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of the solution contains enough virus particles to produce 1000 infectious plaques in a layer of cells. However, the number of virus particles might be greater than the number of PFU if some virus particles fail to form plaques because they are defective or otherwise failed to infect cells. The
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measurement rather than a measurement of the absolute quantity of particles: viral particles that are defective or which fail to infect their target cell will not produce a plaque and thus will not be counted.
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Measure of the number of particles capable of forming plaques per unit volume
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concept of plaque-forming units of virus is equivalent to the concept of
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with a concentration of 1,000 PFU/μL indicates that 1
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