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that produces a voltage depending on the instantaneous ratio of local concentrations. While the concentrations are fluctuating, an alternating random voltage difference will appear between the electrodes. According to the experimental studies, whenever there is an ongoing phage infection, the power
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as indicators to trigger an ionic response by the bacteria during phage infection. Microscopic metal electrodes detect the random fluctuations of the electrochemical potential due to the stochastic fluctuations of the ionic concentration gradient caused by the phage infection of bacteria. The
81:, and durability originating from the robustness of phages. An idealistic SEPTIC device may be as small as a pen and maybe able to identify a library of different bacteria within a few minutes measurement window.
31:, and durability originating from the robustness of phages. An idealistic SEPTIC device may be as small as a pen and maybe able to identify a library of different bacteria within a few minutes measurement window.
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spectrum. In order to have a high sensitivity, a DC electrical field attracts the infected bacteria (which are charged due to ion imbalance) to the electrode with the relevant polarization.
190:"L.B. Kish, M. Cheng, J.U. Kim, S. Seo, M.D. King, R. Young, A. Der, G. Schmera, "Estimation of the detection limits of the phage-invasion based identification of bacteria",
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in fluid medium. The advantages of SEPTIC are the specificity and speed (needs only a few minutes) offered by the characteristics of phage infection, the sensitivity due to
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166:"M. Dobozi-King, S. Seo, J.U. Kim, R. Young, M. Cheng, L.B. Kish, "Rapid detection and identification of bacteria: SEnsing of Phage-Triggered Ion Cascade (SEPTIC)",
217:"J.R. Biard, L.B. Kish, "Enhancing the sensitivity of the SEPTIC bacterium sensing method by concentrating the phage-infected bacteria via DC electrical current",
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The advantages of SEPTIC are the specificity and speed (needs only a few minutes) offered by the characteristics of phage infection, the sensitivity due to
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109:) utilizes the AC impedance fluctuations at related arrangements, and it promises higher sensitivity and less dependence on the electrodes.
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Gabor
Schmera; Kish (2010). "G. Schmera, L.B. Kish, "Bacteria Identification by Phage Induced Impedance Fluctuation Analysis (BIPIF)",
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and Maria Dobozi-King in 2004, and developed and demonstrated at the
Electrical and Computer Engineering department of
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Homepage of the SEPTIC method at the
Department of Electrical and Computer Engineering, Texas A&M University
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electrode pair in the electrolyte with different local ion concentrations at the vicinity of electrodes form an
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101:(NIH), and Bob Biard. A new, related scheme, BIPIF, that has recently been conceived and analyzed by
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Ry Young's phage lab at the
Department of Biochemistry and Biophysics, Texas A&M University
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23:) is a prompt bacterium identification method based on
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168:Journal of Biological Physics and Chemistry
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89:The SEPTIC concept was first conceived by
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65:while, without phage infection, it is a
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17:Sensing of phage-triggered ion cascades
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269:"Featuring SEPTIC in Nanotechwire"
127:"The SEPTIC patent description in
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49:density spectrum of the measured
311:Biological techniques and tools
148:"Feature article on SEPTIC in
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219:Fluctuation and Noise Letters
192:Fluctuation and Noise Letters
79:fluctuation-enhanced sensing
29:fluctuation-enhanced sensing
25:fluctuation-enhanced sensing
105:and Gabor Schmera (SPAWAR,
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296:SEPTIC in Acronym Finder
95:Texas A&M University
245:US Navy patent pending
316:Laboratory equipment
247:; to be published".
46:electrochemical cell
107:United States Navy
99:Sergey M. Bezrukov
224:(2005) L153-L158"
197:(2005) L105-L108"
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154:. 14 April 2005.
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52:electronic noise
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103:Laszlo B. Kish
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63:noise spectrum
41:bacteriophages
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173:(2005) 3–7"
129:PATENTSCOPE
305:Categories
113:References
73:Advantages
254:1004.0147
152:magazine"
67:1/f noise
85:History
21:SEPTIC
249:arXiv
227:(PDF)
200:(PDF)
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