502:
1501:
1500:
1496:
1493:
1492:
1487:
1484:
1483:
1482:
1481:Topoisomerase
1479:
1477:
1474:
1472:
1469:
1467:
1464:
1460:
1457:
1455:
1452:
1450:
1447:
1445:
1442:
1440:
1437:
1435:
1432:
1430:
1427:
1425:
1422:
1420:
1417:
1415:
1412:
1410:
1407:
1405:
1402:
1401:
1400:
1397:
1396:
1394:
1391:
1384:
1381:
1379:
1375:
1365:
1362:
1360:
1357:
1353:
1349:
1346:
1345:
1344:
1341:
1340:
1337:
1333:
1329:
1326:
1325:
1323:
1319:
1313:
1310:
1308:
1304:
1301:
1300:
1297:
1293:
1290:
1289:
1284:
1281:
1279:
1276:
1275:
1274:
1271:
1270:
1265:
1262:
1260:
1257:
1255:
1252:
1251:
1250:
1247:
1246:
1243:
1240:
1239:
1236:
1233:
1232:
1227:
1224:
1222:
1219:
1217:
1214:
1212:
1209:
1207:
1204:
1202:
1199:
1198:
1197:
1194:
1193:
1190:
1187:
1186:
1183:
1180:
1179:
1174:
1171:
1169:
1166:
1164:
1161:
1159:
1156:
1154:
1151:
1149:
1146:
1145:
1144:
1141:
1140:
1137:
1134:
1133:
1131:
1128:
1119:
1111:
1108:
1107:
1106:
1103:
1102:
1097:
1094:
1092:
1089:
1087:
1084:
1083:
1082:
1079:
1078:
1075:
1072:
1071:
1068:
1065:
1064:
1062:
1059:
1052:
1049:
1047:
1043:
1038:
1034:
1030:
1023:
1018:
1016:
1011:
1009:
1004:
1003:
1000:
993:
989:
988:
983:
979:
975:
974:
970:
962:
958:
953:
948:
943:
938:
934:
930:
926:
919:
916:
911:
907:
902:
897:
893:
889:
885:
881:
877:
870:
868:
866:
862:
857:
853:
848:
843:
839:
835:
831:
827:
823:
816:
814:
810:
805:
801:
796:
791:
787:
783:
780:(3): 171–84.
779:
775:
771:
764:
762:
758:
753:
749:
744:
739:
735:
731:
727:
723:
716:
714:
712:
708:
703:
699:
694:
689:
685:
681:
677:
673:
669:
662:
660:
656:
651:
649:9780176502652
645:
641:
634:
631:
626:
622:
618:
614:
610:
606:
602:
595:
593:
589:
584:
580:
575:
570:
565:
560:
556:
552:
548:
541:
539:
537:
533:
527:
525:
523:
519:
518:DNA synthesis
510:
508:
506:
502:
493:
491:
489:
485:
481:
477:
473:
465:
463:
456:
451:
448:
445:
442:
438:
435:
432:
429:
425:
421:
418:
417:
416:
414:
410:
402:
400:
398:
394:
390:
386:
382:
378:
374:
370:
362:
360:
358:
354:
350:
346:
342:
338:
334:
330:
326:
325:
320:
316:
312:
308:
296:
293:
289:
286:
283:
279:
274:
270:
267:
266:
262:
260:
256:
253:
250:
248:
244:
239:
236:
233:
231:
227:
224:
221:
219:
218:RefSeq (Prot)
215:
212:
209:
207:
203:
199:
195:
191:
188:
183:
179:
174:
169:
158:
155:
151:
148:
145:
141:
136:
132:
129:
128:
124:
122:
118:
115:
112:
110:
106:
101:
98:
95:
93:
89:
86:
83:
81:
80:RefSeq (Prot)
77:
74:
71:
69:
65:
61:
57:
53:
50:
45:
41:
36:
31:
19:
3453:Translocases
3450:
3437:
3424:
3411:
3398:
3388:Transferases
3385:
3372:
3229:Binding site
3131:
3109:
3084:
2747:
2705:
2670:
2428:Class I PI 3
2393:Pantothenate
2264:2.7.1-2.7.4:
2248:Transferases
1979:fibrinolysis
1977:
1855:
1821:Chymotrypsin
1693:Processivity
1519:synthesis in
985:
932:
928:
918:
883:
879:
829:
826:Biochemistry
825:
777:
773:
743:1721.1/70041
725:
721:
675:
671:
640:Biochemistry
639:
633:
611:(1): 23–30.
608:
604:
554:
550:
514:
497:
469:
460:
408:
406:
403:SOS Response
389:base pairing
380:
373:SOS response
368:
366:
348:
322:
310:
306:
305:
263:
186:
125:
48:
3224:Active site
2979:Transposase
2969:Recombinase
2614:-nucleoside
2440:Sphingosine
1884:Factor XIIa
1864:Factor VIIa
1848:Coagulation
1712:Termination
1386:Prokaryotic
1378:Replication
1054:Prokaryotic
1033:prokaryotic
1031:(comparing
728:(2): 70–7.
478:repressor.
393:exonuclease
331:and a UmuC
285:Swiss-model
223:NP_415701.1
176:Identifiers
147:Swiss-model
85:NP_415702.1
38:Identifiers
3496:Categories
3427:Isomerases
3401:Hydrolases
3268:Regulation
2781:Telomerase
2624:Polymerase
2398:Mevalonate
2361:Riboflavin
2252:phosphorus
2166:Subtilisin
2108:Batroxobin
1889:Kallikrein
1879:Factor XIa
1869:Factor IXa
1836:Pancreatic
1831:Neutrophil
1724:Telomerase
1698:DNA ligase
1691:Movement:
1515:Eukaryotic
1486:DNA gyrase
1471:DNA ligase
1390:elongation
1121:Eukaryotic
1058:initiation
1046:Initiation
1037:eukaryotic
528:References
505:N-terminus
457:Regulation
319:DNA repair
315:polymerase
281:Structures
276:Search for
259:Chromosome
241:Other data
143:Structures
138:Search for
121:Chromosome
103:Other data
3512:EC 3.4.21
3306:EC number
2973:Integrase
2897:3' to 5'
2542:Guanylate
2537:Uridylate
2527:Adenylate
2371:Thymidine
2366:Shikimate
2194:Cathepsin
2180:Sedolisin
2156:Prostasin
1874:Factor Xa
1662:DNA clamp
1476:DNA clamp
1466:Replisome
397:proofread
357:orthologs
329:homodimer
247:EC number
109:EC number
3507:EC 2.7.7
3330:Kinetics
3254:Cofactor
3217:Activity
2907:RNase PH
2515:acceptor
2496:Creatine
2489:acceptor
2461:acceptor
2403:Pyruvate
2388:Glycerol
2349:Platelet
2324:Galacto-
2295:acceptor
2095:Venombin
2080:Tryptase
2075:Granzyme
2029:Factor I
2024:Factor D
2019:Factor B
1859:Thrombin
1856:factors:
1826:Elastase
1720:Telomere
1336:Replicon
1292:Helicase
1283:RNASEH2A
1127:G1 phase
1081:Helicase
961:21068376
910:24296168
856:24716551
804:20441441
752:17207624
702:23838442
625:12581656
583:11459973
363:Function
353:UV-light
295:InterPro
252:3.4.21.-
181:Organism
157:InterPro
43:Organism
3486:Biology
3440:Ligases
3210:Enzymes
2858:PrimPol
2843:Primase
2317:Hepatic
2312:Fructo-
2134:Pronase
2124:Acrosin
2070:Chymase
1984:Plasmin
1816:Trypsin
1633:epsilon
1521:S phase
1348:Lagging
1303:Primase
1278:RNASEH1
1273:RNase H
1105:Primase
992:PDBe-KB
982:UniProt
952:2996428
901:3839610
847:4018060
795:2874081
693:3783050
474:by the
409:E. coli
381:E. coli
369:E. coli
349:E. coli
333:monomer
313:) is a
291:Domains
230:UniProt
153:Domains
114:2.7.7.7
92:UniProt
3472:Portal
3414:Lyases
3145:2.7.13
3123:2.7.12
3098:2.7.11
3073:2.7.10
2912:PNPase
2880:PNPase
2836:POLRMT
2831:ssRNAP
2344:Muscle
2307:Gluco-
2271:kinase
2161:Reelin
2103:Ancrod
2060:Other
1794:3.4.21
1364:Primer
987:P0AG11
959:
949:
908:
898:
854:
844:
802:
792:
750:
700:
690:
646:
623:
581:
571:
441:RecFOR
413:stress
235:P0AG11
211:945746
206:Entrez
197:Symbol
97:P04152
73:946359
68:Entrez
59:Symbol
3366:Types
2993:2.7.8
2962:Other
2601:2.7.7
2561:2.7.6
2506:2.7.4
2483:2.7.3
2455:2.7.2
2339:Liver
2302:Hexo-
2289:2.7.1
2170:Furin
2117:Other
2044:MASP2
2039:MASP1
1969:KLK15
1964:KLK14
1959:KLK13
1954:KLK12
1949:KLK11
1944:KLK10
1653:POLE4
1648:POLE3
1643:POLE2
1626:POLD4
1621:POLD3
1616:POLD2
1611:POLD1
1606:delta
1599:PRIM2
1594:PRIM1
1589:POLA2
1584:POLA1
1579:alpha
1312:PRIM2
1307:PRIM1
1264:SSBP4
1259:SSBP3
1254:SSBP2
574:37441
311:Pol V
18:Pol V
3458:list
3451:EC7
3445:list
3438:EC6
3432:list
3425:EC5
3419:list
3412:EC4
3406:list
3399:EC3
3393:list
3386:EC2
3380:list
3373:EC1
3132:see
3110:see
3085:see
2459:COOH
2258:2.7)
2184:TPP1
2034:MASP
1939:KLK9
1934:KLK8
1929:KLK7
1924:KLK6
1919:KLK5
1914:KLK4
1909:KLK3
1904:KLK2
1899:KLK1
1739:DKC1
1734:TERC
1729:TERT
1685:Both
1667:PCNA
1638:POLE
1564:RPA1
1547:FEN1
1535:RFC1
1459:holE
1454:holD
1449:holC
1444:holB
1439:holA
1434:dnaX
1429:dnaT
1424:dnaQ
1419:dnaN
1414:dnaH
1409:dnaE
1404:dnaC
1350:and
1321:Both
1296:HFM1
1226:MCM7
1221:MCM6
1216:MCM5
1211:MCM4
1206:MCM3
1201:MCM2
1189:Cdt1
1182:Cdc6
1173:ORC6
1168:ORC5
1163:ORC4
1158:ORC3
1153:ORC2
1148:ORC1
1110:dnaG
1091:dnaB
1086:dnaA
1074:dnaC
980:for
957:PMID
906:PMID
852:PMID
800:PMID
748:PMID
698:PMID
644:ISBN
621:PMID
579:PMID
522:REV1
484:LexA
480:LexA
476:LexA
437:RecA
424:DnaB
385:RecA
200:umuD
62:umuC
2875:PAP
2816:III
2750:/Y
2741:TDT
2722:/X
2714:III
2706:Pfu
2681:/B
2671:Taq
2646:/A
2413:PFP
2383:NAD
2174:S1P
1894:PSA
1332:Ori
1035:to
978:PDB
947:PMC
937:doi
933:107
896:PMC
888:doi
842:PMC
834:doi
790:PMC
782:doi
738:hdl
730:doi
688:PMC
680:doi
613:doi
569:PMC
559:doi
3498::
3100::
3075::
3060:PO
2821:IV
2811:II
2720:IV
2716:/C
2679:II
2665:T7
2610:PO
2603::
2563::
2510:PO
2508::
2485::
2457::
2293:OH
2291::
2276:PO
2256:EC
2250::
1791:EC
1785::
1722::
1497::
1305::
1294::
1096:T7
984::
955:.
945:.
931:.
927:.
904:.
894:.
882:.
878:.
864:^
850:.
840:.
830:53
828:.
824:.
812:^
798:.
788:.
778:45
776:.
772:.
760:^
746:.
736:.
726:15
724:.
710:^
696:.
686:.
674:.
670:.
658:^
619:.
609:13
607:.
603:.
591:^
577:.
567:.
555:98
553:.
549:.
535:^
3474::
3460:)
3456:(
3447:)
3443:(
3434:)
3430:(
3421:)
3417:(
3408:)
3404:(
3395:)
3391:(
3382:)
3378:(
3202:e
3195:t
3188:v
3062:4
3058:(
2975:)
2971:(
2853:2
2848:1
2826:V
2764:Îş
2759:Îą
2754:η
2748:V
2736:ÎĽ
2731:λ
2726:β
2700:ζ
2695:ε
2690:δ
2685:α
2660:ν
2655:θ
2650:Îł
2644:I
2616:)
2612:4
2608:(
2579:)
2576:7
2574:O
2572:2
2570:P
2568:(
2512:4
2487:N
2354:2
2334:1
2281:)
2278:4
2274:(
2269:/
2240:e
2233:t
2226:v
2204:G
2199:A
2182:/
2176:4
2172:/
2168:/
2149:2
2144:1
1981::
1796:)
1789:(
1775:e
1768:t
1761:v
1575::
1523:)
1517:(
1392:)
1388:(
1334:/
1330:/
1129:)
1123:(
1060:)
1056:(
1039:)
1021:e
1014:t
1007:v
994:.
963:.
939::
912:.
890::
884:5
858:.
836::
806:.
784::
754:.
740::
732::
704:.
682::
676:5
652:.
627:.
615::
585:.
561::
309:(
20:)
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