201:. Self-replicating minicircles hold great promise for the systematic modification of stem cells and will significantly extend the potential of their plasmidal precursor forms ("parental plasmids"), the more as the principal feasibility of such an approach has amply been demonstrated for their plasmidal precursor forms.
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Minicircle preparation from a parental plasmid. The parental plasmid contains two recombinase target sites (black half arrows). Recombination between these sites generates the desired minicircle (bottom right) together with the miniplasmid (bottom left). The hook on the red minicircle-insert stands
191:, so they do not replicate within the target cells and the encoded genes will disappear as the cell divides (which can be either an advantage or disadvantage depending on whether the application demands persistent or transient expression). A novel addition to the field are nonviral
588:
Nehlsen, Kristina; Broll, Sandra; Kandimalla, Raju; Heinz, Niels; Heine, Markus; Binius, Stefanie; Schambach, Axel; Bode, Jürgen (5 April 2013). "Replicating
Minicircles: Overcoming the Limitations of Transient and Stable Expression Systems". In Schleef, Martin (ed.).
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sequences, they are less likely to be perceived as foreign and destroyed. (Typical transgene delivery methods involve plasmids, which contain foreign DNA.) The smaller size of minicircles also extends their
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Argyros, O., Wong SP., Fedonidis C.; et al. (2011). "Development of S/MAR minicircles for enhanced and persistent transgene expression in the mouse liver".
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Nehlsen, K., Broll S., Bode, J. (2006). "Replicating minicircles: Generation of nonviral episomes for the efficient modification of dividing cells".
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Broll, S., Oumard A., Hahn K., Schambach A, Bode, J. . (2010). "Minicircle
Performance Depending on S/MAR-Nuclear Matrix Interactions".
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recovery of the resulting minicircle (vehicle for the highly efficient modification of the recipient cell) and the miniplasmid by
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Dorrell, Richard G.; Nisbet, R. Ellen R.; Barbrook, Adrian C.; Rowden, Stephen J.L.; Howe, Christopher J. (2019).
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Heinz, N, Broll S, Schleef M, Baum C, Bode J (2012). "Filling a gap: S/MAR-based replicating minicircles".
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Minicircle and
Miniplasmid DNA Vectors: The Future of Nonviral and Viral Gene Transfer
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at the end of this process but still in bacteria. These steps are followed by the
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of mammalian cells, with the advantage that, since they contain no bacterial
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Barbrook, Adrian C.; Voolstra, Christian R.; Howe, Christopher J. (2014).
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The purified minicircle can be transferred into the recipient cell by
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genome is made of minicircles that encode chloroplast proteins.
257:"The Chloroplast Genome of a Symbiodinium sp. Clade C3 Isolate"
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production of a 'parental plasmid' (bacterial plasmid with
593:. Wiley‐VCH Verlag GmbH & Co. KGaA. pp. 115–162.
392:"A robust system for production of minicircle DNA vectors"
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minicircles, which owe this property to the presence of a
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Their preparation usually follows a two-step procedure:
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Pages displaying short descriptions of redirect targets
235:"Kinetoplastids and Their Networks of Interlocked DNA"
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capacity and facilitates their delivery into cells.
180:and into a differentiated tissue by, for instance,
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155:excision of prokaryotic vector parts via two
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159:-target sequences at both ends of the insert
105:derivatives that have been freed from all
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390:Kay, M.A., He, C.-Y, Chen, Z.-H. (2010).
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35:for a scaffold-matrix attachment region (
27:Small, circular replicating units of DNA
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101:Minicircles are small (~4kb) circular
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97:experimentally-derived minicircles
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187:Conventional minicircles lack an
112:parts. They have been applied as
54:. They occur naturally in some
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164:capillary gel electrophoresis
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274:10.1016/j.protis.2013.09.006
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150:site-specific recombinase
68:-derived kinetoplast of
213: – Type of plasmid
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189:origin of replication
72:, minicircles encode
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396:Nature Biotechnology
359:Gene Ther. Mol. Biol
310:Amphidinium carterae
118:genetic modification
629:Molecular genetics
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402:(12): 1287–1289.
116:carriers for the
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238:. Retrieved
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174:transfection
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140:inserts) in
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70:trypanosomes
66:mitochondria
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504:J. Mol. Med
267:(1): 1–13.
178:lipofection
157:recombinase
107:prokaryotic
88:chloroplast
83:Amphidinium
78:RNA editing
50:) circular
44:Minicircles
18:Minicircles
623:Categories
563:: 271–277.
365:: 233–244.
221:References
138:eukaryotic
74:guide RNAs
56:eukaryotic
344:198240765
114:transgene
64:. In the
59:organelle
52:replicons
532:23986907
524:21301798
475:20004666
426:21102455
336:31415953
312:Plastid"
293:24316380
211:Episomes
205:See also
95:In vitro
417:4144359
316:Protist
261:Protist
143:E. coli
127:cloning
103:plasmid
62:genomes
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110:vector
86:, the
528:S2CID
340:S2CID
166:(CGE)
80:. In
603:ISBN
575:link
546:link
520:PMID
489:link
471:PMID
440:link
422:PMID
377:link
332:PMID
289:PMID
242:2019
76:for
595:doi
512:doi
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459:395
412:PMC
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324:doi
320:170
279:hdl
269:doi
265:165
176:or
122:DNA
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