843:
87:
displaces SSBP from the ssDNA, although SSBP remains attached to RecO. RecFOR then loads RecA onto a recessed 5' end of this ssDNA-dsDNA junction. The RecR subunit in RecFR then interacts with RecO to form the RecFOR complex. In doing so, the RecR subunit helps to both detach the SSBP molecules from RecO and load molecules of the RecA protein onto the 3' overhang.
86:
The RecA protein can be loaded onto the SSBP-coated 3' overhang in one of two distinct pathways, one that requires the RecFOR enzyme or one that requires the RecOR enzyme. In the RecFOR pathway, the RecFR complex binds where the single-strand DNA of the 3' meets the double-strand DNA. RecO then
247:
Kowalczykowski, SC; Clow, J; Somani, R; Varghese, A (5 January 1987). "Effects of the
Escherichia coli SSB protein on the binding of Escherichia coli RecA protein to single-stranded DNA. Demonstration of competitive binding and the lack of a specific protein-protein interaction".
95:
of SSBP. The RecOR pathway also does not need a ssDNA-dsDNA junction to begin loading RecA onto the 3' overhang, whereas the RecFOR pathway typically does to work efficiently. Thus, the RecOR pathway in most conditions is more efficient than the RecFOR pathway in loading RecA.
78:
helicase greatly increase how much the 5' is cut back. When present, SSBP binds to the 3' overhang remaining after RecJ finishes cutting back the 5' strand. By binding to the single-stranded DNA, SSBP ensures that the 3' DNA overhang does not stick to itself through
90:
The RecOR pathway of RecA loading differs from the RecFOR pathway in several respects, most notably its molecular interaction requirements and its ideal DNA substrate. Unlike the RecFOR pathway, the RecOR pathway requires an interaction between RecO and the
43:. It repairs breaks that occur on only one of DNA's two strands, known as single-strand gaps. The RecF pathway can also repair double-strand breaks in DNA when the
70:
direction, binds to the 5' end of a single-strand gap in DNA and starts moving upstream while cleaving the 5' strand. Although RecJ can function without them,
776:
750:
47:, another pathway of homologous recombination in bacteria, is inactivated by mutations. Like the RecBCD pathway, the RecF pathway requires
411:
466:
80:
719:
650:
67:
734:
115:"RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair"
71:
709:
616:
341:"The process of displacing the single-stranded DNA-binding protein from single-stranded DNA by RecO and RecR proteins"
638:
462:
455:
633:
555:
404:
44:
32:
745:
655:
450:
511:
59:
slides in one direction, and resolution, in which the
Holliday junctions are cleaved apart by enzymes.
436:
872:
846:
533:
397:
867:
729:
370:
321:
265:
229:
177:
136:
56:
28:
360:
352:
311:
301:
257:
219:
211:
167:
126:
52:
445:
365:
340:
316:
289:
224:
199:
200:"Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli"
131:
114:
861:
757:
578:
261:
724:
693:
643:
626:
526:
63:
660:
481:
428:
420:
172:
155:
92:
36:
374:
325:
306:
233:
181:
140:
51:
for strand invasion. The two pathways are also similar in their phases of
356:
269:
741:
40:
215:
688:
440:
198:
Handa, N; Morimatsu, K; Lovett, ST; Kowalczykowski, SC (15 May 2009).
813:
568:
521:
516:
339:
Inoue, H; Honda, M; Ikawa, S; Shibata, T; Mikawa, T (January 2008).
828:
823:
818:
808:
801:
796:
791:
786:
781:
771:
766:
621:
585:
506:
501:
496:
486:
714:
670:
665:
600:
595:
590:
563:
543:
538:
491:
75:
48:
393:
476:
471:
156:"DNA Repair: Common Approaches to Fixing Double-Strand Breaks"
389:
290:"RecFOR and RecOR as distinct RecA loading pathways"
702:
681:
609:
554:
427:
113:Morimatsu, K; Kowalczykowski, SC (22 May 2003).
405:
8:
193:
191:
412:
398:
390:
364:
315:
305:
223:
171:
130:
66:that cleaves single-stranded DNA in the
283:
281:
279:
105:
62:The RecF pathway begins when RecJ, an
288:Sakai, A; Cox, MM (30 January 2009).
7:
720:Proliferating Cell Nuclear Antigen
651:Microhomology-mediated end joining
14:
842:
841:
735:Meiotic recombination checkpoint
294:Journal of Biological Chemistry
250:Journal of Biological Chemistry
1:
132:10.1016/S1097-2765(03)00188-6
72:single-strand binding protein
617:Transcription-coupled repair
262:10.1016/0022-2836(87)90629-2
889:
639:Non-homologous end joining
463:Nucleotide excision repair
456:Poly ADP ribose polymerase
837:
173:10.1016/j.cub.2009.06.009
634:Homology directed repair
556:Homologous recombination
33:homologous recombination
204:Genes & Development
656:Postreplication repair
451:Uracil-DNA glycosylase
345:Nucleic Acids Research
307:10.1074/jbc.M807220200
154:Hiom, K (July 2009).
762:core protein complex
437:Base excision repair
534:DNA mismatch repair
357:10.1093/nar/gkm1004
216:10.1101/gad.1780709
23:, also called the
16:DNA repair pathway
855:
854:
730:Adaptive response
210:(10): 1234–1245.
166:(13): R523–R525.
57:Holliday junction
880:
845:
844:
414:
407:
400:
391:
379:
378:
368:
336:
330:
329:
319:
309:
300:(5): 3264–3272.
285:
274:
273:
244:
238:
237:
227:
195:
186:
185:
175:
151:
145:
144:
134:
125:(5): 1337–1347.
110:
53:branch migration
888:
887:
883:
882:
881:
879:
878:
877:
858:
857:
856:
851:
833:
703:Other/ungrouped
698:
677:
605:
550:
446:DNA glycosylase
429:Excision repair
423:
418:
388:
383:
382:
338:
337:
333:
287:
286:
277:
246:
245:
241:
197:
196:
189:
160:Current Biology
153:
152:
148:
112:
111:
107:
102:
81:complementation
74:(SSBP) and the
55:, in which the
17:
12:
11:
5:
886:
884:
876:
875:
870:
860:
859:
853:
852:
850:
849:
838:
835:
834:
832:
831:
826:
821:
816:
811:
806:
805:
804:
799:
794:
789:
784:
779:
774:
769:
754:
753:
748:
738:
737:
732:
727:
722:
717:
712:
706:
704:
700:
699:
697:
696:
691:
685:
683:
679:
678:
676:
675:
674:
673:
668:
658:
653:
648:
647:
646:
636:
631:
630:
629:
624:
613:
611:
610:Other pathways
607:
606:
604:
603:
598:
593:
588:
583:
582:
581:
571:
566:
560:
558:
552:
551:
549:
548:
547:
546:
541:
531:
530:
529:
524:
519:
514:
509:
504:
499:
494:
489:
484:
479:
474:
460:
459:
458:
453:
448:
433:
431:
425:
424:
419:
417:
416:
409:
402:
394:
387:
386:External links
384:
381:
380:
331:
275:
239:
187:
146:
119:Molecular Cell
104:
103:
101:
98:
45:RecBCD pathway
25:RecFOR pathway
15:
13:
10:
9:
6:
4:
3:
2:
885:
874:
871:
869:
866:
865:
863:
848:
840:
839:
836:
830:
827:
825:
822:
820:
817:
815:
812:
810:
807:
803:
800:
798:
795:
793:
790:
788:
785:
783:
780:
778:
775:
773:
770:
768:
765:
764:
763:
759:
758:FANC proteins
756:
755:
752:
749:
747:
743:
740:
739:
736:
733:
731:
728:
726:
723:
721:
718:
716:
713:
711:
708:
707:
705:
701:
695:
692:
690:
687:
686:
684:
680:
672:
669:
667:
664:
663:
662:
659:
657:
654:
652:
649:
645:
642:
641:
640:
637:
635:
632:
628:
625:
623:
620:
619:
618:
615:
614:
612:
608:
602:
599:
597:
594:
592:
589:
587:
584:
580:
579:RecQ helicase
577:
576:
575:
572:
570:
567:
565:
562:
561:
559:
557:
553:
545:
542:
540:
537:
536:
535:
532:
528:
525:
523:
520:
518:
515:
513:
510:
508:
505:
503:
500:
498:
495:
493:
490:
488:
485:
483:
480:
478:
475:
473:
470:
469:
468:
464:
461:
457:
454:
452:
449:
447:
444:
443:
442:
438:
435:
434:
432:
430:
426:
422:
415:
410:
408:
403:
401:
396:
395:
392:
385:
376:
372:
367:
362:
358:
354:
351:(1): 94–109.
350:
346:
342:
335:
332:
327:
323:
318:
313:
308:
303:
299:
295:
291:
284:
282:
280:
276:
271:
267:
263:
259:
255:
251:
243:
240:
235:
231:
226:
221:
217:
213:
209:
205:
201:
194:
192:
188:
183:
179:
174:
169:
165:
161:
157:
150:
147:
142:
138:
133:
128:
124:
120:
116:
109:
106:
99:
97:
94:
88:
84:
82:
77:
73:
69:
65:
60:
58:
54:
50:
46:
42:
38:
34:
30:
26:
22:
761:
742:DNA helicase
725:8-Oxoguanine
694:SOS response
574:RecF pathway
573:
527:Excinuclease
348:
344:
334:
297:
293:
256:(1): 81–95.
253:
249:
242:
207:
203:
163:
159:
149:
122:
118:
108:
89:
85:
61:
24:
21:RecF pathway
20:
18:
64:exonuclease
37:repairs DNA
873:DNA repair
862:Categories
682:Regulation
661:Photolyase
421:DNA repair
100:References
93:C-terminus
502:XPG/ERCC5
487:XPD/ERCC2
868:Genetics
847:Category
497:XPF/DDB1
492:XPE/DDB1
375:18000001
326:18986990
234:19451222
182:19602417
141:12769856
41:bacteria
689:SOS box
441:AP site
366:2248737
317:2631980
270:3295259
225:2685532
29:pathway
27:, is a
814:FANCD2
809:FANCD1
569:RecBCD
522:RAD23B
517:RAD23A
373:
363:
324:
314:
268:
232:
222:
180:
139:
68:5 → 3′
829:FANCN
824:FANCJ
819:FANCI
802:FANCM
797:FANCL
792:FANCG
787:FANCF
782:FANCE
777:FANCC
772:FANCB
767:FANCA
627:ERCC8
622:ERCC6
586:RAD51
507:ERCC1
79:self-
35:that
715:PcrA
671:CRY2
666:CRY1
601:LexA
596:Slx4
591:Sgs1
564:RecA
544:MSH2
539:MLH1
467:ERCC
371:PMID
322:PMID
266:PMID
230:PMID
178:PMID
137:PMID
76:RecQ
49:RecA
19:The
751:WRN
746:BLM
710:Ogt
512:RPA
482:XPC
477:XPB
472:XPA
361:PMC
353:doi
312:PMC
302:doi
298:284
258:doi
254:193
220:PMC
212:doi
168:doi
127:doi
39:in
31:of
864::
760::
744::
644:Ku
369:.
359:.
349:36
347:.
343:.
320:.
310:.
296:.
292:.
278:^
264:.
252:.
228:.
218:.
208:23
206:.
202:.
190:^
176:.
164:19
162:.
158:.
135:.
123:11
121:.
117:.
83:.
465:/
439:/
413:e
406:t
399:v
377:.
355::
328:.
304::
272:.
260::
236:.
214::
184:.
170::
143:.
129::
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.