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414:. Derivatives of Pacific Blue have been used to study small molecule-protein interactions using fluorescence resonance energy transfer (FRET). Pacific Blue has also been used to study interactions of the anticancer drug paclitaxel (Taxol) with tubulin in living cells. Reactive forms in use include Pacific Blue succinimidyl
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Pacific Blue is a member of the group of
Pacific dyes, which includes Pacific Orange, Pacific Green, and Pacific Blue. These fluorescent dyes all have an absorption maximum between 400 and 410 nm, but with different emission spectra: this allows simultaneous excitation with one laser, producing
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In flow cytometry
Pacific Blue (as well as other Pacific dyes) absorbs near 405 nm, disallowing simultaneous use with other fluorophores which share similar excitation and/or emission spectra, such as Brilliant Violet 421 (BV421) or V450.
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maximum is at 452 nm. In contrast to the less acidic 7-hydroxy-3-carboxycoumarin (pKa=7.0), the high acidity of the phenol of
Pacific Blue (pKa=3.7) causes its fluorescence to remain very high at neutral pH.
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emission at maxima of 551nm, 500 nm and 455 nm, respectively (note: absorption and emission maxima may vary depending on the manufacturer).
570:"Synthesis of a Fluorescent Analogue of Paclitaxel that Selectively Binds Microtubules and Sensitively Detects Efflux by P-Glycoprotein"
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521:"Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore"
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Lee, Wei-Chuan (1998). "Synthesis of Novel
Fluorinated Coumarins: Excellent UV-Light Excitable Fluorescent Dyes".
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or targeted staining in general, reactive forms of
Pacific Blue are used to label targeting
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InChI=1S/C10H4F2O5/c11-5-2-3-1-4(9(14)15)10(16)17-8(3)6(12)7(5)13/h1-2,13H,(H,14,15)
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Except where otherwise noted, data are given for materials in their
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475:"Absorption and Emission Spectra — Pacific Blue™"
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441:Bioorganic & Medicinal Chemistry Letters
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574:Angewandte Chemie International Edition
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244:Key: VYNDHICBIRRPFP-UHFFFAOYSA-N
234:Key: DLBFLQKQABVKGT-UHFFFAOYSA-L
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385:maximum lies at 401 nm, while its
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332:(at 25 °C , 100 kPa).
265:OC(=O)c1cc2cc(F)c(O)c(F)c2oc1=O
45:-1-benzopyran-3-carboxylic acid
506:"Violet Proliferation Dye 450"
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41:6,8-Difluoro-7-hydroxy-2-oxo-2
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453:10.1016/s0960-894x(98)00578-2
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494:. ThermoFisher Scientific.
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537:10.1021/acsomega.6b00356
586:10.1002/anie.201703298
568:Lee, Molly M. (2017).
519:Lee, Molly M. (2016).
418:and Pacific Blue C5-
408:immunohistochemistry
37:Preferred IUPAC name
322: g·mol
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492:"Pacific Blue dye"
359:Infobox references
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508:. BD Biosciences.
477:. BD Biosciences.
367:Chemical compound
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190:CompTox Dashboard
93:Interactive image
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379:cell biology
371:Pacific Blue
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133:ChEMBL104101
52:Identifiers
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375:fluorophore
274:Properties
113:CHEBI:63236
69:215868-31-8
618:Categories
426:References
412:antibodies
383:excitation
315:Molar mass
144:ChemSpider
80:3D model (
59:CAS Number
634:Coumarins
525:ACS Omega
420:Maleimide
604:28485901
555:28058293
387:emission
377:used in
178:18942321
153:13906206
595:5679005
546:5204206
461:9873685
352:what is
350: (
320:242.134
165:PubChem
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381:. Its
347:verify
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258:SMILES
124:ChEMBL
31:Names
416:ester
402:Usage
226:InChI
104:ChEBI
82:JSmol
600:PMID
551:PMID
457:PMID
590:PMC
582:doi
541:PMC
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449:doi
195:EPA
168:CID
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305:O
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43:H
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