Knowledge (XXG)

Potato dextrose agar

Source đź“ť

43: 33: 1089: 96:
Potato dextrose agar is a versatile growing medium for bacteria and fungi (yeasts and molds). This agar is used for a broad range of fungi but there are other agars that are more selective for specific types of fungi. These agars include but are not limited to malt extract agar and
198:
is added such that the total volume of the suspension is 1 litre (0.22 imp gal; 0.26 US gal). 20 grams (0.71 oz) dextrose and 20 grams (0.71 oz) agar powder is then added and the medium is sterilized by
185:
Potato infusion can be made by boiling 200 grams (7.1 oz) of sliced (washed but unpeeled) potatoes in ~ 1 litre (0.22 imp gal; 0.26 US gal) distilled water for 30 minutes and then
1032: 349: 317: 105:
species. Sabouraud agar is also slightly acid with pH of 5.6-6.0 which is similar to PDA. It is most often used for the isolation of pathogenic fungi such as
1130: 716: 411: 709: 765: 465: 1123: 1149: 264: 251: 357: 1159: 515: 1116: 459: 404: 206:
A similar growth medium, potato dextrose broth (abbreviated "PDB"), is formulated identically to PDA, omitting the
1052: 920: 247: 218: 1057: 1011: 887: 1042: 855: 731: 683: 611: 81:
PDA has the capability to culture various bacteria and fungi found in the soil. This agar can be used with
78:. Potato dextrose agar (abbreviated "PDA") is the most widely used medium for growing fungi and bacteria. 773: 759: 753: 627: 441: 976: 1047: 950: 488: 480: 475: 470: 397: 925: 1037: 1027: 877: 872: 860: 299: 549: 1001: 981: 700: 656: 636: 200: 582: 739: 688: 668: 601: 311: 1154: 1006: 606: 535: 224: 1100: 1071: 996: 882: 587: 212: 195: 521: 494: 85:
or acid to inhibit bacterial/fungal growth. PDA is used in the food industry to test for
303: 986: 971: 940: 851: 779: 595: 450: 98: 42: 1143: 991: 915: 836: 651: 543: 432: 420: 86: 64: 17: 1096: 529: 106: 272: 562: 191: 187: 102: 47: 966: 930: 902: 806: 787: 725: 424: 353: 101:. Malt extract agar is more acidic than PDA and is commonly used to cultivate 90: 82: 389: 892: 865: 745: 677: 506: 32: 1066: 1061: 910: 816: 810: 800: 793: 645: 576: 568: 554: 89:. It is also used in the pharmaceutical industry to screen for potential 71: 36:
Master plate of bacterial soil isolates growing on potato dextrose agar.
1088: 845: 138:(strained broth from 200 g of infused potato into the water above) 75: 68: 210:. Common organisms that can be cultured on PDB are yeasts such as 31: 945: 207: 393: 203:
at 15 pounds per square inch (100 kPa) for 15 minutes.
1104: 1020: 959: 901: 835: 699: 667: 635: 626: 505: 449: 440: 431: 1124: 405: 8: 344: 342: 1131: 1117: 1033:Cystine–lactose–electrolyte-deficient agar 632: 446: 437: 412: 398: 390: 386:, second edition. Lawrence C. Parks (1997) 316:: CS1 maint: location missing publisher ( 298:. Springer Science & Business Media. 243: 241: 263:Harold Eddleman, Ph. D (February 1998). 118: 41: 717:Tryptic soy-serum-bacitracin-vancomycin 237: 309: 710:Aggregatibacter actinomycetemcomitans 7: 1085: 1083: 766:Buffered charcoal yeast extract agar 333:Centraalbureau voor Schimmelcultures 248:BAM Media M127: Potato Dextrose Agar 265:"Making Bacteria Media from Potato" 1103:. You can help Knowledge (XXG) by 87:fungi that can spoil food products 25: 384:Handbook of Microbiological Media 1087: 846:Lactose fermenting gram negative 190:or straining the broth through 52:growing in potato dextrose agar 1: 271:. disknet.com. Archived from 125:ingredients & conditions 252:Food and Drug Administration 516:Corynebacterium diphtheriae 331:"Atlas of clinical fungi". 294:Pitt & Hocking (2009). 63:are common microbiological 1176: 1082: 460:Mycobacterium tuberculosis 1053:Mannitol motility medium 921:Dermatophyte test medium 466:Löwenstein–Jensen medium 219:Saccharomyces cerevisiae 144:(sliced washed unpeeled) 93:agents in medications. 1043:Glucose phosphate broth 856:Sorbitol-MacConkey agar 350:"Potato Dextrose Broth" 296:Fungi and food spoilage 29:Microbial growth medium 1099:-related article is a 774:Pseudomonas aeruginosa 760:Legionella pneumophila 754:Haemophilus influenzae 53: 37: 1150:Microbiological media 1048:Lauryl tryptose broth 1021:Other/ungrouped media 888:Simmons' citrate agar 489:Mycoplasma pneumoniae 481:Middlebrook 7H11 Agar 476:Middlebrook 7H10 Agar 471:Middlebrook 7H9 Broth 61:potato dextrose broth 59:(BAM Media M127) and 45: 35: 18:Potato dextrose broth 1038:Cystine tryptic agar 1028:Brain heart infusion 1012:Zobell's marine agar 936:Potato dextrose agar 878:Hektoen enteric agar 873:Bismuth sulfite agar 861:Eosin methylene blue 57:Potato dextrose agar 1058:Mueller–Hinton agar 1002:Trypticase soy agar 977:Columbia blood agar 701:Gammaproteobacteria 637:Alphaproteobacteria 304:2009ffs..book.....P 113:Typical composition 1160:Microbiology stubs 960:Nonselective media 837:Differential media 740:Enterobacteriaceae 732:Bordet–Gengou agar 689:New York City agar 684:Thayer–Martin agar 669:Betaproteobacteria 612:Vogel–Johnson agar 602:Mannitol salt agar 222:and molds such as 54: 38: 1112: 1111: 1080: 1079: 1007:Tryptic soy broth 926:Malt extract agar 831: 830: 827: 826: 622: 621: 607:Baird-Parker agar 536:Bile esculin agar 225:Aspergillus niger 181: 180: 145: 16:(Redirected from 1167: 1133: 1126: 1119: 1091: 1084: 997:Plate count agar 883:Lysine iron agar 657:Farrell's medium 633: 447: 438: 414: 407: 400: 391: 370: 369: 367: 365: 356:. Archived from 346: 337: 336: 328: 322: 321: 315: 307: 291: 285: 284: 282: 280: 275:on July 16, 2011 260: 254: 245: 213:Candida albicans 143: 119: 21: 1175: 1174: 1170: 1169: 1168: 1166: 1165: 1164: 1140: 1139: 1138: 1137: 1081: 1076: 1016: 955: 897: 823: 695: 663: 618: 501: 433:Selective media 427: 418: 379: 377:Further reading 374: 373: 363: 361: 360:on May 16, 2006 348: 347: 340: 330: 329: 325: 308: 293: 292: 288: 278: 276: 262: 261: 257: 246: 239: 234: 196:Distilled water 142: 115: 30: 23: 22: 15: 12: 11: 5: 1173: 1171: 1163: 1162: 1157: 1152: 1142: 1141: 1136: 1135: 1128: 1121: 1113: 1110: 1109: 1092: 1078: 1077: 1075: 1074: 1069: 1064: 1055: 1050: 1045: 1040: 1035: 1030: 1024: 1022: 1018: 1017: 1015: 1014: 1009: 1004: 999: 994: 989: 987:Lysogeny broth 984: 979: 974: 972:Chocolate agar 969: 963: 961: 957: 956: 954: 953: 948: 943: 941:Sabouraud agar 938: 933: 928: 923: 918: 913: 907: 905: 899: 898: 896: 895: 890: 885: 880: 875: 870: 869: 868: 863: 858: 852:MacConkey agar 841: 839: 833: 832: 829: 828: 825: 824: 822: 821: 820: 819: 803: 798: 797: 796: 784: 783: 782: 780:Cetrimide agar 770: 769: 768: 750: 749: 748: 736: 735: 734: 722: 721: 720: 705: 703: 697: 696: 694: 693: 692: 691: 686: 673: 671: 665: 664: 662: 661: 660: 659: 654: 641: 639: 630: 624: 623: 620: 619: 617: 616: 615: 614: 609: 604: 596:Staphylococcus 592: 591: 590: 585: 573: 572: 571: 559: 558: 557: 552: 540: 539: 538: 526: 525: 524: 511: 509: 503: 502: 500: 499: 498: 497: 485: 484: 483: 478: 473: 468: 455: 453: 451:Actinomycetota 444: 435: 429: 428: 419: 417: 416: 409: 402: 394: 388: 387: 378: 375: 372: 371: 338: 323: 286: 269:Indiana Biolab 255: 250:from the U.S. 236: 235: 233: 230: 183: 182: 179: 178: 175: 171: 170: 167: 163: 162: 159: 155: 154: 151: 147: 146: 139: 135: 134: 131: 127: 126: 123: 114: 111: 99:sabouraud agar 28: 24: 14: 13: 10: 9: 6: 4: 3: 2: 1172: 1161: 1158: 1156: 1153: 1151: 1148: 1147: 1145: 1134: 1129: 1127: 1122: 1120: 1115: 1114: 1108: 1106: 1102: 1098: 1093: 1090: 1086: 1073: 1072:Schädler agar 1070: 1068: 1065: 1063: 1059: 1056: 1054: 1051: 1049: 1046: 1044: 1041: 1039: 1036: 1034: 1031: 1029: 1026: 1025: 1023: 1019: 1013: 1010: 1008: 1005: 1003: 1000: 998: 995: 993: 992:Nutrient agar 990: 988: 985: 983: 982:Letheen broth 980: 978: 975: 973: 970: 968: 965: 964: 962: 958: 952: 949: 947: 944: 942: 939: 937: 934: 932: 929: 927: 924: 922: 919: 917: 916:Czapek medium 914: 912: 909: 908: 906: 904: 900: 894: 891: 889: 886: 884: 881: 879: 876: 874: 871: 867: 864: 862: 859: 857: 853: 850: 849: 848: 847: 843: 842: 840: 838: 834: 818: 815: 814: 813: 812: 808: 804: 802: 799: 795: 792: 791: 790: 789: 785: 781: 778: 777: 776: 775: 771: 767: 764: 763: 762: 761: 756: 755: 751: 747: 744: 743: 742: 741: 737: 733: 730: 729: 728: 727: 723: 719: 718: 714: 713: 712: 711: 707: 706: 704: 702: 698: 690: 687: 685: 682: 681: 680: 679: 675: 674: 672: 670: 666: 658: 655: 653: 652:Brucella agar 650: 649: 648: 647: 643: 642: 640: 638: 634: 631: 629: 628:Gram negative 625: 613: 610: 608: 605: 603: 600: 599: 598: 597: 593: 589: 586: 584: 581: 580: 579: 578: 574: 570: 567: 566: 565: 564: 560: 556: 553: 551: 548: 547: 546: 545: 544:Lactobacillus 541: 537: 534: 533: 532: 531: 527: 523: 520: 519: 518: 517: 513: 512: 510: 508: 504: 496: 493: 492: 491: 490: 486: 482: 479: 477: 474: 472: 469: 467: 464: 463: 462: 461: 457: 456: 454: 452: 448: 445: 443: 442:Gram positive 439: 436: 434: 430: 426: 422: 415: 410: 408: 403: 401: 396: 395: 392: 385: 382:Atlas, R.M.: 381: 380: 376: 359: 355: 351: 345: 343: 339: 334: 327: 324: 319: 313: 305: 301: 297: 290: 287: 274: 270: 266: 259: 256: 253: 249: 244: 242: 238: 231: 229: 227: 226: 221: 220: 215: 214: 209: 204: 202: 197: 193: 189: 176: 173: 172: 168: 165: 164: 160: 157: 156: 152: 149: 148: 140: 137: 136: 132: 129: 128: 124: 121: 120: 117: 116: 112: 110: 108: 107:dermatophytes 104: 100: 94: 92: 88: 84: 79: 77: 73: 70: 66: 62: 58: 51: 49: 44: 40: 34: 27: 19: 1105:expanding it 1097:microbiology 1094: 935: 903:Fungal media 844: 805: 786: 772: 758: 752: 738: 724: 715: 708: 676: 644: 594: 583:Fraser broth 575: 561: 542: 530:Enterococcus 528: 522:Hoyle's agar 514: 495:Eaton's agar 487: 458: 421:Growth media 383: 362:. Retrieved 358:the original 332: 326: 295: 289: 277:. Retrieved 273:the original 268: 258: 223: 217: 211: 205: 184: 177:temperature 161:agar powder 130:1000 mL 95: 80: 65:growth media 60: 56: 55: 46: 39: 26: 563:Lactococcus 425:agar plates 201:autoclaving 192:cheesecloth 103:penicillium 83:antibiotics 48:Aspergillus 1144:Categories 967:Blood agar 931:MMN medium 807:Salmonella 788:Salmonella 726:Bordetella 550:Lee's Agar 354:Merck KGaA 335:(2). 2009. 232:References 91:antifungal 67:made from 893:TSI slant 866:Endo agar 746:VRBD agar 678:Neisseria 507:Bacillota 312:cite book 188:decanting 169:final pH 158:20 g 153:dextrose 150:20 g 1155:Potatoes 1067:R2A agar 1062:PNP agar 911:BAF agar 817:XLD agar 811:Shigella 801:DCA agar 794:XLT agar 646:Brucella 577:Listeria 569:M17 agar 555:MRS agar 279:March 4, 141:potatoes 76:dextrose 72:infusion 364:May 29, 300:Bibcode 166:5.6±0.2 588:PALCAM 133:water 69:potato 1095:This 122:value 1101:stub 946:YEPD 366:2005 318:link 281:2011 216:and 208:agar 174:25°C 74:and 109:. 50:sp. 1146:: 951:YM 423:/ 352:. 341:^ 314:}} 310:{{ 267:. 240:^ 228:. 194:. 1132:e 1125:t 1118:v 1107:. 1060:/ 854:/ 809:/ 757:/ 413:e 406:t 399:v 368:. 320:) 306:. 302:: 283:. 20:)

Index

Potato dextrose broth


Aspergillus
growth media
potato
infusion
dextrose
antibiotics
fungi that can spoil food products
antifungal
sabouraud agar
penicillium
dermatophytes
decanting
cheesecloth
Distilled water
autoclaving
agar
Candida albicans
Saccharomyces cerevisiae
Aspergillus niger


BAM Media M127: Potato Dextrose Agar
Food and Drug Administration
"Making Bacteria Media from Potato"
the original
Bibcode
2009ffs..book.....P

Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.

↑