29:
881:
861:
415:
is poisonous and present in plants endemic to
Australia, Africa, and Central America, livestock are often killed by fluoroacetate poisoning. Fluoroacetate is lethal to sheep and cattle at doses of 0.25 to 0.5 mg/kg of body weight, and is a problem in the livestock industry. A fluoroacetate
287:
395:
atom, moving the halogen atom. This action is common to haloalkane dehalogenase and 4-chlorobenzoyl-CoA dehalogenase. DL-2-Haloacid dehalogenase is unique in that a
199:
218:
922:
365:
946:
440:
at sufficiently high levels to detoxify fluoroacetate in the surrounding medium. Scientists and farmers want to determine a way to get
580:
211:
28:
138:
736:
162:
851:
915:
380:
Haloacetate dehalogenase is unique because it catalyzes the cleavage of the remarkably stable carbon–fluorine bond of a
461:
369:
34:
721:
837:
824:
811:
798:
785:
772:
759:
731:
387:. In the reaction of L-2-haloacid dehalogenase and fluoroacetate dehalogenase, the carboxylate group performs a
951:
685:
628:
546:
MacKenzie, D. Trouble in the wind over altered soya beans. New
Scientist. Vol 148 (2006),pp12. December 2, 1995
294:
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156:
49:
908:
633:
143:
272:
654:
573:
223:
726:
131:
66:
511:"Carbon-fluorine bond cleavage. II. Studies on the mechanism of the defluorination of fluoroacetate"
690:
310:
61:
159:
623:
453:
384:
298:
83:
880:
555:
Tatsuo
Kurihara Journal of Biochemistry. Vol. 131, pp. 671–677 (2002), Regular paper;2002
941:
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457:
412:
322:
259:
150:
892:
669:
664:
638:
566:
522:
487:
396:
340:, one of the largest known enzyme families comprising approximately 1% of the genes in the
716:
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613:
349:
119:
552:
Plant biotech will hit farming sector radar screen in 1996. BioBusiness. December 8, 1995
95:
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832:
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236:
888:
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430:
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345:
337:
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255:
501:
536:
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348:, and acts specifically on halide bonds in carbon-halide compounds. The
330:
400:
247:
126:
107:
819:
589:
476:"The Enzymatic Cleavage of the Carbon-Fluorine Bond in Fluoroacetate"
318:
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206:
102:
90:
78:
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427:
302:
543:
The Japan
Chemical Journal Forum and Wiley Periodicals, Inc.2008
417:
114:
562:
558:
276:
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549:
1995 Soy Stats. American
Soybean Association Homepage.
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has been solved for this class of enzymes, with the
745:
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168:
149:
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101:
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48:
43:
21:
281:
399:directly attacks the substrate, displacing the
916:
574:
8:
923:
909:
581:
567:
559:
426:species strain B was transferred into the
185:
27:
526:
491:
274:
856:
366:gamma-hexachlorocyclohexane degradation
18:
336:This enzyme belongs to the family of
7:
877:
875:
444:into either the animals or plants.
282:{\displaystyle \rightleftharpoons }
895:. You can help Knowledge (XXG) by
14:
879:
859:
364:. This enzyme participates in
321:. For examples, in the case of
370:1,2-dichloroethane degradation
1:
528:10.1016/S0021-9258(18)96379-9
493:10.1016/S0021-9258(18)97236-4
356:. This enzyme is also called
509:Goldman P, Milne GW (1966).
358:monohaloacetate dehalogenase
354:haloacetate halidohydrolase
968:
947:Enzymes of known structure
874:
452:As of late 2007, only one
362:fluoroacetate dehalogenase
35:Rhodopseudomonas palustris
737:Michaelis–Menten kinetics
434:Butyrivibrio fibrisolvens
184:
26:
629:Diffusion-limited enzyme
420:from the soil bacterium
352:of this enzyme class is
241:haloacetate dehalogenase
38:haloacetate dehalogenase
22:Haloacetate dehalogenase
283:
722:Eadie–Hofstee diagram
655:Allosteric regulation
284:
732:Lineweaver–Burk plot
273:
389:nucleophilic attack
297:of this enzyme are
691:Enzyme superfamily
624:Enzyme promiscuity
474:GOLDMAN P (1965).
448:Structural studies
385:aliphatic compound
309:, whereas its two
289:glycolate + halide
279:
904:
903:
847:
846:
260:chemical reaction
233:
232:
229:
228:
132:metabolic pathway
959:
925:
918:
911:
883:
876:
864:
863:
855:
727:Hanes–Woolf plot
670:Enzyme activator
665:Enzyme inhibitor
639:Enzyme catalysis
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569:
560:
540:
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325:in will produce
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16:Class of enzymes
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962:
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952:Hydrolase stubs
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930:
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755:Oxidoreductases
741:
717:Enzyme kinetics
705:
701:List of enzymes
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643:
614:Catalytic triad
592:
587:
508:
473:
470:
460:accession code
450:
442:B. fibrisolvens
409:
378:
350:systematic name
306:
271:
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265:haloacetate + H
39:
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5:
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680:
679:Classification
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521:(23): 5557–9.
506:
469:
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449:
446:
436:and expressed
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397:water molecule
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344:, exists as a
304:
293:Thus, the two
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891:article is a
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696:Enzyme family
694:
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671:
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660:Cooperativity
658:
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619:Oxyanion hole
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520:
516:
515:J. Biol. Chem
512:
507:
503:
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494:
489:
486:(8): 3434–8.
485:
481:
480:J. Biol. Chem
477:
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447:
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432:
429:
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419:
416:dehalogenase
414:
413:fluoroacetate
406:
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398:
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373:
371:
367:
363:
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332:
328:
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323:fluoroacetate
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170:Gene Ontology
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148:
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136:
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128:
124:
121:
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109:
108:NiceZyme view
106:
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63:
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51:
47:
42:
37:
36:
30:
25:
20:
897:expanding it
886:
871:
833:Translocases
830:
817:
804:
791:
778:
768:Transferases
765:
752:
609:Binding site
518:
514:
483:
479:
451:
441:
433:
421:
410:
407:Significance
393:alpha-carbon
379:
361:
357:
353:
342:human genome
335:
292:
240:
234:
96:BRENDA entry
33:
604:Active site
382:fluorinated
299:haloacetate
84:IntEnz view
67:37289-40-0
44:Identifiers
936:Categories
807:Isomerases
781:Hydrolases
648:Regulation
468:References
338:hydrolases
295:substrates
237:enzymology
153:structures
120:KEGG entry
889:hydrolase
686:EC number
454:structure
431:bacterium
423:Moraxella
376:Reactions
346:homodimer
327:glycolate
315:glycolate
277:⇌
256:catalyzes
73:Databases
942:EC 3.8.1
710:Kinetics
634:Cofactor
597:Activity
502:14321384
438:in vitro
331:fluoride
311:products
250:) is an
224:proteins
212:articles
200:articles
157:RCSB PDB
866:Biology
820:Ligases
590:Enzymes
537:5928195
401:halogen
391:on the
248:3.8.1.3
179:QuickGO
144:profile
127:MetaCyc
62:CAS no.
55:3.8.1.3
852:Portal
794:Lyases
535:
500:
403:atom.
319:halide
252:enzyme
207:PubMed
189:Search
175:AmiGO
163:PDBsum
103:ExPASy
91:BRENDA
79:IntEnz
50:EC no.
887:This
746:Types
428:rumen
254:that
139:PRIAM
893:stub
838:list
831:EC7
825:list
818:EC6
812:list
805:EC5
799:list
792:EC4
786:list
779:EC3
773:list
766:EC2
760:list
753:EC1
533:PMID
498:PMID
462:1Y37
418:gene
368:and
360:and
329:and
317:and
313:are
301:and
258:the
239:, a
219:NCBI
160:PDBe
115:KEGG
523:doi
519:241
488:doi
484:240
458:PDB
411:As
235:In
195:PMC
151:PDB
938::
531:.
517:.
513:.
496:.
482:.
478:.
464:.
372:.
333:.
269:O
245:EC
177:/
924:e
917:t
910:v
899:.
854::
840:)
836:(
827:)
823:(
814:)
810:(
801:)
797:(
788:)
784:(
775:)
771:(
762:)
758:(
582:e
575:t
568:v
539:.
525::
504:.
490::
307:O
305:2
303:H
267:2
243:(
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