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links the bait protein to its potential interaction partners. The bait protein together with its potential interaction partners can then be isolated using a Strep-Tactin
Sepharose column. The cross-links between the bait protein and the potential interaction partner can be cleaved by heating the
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samples in
Laemmli buffer. Finally, the co-purified interaction partner can be separated by SDS PAGE and identified by mass spectrometry.
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under the conditions when the potential interaction partners are presumably present in the cells. The addition of
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Membrane-SPINE: an improved method to identify protein–protein interaction partners of membrane proteins in vivo
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Membrane-SPINE: a biochemical tool to identify protein–protein interactions of membrane proteins in vivo
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SPINE: A method for the rapid detection and analysis of protein–protein interactions in vivo
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Herzberg C, Weidinger LA, Dörrbecker B, Hübner S, Stülke J and
Commichau FM (2007).
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in vivo. The bait protein has to be expressed with a
55:MĂĽller VS, Jungblut PR, Meyer TF, Hunke S (2011).
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62:MĂĽller VS, Tschauner K, Hunke S (2013).
23:. SPINE is a powerful tool to detect
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59:. Proteomics 11(10):2124-2128
97:. You can help Knowledge by
52:. Proteomics 7(22):4032-4035
25:protein–protein interactions
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