124:: some mutational pathways show steady, additive changes, whereas others show drastic jumps between contrasting product specificities with single mutational steps. Further, a metric was devised to describe the chemical distance of mutational steps to derive a chemical-based
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relating sequence variation to chemical output. These examples establish SCOPE as a standardized method for the construction of synthetic gene libraries from close or distantly related parental sequences to identify functional novelty among the encoded proteins.
43:, and evolution, although the technique is generally applicable for the creation of engineered proteins with commercially desirable properties. Combinatorial travel through sequence
35:) of defined composition designed from structural and probabilistic constraints of the encoded proteins. The development of this technique was driven by fundamental questions about
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lineage, which was biochemically characterized to recapitulate the evolutionary divergence of two modern day enzymes. The rapid evolvability of chemical diversity in
59:-independent recombination technique to enable the creation of multiple crossover libraries from distantly related genes. In this application, an “exon
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O'Maille PE, Tsai MD, Greenhagen BT, Chappell J, Noel JP (2004). "Gene library synthesis by structure-based combinatorial protein engineering".
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O'Maille PE, Malone A, Dellas N, Andes Hess B, Smentek L, Sheehan I, Greenhagen BT, Chappell J, Manning G, Noel JP (Oct 2008).
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O'Maille PE, Bakhtina M, Tsai MD (Aug 2002). "Structure-based combinatorial protein engineering (SCOPE)".
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were demonstrated through processes akin to both
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strategy to selectively cross hybrid genes, a process of
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67:) with variability in the junctions linking them (
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55:At its inception, SCOPE was developed as a
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250:10.1016/S0076-6879(04)88008-X
225:10.1016/S0022-2836(02)00675-7
213:Journal of Molecular Biology
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87:in temperature-sensitive
340:Combinatorial chemistry
281:Nature Chemical Biology
85:Genetic complementation
27:technique for creating
164:Nucleic acid analogues
47:is the goal of SCOPE.
149:Expanded genetic code
345:Evolutionary biology
293:10.1038/nchembio.113
350:Protein engineering
242:Protein Engineering
174:Protein engineering
75:was adapted into a
194:Structural biology
139:Directed evolution
65:continental plates
355:Synthetic biology
199:Synthetic biology
37:protein structure
25:synthetic biology
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69:fault lines
51:Description
334:Categories
184:Proteomics
144:Enzymology
118:gradualism
100:phenotypes
126:phylogeny
122:saltation
114:synthases
81:iterative
45:spacetime
311:18776889
268:15289063
233:12206782
189:Proteome
133:See also
57:homology
41:function
33:lineages
302:2664519
111:terpene
89:E. coli
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159:Genome
107:enzyme
93:hybrid
21:SCOPE
307:PMID
264:PMID
254:ISBN
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246:doi
221:doi
217:321
77:PCR
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Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.