707:
653:
276:
181:
715:
27:
98:
519:
309:
InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
299:
InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
581:
are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a distinctive blue colour under a microscope. Since live cells are excluded
727:, trypan blue can be used to stain the posterior stromal fibers during deep lamellar endothelial keratoplasty (DLEK) and to stain the endothelium in Descemet's stripping endothelial keratoplasty (DSEK). Trypan blue is used in vitreoretinal surgeries also.
1036:
722:
Trypan blue is also used in ophthalmic cataract surgery to stain the anterior capsule in the presence of a mature cataract, to aid in visualization, before creating the continuous curvilinear capsulorhexis. In
1029:
1022:
457:
1695:
532:
1699:
1691:
1118:
1113:
332:
734:) was inferred, based on the observation that injection of trypan blue in animals led to whole‐body staining except for the brain and spinal cord.
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1176:
1148:
1739:
1734:
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972:
Nowicki, Marcin; et al. (15 May 2013), "A simple dual stain for detailed investigations of plant-fungal pathogen interactions",
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1164:
1724:
1000:
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233:
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662:
340:....S(=O)(=O)c6cc1cc(cc(N)c1c(O)c6/N=N/c2ccc(cc2C)c5ccc(/N=N/c4c(cc3cc(cc(N)c3c4O)S()(=O)=O)S()(=O)=O)c(C)c5)S()(=O)=O
254:
780:
1191:
1559:
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1434:
911:
706:
539:
774:
Chapter "Detection of
Caspase Activation Combined with Other Probes of Apoptosis", Eurekah Bioscience Collection,
652:
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878:
Klaus Hunger; Peter
Mischke; Wolfgang Rieper; Roderich Raue; Klaus Kunde; Aloys Engel (2005). "Azo Dyes".
710:
Vesicular
Arbuscular Mycorrhizae visualised using clearing of tissue followed by staining with Trypan blue
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787:
Wainwright, M. (December 2010). "Dyes, trypanosomiasis and DNA: a historical and critical review".
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In early 20th century, the existence of a barrier protective toward the brain (
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for assessment of tissue viability. The method cannot distinguish between
640:
Trypan red and trypan blue were first synthesized by the German scientist
1201:
1186:
685:
931:
Strober, W (May 2001). "Trypan Blue
Exclusion Test of Cell Viability".
623:
611:
555:
209:
1402:
129:
509:
Except where otherwise noted, data are given for materials in their
51:)-3,3'-bis(5-amino-4-oxo-3,4-dihydronaphthalene-2,7-disulfonic acid)
197:
1001:"Trypan Blue Dye: Capsular Staining for Cataract Surgery and More"
713:
705:
696:
651:
109:
97:
87:
1301:
188:
1018:
861:"Trypan Blue Powder Cell Viability Gold Standard Sigma-Aldrich"
1412:
630:. Trypan blue is also known as diamine blue and Niagara blue.
259:
582:
from staining, this staining method is also described as a
1073:
637:
for trypan blue is 6⋅10 M cm at 607 nm in methanol.
1230:
446:
20 mg/mL in methyl
Cellosolve, and 0.6 mg/mL in ethanol
527:
562:
for cotton textiles. In biosciences, it is used as a
1636:
1598:
1530:
1490:
1443:
1410:
1401:
1228:
1071:
1062:
1445:
1492:
424:> 300 °C (572 °F; 573 K)
221:
1638:
1532:
614:. Trypan blue is so-called because it can kill
72:
1417:Tooltip Glutamine aminohydrolase (glutaminase)
912:"Sigma-Aldrich, 60% Trypan Blue, Product page"
880:Ullmann’s Encyclopedia of Industrial Chemistry
1030:
8:
1600:
598:, that is, any of several isomeric bases, C
1696:Metabotropic glutamate receptor modulators
1407:
1078:Tooltip Excitatory amino acid transporters
1068:
1037:
1023:
1015:
840:"CID 9562061 | C34H24N6Na4O14S4 - PubChem"
274:
179:
157:
18:
985:
241:
1700:GABA metabolism and transport modulators
1692:Ionotropic glutamate receptor modulators
1235:Tooltip Vesicular glutamate transporters
830:
337:
295:
270:
718:Soil arthropod takes trypan blue stain
170:
1272:Amido black 10B (naphthol blue black)
319:Key: GLNADSQYFUSGOU-GPTZEZBUSA-J
302:Key: GLNADSQYFUSGOU-GPTZEZBUSA-J
137:
117:
7:
626:, is used pharmacologically against
467:
312:Key: GLNADSQYFUSGOU-WLILTGDCBQ
212:
196:
1450:Tooltip Aspartate aminotransferase
783:(PDF) from Northwestern University
672:by using the trypan blue staining.
577:Live cells or tissues with intact
14:
1005:American Academy of Ophthalmology
974:Vegetable Crops Research Bulletin
695:It may be used to observe fungal
1497:Tooltip Alanine aminotransferase
676:Trypan blue is commonly used in
517:
373:
367:
25:
1643:Tooltip Glutamate decarboxylase
1537:Tooltip Glutamate dehydrogenase
935:. Appendix 3 (1): Appendix 3B.
933:Current Protocols in Immunology
789:Biotechnic & Histochemistry
513:(at 25 °C , 100 kPa).
1628:Phosphinothricin (glufosinate)
414:deep blue in aqueous solution
391:
385:
379:
361:
1:
1688:Receptor/signaling modulators
1605:Tooltip Glutamine synthetase
941:10.1002/0471142735.ima03bs21
622:. An analog of trypan blue,
594:Trypan blue is derived from
781:Protocol for use of the dye
680:(for cell counting) and in
663:Hyaloperonospora parasitica
618:, the parasites that cause
566:to selectively colour dead
485:or concentration (LD, LC):
1766:
842:. Pubchem.ncbi.nlm.nih.gov
1740:Naphthalenesulfonic acids
1735:IARC Group 2B carcinogens
1681:
1331:Glutamic acid (glutamate)
1297:Aspartic acid (aspartate)
1152:-CCG-III ((2S,3S,4R)-CCG)
1109:Glutamic acid (glutamate)
1091:Aspartic acid (aspartate)
987:10.2478/v10032-012-0016-z
801:10.3109/10520290903297528
507:
481:
476:
450:
348:
328:
286:
56:
38:
33:
24:
1651:3-Mercaptopropionic acid
1160:-Serine-O-sulphate (SOS)
888:10.1002/14356007.a03_245
590:Background and chemistry
77: (tetrasodium salt)
1458:2-Amino-3-butenoic acid
1252:6-(4'-Phenylstyryl)-QDC
1136:-3-Hydroxyaspartic acid
882:. Weinheim: Wiley-VCH.
503:6200 mg/kg (oral, rat)
1623:Methionine sulfoximine
1210:-3-Methylglutamic acid
719:
711:
673:
635:extinction coefficient
1725:Cell culture reagents
717:
709:
655:
1477:-Methylene-aspartate
669:Arabidopsis thaliana
656:Observation with an
584:dye exclusion method
406:872.88
1590:Pyridoxal phosphate
1292:Chicago sky blue 6B
1257:6-Biphenyl-4-yl-QDC
732:blood brain barrier
648:Uses of trypan blue
431:Solubility in water
21:
1613:2-Aminoadipic acid
1482:Hydrazinosuccinate
720:
712:
674:
658:optical microscope
540:Infobox references
19:
1707:
1706:
1677:
1676:
1663:
1397:
1396:
1315:
1312:Erythro-4-methyl-
1246:
1167:
1159:
1151:
1143:
1131:
752:Chlorazol blue 3B
746:Benzamine blue 3B
666:within a leaf of
620:sleeping sickness
548:Chemical compound
546:
545:
255:CompTox Dashboard
99:Interactive image
1757:
1661:
1644:
1640:
1606:
1602:
1538:
1534:
1522:Propargylglycine
1516:
1508:
1498:
1494:
1476:
1451:
1447:
1418:
1414:
1408:
1389:Xanthurenic acid
1313:
1282:Benzopurpurin 4B
1244:
1236:
1232:
1165:
1157:
1149:
1141:
1129:
1079:
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363:
356:Chemical formula
279:
278:
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261:
245:
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121:
101:
76:
29:
22:
16:Blue-colored dye
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1755:
1754:
1710:
1709:
1708:
1703:
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1604:
1594:
1575:Hexachlorophene
1536:
1526:
1514:
1506:
1496:
1486:
1474:
1449:
1439:
1416:
1393:
1356:Reactive blue 2
1234:
1224:
1077:
1058:
1043:
1013:
1012:
1007:. 1 April 2006.
999:
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859:
858:
854:
845:
843:
837:
836:
832:
827:
786:
771:
769:Further reading
764:Niagara blue 3B
758:Dianil blue H3G
755:Diamine blue 3B
743:Azidine blue 3B
740:
682:laboratory mice
650:
628:trypanosomiasis
610:, derived from
609:
605:
601:
592:
549:
542:
537:
536:
535: ?)
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80:
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52:
17:
12:
11:
5:
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1753:
1752:
1750:Naphthylamines
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1358:
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1348:
1343:
1338:
1336:Kynurenic acid
1333:
1328:
1323:
1318:
1316:-glutamic acid
1309:
1307:Direct blue 71
1304:
1299:
1294:
1289:
1284:
1279:
1277:Bafilomycin A1
1274:
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1254:
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950:978-0471142737
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897:978-3527306732
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784:
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776:NCBI bookshelf
770:
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761:Direct blue 14
759:
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739:
736:
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579:cell membranes
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511:standard state
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1723:
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1718:
1717:
1715:
1702:
1701:
1697:
1693:
1689:
1686:
1680:
1670:
1669:Semicarbazide
1667:
1665:
1664:-Allylglycine
1659:
1657:
1654:
1652:
1649:
1648:
1646:
1641:
1635:
1629:
1626:
1624:
1621:
1619:
1616:
1614:
1611:
1610:
1608:
1603:
1597:
1591:
1588:
1586:
1585:Palmitoyl-CoA
1583:
1581:
1580:Hydroxylamine
1578:
1576:
1573:
1571:
1568:
1566:
1563:
1561:
1558:
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1390:
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1221:
1218:
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1213:
1211:
1209:
1205:
1203:
1200:
1198:
1195:
1193:
1190:
1188:
1185:
1183:
1182:Maslinic acid
1180:
1178:
1175:
1173:
1171:
1163:
1161:
1155:
1153:
1147:
1145:
1139:
1137:
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1120:
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1040:
1035:
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1021:
1020:
1017:
1006:
1002:
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993:
988:
983:
979:
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968:
965:
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927:
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913:
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904:
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862:
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834:
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798:
795:(6): 341–54.
794:
790:
785:
782:
779:
777:
773:
772:
768:
763:
760:
757:
754:
751:
749:Benzo Blue bB
748:
745:
742:
741:
737:
735:
733:
728:
726:
716:
708:
704:
702:
701:stramenopiles
698:
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1267:Acid red 114
1243:4-Methylene-
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616:trypanosomes
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57:Identifiers
48:
44:
20:Trypan blue
1745:1-Naphthols
1555:Chloroquine
1384:Valinomycin
1379:Trypan blue
1361:Rose bengal
1346:NPPB (N144)
1220:WAY-213,613
1124:Kainic acid
1086:Amphetamine
1064:Transporter
564:vital stain
552:Trypan blue
497:median dose
483:Lethal dose
411:Appearance
349:Properties
177:100.000.715
119:CHEBI:78897
1714:Categories
1618:JFD01307SC
1351:Ponceau SS
1326:Furosemide
1321:Evans blue
1287:Bumetamide
1247:-glutamate
1056:modulators
1049:metabolism
917:2015-07-15
846:2022-04-19
825:References
678:microscopy
560:direct dye
558:. It is a
442:Solubility
402:Molar mass
243:I2ZWO3LS3M
150:ChemSpider
139:ChEMBL1640
86:3D model (
64:CAS Number
40:IUPAC name
1685:See also:
1550:Bithionol
1505:β-Chloro-
1341:Nigericin
1053:transport
1046:Glutamate
838:PubChem.
690:apoptotic
644:in 1904.
596:toluidine
436:10mg/ml
1720:Azo dyes
1509:-alanine
1215:UCPH-101
1202:Theanine
1197:TFB-TBOA
1187:SYM-2081
1172:-2,4-PDC
959:18432654
817:46525029
809:21080764
738:Synonyms
686:necrotic
477:Hazards
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458:ATC code
159:10482308
692:cells.
624:suramin
612:toluene
568:tissues
556:azo dye
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464:S01KX02
223:5904246
210:PubChem
74:72-57-1
1570:GW5074
1430:CB-839
1403:Enzyme
1231:vGluTs
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697:hyphae
574:blue.
554:is an
528:verify
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333:SMILES
198:C19307
130:ChEMBL
34:Names
1425:BPTES
1374:-ACDP
1372:trans
1262:7-CKA
1208:threo
1170:trans
1134:threo
1132:-(-)-
1119:HIP-B
1114:HIP-A
1099:-ACBD
1074:EAATs
865:Merck
813:S2CID
572:cells
291:InChI
110:ChEBI
88:JSmol
1656:AAOA
1560:EGCG
1545:AAOA
1463:AAOA
1366:SITS
1302:DIDS
1192:TBOA
1177:MPDC
1144:-αAA
1104:DHKA
1051:and
955:PMID
945:ISBN
892:ISBN
805:PMID
699:and
688:and
633:The
234:UNII
189:KEGG
1639:GAD
1565:GTP
1533:GDH
1493:ALT
1468:AMB
1446:AST
1435:DON
1413:GAH
1097:cis
982:doi
937:doi
884:doi
797:doi
660:of
570:or
469:WHO
260:EPA
213:CID
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