90:
sodium channel (which absorbs sodium into the cell). Impaired CFTR functioning directly reduces ductal epithelial chloride secretion and indirectly increases sodium absorption through lack of CFTR's inhibitory effect on the apical sodium channel. The result is dehydrated mucus and a widened, negative
51:
concentrations in sweat, characteristic clinical findings (including sinopulmonary infections), and/or family history. However, a small portion of patients with cystic fibrosis, especially those with "mild" mutations of the
154:
and offers the advantage of continuously monitoring living cells throughout their various stages of growth and differentiation, it is widely accepted as a standard validation tool.
53:
130:
resistance to serve as a proxy for the permeability of a cellular monolayer. TEER therefore enables researchers to miniaturize
71:). Individuals with cystic fibrosis have a significantly more negative nasoepithelial surface than normal, due to increased
146:
systems. TEER has proven to be a highly sensitive and reliable method to confirm the integrity and permeability of
334:
339:
139:
151:
68:
28:
98:
TEPD is increased in cystic fibrosis, making it a potential diagnostic tool for this disorder.
310:
292:
251:
202:
119:
300:
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241:
233:
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184:
123:
83:
72:
44:
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In these cases, a useful diagnostic adjunct involves measuring the nasal transepithelial
305:
270:
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79:
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172:
328:
143:
127:
87:
32:
107:
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Srinivasan B, Kolli AR, Esch MB, Abaci HE, Shuler ML, Hickman JJ (April 2015).
95:
64:
24:
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314:
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48:
60:
20:
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Transepithelial / transendothelial electrical resistance (TEER) is an
135:
131:
222:"TEER measurement techniques for in vitro barrier model systems"
269:
Zidarič, Tanja; Gradišnik, Lidija; Velnar, Tomaž (2022-04-01).
86:, and also has a tonic inhibitory effect on the opening of the
271:"Astrocytes and human artificial blood-brain barrier models"
82:, the CFTR protein normally secretes chloride ions into the
173:"Transepithelial potential difference in cystic fibrosis"
56:(CFTR) ion channel, have near-normal sweat tests.
8:
142:transfer, or membrane integrity assays in
304:
286:
275:Bosnian Journal of Basic Medical Sciences
245:
196:
163:
54:cystic fibrosis transmembrane regulator
91:transepithelial potential difference.
7:
122:technique widely adopted for use in
63:(i.e. the charge on the respiratory
17:Transepithelial potential difference
106:In the kidney, TEPD contributes to
14:
226:Journal of Laboratory Automation
171:Hay JG, Geddes DM (July 1985).
150:barrier models. Because it is
47:(CF) is usually based on high
1:
356:
288:10.17305/bjbms.2021.6943
238:10.1177/2211068214561025
31:for the outer and inner
27:, and is the sum of the
67:surface as compared to
189:10.1136/thx.40.7.493
120:electrophysiological
108:tubular reabsorption
75:sodium absorption.
61:potential difference
140:Blood–brain barrier
29:membrane potentials
102:TEPD in the kidney
69:interstitial fluid
126:systems. It uses
43:The diagnosis of
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335:Membrane biology
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318:
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290:
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114:TEER measurement
39:TEPD in the nose
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340:Cystic fibrosis
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124:organ-on-a-chip
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80:exocrine glands
45:cystic fibrosis
41:
12:
11:
5:
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281:(5): 651–672.
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138:permeability,
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40:
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33:cell membranes
19:(TEPD) is the
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2:
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232:(2): 107–26.
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128:ohmic contact
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183:(7): 493–6.
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152:non-invasive
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144:microfluidic
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42:
16:
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329:Categories
158:References
65:epithelial
25:epithelium
23:across an
297:1840-4812
315:35366791
256:25586998
148:in vitro
134:such as
78:In most
49:chloride
306:9519155
247:4652793
207:4035615
73:luminal
21:voltage
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303:
295:
254:
244:
205:
198:460118
195:
177:Thorax
136:Caco-2
132:assays
88:apical
96:nasal
84:lumen
311:PMID
293:ISSN
252:PMID
203:PMID
94:The
301:PMC
283:doi
242:PMC
234:doi
193:PMC
185:doi
331::
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279:22
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181:40
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187::
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