185:
each patterned with 150,000 ZMW holes that were read in two sets of 75,000. In April 2013, the company released a new version of the sequencer called the "PacBio RS II" that uses all 150,000 ZMW holes concurrently, doubling the throughput per experiment. The highest throughput mode in
November 2013 used P5 binding, C3 chemistry, BluePippin size selection, and a PacBio RS II officially yielded 350 million bases per SMRT Cell though a human
62:. Each of the four DNA bases is attached to one of four different fluorescent dyes. When a nucleotide is incorporated by the DNA polymerase, the fluorescent tag is cleaved off and diffuses out of the observation area of the ZMW where its fluorescence is no longer observable. A detector detects the fluorescent signal of the nucleotide incorporation, and the base call is made according to the corresponding fluorescence of the dye.
269:
294:
158:
489:. Scientists demonstrated the use of single-molecule real-time sequencing for detecting methylation and other base modifications. In 2012 a team of scientists used SMRT sequencing to generate the full methylomes of six bacteria. In November 2012, scientists published a report on genome-wide methylation of an outbreak strain of E. coli.
363:
and 67.4 GB yield per cell with templates in higher weight molecules. System performance is now reported in either high-molecular-weight continuous long reads or in pre-corrected HiFi (also known as
Circular Consensus Sequence (CCS)) reads. For high-molecular-weight reads roughly half of all reads are longer than 50 kb in length.
478:
automated finishing of bacterial genomes, including one paper that updated the Celera
Assembler with a pipeline for genome finishing using long SMRT sequencing reads. In 2013, scientists estimated that long-read sequencing could be used to fully assemble and finish the majority of bacterial and archaeal genomes.
70:
The DNA sequencing is done on a chip that contains many ZMWs. Inside each ZMW, a single active DNA polymerase with a single molecule of single stranded DNA template is immobilized to the bottom through which light can penetrate and create a visualization chamber that allows monitoring of the activity
362:
In April 2019 the company released a new SMRT Cell with eight million ZMWs, increasing the expected throughput per SMRT Cell by a factor of eight. Early access customers in March 2019 reported throughput over 58 customer run cells of 250 GB of raw yield per cell with templates about 15 kb in length,
481:
The same DNA molecule can be resequenced independently by creating the circular DNA template and utilizing a strand displacing enzyme that separates the newly synthesized DNA strand from the template. In August 2012, scientists from the Broad
Institute published an evaluation of SMRT sequencing for
180:
On
October 15, 2014, PacBio announced the release of new chemistry P6-C4 for the RS II system, which represents the company's 6th generation of polymerase and 4th generation chemistry--further extending the average read length to 10,000 - 15,000 bases, with the longest reads exceeding 40,000 bases.
184:
Throughput per experiment for the technology is both influenced by the read length of DNA molecules sequenced as well as total multiplex of a SMRT Cell. The prototype of the SMRT Cell contained about 3000 ZMW holes that allowed parallelized DNA sequencing. At commercialization, the SMRT Cells were
176:
On
October 3, 2013, PacBio released new reagent combination for PacBio RS II, the P5 DNA polymerase with C3 chemistry (P5-C3). Together, they extend sequencing read lengths to an average of approximately 8,500 bases, with the longest reads exceeding 30,000 bases. Throughput per SMRT cell is around
172:
On August 21, 2013, PacBio released a new DNA polymerase
Binding Kit P4. This P4 enzyme has average read lengths of more than 4,300 bases when paired with the C2 sequencing chemistry and more than 5,000 bases when paired with the XL chemistry. The enzyme’s accuracy is similar to C2, reaching QV50
137:
released the Sequel 6.0 chemistry, synchronizing the chemistry version with the software version. Performance is contrasted for large-insert libraries with high molecular weight DNA versus shorter-insert libraries below ~15,000 bases in length. For larger templates average read lengths are up to
495:
SMRT sequencing has several applications in reproductive medical genetics research when investigating families with suspected parental gonadal mosaicism. Long reads enable haplotype phasing in patients to investigate parent-of-origin of mutations. Deep sequencing enables determination of allele
173:
between 30X and 40X coverage. The resulting P4 attributes provided higher-quality assemblies using fewer SMRT Cells and with improved variant calling. When coupled with input DNA size selection (using an electrophoresis instrument such as BluePippin) yields average read length over 7 kilobases.
477:
genome sequencing and easier genome assemblies. Scientists are also using single-molecule real-time sequencing in hybrid assemblies for de novo genomes to combine short-read sequence data with long-read sequence data. In 2012, several peer-reviewed publications were released demonstrating the
301:
On 19 September 2018, the company announced the Sequel 6.0 chemistry with average read lengths increased to 100,000 bases for shorter-insert libraries and 30,000 for longer-insert libraries. SMRT Cell yield increased up to 50 billion bases for shorter-insert libraries.
189:
data set released with the chemistry averaging 500 million bases per SMRT Cell. Throughput varies based on the type of sample being sequenced. With the introduction of P6-C4 chemistry typical throughput per SMRT Cell increased to 500 million bases to 1 billion bases.
285:
On March 8, 2018, the 2.1 chemistry was released. It increased average read length to 20,000 bases and half of all reads above 30,000 bases in length. Yield per SMRT Cell increased to 10 or 20 billion bases, for either large-insert libraries or shorter-insert (e.g.
125:
Sequencing performance can be measured in read length, accuracy, and total throughput per experiment. PacBio sequencing systems using ZMWs have the advantage of long read lengths, although error rates are on the order of 5-15% and sample throughput is lower than
165:
At commercialization, read length had a normal distribution with a mean of about 1100 bases. A new chemistry kit released in early 2012 increased the sequencer's read length; an early customer of the chemistry cited mean read lengths of 2500 to 2900 bases.
138:
30,000 bases. For shorter-insert libraries, average read length are up to 100,000 bases while reading the same molecule in a circle several times. The latter shorter-insert libraries then yield up to 50 billion bases from a single SMRT Cell.
91:. The fluorescent dye molecule is attached to the phosphate chain of the nucleotide. When the nucleotide is incorporated by the DNA polymerase, the fluorescent dye is cleaved off with the phosphate chain as a part of a natural
71:
of the DNA polymerase at a single molecule level. The signal from a phospho-linked nucleotide incorporated by the DNA polymerase is detected as the DNA synthesis proceeds which results in the DNA sequencing in real time.
181:
The throughput with the new chemistry was estimated between 500 million to 1 billion bases per SMRT Cell, depending on the sample being sequenced. This was the final version of chemistry released for the RS instrument.
53:
enzyme is affixed at the bottom of a ZMW with a single molecule of DNA as a template. The ZMW is a structure that creates an illuminated observation volume that is small enough to observe only a single
114:
The ZMW holes are ~70 nm in diameter and ~100 nm in depth. Due to the behavior of light when it travels through a small aperture, the optical field decays exponentially inside the chamber.
117:
The observation volume within an illuminated ZMW is ~20 zeptoliters (20 X 10 liters). Within this volume, the activity of DNA polymerase incorporating a single nucleotide can be readily detected.
87:
For each of the nucleotide bases, there is a corresponding fluorescent dye molecule that enables the detector to identify the base being incorporated by the DNA polymerase as it performs the
402:
The HiFi performance includes corrected bases with quality above Phred score Q20, using repeated amplicon passes for correction. These take amplicons up to 20kb in length.
99:
is created to elongate the DNA chain. The cleaved fluorescent dye molecule then diffuses out of the detection volume so that the fluorescent signal is no longer detected.
1415:
1177:
492:
Long reads make it possible to sequence full gene isoforms, including the 5' and 3' ends. This type of sequencing is useful to capture isoforms and splice variants.
2315:
684:
2422:"A Novel Approach Using Long-Read Sequencing and ddPCR to Investigate Gonadal Mosaicism and Estimate Recurrence Risk in Two Families With Developmental Disorders"
1310:
469:
genome sequencing, read lengths from the single-molecule real-time sequencing are comparable to or greater than that from the Sanger sequencing method based on
1440:
1342:
1041:
999:
978:
276:
In
September 2015, the company announced the launch of a new sequencing instrument, the Sequel System, that increased capacity to 1 million ZMW holes.
1020:
1242:
828:"Zero-mode waveguides can be made better: fluorescence enhancement with rectangular aluminum nanoapertures from the visible to the deep ultraviolet"
1456:
1213:
1178:"Pacific Biosciences Releases New DNA Sequencing Chemistry to Enhance Read Length and Accuracy for the Study of Human and Other Complex Genomes"
630:
1361:
957:
2476:
680:"Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures"
1379:
1082:
657:
512:
Levene MJ, Korlach J, Turner SW, et al. (2003). "Zero-Mode
Waveguides for Single-Molecule Analysis at High Concentrations".
279:
With the Sequel instrument initial read lengths were comparable to the RS, then later chemistry releases increased read length.
2496:
1535:
1485:
739:
Foquet M, Samiee KT, Kong X, et al. (2008). "Improved fabrication of zero-mode waveguides for single-molecule detection".
2211:"Genome-wide Mapping of Methylated Adenine Residues in Pathogenic Escherichia Coli Using Single-Molecule Real-Time Sequencing"
1419:
111:
confinement structure that consists of a circular hole in an aluminum cladding film deposited on a clear silica substrate.
282:
On
January 23, 2017, the V2 chemistry was released. It increased average read lengths to between 10,000 and 18,000 bases.
826:
Baibakov, Mikhail; Barulin, Aleksandr; Roy, Prithu; Claude, Jean-Benoît; Patra, Satyajit; Wenger, Jérôme (1999-02-22).
1318:
1580:
741:
149:(PacBio) commercialized SMRT sequencing in 2011, after releasing a beta version of its RS instrument in late 2010.
1603:
1397:
2372:"Single Molecule Real-Time (SMRT) Sequencing Comes of Age: Applications and Utilities for Medical Diagnostics"
1100:
2491:
1063:
1273:
2376:
2165:
2012:
496:
frequencies in sperm cells, of relevance for estimation of recurrence risk for future affected offspring.
2008:"Characterization of DNA Methyltransferase Specificities Using Single-Molecule, Real-Time DNA Sequencing"
1250:
568:
Eid J, Fehr A, Gray J, et al. (2009). "Real-Time DNA Sequencing from Single Polymerase Molecules".
1042:"PacBio Users Report Progress in Long Reads for Plant Genome Assembly, Tricky Regions of Human Genome"
2324:
2265:
2215:
1859:
1798:
1730:
1680:
1221:
1133:
1000:"After a Year of Testing, Two Early PacBio Customers Expect More Routine Use of RS Sequencer in 2012"
979:"PacBio Reveals Beta System Specs for RS; Says Commercial Release is on Track for First Half of 2011"
888:
749:
693:
579:
523:
877:"The Madness of Microbiome: Attempting To Find Consensus "Best Practice" for 16S Microbiome Studies"
79:
To prepare the library, DNA fragments are put into a circular form using hairpin adapter ligations.
17:
783:
Zhu, Paul; Craighead, Harold G. (2012-06-09). "Zero-Mode Waveguides for Single-Molecule Analysis".
146:
134:
96:
46:
169:
The XL chemistry kit released in late 2012 increased average read length to more than 4300 bases.
1788:
1021:"PacBio's XL Chemistry Increases Read Lengths and Throughput; CSHL Tests the Tech on Rice Genome"
765:
603:
547:
470:
1905:"Pacific Biosciences Sequencing Technology for Genotyping and Variation Discovery in Human Data"
1101:"New Chemistry for PacBio RS II Provides Average 8.5 kb Read Lengths for Complex Genome Studies"
1292:
1195:
462:
Single-molecule real-time sequencing may be applicable for a broad range of genomics research.
2501:
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2402:
2352:
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2241:
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2141:
2089:
2038:
1988:
1937:
1885:
1846:"Validation of ITD mutations in FLT3 as a therapeutic target in human acute myeloid leukaemia"
1826:
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527:
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2007:
1983:
1961:
1956:
1932:
1904:
1880:
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1821:
1780:
1775:
1751:
1725:
1701:
1675:
1651:
1625:
1556:
1530:
1506:
1476:
1362:"New Chemistry and Software for Sequel System Improve Read Length, Lower Project Costs"
1154:
1119:
911:
876:
852:
827:
716:
679:
127:
59:
50:
42:
2485:
92:
88:
769:
607:
1909:
1776:"Reducing assembly complexity of microbial genomes with single-molecule sequencing"
551:
1957:"Direct detection of DNA methylation during single-molecule, real-time sequencing"
1726:"Hybrid error correction and de novo assembly of single-molecule sequencing reads"
1457:"PacBio Shares Early-Access Customer Experiences, New Applications for Sequel II"
1343:"PacBio Launches Higher-Throughput, Lower-Cost Single-Molecule Sequencing System"
1064:"New DNA Polymerase P4 Delivers Higher-Quality Assemblies Using Fewer SMRT Cells"
875:
Pollock, Jolinda; Glendinning, Laura; Wisedchanwet, Trong; Watson, Mick (2018).
1380:"New Software, Polymerase for Sequel System Boost Throughput and Affordability"
1120:"Resolving the complexity of the human genome using single-molecule sequencing"
2058:"Sensitive and Specific Single-Molecule Sequencing of 5-hydroxymethylcytosine"
1811:
486:
177:
500 million bases demonstrated by sequencing results from the CHM1 cell line.
55:
1922:
804:
640:
2337:
1196:"New Chemistry Boosts Average Read Length to 10 kb – 15 kb for PacBio RS II"
706:
591:
535:
268:
108:
2455:
2406:
2356:
2295:
2245:
2195:
2145:
2126:
2093:
2042:
1992:
1941:
1889:
1830:
1760:
1710:
1660:
1565:
1515:
1214:"SMRT Cells, sequencing reagent kits, and accessories for the PacBio RS II"
1163:
920:
861:
812:
725:
599:
543:
2024:
1642:
1547:
1497:
2388:
2177:
901:
287:
1871:
1145:
293:
2075:
1974:
843:
658:"Pacific Biosciences Develops Transformative DNA Sequencing Technology"
157:
2311:"Characterization of the human ESC transcriptome by hybrid sequencing"
958:"PacBio Ships First Two Commercial Systems; Order Backlog Grows to 44"
761:
2277:
2227:
1742:
1692:
2437:
1676:"A hybrid approach for the automated finishing of bacterial genomes"
1481:
Strain Causing an Outbreak of Hemolytic–Uremic Syndrome in Germany"
1793:
292:
267:
156:
2261:"A Single-Molecule Long-Read Survey of the Human Transcriptome"
1118:
Chaisson MJ, Huddleston J, Dennis MY, et al. (2014).
485:
The dynamics of polymerase can indicate whether a base is
2420:
Wilbe M, Gudmundsson S, Johansson J, et al. (2017).
45:
method. Single-molecule real-time sequencing utilizes a
1626:"Finished bacterial genomes from shotgun sequence data"
2109:"Direct Detection and Sequencing of Damaged DNA Bases"
1083:"Longing for the longest reads: PacBio and BluePippin"
2107:
Clark TA, Spittle KE, Turner SW, et al. (2011).
1955:
Flusberg BA, Webster DR, Lee JH, et al. (2010).
1624:
Ribeiro FJ, Przybylski D, Yin S, et al. (2012).
2370:
Ardui S, Ameur A, Vermeesch JR, et al. (2018).
2309:
Au KF, Sebastiano V, Afshar PT, et al. (2013).
2259:
Sharon D, Tilgner H, Grubert F, et al. (2013).
2159:
Murray IA, Clark TA, Morgan RD, et al. (2012).
2006:
Clark TA, Murray IA, Morgan RD, et al. (2012).
1674:
Bashir A, Klammer A, Robins WP, et al. (2012).
1529:
Chin CS, Sorenson J, Harris JB, et al. (2011).
678:
Korlach J, Marks PJ, Cicero RL, et al. (2008).
2209:Fang G, Munera D, Friedman DI, et al. (2012).
1724:Koren S, Schatz MC, Walenz BP, et al. (2012).
1531:"The Origin of the Haitian Cholera Outbreak Strain"
1475:Rasko DA, Webster DR, Sahl JW, et al. (2011).
1903:Carneiro MO, Russ C, Ross MG, et al. (2012).
1774:Koren S, Harhay GP, Smith TP, et al. (2013).
1455:
1341:
1272:
1040:
1019:
998:
977:
956:
932:
930:
473:chain termination. The longer read length allows
1844:Smith CC, Wang Q, Chin CS, et al. (2012).
1579:Gao H, Green SJ, Jafari N, et al. (2012).
2056:Song CX, Clark TA, Lu XY, et al. (2011).
563:
561:
8:
1585:Genetic Engineering & Biotechnology News
367:Sequel II High-Molecular-Weight Performance
1311:"PacBio Announces Sequel Sequencing System"
665:Pacific Biosciences Technology Backgrounder
406:Sequel II HiFi Corrected Read Performance
404:
365:
304:
192:
2445:
2396:
2346:
2336:
2285:
2235:
2185:
2135:
2125:
2083:
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1982:
1931:
1921:
1879:
1820:
1810:
1792:
1750:
1700:
1650:
1555:
1505:
1274:"New Products: PacBio's RS II; Cufflinks"
1153:
910:
900:
851:
715:
705:
1243:"PacBio Launches PacBio RS II Sequencer"
1581:"Tech Tips: Next-Generation Sequencing"
504:
441:Corrected reads per SMRT Cell (>Q20)
1439:: CS1 maint: archived copy as title (
1432:
881:Applied and Environmental Microbiology
1058:
1056:
797:10.1146/annurev-biophys-050511-102338
161:SMRT Cell for a RS or RS II Sequencer
7:
652:
650:
18:Single Molecule Real Time Sequencing
107:The zero-mode waveguide (ZMW) is a
1398:"PacBio Launches Sequel II System"
41:is a parallelized single molecule
25:
2161:"The Methylomes of Six Bacteria"
272:SMRT Cell for a Sequel Sequencer
2472:Report from the BioIT World.com
791:(1). Annual Reviews: 269–293.
625:(in Dutch). Oxford: Academic.
297:Pipette tip in an 8M SMRT Cell
1:
1317:. 30 Sep 2015. Archived from
1249:. 11 Apr 2013. Archived from
1184:(Press Release). 15 Oct 2014.
1081:lexnederbragt (19 Jun 2013).
58:of DNA being incorporated by
621:Friedmann, Theodore (2012).
785:Annual Review of Biophysics
2518:
2477:Report from New York Times
1604:"SMRT-assembly approaches"
290:) libraries respectively.
1812:10.1186/gb-2013-14-9-r101
325:Average read length bases
219:Average read length bases
31:Single-molecule real-time
27:Method for sequencing DNA
1923:10.1186/1471-2164-13-375
1087:In between lines of code
386:Throughput per SMRT Cell
341:Throughput per SMRT Cell
241:Throughput per SMRT Cell
83:Phospholinked nucleotide
2338:10.1073/pnas.1320101110
1613:(PacBio Users Meeting).
1602:Schatz M (7 Sep 2011).
1018:Heger M (13 Nov 2012).
997:Karow J (10 Jan 2012).
707:10.1073/pnas.0710982105
592:10.1126/science.1162986
536:10.1126/science.1079700
425:Raw reads per SMRT Cell
95:process during which a
2497:DNA sequencing methods
2127:10.1186/2041-9414-2-10
1454:Heger M (7 Mar 2019).
1340:Heger M (1 Oct 2015).
1293:"Duke Sequencing Post"
1220:. 2020. Archived from
1039:Heger M (5 Mar 2013).
976:Karow J (7 Dec 2010).
955:Karow J (3 May 2011).
748:(3): 034301–034301–9.
298:
273:
162:
130:sequencing platforms.
121:Sequencing Performance
1643:10.1101/gr.141515.112
1548:10.1056/NEJMoa1012928
1498:10.1056/NEJMoa1106920
296:
271:
160:
902:10.1128/AEM.02627-17
623:Advances in genetics
75:Template preparation
2329:2013PNAS..110E4821A
2025:10.1093/nar/gkr1146
1872:10.1038/nature11016
1864:2012Natur.485..260S
1803:2013arXiv1304.3752K
1253:on 19 December 2019
1218:Pacific Biosciences
1182:Pacific Biosciences
1146:10.1038/nature13907
1138:2015Natur.517..608C
893:2018ApEnM..84E2627P
754:2008JAP...103c4301F
698:2008PNAS..105.1176K
584:2009Sci...323..133E
528:2003Sci...299..682L
407:
368:
307:
306:Sequel Performance
195:
147:Pacific Biosciences
135:Pacific Biosciences
103:Zero-Mode Waveguide
97:phosphodiester bond
47:zero-mode waveguide
2426:Prenatal Diagnosis
2389:10.1093/nar/gky066
2377:Nucleic Acids Res.
2178:10.1093/nar/gks891
2166:Nucleic Acids Res.
2076:10.1038/nmeth.1779
2013:Nucleic Acids Res.
1975:10.1038/nmeth.1459
1611:schatzlab.cshl.edu
844:10.1039/D0NA00366B
832:Nanoscale Advances
405:
366:
305:
299:
274:
193:
163:
762:10.1063/1.2831366
632:978-0-12-394395-8
471:dideoxynucleotide
455:
454:
400:
399:
355:
354:
335:30,000 - 100,000
261:
260:
16:(Redirected from
2509:
2460:
2459:
2449:
2417:
2411:
2410:
2400:
2367:
2361:
2360:
2350:
2340:
2323:(50): E4821–30.
2306:
2300:
2299:
2289:
2278:10.1038/nbt.2705
2266:Nat. Biotechnol.
2256:
2250:
2249:
2239:
2228:10.1038/nbt.2432
2216:Nat. Biotechnol.
2206:
2200:
2199:
2189:
2172:(22): 11450–62.
2156:
2150:
2149:
2139:
2129:
2104:
2098:
2097:
2087:
2053:
2047:
2046:
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2003:
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1835:
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1814:
1796:
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1754:
1743:10.1038/nbt.2280
1731:Nat. Biotechnol.
1721:
1715:
1714:
1704:
1693:10.1038/nbt.2288
1681:Nat. Biotechnol.
1671:
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1615:
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1608:
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1536:N. Engl. J. Med.
1526:
1520:
1519:
1509:
1486:N. Engl. J. Med.
1477:"Origins of the
1472:
1466:
1465:
1459:
1451:
1445:
1444:
1438:
1430:
1428:
1427:
1418:. Archived from
1412:
1406:
1405:
1394:
1388:
1387:
1376:
1370:
1369:
1358:
1352:
1351:
1345:
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1326:
1307:
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1300:
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1239:
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1229:
1210:
1204:
1203:
1192:
1186:
1185:
1174:
1168:
1167:
1157:
1132:(7536): 608–11.
1115:
1109:
1108:
1097:
1091:
1090:
1078:
1072:
1071:
1060:
1051:
1050:
1044:
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1008:
1002:
994:
988:
987:
981:
973:
967:
966:
960:
952:
946:
945:
934:
925:
924:
914:
904:
887:(7): e02627-17.
872:
866:
865:
855:
838:(9): 4153–4160.
823:
817:
816:
780:
774:
773:
736:
730:
729:
719:
709:
675:
669:
668:
662:
654:
645:
644:
618:
612:
611:
565:
556:
555:
509:
408:
369:
332:20,000 - 30,000
329:10,000 - 18,000
308:
235:10,000 - 15,000
196:
133:On 19 Sep 2018,
49:(ZMW). A single
21:
2517:
2516:
2512:
2511:
2510:
2508:
2507:
2506:
2482:
2481:
2468:
2463:
2438:10.1002/pd.5156
2432:(11): 1146–54.
2419:
2418:
2414:
2369:
2368:
2364:
2308:
2307:
2303:
2272:(11): 1009–14.
2258:
2257:
2253:
2208:
2207:
2203:
2158:
2157:
2153:
2106:
2105:
2101:
2055:
2054:
2050:
2005:
2004:
2000:
1954:
1953:
1949:
1902:
1901:
1897:
1858:(7397): 260–3.
1843:
1842:
1838:
1773:
1772:
1768:
1723:
1722:
1718:
1673:
1672:
1668:
1623:
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1618:
1606:
1601:
1600:
1596:
1578:
1577:
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1528:
1527:
1523:
1474:
1473:
1469:
1453:
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1448:
1431:
1425:
1423:
1416:"Archived copy"
1414:
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1396:
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1391:
1378:
1377:
1373:
1360:
1359:
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1324:
1322:
1321:on 29 July 2020
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975:
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874:
873:
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777:
738:
737:
733:
677:
676:
672:
660:
656:
655:
648:
633:
620:
619:
615:
578:(5910): 133–8.
567:
566:
559:
522:(5607): 682–6.
511:
510:
506:
502:
460:
360:
266:
194:RS Performance
155:
144:
123:
105:
85:
77:
68:
28:
23:
22:
15:
12:
11:
5:
2515:
2513:
2505:
2504:
2499:
2494:
2492:Bioinformatics
2484:
2483:
2480:
2479:
2474:
2467:
2466:External links
2464:
2462:
2461:
2412:
2383:(5): 2159–68.
2362:
2301:
2251:
2222:(12): 1232–9.
2201:
2151:
2114:Genome Integr.
2099:
2048:
1998:
1947:
1895:
1836:
1766:
1737:(7): 693–700.
1716:
1666:
1637:(11): 2270–7.
1616:
1594:
1571:
1521:
1467:
1446:
1407:
1404:. 26 Apr 2019.
1389:
1371:
1353:
1332:
1302:
1299:. 30 Aug 2013.
1284:
1281:. 16 Apr 2013.
1264:
1234:
1205:
1202:. 15 Oct 2014.
1187:
1169:
1110:
1092:
1073:
1070:. 21 Aug 2013.
1052:
1031:
1010:
989:
968:
947:
944:. 19 Sep 2018.
926:
867:
818:
775:
742:J. Appl. Phys.
731:
692:(4): 1176–81.
670:
646:
631:
613:
557:
503:
501:
498:
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199:
154:
151:
143:
140:
122:
119:
104:
101:
84:
81:
76:
73:
67:
64:
60:DNA polymerase
51:DNA polymerase
43:DNA sequencing
26:
24:
14:
13:
10:
9:
6:
4:
3:
2:
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2255:
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2197:
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2188:
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2179:
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2171:
2168:
2167:
2162:
2155:
2152:
2147:
2143:
2138:
2133:
2128:
2123:
2119:
2116:
2115:
2110:
2103:
2100:
2095:
2091:
2086:
2081:
2077:
2073:
2069:
2065:
2064:
2059:
2052:
2049:
2044:
2040:
2035:
2030:
2026:
2022:
2018:
2015:
2014:
2009:
2002:
1999:
1994:
1990:
1985:
1980:
1976:
1972:
1968:
1964:
1963:
1958:
1951:
1948:
1943:
1939:
1934:
1929:
1924:
1919:
1915:
1912:
1911:
1906:
1899:
1896:
1891:
1887:
1882:
1877:
1873:
1869:
1865:
1861:
1857:
1853:
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1847:
1840:
1837:
1832:
1828:
1823:
1818:
1813:
1808:
1804:
1800:
1795:
1790:
1786:
1783:
1782:
1777:
1770:
1767:
1762:
1758:
1753:
1748:
1744:
1740:
1736:
1733:
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1727:
1720:
1717:
1712:
1708:
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1682:
1677:
1670:
1667:
1662:
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1572:
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1538:
1537:
1532:
1525:
1522:
1517:
1513:
1508:
1503:
1499:
1495:
1492:(8): 709–17.
1491:
1488:
1487:
1482:
1480:
1471:
1468:
1463:
1458:
1450:
1447:
1442:
1436:
1422:on 2018-09-24
1421:
1417:
1411:
1408:
1403:
1399:
1393:
1390:
1386:. 7 Mar 2018.
1385:
1381:
1375:
1372:
1368:. 9 Jan 2017.
1367:
1363:
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1349:
1344:
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1303:
1298:
1294:
1288:
1285:
1280:
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1268:
1265:
1252:
1248:
1247:Next Gen Seek
1244:
1238:
1235:
1224:on 2013-04-21
1223:
1219:
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1209:
1206:
1201:
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1156:
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1143:
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1131:
1127:
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1121:
1114:
1111:
1107:. 3 Oct 2013.
1106:
1102:
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1074:
1069:
1065:
1059:
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1006:
1001:
993:
990:
985:
980:
972:
969:
964:
959:
951:
948:
943:
939:
938:"PacBio Post"
933:
931:
927:
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918:
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890:
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833:
829:
822:
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790:
786:
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771:
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763:
759:
755:
751:
747:
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743:
735:
732:
727:
723:
718:
713:
708:
703:
699:
695:
691:
687:
686:
681:
674:
671:
666:
659:
653:
651:
647:
642:
638:
634:
628:
624:
617:
614:
609:
605:
601:
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593:
589:
585:
581:
577:
573:
572:
564:
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558:
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537:
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529:
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521:
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516:
508:
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499:
497:
493:
490:
488:
483:
482:SNP calling.
479:
476:
472:
468:
463:
457:
450:
447:
444:
442:
439:
438:
435:Up to 500 GB
434:
432:Up to 360 GB
431:
428:
426:
423:
422:
418:
415:
413:Early Access
412:
410:
409:
403:
396:Up to 200 GB
395:
393:Up to 160 GB
392:
389:
387:
384:
383:
379:
376:
374:Early Access
373:
371:
370:
364:
357:
350:
347:
344:
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328:
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120:
118:
115:
112:
110:
102:
100:
98:
94:
93:DNA synthesis
90:
89:DNA synthesis
82:
80:
74:
72:
65:
63:
61:
57:
52:
48:
44:
40:
36:
32:
19:
2429:
2425:
2415:
2380:
2375:
2365:
2320:
2314:
2304:
2269:
2264:
2254:
2219:
2214:
2204:
2169:
2164:
2154:
2117:
2112:
2102:
2067:
2061:
2051:
2016:
2011:
2001:
1969:(6): 461–5.
1966:
1962:Nat. Methods
1960:
1950:
1913:
1908:
1898:
1855:
1849:
1839:
1784:
1781:Genome Biol.
1779:
1769:
1734:
1729:
1719:
1687:(7): 701–7.
1684:
1679:
1669:
1634:
1629:
1619:
1610:
1597:
1588:
1584:
1574:
1542:(1): 33–42.
1539:
1534:
1524:
1489:
1484:
1478:
1470:
1461:
1449:
1424:. Retrieved
1420:the original
1410:
1402:Bio-IT World
1401:
1392:
1383:
1374:
1365:
1356:
1347:
1335:
1323:. Retrieved
1319:the original
1315:Bio-IT World
1314:
1305:
1296:
1287:
1278:
1267:
1255:. Retrieved
1251:the original
1246:
1237:
1226:. Retrieved
1222:the original
1217:
1208:
1199:
1190:
1181:
1172:
1129:
1123:
1113:
1104:
1095:
1086:
1076:
1067:
1046:
1034:
1025:
1013:
1004:
992:
983:
971:
962:
950:
941:
884:
880:
870:
835:
831:
821:
788:
784:
778:
745:
740:
734:
689:
683:
673:
664:
622:
616:
575:
569:
519:
513:
507:
494:
491:
484:
480:
474:
466:
464:
461:
451:Up to 50 GB
448:Up to 36 GB
440:
424:
401:
385:
361:
340:
324:
300:
284:
281:
278:
275:
254:350M - 500M
251:250M - 300M
240:
229:4300 - 5000
226:2500 - 2900
218:
186:
183:
179:
175:
171:
168:
164:
153:RS and RS II
145:
132:
124:
116:
113:
109:nanophotonic
106:
86:
78:
69:
38:
34:
30:
29:
2070:(1): 75–7.
2063:Nat Methods
1787:(9): R101.
1631:Genome Res.
1384:PacBio Blog
1366:PacBio Blog
1325:16 November
1200:PacBio Blog
1105:PacBio Blog
1068:PacBio Blog
458:Application
248:60M - 100M
2486:Categories
2019:(4): e29.
1916:(1): 375.
1910:BMC Genom.
1426:2018-09-24
1228:2012-04-28
500:References
487:methylated
351:20B - 50B
348:10B - 20B
257:500M - 1B
245:30M - 40M
66:Technology
56:nucleotide
39:sequencing
2120:(1): 10.
1794:1304.3752
1462:GenomeWeb
1348:GenomeWeb
1279:GenomeWeb
1047:GenomeWeb
1026:GenomeWeb
1005:GenomeWeb
984:GenomeWeb
963:GenomeWeb
805:1936-122X
641:813987819
390:~67.4 GB
2502:Genomics
2456:28921562
2407:29401301
2357:24282307
2296:24108091
2246:23138224
2196:23034806
2146:22185597
2094:22101853
2043:22156058
1993:20453866
1942:22863213
1890:22504184
1831:24034426
1761:22750884
1711:22750883
1661:22829535
1566:21142692
1516:21793740
1435:cite web
1257:18 April
1164:25383537
921:29427429
862:36132755
813:22577821
770:38892226
726:18216253
608:54488479
600:19023044
544:12560545
429:~250 GB
345:5B - 8B
288:amplicon
128:Illumina
2447:5725701
2398:5861413
2348:3864310
2325:Bibcode
2287:4075632
2237:3879109
2187:3526280
2137:3264494
2085:3646335
2034:3287169
1984:2879396
1933:3443046
1881:3390926
1860:Bibcode
1822:4053942
1799:Bibcode
1752:3707490
1702:3731737
1652:3483556
1557:3030187
1507:3168948
1479:E. coli
1297:Twitter
1155:4317254
1134:Bibcode
942:Twitter
912:5861821
889:Bibcode
853:9417158
750:Bibcode
717:2234111
694:Bibcode
667:. 2008.
580:Bibcode
571:Science
552:6060239
524:Bibcode
515:Science
475:de novo
467:de novo
445:~25 GB
358:8M Chip
187:de novo
142:History
2454:
2444:
2405:
2395:
2355:
2345:
2294:
2284:
2244:
2234:
2194:
2184:
2144:
2134:
2092:
2082:
2041:
2031:
1991:
1981:
1940:
1930:
1888:
1878:
1851:Nature
1829:
1819:
1759:
1749:
1709:
1699:
1659:
1649:
1564:
1554:
1514:
1504:
1162:
1152:
1125:Nature
919:
909:
860:
850:
811:
803:
768:
724:
714:
639:
629:
606:
598:
550:
542:
264:Sequel
213:P6-C4
210:P5-C3
207:P4-XL
1789:arXiv
1607:(PDF)
766:S2CID
661:(PDF)
604:S2CID
548:S2CID
232:8500
223:1100
2452:PMID
2403:PMID
2353:PMID
2316:PNAS
2292:PMID
2242:PMID
2192:PMID
2142:PMID
2090:PMID
2039:PMID
1989:PMID
1938:PMID
1886:PMID
1827:PMID
1757:PMID
1707:PMID
1657:PMID
1591:(8).
1562:PMID
1512:PMID
1441:link
1327:2015
1259:2013
1160:PMID
917:PMID
858:PMID
809:PMID
801:ISSN
722:PMID
685:PNAS
637:OCLC
627:ISBN
596:PMID
540:PMID
465:For
419:2.0
416:1.0
380:2.0
377:1.0
319:6.0
316:2.1
35:SMRT
2442:PMC
2434:doi
2393:PMC
2385:doi
2343:PMC
2333:doi
2321:110
2282:PMC
2274:doi
2232:PMC
2224:doi
2182:PMC
2174:doi
2132:PMC
2122:doi
2080:PMC
2072:doi
2029:PMC
2021:doi
1979:PMC
1971:doi
1928:PMC
1918:doi
1876:PMC
1868:doi
1856:485
1817:PMC
1807:doi
1747:PMC
1739:doi
1697:PMC
1689:doi
1647:PMC
1639:doi
1552:PMC
1544:doi
1540:364
1502:PMC
1494:doi
1490:365
1150:PMC
1142:doi
1130:517
907:PMC
897:doi
848:PMC
840:doi
793:doi
758:doi
746:103
712:PMC
702:doi
690:105
588:doi
576:323
532:doi
520:299
313:V2
204:C2
201:C1
2488::
2450:.
2440:.
2430:37
2428:.
2424:.
2401:.
2391:.
2381:46
2374:.
2351:.
2341:.
2331:.
2319:.
2313:.
2290:.
2280:.
2270:31
2263:.
2240:.
2230:.
2220:30
2213:.
2190:.
2180:.
2170:40
2163:.
2140:.
2130:.
2111:.
2088:.
2078:.
2066:.
2060:.
2037:.
2027:.
2017:40
2010:.
1987:.
1977:.
1965:.
1959:.
1936:.
1926:.
1914:13
1907:.
1884:.
1874:.
1866:.
1854:.
1848:.
1825:.
1815:.
1805:.
1797:.
1785:14
1778:.
1755:.
1745:.
1735:30
1728:.
1705:.
1695:.
1685:30
1678:.
1655:.
1645:.
1635:22
1628:.
1609:.
1589:32
1587:.
1583:.
1560:.
1550:.
1533:.
1510:.
1500:.
1483:.
1460:.
1437:}}
1433:{{
1400:.
1382:.
1364:.
1346:.
1313:.
1295:.
1277:.
1245:.
1216:.
1198:.
1180:.
1158:.
1148:.
1140:.
1128:.
1122:.
1103:.
1085:.
1066:.
1055:^
1045:.
1024:.
1003:.
982:.
961:.
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