69:. Malachite green is applied to the slide, which can penetrate the tough walls of the endospores, staining them green. After five minutes, the slide is removed from the steam, and the paper towel is removed. After cooling, the slide is rinsed with water for thirty seconds. The slide is then stained with diluted safranin for two minutes, which stains most other microorganic bodies red or pink. The slide is then rinsed again, and blotted dry with
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Endospores cannot be stained by normal staining procedures because their walls are practically impermeable to all chemicals. The
Schaeffer- Fulton endospore stain uses heat to drive the primary stain(malachite green) into the endospore. After cooling, the slide is decolorized with water and
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by staining any present endospores green, and any other bacterial bodies red. The primary stain is
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The procedure was designed by Alice B. Schaeffer and MacDonald Fulton, two microbiologists at
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Using an aseptic technique, bacteria are placed on a slide and
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showing endospores as green and the vegetative cell as red
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140:Laboratory Exercises in Microbiology
37:is a technique designed to isolate
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125:Definition:Schaeffer-Fulton Stain
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53:counterstained with safranin.
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142:, page 58. McGraw Hill, 2002.
16:Endospore isolation technique
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