424:(UV) methods there is no visible color change but the principle is exactly the same, i.e. the measurement of a change in the absorbance of the solution. UV methods usually measure the difference in absorbance at 340 nm wavelength between
507:
Greenan, N. S., R.L. Mulvaney, and G.K. Sims. 1995. "A microscale method for colorimetric determination of urea in soil extracts".
425:
682:
672:
333:
677:
569:
Heidari-Bafroui, Hojat; Ribeiro, Brenno; Charbaji, Amer; Anagnostopoulos, Constantine; Faghri, Mohammad (2020-10-16).
205:
at a specified temperature (usually 37°C) to provide the optimum conditions for the enzymes to act. Examples follow.
198:
59:
386:
In this case, only the first stage of the reaction is catalyzed by an enzyme. The second stage is non-enzymatic.
83:
71:
315:
450:
327:
323:
35:
227:
600:
369:
186:
169:
55:
47:
571:"Portable infrared lightbox for improving the detection limits of paper-based phosphate devices"
592:
534:
231:
31:
86:. Recently, colorimetric analyses developed for colorimeters have been adapted for use with
16:
Method of determining concentration of a chemical species in a solution using a color reagent
645:
582:
489:
43:
27:
319:
309:
266:
202:
521:
405:
340:
237:
201:, more accurate results can be obtained. Enzymatic analysis is always carried out in a
587:
570:
666:
604:
297:
173:
87:
39:
23:
58:
and for industrial purposes, e.g. the analysis of water samples in connection with
379:
344:
79:
78:
and a suitable color reagent. The process may be automated, e.g. by the use of an
467:
454:
421:
219:
483:
472:
348:
281:
245:
135:
596:
270:
190:
165:
51:
446:
404:
PAP = phenol + aminophenazone (in some methods the phenol is replaced by
305:
301:
258:
139:
109:
75:
375:
365:
241:
223:
194:
122:
288:
In this case, both stages of the reaction are catalyzed by enzymes.
476:
429:
262:
361:
277:
152:
480:
336:) --> dihydroxyacetone phosphate + hydrogen peroxide
155:+ bathophenanthroline disulfonate → colored complex
90:to speed up analysis and reduce the waste stream.
626:Rey and Wielinger (1970) Z. analyt. chem. 252:224
112:+ o-cresolphthalein complexone → colored complex
189:) the color reaction is preceded by a reaction
185:In enzymatic analysis (which is widely used in
197:. As the enzyme is specific to a particular
8:
125:+ bathocuproin disulfonate → colored complex
586:
332:Glycerol-3-phosphate + oxygen --(enzyme
500:
550:, Churchill, London, 1947, pp 839-844
54:stage. The method is widely used in
7:
50:and may be used with or without an
401:GPO = glycerol-3-phosphate oxidase
14:
588:10.1016/j.measurement.2020.108607
548:Practical Physiological Chemistry
426:nicotinamide adenine dinucleotide
486:in water with a bluing reaction.
248:)--> colored complex + water
22:is a method of determining the
520:Ray Sarkar and Chauhan (1967)
292:Triglycerides (GPO-PAP method)
1:
509:Commun. Soil Sci. Plant Anal.
214:Cholesterol (CHOD-PAP method)
334:glycerol-3-phosphate oxidase
70:The equipment required is a
42:. It is applicable to both
699:
546:Hawk, Oser and Summerson,
395:CHOD = cholesterol oxidase
253:Glucose (GOD-Perid method)
60:industrial water treatment
644:Fawcett and Scott (1960)
382:----> colored complex
683:Absorption spectroscopy
351:)--> colored complex
284:)--> colored complex
230:)--> cholestenone +
84:flow injection analysis
408:, which is less toxic)
339:Hydrogen peroxide + 4-
236:Hydrogen peroxide + 4-
176:→ blue colored complex
451:lactate dehydrogenase
398:GOD = glucose oxidase
374:Ammonium carbonate +
160:Phosphate (inorganic)
94:Non-enzymatic methods
20:Colorimetric analysis
673:Analytical chemistry
324:glycerol-3-phosphate
276:Hydrogen peroxide +
187:medical laboratories
56:medical laboratories
657:Reference to follow
635:Reference to follow
617:Reference to follow
559:Reference to follow
416:Ultraviolet methods
273:+ hydrogen peroxide
228:cholesterol oxidase
48:inorganic compounds
678:Chemical reactions
370:ammonium carbonate
364:+ water --(enzyme
300:+ water --(enzyme
170:ammonium molybdate
38:with the aid of a
535:Clin. Chim. Acta.
449:+ NADH --(enzyme
232:hydrogen peroxide
181:Enzymatic methods
142:→ colored complex
44:organic compounds
32:chemical compound
690:
658:
655:
649:
646:J. Clin. Pathol.
642:
636:
633:
627:
624:
618:
615:
609:
608:
590:
566:
560:
557:
551:
544:
538:
531:
525:
518:
512:
511:26:2519-2529.
505:
490:Nessler cylinder
411:POD = peroxidase
28:chemical element
698:
697:
693:
692:
691:
689:
688:
687:
663:
662:
661:
656:
652:
643:
639:
634:
630:
625:
621:
616:
612:
568:
567:
563:
558:
554:
545:
541:
533:Zak, B. (1958)
532:
528:
519:
515:
506:
502:
498:
479:that indicates
464:
443:
438:
418:
392:
358:
320:glycerol kinase
310:carboxylic acid
294:
267:glucose oxidase
255:
216:
211:
203:buffer solution
183:
162:
149:
132:
119:
106:
101:
96:
68:
17:
12:
11:
5:
696:
694:
686:
685:
680:
675:
665:
664:
660:
659:
650:
637:
628:
619:
610:
561:
552:
539:
526:
522:Anal. Biochem.
513:
499:
497:
494:
493:
492:
487:
470:
463:
460:
459:
458:
442:
439:
437:
434:
428:(NAD) and its
417:
414:
413:
412:
409:
406:4-chlorophenol
402:
399:
396:
391:
388:
384:
383:
372:
357:
354:
353:
352:
341:aminophenazone
337:
330:
312:
293:
290:
286:
285:
274:
254:
251:
250:
249:
238:aminophenazone
234:
215:
212:
210:
207:
182:
179:
178:
177:
161:
158:
157:
156:
148:
145:
144:
143:
131:
128:
127:
126:
118:
115:
114:
113:
105:
102:
100:
97:
95:
92:
67:
64:
15:
13:
10:
9:
6:
4:
3:
2:
695:
684:
681:
679:
676:
674:
671:
670:
668:
654:
651:
647:
641:
638:
632:
629:
623:
620:
614:
611:
606:
602:
598:
594:
589:
584:
580:
576:
572:
565:
562:
556:
553:
549:
543:
540:
536:
530:
527:
523:
517:
514:
510:
504:
501:
495:
491:
488:
485:
482:
478:
474:
471:
469:
466:
465:
461:
456:
452:
448:
445:
444:
440:
435:
433:
432:form (NADH).
431:
427:
423:
415:
410:
407:
403:
400:
397:
394:
393:
390:Abbreviations
389:
387:
381:
377:
373:
371:
367:
363:
360:
359:
355:
350:
346:
342:
338:
335:
331:
329:
325:
321:
317:
313:
311:
307:
303:
299:
298:Triglycerides
296:
295:
291:
289:
283:
279:
275:
272:
268:
264:
260:
257:
256:
252:
247:
243:
239:
235:
233:
229:
225:
221:
218:
217:
213:
208:
206:
204:
200:
196:
192:
188:
180:
175:
174:ascorbic acid
171:
167:
164:
163:
159:
154:
151:
150:
146:
141:
137:
134:
133:
129:
124:
121:
120:
116:
111:
108:
107:
103:
98:
93:
91:
89:
88:plate readers
85:
81:
77:
73:
65:
63:
61:
57:
53:
49:
45:
41:
40:color reagent
37:
33:
29:
25:
24:concentration
21:
653:
640:
631:
622:
613:
578:
574:
564:
555:
547:
542:
529:
516:
508:
503:
419:
385:
380:hypochlorite
345:chlorophenol
287:
184:
80:AutoAnalyzer
69:
19:
18:
575:Measurement
484:surfactants
468:Blood sugar
422:ultraviolet
314:Glycerol +
261:+ oxygen +
220:Cholesterol
72:colorimeter
667:Categories
581:: 108607.
496:References
473:MBAS assay
349:peroxidase
347:--(enzyme
318:--(enzyme
282:peroxidase
280:--(enzyme
265:--(enzyme
246:peroxidase
244:--(enzyme
226:--(enzyme
136:Creatinine
130:Creatinine
605:225140011
597:0263-2241
455:L-lactate
271:gluconate
199:substrate
191:catalyzed
166:Phosphate
66:Equipment
52:enzymatic
462:See also
453:)-->
447:Pyruvate
441:Pyruvate
436:Examples
368:)-->
322:)-->
306:glycerol
304:)-->
302:esterase
269:)-->
209:Examples
99:Examples
76:cuvettes
36:solution
481:anionic
430:reduced
259:Glucose
140:picrate
110:Calcium
104:Calcium
74:, some
648:13:156
603:
595:
524:20:155
376:phenol
366:urease
242:phenol
224:oxygen
195:enzyme
193:by an
123:Copper
117:Copper
82:or by
601:S2CID
537:3:328
477:assay
475:, an
457:+ NAD
263:water
34:in a
26:of a
593:ISSN
362:Urea
356:Urea
343:+ 4-
278:ABTS
153:Iron
147:Iron
46:and
583:doi
579:173
420:In
328:ADP
316:ATP
30:or
669::
599:.
591:.
577:.
573:.
378:+
326:+
308:+
240:+
222:+
172:+
168:+
138:+
62:.
607:.
585::
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