376:
because CD48 is a ligand for CD244, and both would be positive only in the activated lineage-restricted progenitors. It seems that this code was more efficient than the more tedious earlier set of the large number of markers, and are also conserved across the mouse strains; however, recent work has
410:
was the first to isolate mouse hematopoietic stem cells in 1986 and was also the first to work out the markers to distinguish the mouse long-term (LT-HSC) and short-term (ST-HSC) hematopoietic stem cells (self-renew-capable), and the Multi-potent progenitors (MPP, low or no self-renew capability –
191:
Traditional purification method used to yield a reasonable purity level of mouse hematopoietic stem cells, in general, requires a large (~10–12) battery of markers, most of which were surrogate markers with little functional significance, and thus partial overlap with the stem cell populations and
28:
where the combination of several different cell surface markers is used to separate the rare HSCs from the surrounding blood cells. HSCs lack expression of mature blood cell markers and are thus, called Lin-. Lack of expression of lineage markers is used in combination with detection of several
218:) family is a group of more than 10 molecules whose genes are located mostly tandemly in a single locus on chromosome 1 (mouse), all belonging to a subset of the immunoglobulin gene superfamily, and originally thought to be involved in T-cell stimulation. This family includes
622:
Tindle RW, Nichols RA, Chan L, Campana D, Catovsky D, Birnie GD (1985). "A novel monoclonal antibody BI-3C5 recognises myeloblasts and non-B non-T lymphoblasts in acute leukaemias and CGL blast crises, and reacts with immature cells in normal bone marrow".
56:
The classical marker of human HSC is CD34 first described independently by Civin et al. and Tindle et al. It is used to isolate HSC for reconstitution of patients who are haematologically incompetent as a result of chemotherapy or disease.
979:
Muller-Sieburg, Christa E; Whitlock, Cheryl A; Weissman, Irving L (1986). "Isolation of two early B lymphocyte progenitors from mouse marrow: A committed Pre-Pre-B cell and a clonogenic Thy-1lo hematopoietic stem cell".
594:
Civin CI. Strauss LC. Brovall C. Fackler MJ. Schwartz JF. Shaper JH. (1984). "Antigenic analysis of haematopoiesis:III. hematopoietic cell surface antigen defined by a monoclonal antibody raised against KG-1a cells".
29:
positive cell-surface markers to isolate HSCs. In addition, HSCs are characterized by their small size and low staining with vital dyes such as rhodamine 123 (rhodamine ) or
Hoechst 33342 (side population).
658:
Tindle RW, Katz F, Martin H, Watt S, Catovsky D, Janossy G, Greaves M (1987). "BI-3C5 (CD34) defines multipotential and lineage restricted progenitor cells and their leukaemic counterparts".
208:
Alternative methods that could give rise to a similar or better harvest of stem cells is an active area of research, and are presently emerging. One such method uses a signature of
1017:"The origins of the identification and isolation of hematopoietic stem cells, and their capability to induce donor-specific transplantation tolerance and treat autoimmune diseases"
164:
However, not all stem cells are covered by these combinations that, nonetheless, have become popular. In fact, even in humans, there are hematopoietic stem cells that are
797:
Doi H, Inaba M, Yamamoto Y, Taketani S, Mori SI, Sugihara A, Ogata H, Toki J, Hisha H, Inaba K, Sogo S, Adachi M, Matsuda T, Good RA, Ikehara S (March 1997).
215:
210:
1016:
99:
There are many differences between the human and murine hematopoietic cell markers for the commonly accepted type of hematopoietic stem cells.
45:
Manually by culture. Since CD34 are in suspension culture and almost all cells in PBMC gets adhered, CD34 can be isolated through this process
673:
Loken MR, Shah VO, Civin CI (1987). "Characterization of myeloid antigens on human bone marrow using multicolour immunofluorescence".
338:
317:
688:
718:
411:
the later the developmental stage of MPP, the lesser the self-renewal ability and the more of some of the markers like CD4 and
20:
cannot be isolated as a pure population, it is not possible to identify them under a microscope. Therefore, there are many
940:"SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells"
278:
172:. Also some later studies suggested that earliest stem cells may lack c-kit on the cell surface. For human HSCs use of
1070:
61:
858:"CD150- side population cells represent a functionally distinct population of long-term hematopoietic stem cells"
296:
260:
377:
shown that this method excludes a large number of HSCs and includes an equally large number of non-stem cells.
242:
17:
230:, etc., CD150 being the founding member, and, thus, also known as slamF1, i.e., SLAM family member 1.
810:
407:
192:
sometimes other closely related cells that are not stem cells. Also, some of these markers (e.g.,
1046:
997:
961:
887:
838:
779:
738:
640:
1036:
1028:
989:
951:
918:
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769:
730:
632:
604:
93:
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517:
480:
443:
403:
814:
1041:
882:
857:
719:"A newly discovered class of human hematopoietic cells with SCID-repopulating activity"
696:
25:
1064:
993:
833:
798:
636:
32:
CD34+ Cells can be isolated by 4 different techniques from peripheral blood samples
1032:
873:
938:
Kiel MJ, Yilmaz OH, Iwashita T, Yilmaz OH, Terhorst C, Morrison SJ (July 2005).
956:
939:
923:
906:
803:
Proceedings of the
National Academy of Sciences of the United States of America
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774:
757:
1050:
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891:
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332:
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758:"CD34- hematopoietic stem cells: current concepts and controversies"
570:
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223:
196:) are not conserved across mouse species, and use of markers like
185:
173:
157:
127:
115:
89:
81:
77:
734:
717:
Bhatia M, Bonnet D, Murdoch B, Gan OI, Dick JE (September 1998).
856:
Weksberg DC, Chambers SM, Boles NC, Goodell MA (February 2008).
550:
542:
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111:
107:
85:
73:
69:
65:
200:
for HSC purification requires mice to be at least 8 weeks old.
574:
533:
675:
660:
24:(HSCs). HSCs can be identified or isolated by the use of
799:"Pluripotent hemopoietic stem cells are c-kit<low"
22:techniques to isolate haematopoietic stem cells
1015:Weissman, Irving L; Shizuru, Judith A (2008).
8:
52:Cluster of differentiation and other markers
1040:
955:
922:
881:
832:
822:
773:
214:family cell surface molecules. The SLAM (
216:Signaling lymphocyte activation molecule
756:Guo Y, Lübbert M, Engelhardt M (2003).
586:
7:
384:gave stem cell purity comparable to
279:Lineage-restricted progenitor cells
237:for the hemopoietic hierarchy are:
907:"Stem cell markers: less is more!"
339:Megakaryocyte-erythroid progenitor
14:
318:Granulocyte-macrophage progenitor
399:LT-HSC/ST-HSC/early MPP/late MPP
1:
1053:– via ASH Publications.
1033:10.1182/blood-2008-08-078220
994:10.1016/0092-8674(86)90274-6
874:10.1182/blood-2007-09-115006
637:10.1016/0145-2126(85)90016-5
261:Multipotent progenitor cells
176:was one step ahead as both
60:Many markers belong to the
42:By labelled anti-antibodies
36:By magnetic beads with MACS
1087:
957:10.1016/j.cell.2005.05.026
924:10.1182/blood-2005-11-4400
609:10.4049/jimmunol.133.1.157
366:was sufficient instead of
62:cluster of differentiation
775:10.1634/stemcells.21-1-15
297:Common myeloid progenitor
18:haematopoietic stem cells
243:Hematopoietic stem cells
689:"Stemcells Redirection"
824:10.1073/pnas.94.6.2513
597:Journal of Immunology
905:Van Zant G (2005).
815:1997PNAS...94.2513D
408:Stanford University
92:– the receptor for
1071:Stem cell research
677:. pp. 630–35.
693:stemcells.nih.gov
625:Leukemia Research
1078:
1055:
1054:
1044:
1027:(9): 3543–3553.
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747:
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695:. Archived from
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679:
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649:
648:
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613:
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94:stem cell factor
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723:Nature Medicine
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404:Irving Weissman
401:
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54:
12:
11:
5:
1084:
1082:
1074:
1073:
1063:
1062:
1057:
1056:
1007:
988:(4): 653–662.
971:
950:(7): 1109–21.
930:
897:
868:(4): 2444–51.
848:
789:
748:
729:(9): 1038–45.
709:
680:
665:
650:
614:
585:
584:
582:
579:
578:
577:
536:
495:
454:
400:
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357:
356:
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314:
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233:The signature
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202:
162:
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131:
64:series, like:
53:
50:
49:
48:
46:
43:
40:
37:
26:flow cytometry
13:
10:
9:
6:
4:
3:
2:
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1048:
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1038:
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972:
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958:
953:
949:
945:
941:
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931:
925:
920:
917:(3): 855–56.
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893:
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884:
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875:
871:
867:
863:
859:
852:
849:
844:
840:
835:
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825:
820:
816:
812:
809:(6): 2513–7.
808:
804:
800:
793:
790:
785:
781:
776:
771:
767:
763:
759:
752:
749:
744:
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699:on 2011-05-25
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661:
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618:
615:
610:
606:
603:(1): 157–65.
602:
598:
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587:
580:
576:
572:
571:Mac-1 (CD11b)
568:
564:
560:
556:
552:
548:
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540:
537:
535:
531:
530:Mac-1 (CD11b)
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493:Mac-1 (CD11b)
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19:
1024:
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933:
914:
910:
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851:
806:
802:
792:
768:(1): 15–20.
765:
761:
751:
735:10.1038/2023
726:
722:
712:
701:. Retrieved
697:the original
692:
683:
674:
668:
659:
653:
628:
624:
617:
600:
596:
589:
567:Slamf1/CD150
538:
526:Slamf1/CD150
497:
489:Slamf1/CD150
456:
452:Slamf1/CD150
419:
406:'s group at
402:
358:
337:
316:
295:
277:
259:
241:
234:
232:
209:
207:
190:
163:
133:
103:
98:
59:
55:
31:
21:
15:
158:C-kit/CD117
88:, and also
762:Stem Cells
703:2019-07-08
631:(1): 1–9.
581:References
359:For HSCs,
235:SLAM codes
184:HSCs were
662:: 654–55.
498:Early MPP
395:in mice.
204:SLAM code
150:Thy1/CD90
134:Human HSC
104:Mouse HSC
1065:Category
1051:18948588
966:15989959
892:18055867
784:12529547
539:Late MPP
1042:2574516
1002:2868799
883:2234069
843:9122226
811:Bibcode
743:9734397
645:3857402
354:CD16/32
333:CD16/32
312:CD16/32
39:By FACS
1049:
1039:
1000:
964:
890:
880:
841:
831:
782:
741:
643:
551:Thy1.1
510:Thy1.1
473:Thy1.1
457:ST-HSC
436:Thy1.1
420:LT-HSC
281:(LRPs)
263:(MPPs)
120:Thy1.1
16:Since
1021:Blood
911:Blood
862:Blood
834:20119
563:CD135
555:C-kit
547:SCA-1
522:CD135
514:C-kit
506:SCA-1
485:CD135
477:C-kit
469:SCA-1
448:CD135
440:C-kit
432:SCA-1
413:CD135
393:c-kit
389:SCA-1
379:CD150
374:CD244
368:CD150
361:CD150
348:c-kit
345:SCA-1
343:: lin
341:(MEP)
327:c-kit
324:SCA-1
322:: lin
320:(GMP)
306:c-kit
303:SCA-1
301:: lin
299:(CMP)
291:CD244
285:CD150
273:CD244
267:CD150
255:CD244
249:CD150
245:(HSC)
228:CD244
224:CD150
186:CD133
174:CD133
160:, lin
130:, lin
128:C-kit
116:SCA-1
90:c-kit
82:CD105
78:CD133
1047:PMID
998:PMID
982:Cell
962:PMID
944:Cell
888:PMID
839:PMID
780:PMID
739:PMID
641:PMID
543:CD34
502:CD34
465:CD38
461:CD34
428:CD38
424:CD34
386:Thy1
382:CD48
371:CD48
364:CD48
351:CD34
330:CD34
309:CD34
288:CD48
270:CD48
252:CD48
220:CD48
211:SLAM
198:CD34
194:Thy1
182:CD34
180:and
178:CD34
170:CD38
166:CD34
154:CD38
146:CD59
142:CD34
138:EMCN
124:CD38
112:CD34
108:EMCN
86:CD45
74:CD90
70:CD38
66:CD34
1037:PMC
1029:doi
1025:112
990:doi
952:doi
948:121
919:doi
915:107
878:PMC
870:doi
866:111
829:PMC
819:doi
770:doi
731:doi
633:doi
605:doi
601:133
575:CD4
559:lin
534:CD4
518:lin
481:lin
444:lin
415:):
391:lin
1067::
1045:.
1035:.
1023:.
1019:.
996:.
986:44
984:.
960:.
946:.
942:.
913:.
909:.
886:.
876:.
864:.
860:.
837:.
827:.
817:.
807:94
805:.
801:.
778:.
766:21
764:.
760:.
737:.
725:.
721:.
691:.
639:.
627:.
599:.
573:,
569:,
565:,
561:,
557:,
553:,
549:,
545:,
541::
532:,
528:,
524:,
520:,
516:,
512:,
508:,
504:,
500::
491:,
487:,
483:,
479:,
475:,
471:,
467:,
463:,
459::
450:,
446:,
442:,
438:,
434:,
430:,
426:,
422::
283::
265::
247::
226:,
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188:.
156:,
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96:.
84:,
80:,
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72:,
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1004:.
992::
968:.
954::
927:.
921::
894:.
872::
845:.
821::
813::
786:.
772::
745:.
733::
727:4
706:.
647:.
635::
629:9
611:.
607::
168:/
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