1157:
204:
associated with cancers. They are transferred over to these genetic disease models by electroporation/transformation, transfection with a suitable virus or microinjection. BACs can also be utilized to detect genes or large sequences of interest and then used to map them onto the human chromosome
225:
have been cloned as BACs. These constructs are referred to as "infectious clones", as transfection of the BAC construct into host cells is sufficient to initiate viral infection. The infectious property of these BACs has made the study of many viruses such as the
238:
more accessible. Molecular studies of these viruses can now be achieved using genetic approaches to mutate the BAC while it resides in bacteria. Such genetic approaches rely on either linear or circular targeting vectors to carry out
65:. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division. The bacterial artificial chromosome's usual insert size is 150–350
209:. BACs are preferred for these kind of genetic studies because they accommodate much larger sequences without the risk of rearrangement, and are therefore more stable than other types of cloning vectors.
196:
sequences that will govern a gene's expression level. BACs have been used to some degree of success with mice when studying neurological diseases such as
Alzheimer's disease or as in the case of
448:"Construction of a 750-kb bacterial clone contig and restriction map in the region of human chromosome 21 containing the progressive myoclonus epilepsy gene"
192:
mice. BACs have been useful in this field as complex genes may have several regulatory sequences upstream of the encoding sequence, including various
752:
540:"Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells"
1186:
102:, resulting in the genomic sequence of the organism. BACs were replaced with faster and less laborious sequencing methods like whole genome
838:
1125:
74:
446:
Stone NE, Fan JB, Willour V, Pennacchio LA, Warrington JA, Hu A, de la
Chapelle A, Lehesjoki AE, Cox DR, Myers RM (March 1996).
345:"Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector"
1027:
913:
1181:
1140:
1135:
1120:
745:
277:
267:
713:
107:
718:
48:
185:
724:
240:
92:, though they have been replaced by more modern technologies. In BAC sequencing, short piece of the organism's
138:
for partitioning F plasmid DNA to daughter cells during division and ensures stable maintenance of the BAC.
1160:
853:
738:
257:
200:
associated with Down syndrome. There have also been instances when they have been used to study specific
1037:
881:
148:
1072:
1055:
886:
610:
551:
356:
309:
272:
89:
1067:
1050:
908:
791:
167:
96:
is amplified as an insert in BACs, and then sequenced. Finally, the sequenced parts are rearranged
599:"Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome"
961:
956:
831:
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103:
17:
1045:
1007:
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903:
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871:
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510:
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374:
364:
317:
61:
489:"Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome"
995:
966:
487:
Almazán F, González JM, Pénzes Z, Izeta A, Calvo E, Plana-Durán J, Enjuanes L (May 2000).
614:
555:
404:"The development and applications of the bacterial artificial chromosome cloning system"
360:
343:
Shizuya H, Birren B, Kim UJ, Mancino V, Slepak T, Tachiiri Y, Simon M (September 1992).
313:
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132:
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36:
597:
Messerle M, Crnkovic I, Hammerschmidt W, Ziegler H, Koszinowski UH (December 1997).
1105:
925:
843:
762:
300:, Bender W (June 1989). "Construction of large DNA segments in Escherichia coli".
1017:
297:
222:
218:
603:
Proceedings of the
National Academy of Sciences of the United States of America
544:
Proceedings of the
National Academy of Sciences of the United States of America
493:
Proceedings of the
National Academy of Sciences of the United States of America
349:
Proceedings of the
National Academy of Sciences of the United States of America
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81:
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321:
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52:
40:
423:
1115:
1110:
1060:
262:
252:
918:
152:
734:
709:
658:"Epstein-Barr virus genetics: talking about the BAC generation"
980:
898:
863:
818:
801:
93:
77:
has also been produced from the DNA of P1 bacteriophage.
656:
Feederle R, Bartlett EJ, Delecluse HJ (December 2010).
721:(company that sells BAC clones from genomic libraries)
124:
for plasmid replication and regulation of copy number.
1086:
1036:
979:
934:
862:
817:
800:
769:
184:BACs are now being utilized to a greater extent in
710:The Big Bad BAC: Bacterial Artificial Chromosomes
746:
8:
814:
753:
739:
731:
727:(company that makes custom BAC libraries)
683:
673:
632:
622:
573:
563:
514:
504:
463:
422:
378:
368:
402:Shizuya H, Kouros-Mehr H (March 2001).
288:
7:
170:for transcription of inserted genes.
39:, based on a functional fertility
25:
877:Micro
538:Domi A, Moss B (September 2002).
175:Contribution to models of disease
1156:
1155:
832:precursor, heterogenous nuclear
29:bacterial artificial chromosome
18:Bacterial Artificial Chromosome
962:Trans-acting small interfering
926:Enhancer RNAs
844:Transfer
1:
849:Ribosomal
827:Messenger
217:The genomes of several large
1187:Molecular biology techniques
411:The Keio Journal of Medicine
84:the genomes of organisms in
278:Yeast artificial chromosome
268:Human artificial chromosome
1203:
1028:Multicopy single-stranded
872:Interferential
714:Science Creative Quarterly
186:modelling genetic diseases
1151:
942:Guide
904:Small nuclear
624:10.1073/pnas.94.26.14759
241:homologous recombination
155:at the cloning site for
80:BACs were often used to
1018:Genomic
506:10.1073/pnas.97.10.5516
370:10.1073/pnas.89.18.8794
322:10.1126/science.2660262
1121:Artificial chromosomes
909:Small nucleolar
565:10.1073/pnas.192420599
258:End-sequence profiling
151:; some BACs also have
114:Common gene components
106:and now more recently
914:Small Cajal Body RNAs
675:10.1186/2042-4280-1-6
149:antibiotic resistance
967:Subgenomic messenger
882:Small interfering
854:Transfer-messenger
712:— a review from the
273:Secondary chromosome
157:blue/white selection
90:Human Genome Project
1182:Genomics techniques
615:1997PNAS...9414759M
556:2002PNAS...9912415D
361:1992PNAS...89.8794S
314:1989Sci...244.1307O
108:next-gen sequencing
996:Chloroplast
839:modified Messenger
802:Ribonucleic acids
465:10.1101/gr.6.3.218
213:Infectious disease
188:, often alongside
104:shotgun sequencing
88:, for example the
1169:
1168:
1046:Xeno
1008:Complementary
981:Deoxyribonucleic
975:
974:
952:Small hairpin
424:10.2302/kjm.50.26
308:(4910): 1307–12.
180:Inherited disease
143:selectable marker
16:(Redirected from
1194:
1159:
1158:
1136:Yeast
957:Small temporal
887:Piwi-interacting
815:
811:
792:Deoxynucleotides
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748:
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732:
725:Amplicon Express
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609:(26): 14759–63.
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550:(19): 12415–20.
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1088:Cloning vectors
1082:
1068:Locked
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719:Empire Genomics
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499:(10): 5516–21.
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452:Genome Research
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168:phage promoters
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86:genome projects
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763:nucleic acids
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704:External links
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355:(18): 8794–7.
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71:cloning vector
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1056:Threose
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1001:Mitochondrial
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819:Translational
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662:Herpesviridae
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37:DNA construct
34:
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19:
1131:Bacterial
1130:
1106:Lambda phage
770:Constituents
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417:(1): 26–30.
414:
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305:
301:
296:O'Connor M,
291:
216:
183:
163:T7 & Sp6
162:
127:
119:
97:
79:
69:. A similar
60:
49:transforming
47:), used for
32:
28:
26:
1126:P1-derived
894:Antisense
787:Nucleotides
782:Nucleosides
777:Nucleobases
223:RNA viruses
219:DNA viruses
1176:Categories
1078:Morpholino
991:Organellar
899:Processual
864:Regulatory
808:non-coding
284:References
232:poxviruses
205:using BAC
198:aneuploidy
190:transgenic
59:, usually
1038:Analogues
1023:Hachimoji
806:(coding,
761:Types of
202:oncogenes
99:in silico
73:called a
45:F-plasmid
1161:Category
1096:Phagemid
947:Ribozyme
694:21429237
668:(1): 6.
584:12196634
525:10805807
433:11296661
298:Peifer M
247:See also
194:promoter
82:sequence
57:bacteria
1101:Plasmid
685:3063228
643:9405686
611:Bibcode
552:Bibcode
474:8963899
389:1528894
357:Bibcode
330:2660262
310:Bibcode
302:Science
62:E. coli
53:cloning
41:plasmid
35:) is a
1116:Fosmid
1111:Cosmid
1061:Hexose
983:acids
935:Others
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575:129459
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263:Fosmid
253:Cosmid
207:arrays
1141:Human
919:Y RNA
634:25110
516:25860
407:(PDF)
380:50007
690:PMID
639:PMID
580:PMID
521:PMID
470:PMID
429:PMID
385:PMID
326:PMID
234:and
221:and
153:lacZ
147:for
133:parB
131:and
129:parA
120:repE
51:and
43:(or
680:PMC
670:doi
629:PMC
619:doi
570:PMC
560:doi
511:PMC
501:doi
460:doi
419:doi
375:PMC
365:doi
318:doi
306:244
94:DNA
75:PAC
67:kbp
55:in
33:BAC
1178::
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31:(
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