31:
294:. The method was subsequently adopted for a variety of other techniques. Specific uses of affinity chromatography include antibody affinity, Immobilized metal ion affinity chromatography and purification of recombinant proteins - possibly the most common use of the method. To purify, proteins are tagged e.g. using
424:
system is a technique for studying the interaction between two biomolecules in an indirect manner, as follows: Biotin is chemically coupled to a binder molecule (e.g., a protein, DNA, hormone, etc.) without disturbing the interaction with its target molecule; avidin is then used to âsandwichâ between
429:
binder and a reporter molecule or probe. This allows for a variety of tasks, including localization and identification of the binder or target molecule. Consequently, the avidin-biotin system can frequently replace radioactive probes. Together with Ed Bayer, Wilchek established the Avidin-biotin
376:. In 1975, they applied drug-conjugated antibodies for the targeted delivery of cytotoxic compounds to cancer cells. They also demonstrated the advantage of having a polymeric spacer between the antibody and the drug and showed the effectiveness of conjugating simple polymers such as
309:
is composed of regulatory and catalytic subunits. In 1972, Wilchek showed that the method can be used to remove toxic compounds from blood, as exemplified by the removal of heme peptides from blood using immobilized human serum albumin, thus laying the grounds for modern
446:
and pull-down assays. More recently, Wilchek participated in structural studies of the avidinâbiotin complex, to characterize the unique properties of this strong interaction. The studies have culminated in the determination of the
1775:
528:
863:
Wilchek, M., Salomon, Y., Lowe, M., and
Selinger, Z. (1971). "Conversion of protein kinase to a cyclic AMP independent form by affinity chromatography on N0-caproyl 3â˛,5â˛-cyclic adenosine monophosphate-Sepharose".
1780:
997:
Hurwitz, E., Levy, R., Maron, R., Wilchek, M., Arnon, R., and Sela, M. (1975). "The covalent binding of daunomycin and adriamycin to antibodies, with retention of both drug and antibody activities".
1810:
1038:
Miron, T., Mironchik, M., Mirelman, D., Wilchek, M., and
Rabinkov, A. (2003). "Inhibition of tumor growth by a novel approach: In situ allicin generation using targeted alliinase delivery".
1805:
948:
Strausbauch, P.H., Weinstein, Y., Wilchek, M., Shaltiel, S., Givol, D. (1971). "A homologous series of affinity labeling reagents and their use in the study of antibody binding sites".
368:
is a biorecognition-based approach to selectively deliver a cytotoxic drug or toxin to a specific target cell. The field of affinity therapy was pioneered by
Wilchek, together with
1815:
1770:
1336:
1246:
1173:
1065:
1024:
983:
899:
772:
717:
668:
434:
antibodies and other biomolecules. Today, the system is applied in research and diagnostics as well as medical devices and pharmaceuticals. Examples include
569:
786:
1785:
488:
1278:
430:
system as a powerful tool in biological sciences. Early in the 1970s, they exploited Avidin as a probe and developed new methods and reagents to
1262:
239:, and was the first to prove experimentally the equation of Forster on dependence of energy transfer on distance, an approach known today as
1329:
240:
344:
residues in order to elucidate the structure of the active site. Using this method, Wilchek collaborated with a team who proved that the
1795:
353:
1790:
1304:
613:
204:
Meir
Wilchek is known for his research in the field of biorecognition or affinity phenomenon, and its various application, e.g. for
169:
1322:
582:
380:
for drug delivery and targeting. This approach was later adopted by others and eventually led to efficient treatment of human
451:
of the avidinâbiotin complex by X-ray crystallography, which aids in the design of specific artificial recognition sites.
177:
145:
129:
78:
1431:
1309:
1581:
172:. He survived, and immigrated to Israel in 1949 with his mother and sister. He graduated with B.Sc. in chemistry from
396:. In 2003, Wilchek collaborated in a team who introduced a system based on antibody-directed enzyme prodrug therapy (
251:
works at the molecular level, thanks to a unique biotechnological procedure for producing large quantities of pure
164:, he escaped from the German-occupied territories to the territories occupied by Russia, and was transferred to
1599:
329:
283:
790:
478:
287:
263:
205:
88:
1800:
1709:
1407:
1346:
470:
1240:
1167:
1059:
1018:
977:
893:
766:
711:
662:
559:
514:
221:
1187:
Domovich-Eisenberg, Y., Pazy, Y., Nir, O., Raboy, B., Bayer, E.A., Wilchek, M., and Livnah, O. (2004).
1765:
1561:
1463:
1423:
1417:
1374:
1200:
1127:
817:
448:
1743:
1692:
1518:
913:
Wilchek, M. (1972). "Purification of the heme peptide of cytochrome c by affinity chromatography".
552:
144:(Hebrew: ×××ר ×׊ר ×××׌'ק, born 17 October 1935) is an Israeli biochemist. He is a professor at the
74:
1259:
1670:
1591:
1512:
1413:
1360:
1079:
Wilchek, M. & Bayer, E.A. (1988). "The avidin-biotin complex in bioanalytical applications".
244:
352:
lies in the Fv portion of the molecule and involves three hypervariable sites, today called the
298:
or GST (glutathione-S-transferase) tags, which can be recognized by a metal ion ligand, such as
1733:
1727:
1595:
1567:
1449:
1437:
1427:
1399:
1382:
1228:
1155:
1096:
1047:
1006:
965:
930:
881:
845:
754:
699:
682:
Edelhoch, H., Brand, L., Wilchek, M. (1967). "Fluorescence studies with tryptophyl peptides".
609:
474:
1686:
1676:
1666:
1508:
1218:
1208:
1145:
1135:
1088:
957:
922:
873:
835:
825:
744:
691:
648:
397:
337:
1713:
1648:
1642:
1636:
1632:
1575:
1473:
1314:
1266:
1204:
1131:
821:
1680:
1585:
1571:
1496:
1490:
1443:
1378:
321:
306:
209:
1223:
1188:
840:
805:
749:
732:
1759:
1656:
1557:
1502:
1459:
1150:
1115:
1092:
926:
877:
431:
426:
381:
311:
1739:
1723:
1662:
1626:
1609:
1605:
1530:
1524:
1368:
1364:
1291:
Editor, ĂGV. (2015). Wilhelm Exner Medal. Austrian Trade
Association. ĂGV. Austria.
504:
435:
369:
365:
345:
225:
102:
1189:"Structural elements responsible for conversion of streptavidin to a pseudoenzyme"
603:
1719:
1652:
1540:
1469:
393:
341:
267:
270:
mixtures, based on a highly specific biologic interaction such as that between
1534:
389:
373:
349:
119:
98:
168:, while his father, who served as a community rabbi in Warsaw, was killed in
1403:
1213:
1140:
405:
401:
385:
299:
161:
1232:
1051:
1159:
1100:
969:
934:
885:
849:
830:
758:
703:
180:. Wilchek has published over 400 scientific papers, and consulted various
30:
1010:
637:"Conversion of the L-serine residue to an L-cysteine residue in peptides"
463:
1981 Honorary Member of the
American Society of Biological Chemistry
325:
275:
236:
173:
961:
787:"Therapeutic Effects of Garlic Clarified by Weizmann Institute Research"
695:
653:
636:
545:
443:
377:
295:
271:
252:
193:
181:
165:
1116:"Three-dimensional structures of avidin and the avidin-biotin complex"
733:"The circular dichroism of tyrosyl and tryptophanyl diketopiperazines"
421:
417:
333:
291:
279:
248:
232:
217:
213:
189:
185:
157:
54:
247:. More recently, he participated in a research team who studied how
439:
305:
In 1971, Wilchek and colleagues applied this method to show that
243:. He also studied the fine structure of these chromophores using
789:. Weizmann Institute of Science. 14 October 1997. Archived from
541:
2000 Honorary
Doctorate, Ben-Gurion University of the Negev
1318:
1114:
Livnah, O., Bayer, E.A., Wilchek, M., and
Sussman, J. (1993).
527:
1776:
Israel Prize in life sciences recipients who were biochemists
1279:"Israel Prize Official Site - Recipients in 1990 (in Hebrew)"
500:
1989 Barnett
Lecturer, Northeastern University, Boston
806:"Selective enzyme purification by affinity chromatography"
1781:
Members of the Israel Academy of Sciences and Humanities
565:
2004 Wilhelm-Exner Medal, OGV, President of Austria
635:
Zioudrou, C., Wilchek, M., and Patchornik, A. (1965).
1811:
Members of the European Academy of Sciences and Arts
484:
1987 Pierce Prize for Biorecognition Technology
408:
agent, allicin, in situ (at the site) of the cancer
1702:
1619:
1550:
1483:
1392:
1353:
160:, Poland, scion of a rabbinical family. During the
125:
115:
94:
84:
70:
62:
37:
21:
731:Edelhoch, H., Lippoldt, R.E., Wilchek, M. (1968).
220:system. The avidin-biotin complex is the highest
1806:Academic staff of Weizmann Institute of Science
255:, garlic's main biologically active component.
804:Cuatrecasas P, Wilchek M, Anfinsen CB (1968).
529:International Federation of Clinical Chemistry
224:interaction in nature, and its utilization to
176:university and Ph.D. in biochemistry from the
1330:
510:1990 Sarstedt Prize (Numbrecht, Germany)
481:and its applications to biomedical sciences."
384:by recombinant humanized anti-HER2 antibody (
328:that is similar in structure to a particular
8:
1245:: CS1 maint: multiple names: authors list (
1172:: CS1 maint: multiple names: authors list (
1064:: CS1 maint: multiple names: authors list (
1023:: CS1 maint: multiple names: authors list (
982:: CS1 maint: multiple names: authors list (
898:: CS1 maint: multiple names: authors list (
771:: CS1 maint: multiple names: authors list (
716:: CS1 maint: multiple names: authors list (
667:: CS1 maint: multiple names: authors list (
184:companies. He was also in the party list of
1816:Members of the National Academy of Medicine
1310:The Wolf Prize in Medicine in 1987 (detail)
572:, presented by the Prime Minister of Israel
1337:
1323:
1315:
231:Other contributions include conversion of
18:
1222:
1212:
1149:
1139:
839:
829:
748:
652:
489:Israel Academy of Sciences and Humanities
228:integrates all of the former approaches.
1771:Israel Prize in life sciences recipients
477:, "for the invention and development of
594:
466:1984 Rothschild Prize in Chemistry
460:1981â1982 Fogarty International Scholar
1238:
1165:
1057:
1016:
975:
891:
764:
709:
660:
1305:2 Researchers Share Prize in Medicine
7:
544:2002 Honorary Citizen, City of
513:1993 Foreign Associate Member,
340:. These molecules covalently modify
336:. It is considered to be a class of
354:complementarity-determining regions
517:, National Academy of Science, USA
14:
605:Who's Who in the World: 1991-1992
1786:Wolf Prize in Medicine laureates
497:, University of Waterloo, Canada
487:1988 Elected Member of the
29:
583:List of Israel Prize recipients
538:, University Jyvaskyla, Finland
170:FlossenbĂźrg concentration camp
16:Israeli biochemist (born 1935)
1:
866:Biochem. Biophys. Res. Commun
750:10.1016/S0021-9258(18)93189-3
534:2000 Doctor of Science,
524:, Bar Ilan University, Israel
520:1995 Doctor of Science,
493:1989 Doctor of Science,
400:), using antibody-conjugated
178:Weizmann Institute of Science
146:Weizmann Institute of Science
130:Weizmann Institute of Science
79:Weizmann Institute of Science
1093:10.1016/0003-2697(88)90120-0
927:10.1016/0003-2697(72)90464-2
878:10.1016/0006-291X(71)90142-2
212:, affinity therapy, and the
560:Christian B. Anfinsen Award
1832:
1796:Polish emigrants to Israel
1265:February 26, 2009, at the
1260:The Wolf Prize in Medicine
810:Proc. Natl. Acad. Sci. USA
266:is a method of separating
156:Meir Wilchek was born in
135:
108:
28:
1791:20th-century Polish Jews
412:The avidin-biotin system
200:Scientific contributions
152:Early life and education
1214:10.1073/pnas.0308541101
1141:10.1073/pnas.90.11.5076
479:affinity chromatography
338:irreversible inhibitors
264:Affinity chromatography
259:Affinity chromatography
206:affinity chromatography
89:Affinity chromatography
1724:Lynne Elizabeth Maquat
1710:Emmanuelle Charpentier
1347:Wolf Prize in Medicine
562:of The Protein Society
471:Wolf Prize in Medicine
1193:Proc. Natl. Acad. Sci
1120:Proc. Natl. Acad. Sci
831:10.1073/pnas.61.2.636
608:. Marquis Who's Who.
515:Institute of Medicine
372:, Ester Hurwitz, and
364:Affinity therapy, or
1562:Alexander Varshavsky
1464:Elizabeth F. Neufeld
1424:Jean-Pierre Changeux
1418:Stanley Norman Cohen
388:) in a mixture with
1693:Jeffrey M. Friedman
1519:Stanley B. Prusiner
1205:2004PNAS..101.5916E
1132:1993PNAS...90.5076L
962:10.1021/bi00799a029
822:1968PNAS...61..636C
793:on 8 November 2005.
696:10.1021/bi00854a024
654:10.1021/bi00885a018
553:Wilhelm Exner Medal
75:Bar Ilan University
1671:Jeffrey V. Ravetch
1592:Alexander Levitzki
1513:Yasutomi Nishizuka
1414:Barbara McClintock
602:who, M.W. (1990).
507:, in life sciences
245:circular dichroism
1753:
1752:
1734:Daniel J. Drucker
1596:Anthony R. Hunter
1568:Ralph L. Brinster
1450:Pedro Cuatrecasas
1438:Donald F. Steiner
1428:Solomon H. Snyder
1383:Oleh Hornykiewicz
1345:Laureates of the
1199:(16): 5916â5921.
1126:(11): 5076â5080.
1046:(12): 1295â1301.
956:(13): 2631â2638.
743:(18): 4799â4805.
475:Pedro Cuatrecasas
455:Honors and awards
317:Affinity labeling
210:affinity labeling
139:
138:
110:Scientific career
1823:
1744:JosĂŠ-Alain Sahel
1687:James P. Allison
1677:Lewis C. Cantley
1667:Philippa Marrack
1509:Michael Berridge
1339:
1332:
1325:
1316:
1292:
1289:
1283:
1282:
1275:
1269:
1257:
1251:
1250:
1244:
1236:
1226:
1216:
1184:
1178:
1177:
1171:
1163:
1153:
1143:
1111:
1105:
1104:
1076:
1070:
1069:
1063:
1055:
1040:Mol. Cancer Ther
1035:
1029:
1028:
1022:
1014:
1005:(5): 1175â1181.
994:
988:
987:
981:
973:
945:
939:
938:
910:
904:
903:
897:
889:
872:(5): 1177â1184.
860:
854:
853:
843:
833:
801:
795:
794:
783:
777:
776:
770:
762:
752:
728:
722:
721:
715:
707:
679:
673:
672:
666:
658:
656:
647:(9): 1811â1822.
632:
626:
625:
623:
622:
599:
360:Affinity therapy
51:
47:
45:
33:
19:
1831:
1830:
1826:
1825:
1824:
1822:
1821:
1820:
1756:
1755:
1754:
1749:
1714:Jennifer Doudna
1698:
1649:Nahum Sonenberg
1643:Ronald M. Evans
1637:Rudolf Jaenisch
1633:Shinya Yamanaka
1615:
1582:Robert Weinberg
1576:Oliver Smithies
1546:
1479:
1474:Edward B. Lewis
1408:James L. Gowans
1388:
1369:Jon J. van Rood
1349:
1343:
1301:
1296:
1295:
1290:
1286:
1277:
1276:
1272:
1267:Wayback Machine
1258:
1254:
1237:
1186:
1185:
1181:
1164:
1113:
1112:
1108:
1078:
1077:
1073:
1056:
1037:
1036:
1032:
1015:
996:
995:
991:
974:
947:
946:
942:
912:
911:
907:
890:
862:
861:
857:
803:
802:
798:
785:
784:
780:
763:
730:
729:
725:
708:
681:
680:
676:
659:
634:
633:
629:
620:
618:
616:
601:
600:
596:
591:
579:
473:, jointly with
457:
414:
362:
332:for a specific
319:
261:
202:
154:
71:Alma mater
58:
52:
49:
48:17 October 1935
43:
41:
24:
17:
12:
11:
5:
1829:
1827:
1819:
1818:
1813:
1808:
1803:
1798:
1793:
1788:
1783:
1778:
1773:
1768:
1758:
1757:
1751:
1750:
1748:
1747:
1737:
1731:
1728:Adrian Krainer
1717:
1706:
1704:
1700:
1699:
1697:
1696:
1690:
1684:
1681:C. Ronald Kahn
1674:
1660:
1646:
1640:
1630:
1623:
1621:
1617:
1616:
1614:
1613:
1603:
1589:
1586:Roger Y. Tsien
1579:
1572:Mario Capecchi
1565:
1554:
1552:
1548:
1547:
1545:
1544:
1538:
1528:
1522:
1516:
1506:
1500:
1497:Seymour Benzer
1494:
1491:Maclyn McCarty
1487:
1485:
1481:
1480:
1478:
1477:
1467:
1457:
1447:
1444:Osamu Hayaishi
1441:
1435:
1432:James W. Black
1421:
1411:
1400:CĂŠsar Milstein
1396:
1394:
1390:
1389:
1387:
1386:
1379:Arvid Carlsson
1372:
1357:
1355:
1351:
1350:
1344:
1342:
1341:
1334:
1327:
1319:
1313:
1312:
1307:
1300:
1299:External links
1297:
1294:
1293:
1284:
1270:
1252:
1179:
1106:
1071:
1030:
989:
940:
921:(2): 572â575.
905:
855:
796:
778:
723:
690:(2): 547â559.
674:
627:
614:
593:
592:
590:
587:
586:
585:
578:
575:
574:
573:
566:
563:
556:
549:
542:
539:
532:
525:
518:
511:
508:
501:
498:
491:
485:
482:
467:
464:
461:
456:
453:
413:
410:
361:
358:
322:Affinity label
318:
315:
307:protein kinase
260:
257:
201:
198:
153:
150:
137:
136:
133:
132:
127:
123:
122:
117:
113:
112:
106:
105:
96:
92:
91:
86:
85:Known for
82:
81:
72:
68:
67:
64:
60:
59:
53:
39:
35:
34:
26:
25:
22:
15:
13:
10:
9:
6:
4:
3:
2:
1828:
1817:
1814:
1812:
1809:
1807:
1804:
1802:
1801:Living people
1799:
1797:
1794:
1792:
1789:
1787:
1784:
1782:
1779:
1777:
1774:
1772:
1769:
1767:
1764:
1763:
1761:
1745:
1741:
1738:
1735:
1732:
1729:
1725:
1721:
1718:
1715:
1711:
1708:
1707:
1705:
1701:
1694:
1691:
1688:
1685:
1682:
1678:
1675:
1672:
1668:
1664:
1661:
1658:
1657:Victor Ambros
1654:
1650:
1647:
1644:
1641:
1638:
1634:
1631:
1628:
1625:
1624:
1622:
1618:
1611:
1607:
1604:
1601:
1597:
1593:
1590:
1587:
1583:
1580:
1577:
1573:
1569:
1566:
1563:
1559:
1558:Avram Hershko
1556:
1555:
1553:
1549:
1542:
1539:
1536:
1532:
1529:
1526:
1523:
1520:
1517:
1514:
1510:
1507:
1504:
1503:Judah Folkman
1501:
1498:
1495:
1492:
1489:
1488:
1486:
1482:
1475:
1471:
1468:
1465:
1461:
1460:Henri G. Hers
1458:
1455:
1451:
1448:
1445:
1442:
1439:
1436:
1433:
1429:
1425:
1422:
1419:
1415:
1412:
1409:
1405:
1401:
1398:
1397:
1395:
1391:
1384:
1380:
1376:
1373:
1370:
1366:
1362:
1359:
1358:
1356:
1352:
1348:
1340:
1335:
1333:
1328:
1326:
1321:
1320:
1317:
1311:
1308:
1306:
1303:
1302:
1298:
1288:
1285:
1280:
1274:
1271:
1268:
1264:
1261:
1256:
1253:
1248:
1242:
1234:
1230:
1225:
1220:
1215:
1210:
1206:
1202:
1198:
1194:
1190:
1183:
1180:
1175:
1169:
1161:
1157:
1152:
1147:
1142:
1137:
1133:
1129:
1125:
1121:
1117:
1110:
1107:
1102:
1098:
1094:
1090:
1086:
1082:
1081:Anal. Biochem
1075:
1072:
1067:
1061:
1053:
1049:
1045:
1041:
1034:
1031:
1026:
1020:
1012:
1008:
1004:
1000:
993:
990:
985:
979:
971:
967:
963:
959:
955:
951:
944:
941:
936:
932:
928:
924:
920:
916:
915:Anal. Biochem
909:
906:
901:
895:
887:
883:
879:
875:
871:
867:
859:
856:
851:
847:
842:
837:
832:
827:
823:
819:
816:(2): 636â43.
815:
811:
807:
800:
797:
792:
788:
782:
779:
774:
768:
760:
756:
751:
746:
742:
738:
737:J. Biol. Chem
734:
727:
724:
719:
713:
705:
701:
697:
693:
689:
685:
678:
675:
670:
664:
655:
650:
646:
642:
638:
631:
628:
617:
615:9780837911106
611:
607:
606:
598:
595:
588:
584:
581:
580:
576:
571:
567:
564:
561:
557:
554:
550:
547:
543:
540:
537:
536:honoris causa
533:
530:
526:
523:
522:honoris causa
519:
516:
512:
509:
506:
502:
499:
496:
495:honoris causa
492:
490:
486:
483:
480:
476:
472:
468:
465:
462:
459:
458:
454:
452:
450:
445:
441:
437:
433:
428:
423:
419:
411:
409:
407:
404:to produce a
403:
399:
395:
391:
387:
383:
382:breast cancer
379:
375:
371:
367:
359:
357:
355:
351:
347:
343:
339:
335:
331:
327:
323:
316:
314:
313:
312:hemoperfusion
308:
303:
301:
297:
293:
289:
285:
281:
277:
273:
269:
265:
258:
256:
254:
250:
246:
242:
238:
234:
229:
227:
223:
219:
215:
211:
207:
199:
197:
195:
191:
187:
183:
179:
175:
171:
167:
163:
159:
151:
149:
147:
143:
134:
131:
128:
124:
121:
118:
114:
111:
107:
104:
100:
97:
93:
90:
87:
83:
80:
76:
73:
69:
65:
61:
56:
50:(age 88)
40:
36:
32:
27:
20:
1740:Botond Roska
1663:John Kappler
1627:Axel Ullrich
1610:Aharon Razin
1606:Howard Cedar
1531:Michael Sela
1525:Mary F. Lyon
1454:Meir Wilchek
1453:
1375:Roger Sperry
1365:Jean Dausset
1361:George Snell
1287:
1273:
1255:
1241:cite journal
1196:
1192:
1182:
1168:cite journal
1123:
1119:
1109:
1084:
1080:
1074:
1060:cite journal
1043:
1039:
1033:
1019:cite journal
1002:
998:
992:
978:cite journal
953:
950:Biochemistry
949:
943:
918:
914:
908:
894:cite journal
869:
865:
858:
813:
809:
799:
791:the original
781:
767:cite journal
740:
736:
726:
712:cite journal
687:
684:Biochemistry
683:
677:
663:cite journal
644:
641:Biochemistry
640:
630:
619:. Retrieved
604:
597:
535:
521:
505:Israel Prize
494:
449:3D structure
436:western blot
427:biotinylated
415:
370:Michael Sela
366:immunotoxins
363:
346:binding site
320:
304:
262:
230:
226:biochemistry
203:
155:
142:Meir Wilchek
141:
140:
126:Institutions
109:
103:Israel Prize
23:Meir Wilchek
1766:1935 births
1720:Joan Steitz
1653:Gary Ruvkun
1600:Tony Pawson
1541:Eric Kandel
1470:John Gurdon
1087:(1): 1â32.
432:biotinylate
394:doxorubicin
342:active site
268:biochemical
63:Nationality
1760:Categories
1535:Ruth Arnon
999:Cancer Res
621:2014-12-13
589:References
570:Emet Prize
568:2005
558:2004
551:2004
503:1990
469:1987
390:paclitaxel
374:Ruth Arnon
350:antibodies
120:Biochemist
99:Wolf Prize
44:1935-10-17
1404:Leo Sachs
406:cytotoxic
402:alliinase
386:Herceptin
330:substrate
300:imidazole
284:substrate
237:cysteines
162:Holocaust
1578:(2002/3)
1527:(1996/7)
1521:(1995/6)
1515:(1994/5)
1440:(1984/5)
1263:Archived
1233:15079055
1052:14707270
577:See also
548:, Israel
356:(CDRs).
326:molecule
296:His-tags
288:receptor
276:antibody
222:affinity
192:for the
174:Bar Ilan
77:and the
57:, Poland
1201:Bibcode
1160:8506353
1128:Bibcode
1101:3044183
970:5105033
935:4343271
886:4332593
850:4971842
818:Bibcode
759:5687722
704:6047638
546:Rehovot
444:ELISPOT
378:dextran
272:antigen
253:allicin
233:serines
194:Knesset
182:biotech
166:Siberia
66:Israeli
1746:(2024)
1736:(2023)
1730:(2021)
1716:(2020)
1695:(2019)
1689:(2017)
1683:(2016)
1673:(2015)
1659:(2014)
1645:(2012)
1639:(2011)
1629:(2010)
1612:(2008)
1602:(2005)
1588:(2004)
1564:(2001)
1543:(1999)
1537:(1998)
1505:(1992)
1499:(1991)
1493:(1990)
1476:(1989)
1466:(1988)
1456:(1987)
1446:(1986)
1434:(1982)
1420:(1981)
1410:(1980)
1385:(1979)
1371:(1978)
1231:
1224:395898
1221:
1158:
1148:
1099:
1050:
1011:164279
1009:
968:
933:
884:
848:
841:225207
838:
757:
702:
612:
531:(IFCC)
422:biotin
418:avidin
334:enzyme
292:ligand
280:enzyme
249:garlic
218:biotin
214:avidin
190:Meimad
186:Mafdal
158:Warsaw
116:Fields
95:Awards
55:Warsaw
1703:2020s
1620:2010s
1551:2000s
1484:1990s
1393:1980s
1354:1970s
1151:46657
440:ELISA
398:ADEPT
324:is a
286:, or
1247:link
1229:PMID
1174:link
1156:PMID
1097:PMID
1066:link
1048:PMID
1025:link
1007:PMID
984:link
966:PMID
931:PMID
900:link
882:PMID
846:PMID
773:link
755:PMID
718:link
700:PMID
669:link
610:ISBN
425:the
416:The
392:and
290:and
282:and
274:and
241:FRET
188:and
38:Born
1219:PMC
1209:doi
1197:101
1146:PMC
1136:doi
1089:doi
1085:171
958:doi
923:doi
874:doi
836:PMC
826:doi
745:doi
741:243
692:doi
649:doi
348:of
235:to
1762::
1742:/
1726:/
1722:/
1712:/
1679:/
1669:/
1665:/
1655:/
1651:/
1635:/
1608:/
1598:/
1594:/
1584:/
1574:/
1570:/
1560:/
1533:/
1511:/
1472:/
1462:/
1452:/
1430:/
1426:/
1416:/
1406:/
1402:/
1381:/
1377:/
1367:/
1363:/
1243:}}
1239:{{
1227:.
1217:.
1207:.
1195:.
1191:.
1170:}}
1166:{{
1154:.
1144:.
1134:.
1124:90
1122:.
1118:.
1095:.
1083:.
1062:}}
1058:{{
1042:.
1021:}}
1017:{{
1003:35
1001:.
980:}}
976:{{
964:.
954:10
952:.
929:.
919:49
917:.
896:}}
892:{{
880:.
870:45
868:.
844:.
834:.
824:.
814:61
812:.
808:.
769:}}
765:{{
753:.
739:.
735:.
714:}}
710:{{
698:.
686:.
665:}}
661:{{
643:.
639:.
442:,
438:,
302:.
278:,
208:,
196:.
148:.
101:,
46:)
1338:e
1331:t
1324:v
1281:.
1249:)
1235:.
1211::
1203::
1176:)
1162:.
1138::
1130::
1103:.
1091::
1068:)
1054:.
1044:2
1027:)
1013:.
986:)
972:.
960::
937:.
925::
902:)
888:.
876::
852:.
828::
820::
775:)
761:.
747::
720:)
706:.
694::
688:6
671:)
657:.
651::
645:4
624:.
555:.
420:â
216:-
42:(
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
