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light purple by complexation of cobalt with the barbiturate nitrogens. The test, in a slightly different formulation, was developed in the 1930s by the
Hungarian-American pharmacologist Theodore Koppanyi (1901–1985) and the American Biochemist, James Madison Dille (1928–1986).
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Koppanyi, T.; Dille, J. M.; Murphy, W. S.; Krop, S. (1934). "Studies on barbiturates. II. Contributions to methods of barbital research".
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O’Neal, C. L.; Crouch, D. J.; Fatah, A. A. (2000). "Validation of twelve chemical spot tests for the detection of drugs of abuse".
40:(v/v) in methanol. Two drops of A are dropped onto the substance followed by one drop of B and any change in colour is observed.
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Dille, J. M.; Koppanyi, T. (1934). "Studies on barbiturates. III. Chemical assay of barbiturates".
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Law
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is used as a simple spot-test to presumptively identify
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36:. Part B made up of is 5%
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57:secobarbital
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152:. July 2000
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311:Categories
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91:References
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