895:
441:
556:
292:
726:
838:, the time to pellet in the other rotor, can be calculated. In this manner, one does not need access to the exact rotor cited in a protocol, as long as the K-factor can be calculated.
307:
498:
614:
124:
185:
150:
836:
809:
782:
755:
656:
636:
579:
490:
467:
173:
84:
57:
936:
39:
represents the relative pelleting efficiency of a given centrifuge rotor at maximum rotation speed. It can be used to estimate the time
663:
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883:
960:
922:
64:
658:
is a constant for a certain particle, this relationship can be used to interconvert between different rotors.
436:{\displaystyle k={\frac {2.53\cdot 10^{5}\times \ln(r_{\rm {max}}/r_{\rm {min}})}{({\rm {{RPM}/1000)^{2}}}}}}
955:
551:{\displaystyle k_{\rm {adj}}=k\left({\frac {\mbox{maximum rotor-speed}}{\mbox{actual rotor-speed}}}\right)}
298:
287:{\displaystyle k={\frac {\ln(r_{\rm {max}}/r_{\rm {min}})}{\omega ^{2}}}\times {\frac {10^{13}}{3600}}}
586:
96:
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449:
manufacturers usually specify the minimum, maximum and average radius of a rotor, as well as the
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28:
949:
60:
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472:
For runs with a rotational speed lower than the maximum rotor-speed, the
87:
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As the rotational speed of a centrifuge is usually specified in
842:
are available to perform the calculations for common rotors.
721:{\displaystyle {\frac {T_{1}}{K_{1}}}={\frac {T_{2}}{K_{2}}}}
638:
is the time to pellet a certain particle in hours. Since
561:
The K-factor is related to the sedimentation coefficient
129:
The value of the clearing factor depends on the maximum
910:
301:, the following formula is often used for convenience:
152:
of a centrifuge (in rad/s) and the minimum and maximum
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72:
45:
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930:
8:
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923:
469:factor of a centrifuge-rotor combination.
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811:is the K-factor of the other rotor, and
757:is the time to pellet in one rotor, and
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609:{\displaystyle T={\frac {K}{S}}}
119:{\displaystyle t={\frac {k}{s}}}
784:is the K-factor of that rotor.
884:Cell Biology Laboratory Manual
419:
394:
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1:
874:Beckman Coulter lab resources
909:. You can help Knowledge by
492:factor has to be adjusted:
63:of a fraction with a known
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859:"Basics of Centrifugation"
65:sedimentation coefficient
18:K-factor (centrifugation)
59:(in hours) required for
840:Many online calculators
145:{\displaystyle \omega }
905:–related article is a
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831:{\displaystyle T_{2}}
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804:{\displaystyle K_{2}}
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777:{\displaystyle K_{1}}
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750:{\displaystyle T_{1}}
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961:Fluid dynamics stubs
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534:maximum rotor-speed
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651:{\displaystyle S}
631:{\displaystyle T}
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574:{\displaystyle S}
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485:{\displaystyle k}
462:{\displaystyle k}
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168:{\displaystyle r}
114:
79:{\displaystyle s}
52:{\displaystyle t}
16:(Redirected from
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131:angular velocity
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33:clearing factor
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956:Centrifugation
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868:External links
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29:centrifugation
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61:sedimentation
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911:expanding it
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876:and manuals
950:Categories
880:Appendix F
846:References
447:Centrifuge
343:
337:×
324:⋅
265:×
254:ω
202:
140:ω
88:svedbergs
37:k factor
882:of the
175:of the
730:Where
618:Where
154:radius
901:This
177:rotor
907:stub
416:1000
321:2.53
280:3600
86:(in
31:the
299:RPM
90:):
35:or
27:In
952::
340:ln
328:10
275:13
271:10
199:ln
179::
938:e
931:t
924:v
913:.
824:2
820:T
797:2
793:K
770:1
766:K
743:1
739:T
712:2
708:K
702:2
698:T
692:=
685:1
681:K
675:1
671:T
646:S
626:T
602:S
599:K
594:=
591:T
569:S
545:)
528:(
524:k
521:=
515:j
512:d
509:a
504:k
480:k
457:k
424:2
420:)
412:/
407:M
404:P
401:R
395:(
390:)
384:n
381:i
378:m
373:r
368:/
361:x
358:a
355:m
350:r
346:(
332:5
315:=
312:k
258:2
249:)
243:n
240:i
237:m
232:r
227:/
220:x
217:a
214:m
209:r
205:(
193:=
190:k
163:r
112:s
109:k
104:=
101:t
74:s
47:t
20:)
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