170:
24:
321:
377:
dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels. The high photon yield of fura-2 allowed the first real time (video rate) measurements of calcium inside living cells in 1986. More recently, genetically encoded calcium indicators based on spectral variants of the
376:
and 380 nm of light, and the ratio of the emissions at those wavelengths is directly related to the amount of intracellular calcium. Regardless of the presence of calcium, Fura-2 emits at 510 nm of light. The use of the ratio automatically cancels out confounding variables, such as variable
193:
InChI=1S/C29H27N3O14/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42)
203:
InChI=1/C29H27N3O14/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42)
71:
334:
584:
219:
464:
Cannell, MB; Berlin, JR; Lederer, WJ (1987). "Intracellular calcium in cardiac myocytes: calcium transients measured using fluorescence imaging".
421:
184:
491:
Tsien, Roger Y.; Miyawaki, Atsushi; Llopis, Juan; Heim, Roger; McCaffery, J. Michael; Adams, Joseph A.; Ikura, Mitsuhiko (1997).
559:
554:
341:
594:
589:
127:
148:
385:, have supplemented the use of Fura-2 and other small molecule dyes for calcium imaging, but Fura-2 remains faster.
574:
569:
378:
357:
599:
564:
504:
165:
37:
530:
382:
604:
579:
522:
473:
446:
512:
436:
242:
136:
91:
47:
369:
508:
169:
312:
441:
548:
534:
422:"A new generation of Ca2+ indicators with greatly improved fluorescence properties*"
116:
493:"Fluorescent indicators for Cabased on green fluorescent proteins and calmodulin"
227:
O=C(O)CN(c4ccc(cc4OCCOc3cc1c(oc(c1)c2ncc(o2)C(=O)O)cc3N(CC(=O)O)CC(=O)O)C)CC(=O)O
23:
361:
300:
82:
373:
526:
477:
450:
394:
365:
103:
400:
311:
Except where otherwise noted, data are given for materials in their
517:
492:
70:
60:
153:
372:, and remains very popular. Fura-2 is excited at 340
329:
420:
Grynkiewicz, G.; Poenie, M. & Tsien, R.Y. (1985).
115:
46:
8:
397:, a membrane-permeant derivative of Fura-2
168:
90:
15:
516:
440:
135:
368:. It was the first widely used dye for
466:Society of General Physiologists Series
412:
224:
189:
164:
364:dye which binds to free intracellular
585:Benzofuran ethers at the benzene ring
196:Key: YFHXZQPUBCBNIP-UHFFFAOYSA-N
7:
206:Key: YFHXZQPUBCBNIP-UHFFFAOYAO
106:
14:
319:
22:
315:(at 25 °C , 100 kPa).
1:
442:10.1016/S0021-9258(19)83641-4
621:
305:831.99 g/mol
379:green fluorescent protein
309:
235:
215:
180:
30:
21:
358:aminopolycarboxylic acid
560:Cell culture reagents
555:Biochemistry methods
595:Hydroquinone ethers
590:Potassium compounds
509:1997Natur.388..882M
360:, is a ratiometric
18:
342:Infobox references
16:
350:Chemical compound
348:
347:
149:CompTox Dashboard
72:Interactive image
612:
575:Fluorescent dyes
570:Chelating agents
539:
538:
520:
488:
482:
481:
461:
455:
454:
444:
435:(6): 3440–3450.
426:
417:
332:
326:
323:
322:
294:
293:
292:
284:
283:
275:
274:
266:
265:
257:
256:
243:Chemical formula
173:
172:
157:
155:
139:
119:
108:
94:
74:
50:
26:
19:
620:
619:
615:
614:
613:
611:
610:
609:
545:
544:
543:
542:
503:(6645): 882–7.
490:
489:
485:
463:
462:
458:
424:
419:
418:
414:
409:
391:
370:calcium imaging
351:
344:
339:
338:
337: ?)
328:
324:
320:
316:
291:
288:
287:
286:
282:
279:
278:
277:
273:
270:
269:
268:
264:
261:
260:
259:
255:
252:
251:
250:
248:
245:
231:
228:
223:
222:
211:
208:
207:
204:
198:
197:
194:
188:
187:
176:
158:
151:
142:
122:
109:
97:
77:
64:
53:
40:
12:
11:
5:
618:
616:
608:
607:
602:
597:
592:
587:
582:
577:
572:
567:
562:
557:
547:
546:
541:
540:
483:
456:
411:
410:
408:
405:
404:
403:
398:
390:
387:
349:
346:
345:
340:
318:
317:
313:standard state
310:
307:
306:
303:
297:
296:
289:
280:
271:
262:
253:
246:
241:
238:
237:
233:
232:
230:
229:
226:
218:
217:
216:
213:
212:
210:
209:
205:
202:
201:
199:
195:
192:
191:
183:
182:
181:
178:
177:
175:
174:
166:DTXSID20242203
161:
159:
147:
144:
143:
141:
140:
132:
130:
124:
123:
121:
120:
112:
110:
102:
99:
98:
96:
95:
87:
85:
79:
78:
76:
75:
67:
65:
58:
55:
54:
52:
51:
43:
41:
36:
33:
32:
28:
27:
13:
10:
9:
6:
4:
3:
2:
617:
606:
603:
601:
600:Glycol ethers
598:
596:
593:
591:
588:
586:
583:
581:
578:
576:
573:
571:
568:
566:
563:
561:
558:
556:
553:
552:
550:
536:
532:
528:
524:
519:
518:10.1038/42264
514:
510:
506:
502:
498:
494:
487:
484:
479:
475:
471:
467:
460:
457:
452:
448:
443:
438:
434:
430:
429:J. Biol. Chem
423:
416:
413:
406:
402:
399:
396:
393:
392:
388:
386:
384:
380:
375:
371:
367:
363:
359:
355:
343:
336:
331:
314:
308:
304:
302:
299:
298:
247:
244:
240:
239:
234:
225:
221:
214:
200:
190:
186:
179:
171:
167:
163:
162:
160:
150:
146:
145:
138:
134:
133:
131:
129:
126:
125:
118:
114:
113:
111:
105:
101:
100:
93:
89:
88:
86:
84:
81:
80:
73:
69:
68:
66:
62:
57:
56:
49:
45:
44:
42:
39:
35:
34:
29:
25:
20:
565:Cell imaging
500:
496:
486:
469:
465:
459:
432:
428:
415:
353:
352:
31:Identifiers
362:fluorescent
236:Properties
549:Categories
472:: 201–14.
407:References
381:, such as
301:Molar mass
137:TSN3DL106G
83:ChemSpider
59:3D model (
48:96314-98-6
38:CAS Number
383:Cameleons
605:Anilines
580:Oxazoles
535:13745050
395:Fura-2AM
389:See also
527:9278050
505:Bibcode
478:3505361
451:3838314
366:calcium
335:what is
333: (
295:
104:PubChem
17:Fura-2
533:
525:
497:Nature
476:
449:
401:Indo-1
354:Fura-2
330:verify
327:
220:SMILES
531:S2CID
425:(PDF)
356:, an
185:InChI
117:57054
92:51442
61:JSmol
523:PMID
474:PMID
447:PMID
128:UNII
513:doi
501:388
437:doi
433:260
154:EPA
107:CID
551::
529:.
521:.
511:.
499:.
495:.
470:42
468:.
445:.
431:.
427:.
374:nm
281:14
263:22
254:29
537:.
515::
507::
480:.
453:.
439::
325:Y
290:5
285:K
276:O
272:3
267:N
258:H
249:C
156:)
152:(
63:)
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
