Knowledge (XXG)

Sudan stain

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146: 740: 220: 234: 192: 178: 206: 749: 164: 116:, a few drops of alcoholic solution of Sudan III are added, the sample is spread on a microscopic slide, and heated twice to boil. Normally a stool sample should show only a few drops of red-orange stained fat under the microscope. The method is only semiquantitative but, due to its simplicity, it is used for 341: 128:
Since they are characteristically oil- and fat-soluble, Sudan dyes are also useful for dyeing plastics and fabrics. Sudan dyes I–IV and Sudan Red G consist of
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Larsen, John Chr. "Legal and illegal colors" Trends in Food Science & Technology (2008), 19(Suppl. 1), S60-S65.
259:, to enhance their colors. This finding has led to controversy because some Sudan dyes are carcinogenic in rats. 1164: 1027: 454: 952: 721: 664: 1238: 716: 490: 1187: 557: 502: 460: 726: 546: 1119: 994: 1056: 1022: 540: 484: 431: 396: 1228: 1223: 1037: 776: 752: 580: 472: 408: 117: 1248: 812: 688: 670: 592: 478: 466: 425: 109: 1154: 643: 631: 598: 219: 648: 626: 1233: 1218: 1171: 1006: 880: 875: 551: 419: 390: 288: 1134: 1032: 744: 604: 414: 308: 280: 233: 39: 27: 1014: 769: 694: 637: 610: 586: 191: 145: 1042: 970: 961: 935: 496: 402: 367: 129: 1212: 1129: 1104: 1072: 956: 947: 931: 855: 682: 621: 616: 225: 205: 177: 67: 761: 1149: 1109: 1092: 867: 705: 700: 163: 79: 1087: 251:
Some spices exported from Asia have been adulterated with Sudan dyes, especially
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Sudan dyes have high affinity to fats, therefore they are used to demonstrate
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are important members of this class of compounds (see images below).
822: 83: 47: 800: 765: 323: 90:. Alcoholic solutions of Sudan dyes are usually used, however 31: 1180: 1065: 1005: 917: 894: 866: 821: 799: 657: 567: 533: 512: 441: 383: 376: 355: 136:. Such compounds are known to exist as a pair of 108:. A small sample is dissolved in water or saline, 94:solutions can be used in some situations as well. 277:Ullmann's Encyclopedia of Industrial Chemistry 275:Booth, Gerald (2000). "Dyes, General Survey". 777: 335: 112:is added to hydrolyze the insoluble salts of 8: 784: 770: 762: 380: 342: 328: 320: 100:is often used to determine the level of 267: 156: 1083:Jaswant Singh–Bhattacharji (JSB) stain 7: 748: 1160:Grocott's methenamine silver stain 14: 793:Microbial and histological stains 747: 738: 232: 218: 204: 190: 176: 162: 144: 1: 350:Analytical reagents and tests 42:) and are also used to stain 543:(aldehyde & ketones etc) 46:biological samples, usually 1265: 886:Periodic acid–Schiff stain 442:Proteins & amino acids 377:Sugars, fats, and proteins 313:10.1016/j.tifs.2008.07.008 735: 1244:IARC Group 3 carcinogens 1028:Light Green SF yellowish 1019:Masson's trichrome stain 978:Auramine–rhodamine stain 722:Fecal coliform detection 487:(aldehyde & ketones) 481:(aldehyde & ketones) 455:Bicinchoninic acid assay 285:10.1002/14356007.a09_073 717:Pesticide detection kit 677:Folin–Ciocalteu reagent 673:(creates free radicals) 491:Van Slyke determination 279:. Weinheim: Wiley-VCH. 1145:Schaeffer–Fulton stain 1115:Gömöri trichrome stain 575:Dille–Koppanyi reagent 558:Ceric ammonium nitrate 503:Xanthoproteic reaction 461:Bradford protein assay 1120:Luxol fast blue stain 995:Auramine phenol stain 665:Dragendorff's reagent 399:(reducing sugars etc) 384:Sugars & starches 1165:Warthin–Starry stain 1038:Phosphomolybdic acid 745:Chemistry portal 613:(MDMA, opiates, etc) 473:Pierce Protein Assay 409:Aniline acetate test 1181:Tissue stainability 953:Ziehl–Neelsen stain 813:Perls Prussian blue 727:Prion detection kit 593:Gallic acid reagent 554:(types of alcohols) 467:Lowry protein assay 158:Selected Sudan dyes 110:glacial acetic acid 1155:Bielschowsky stain 1057:Van Gieson's stain 1023:Lillie's trichrome 644:Salicylate testing 632:Zimmermann reagent 599:Liebermann reagent 577:(barbiturates etc) 541:Benedict's reagent 485:Fehling's solution 432:Fehling's solution 397:Benedict's reagent 1206: 1205: 1007:Connective tissue 759: 758: 634:(Benzodiazepines) 581:Ehrlich's reagent 529: 528: 434:(reducing sugars) 428:(reducing sugars) 393:(Monosaccharides) 40:plastic colorants 30:that are used as 28:organic compounds 1256: 1033:Biebrich scarlet 786: 779: 772: 763: 751: 750: 743: 742: 741: 689:Melzer's reagent 671:Fenton's reagent 605:Mandelin reagent 595:(drug precursor) 547:DenigĂ©s' reagent 479:Tollens' reagent 426:Tollens' reagent 415:Starch indicator 381: 344: 337: 330: 321: 315: 305: 299: 298: 272: 236: 222: 208: 194: 180: 166: 148: 98:Sudan stain test 1264: 1263: 1259: 1258: 1257: 1255: 1254: 1253: 1209: 1208: 1207: 1202: 1176: 1061: 1015:trichrome stain 1001: 913: 890: 862: 817: 795: 790: 760: 755: 739: 737: 731: 695:Marquis reagent 653: 649:Folin's reagent 638:Zwikker reagent 627:Simon's reagent 611:Marquis reagent 587:Froehde reagent 563: 560:test (alcohols) 525: 508: 475:(total protein) 469:(total protein) 463:(concentration) 457:(concentration) 437: 372: 351: 348: 318: 306: 302: 295: 274: 273: 269: 265: 249: 242: 237: 228: 223: 214: 209: 200: 195: 186: 181: 172: 167: 155: 126: 76: 17: 12: 11: 5: 1262: 1260: 1252: 1251: 1246: 1241: 1239:Food colorings 1236: 1231: 1226: 1221: 1211: 1210: 1204: 1203: 1201: 1200: 1195: 1190: 1184: 1182: 1178: 1177: 1175: 1174: 1172:Wright's stain 1169: 1168: 1167: 1162: 1157: 1147: 1142: 1137: 1132: 1127: 1122: 1117: 1112: 1107: 1102: 1101: 1100: 1095: 1085: 1080: 1075: 1069: 1067: 1063: 1062: 1060: 1059: 1053: 1052: 1051: 1050: 1045: 1043:Fast Green FCF 1040: 1035: 1030: 1011: 1009: 1003: 1002: 1000: 999: 998: 997: 992: 991: 990: 985: 975: 974: 973: 971:Methylene blue 968: 962:Carbol fuchsin 945: 944: 943: 938: 936:Gentian violet 923: 921: 915: 914: 912: 911: 906: 900: 898: 892: 891: 889: 888: 883: 878: 872: 870: 864: 863: 861: 860: 859: 858: 853: 848: 843: 838: 827: 825: 819: 818: 816: 815: 809: 807: 797: 796: 791: 789: 788: 781: 774: 766: 757: 756: 736: 733: 732: 730: 729: 724: 719: 714: 708: 703: 698: 692: 686: 685:(caffeine etc) 680: 679:(antioxidants) 674: 668: 661: 659: 655: 654: 652: 651: 646: 641: 640:(barbiturates) 635: 629: 624: 619: 614: 608: 602: 601:(street drugs) 596: 590: 584: 583:(indoles, etc) 578: 571: 569: 565: 564: 562: 561: 555: 552:Lucas' reagent 549: 544: 537: 535: 531: 530: 527: 526: 524: 523: 516: 514: 510: 509: 507: 506: 500: 497:Sakaguchi test 494: 488: 482: 476: 470: 464: 458: 452: 445: 443: 439: 438: 436: 435: 429: 423: 420:Molisch's test 417: 412: 406: 400: 394: 391:Barfoed's test 387: 385: 378: 374: 373: 371: 370: 368:Xylenol orange 365: 359: 357: 353: 352: 349: 347: 346: 339: 332: 324: 317: 316: 300: 293: 266: 264: 261: 248: 245: 244: 243: 238: 231: 229: 224: 217: 215: 210: 203: 201: 196: 189: 187: 182: 175: 173: 168: 161: 159: 154: 151: 150: 149: 125: 122: 75: 72: 26:are synthetic 15: 13: 10: 9: 6: 4: 3: 2: 1261: 1250: 1247: 1245: 1242: 1240: 1237: 1235: 1232: 1230: 1227: 1225: 1222: 1220: 1217: 1216: 1214: 1199: 1196: 1194: 1191: 1189: 1186: 1185: 1183: 1179: 1173: 1170: 1166: 1163: 1161: 1158: 1156: 1153: 1152: 1151: 1148: 1146: 1143: 1141: 1138: 1136: 1135:Movat's stain 1133: 1131: 1130:Moeller stain 1128: 1126: 1123: 1121: 1118: 1116: 1113: 1111: 1108: 1106: 1105:Janus Green B 1103: 1099: 1096: 1094: 1091: 1090: 1089: 1088:H&E stain 1086: 1084: 1081: 1079: 1076: 1074: 1073:Cresyl violet 1071: 1070: 1068: 1064: 1058: 1055: 1054: 1049: 1046: 1044: 1041: 1039: 1036: 1034: 1031: 1029: 1026: 1025: 1024: 1020: 1016: 1013: 1012: 1010: 1008: 1004: 996: 993: 989: 986: 984: 981: 980: 979: 976: 972: 969: 967: 963: 960: 959: 958: 957:Kinyoun stain 954: 951: 950: 949: 946: 942: 939: 937: 933: 932:Methyl violet 930: 929: 928: 925: 924: 922: 920: 916: 910: 907: 905: 902: 901: 899: 897: 893: 887: 884: 882: 879: 877: 874: 873: 871: 869: 868:Carbohydrates 865: 857: 856:Sudan Black B 854: 852: 849: 847: 844: 842: 839: 837: 834: 833: 832: 829: 828: 826: 824: 820: 814: 811: 810: 808: 806: 802: 798: 794: 787: 782: 780: 775: 773: 768: 767: 764: 754: 746: 734: 728: 725: 723: 720: 718: 715: 712: 709: 707: 704: 702: 699: 696: 693: 690: 687: 684: 683:Murexide test 681: 678: 675: 672: 669: 666: 663: 662: 660: 656: 650: 647: 645: 642: 639: 636: 633: 630: 628: 625: 623: 622:Drug checking 620: 618: 617:Mecke reagent 615: 612: 609: 606: 603: 600: 597: 594: 591: 588: 585: 582: 579: 576: 573: 572: 570: 566: 559: 556: 553: 550: 548: 545: 542: 539: 538: 536: 532: 521: 518: 517: 515: 511: 504: 501: 498: 495: 493:(amino acids) 492: 489: 486: 483: 480: 477: 474: 471: 468: 465: 462: 459: 456: 453: 450: 447: 446: 444: 440: 433: 430: 427: 424: 421: 418: 416: 413: 410: 407: 404: 401: 398: 395: 392: 389: 388: 386: 382: 379: 375: 369: 366: 364: 361: 360: 358: 354: 345: 340: 338: 333: 331: 326: 325: 322: 314: 310: 304: 301: 296: 290: 286: 282: 278: 271: 268: 262: 260: 258: 254: 246: 241: 235: 230: 227: 226:Sudan Black B 221: 216: 213: 207: 202: 199: 193: 188: 185: 179: 174: 171: 165: 160: 157: 152: 147: 143: 142: 141: 139: 135: 132:-substituted 131: 123: 121: 119: 115: 111: 107: 103: 99: 95: 93: 89: 85: 81: 80:triglycerides 73: 71: 69: 68:Sudan Black B 65: 61: 57: 53: 49: 45: 41: 37: 33: 29: 25: 21: 16:Group of dyes 1198:Chromophobic 1150:Silver stain 1110:Giemsa stain 1093:Haematoxylin 830: 706:Nitrite test 701:Nitrate test 519: 303: 276: 270: 250: 127: 104:to diagnose 97: 96: 88:lipoproteins 77: 44:sudanophilic 43: 34:for various 23: 20:Sudan stains 19: 18: 1229:2-Naphthols 1224:Stool tests 1188:Acidophilic 1140:Neutral red 1125:Methyl blue 988:Rhodamine B 881:Mucicarmine 876:Alcian blue 831:Sudan stain 805:hemosiderin 667:(alkaloids) 520:Sudan stain 449:Biuret test 403:Bial's test 240:Sudan Red G 114:fatty acids 106:steatorrhea 1249:Sudan dyes 1213:Categories 1193:Basophilic 1048:Sirius Red 983:Auramine O 927:Gram stain 909:Thioflavin 607:(ketamine) 505:(proteins) 499:(arginine) 451:(proteins) 411:(pentoses) 405:(pentoses) 294:3527306730 263:References 24:Sudan dyes 948:Acid-fast 904:Congo red 851:Oil Red O 841:Sudan III 697:(various) 589:(opioids) 257:Sudan III 198:Sudan III 138:tautomers 134:naphthols 118:screening 102:fecal fat 64:Oil Red O 56:Sudan III 1234:Azo dyes 1219:Staining 966:Fuchsine 941:Safranin 919:Bacteria 846:Sudan IV 836:Sudan II 534:Alcohols 522:(lipids) 363:Aluminon 212:Sudan IV 184:Sudan II 153:Examples 92:pyridine 74:Staining 60:Sudan IV 52:Sudan II 36:plastics 1098:Eosin Y 1078:Cyanine 896:Amyloid 753:Commons 713:(blood) 711:Luminol 691:(fungi) 422:(carbs) 253:Sudan I 170:Sudan I 130:arylazo 823:Lipids 356:Metals 291:  247:Safety 124:Dyeing 86:, and 84:lipids 66:, and 48:lipids 1066:Other 658:Other 568:Drugs 801:Iron 513:Fats 289:ISBN 255:and 32:dyes 22:and 309:doi 281:doi 1215:: 1017:: 287:. 140:: 120:. 82:, 62:, 58:, 54:, 50:. 1021:/ 964:/ 955:/ 934:/ 803:/ 785:e 778:t 771:v 343:e 336:t 329:v 311:: 297:. 283:: 38:(

Index

organic compounds
dyes
plastics
plastic colorants
lipids
Sudan II
Sudan III
Sudan IV
Oil Red O
Sudan Black B
triglycerides
lipids
lipoproteins
pyridine
fecal fat
steatorrhea
glacial acetic acid
fatty acids
screening
arylazo
naphthols
tautomers

Sudan I
Sudan I
Sudan II
Sudan II
Sudan III
Sudan III
Sudan IV

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