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Thioglycolate broth

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85: 959: 20: 77:. This, combined with the diffusion of oxygen from the top of the broth, produces a range of oxygen concentrations in the medium along its depth. The oxygen concentration at a given level is indicated by a redox-sensitive dye such as 45:
can grow with or without oxygen because they can metabolise energy aerobically or anaerobically. They gather mostly at the top because aerobic respiration generates more ATP than either fermentation or anaerobic respiration.
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need oxygen because they cannot ferment or respire anaerobically. However, they are poisoned by high concentrations of oxygen. They gather in the upper part of the test tube, but not the very top.
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do not require oxygen as they metabolise energy anaerobically. Unlike obligate anaerobes, though, they are not poisoned by oxygen. They can be found evenly spread throughout the test tube.
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need oxygen because they cannot ferment or respire anaerobically. They gather at the top of the tube where the oxygen concentration is highest.
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are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.
640: 626: 620: 494: 308: 55: 843: 914: 817: 355: 347: 342: 337: 264: 42: 792: 904: 894: 802: 744: 739: 727: 179: 154: 416: 868: 848: 567: 523: 503: 66: 449: 606: 555: 535: 468: 113: 89: 873: 473: 402: 243: 183: 134: 130: 101: 74: 36: 214:"Effect of thioglycolate on phagocytic and microbicidal activities of peritoneal macrophages" 938: 863: 749: 454: 233: 225: 97: 30: 388: 361: 970: 853: 838: 807: 718: 646: 462: 317: 158: 109: 49: 238: 213: 1013: 858: 782: 703: 518: 410: 299: 287: 70: 966: 396: 229: 173: 429: 118: 833: 797: 769: 673: 654: 592: 291: 126: 78: 69:
used primarily to determine the oxygen requirements of microorganisms. Sodium
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can be identified by growing them in test tubes of thioglycolate broth:
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Leijh PC; van Zwet TL; ter Kuile MN; van Furth R (November 1984).
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species will be seen growing only in the bottom of the test tube.
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Thioglycollate broth medium is recommended to isolate strict
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District Laboratory Practice in Tropical Countries, Part 2
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in the medium consumes oxygen and permits the growth of
974: 887: 826: 768: 702: 566: 534: 502: 493: 372: 316: 307: 298: 994: 272: 8: 125:Thioglycolate broth is also used to recruit 92:should an anaerobic infection be suspected. 81:that turns pink in the presence of oxygen. 1001: 987: 900:Cystine–lactose–electrolyte-deficient agar 499: 313: 304: 279: 265: 257: 155:"Fluid Thioglycollate [sic] Broth" 237: 584:Tryptic soy-serum-bacitracin-vancomycin 207: 205: 145: 577:Aggregatibacter actinomycetemcomitans 116:. For example, obligately anaerobic 7: 955: 953: 633:Buffered charcoal yeast extract agar 96:This allows the differentiation of 16:Culture medium used in microbiology 14: 957: 713:Lactose fermenting gram negative 65:is a multipurpose, enrichment, 1: 230:10.1128/iai.46.2.448-452.1984 973:. You can help Knowledge by 172:Cheesbrough, Monica (2006). 383:Corynebacterium diphtheriae 1046: 952: 327:Mycobacterium tuberculosis 920:Mannitol motility medium 788:Dermatophyte test medium 333:Löwenstein–Jensen medium 910:Glucose phosphate broth 723:Sorbitol-MacConkey agar 969:-related article is a 641:Pseudomonas aeruginosa 627:Legionella pneumophila 621:Haemophilus influenzae 218:Infection and Immunity 133:of mice when injected 114:aerotolerant organisms 93: 59: 56:Aerotolerant organisms 23:Aerobic and anaerobic 1020:Microbiological media 915:Lauryl tryptose broth 888:Other/ungrouped media 755:Simmons' citrate agar 356:Mycoplasma pneumoniae 348:Middlebrook 7H11 Agar 343:Middlebrook 7H10 Agar 338:Middlebrook 7H9 Broth 106:facultative anaerobes 87: 43:Facultative anaerobes 22: 905:Cystine tryptic agar 895:Brain heart infusion 879:Zobell's marine agar 803:Potato dextrose agar 745:Hektoen enteric agar 740:Bismuth sulfite agar 728:Eosin methylene blue 182:Press. p. 126. 180:Cambridge University 925:Mueller–Hinton agar 869:Trypticase soy agar 844:Columbia blood agar 568:Gammaproteobacteria 504:Alphaproteobacteria 67:differential medium 63:Thioglycolate broth 1030:Microbiology stubs 1025:Cell culture media 827:Nonselective media 704:Differential media 607:Enterobacteriaceae 599:Bordet–Gengou agar 556:New York City agar 551:Thayer–Martin agar 536:Betaproteobacteria 479:Vogel–Johnson agar 469:Mannitol salt agar 102:obligate anaerobes 94: 75:obligate anaerobes 60: 37:Obligate anaerobes 982: 981: 947: 946: 874:Tryptic soy broth 793:Malt extract agar 698: 697: 694: 693: 489: 488: 474:Baird-Parker agar 403:Bile esculin agar 135:intraperitoneally 131:peritoneal cavity 1037: 1003: 996: 989: 961: 954: 864:Plate count agar 750:Lysine iron agar 524:Farrell's medium 500: 314: 305: 281: 274: 267: 258: 252: 251: 241: 209: 200: 199: 197: 196: 169: 163: 162: 150: 98:obligate aerobes 31:Obligate aerobes 1045: 1044: 1040: 1039: 1038: 1036: 1035: 1034: 1010: 1009: 1008: 1007: 950: 948: 943: 883: 822: 764: 690: 562: 530: 485: 368: 300:Selective media 294: 285: 255: 211: 210: 203: 194: 192: 190: 171: 170: 166: 152: 151: 147: 143: 110:microaerophiles 53: 50:Microaerophiles 47: 40: 34: 28: 17: 12: 11: 5: 1043: 1041: 1033: 1032: 1027: 1022: 1012: 1011: 1006: 1005: 998: 991: 983: 980: 979: 962: 945: 944: 942: 941: 936: 931: 922: 917: 912: 907: 902: 897: 891: 889: 885: 884: 882: 881: 876: 871: 866: 861: 856: 854:Lysogeny broth 851: 846: 841: 839:Chocolate agar 836: 830: 828: 824: 823: 821: 820: 815: 810: 808:Sabouraud agar 805: 800: 795: 790: 785: 780: 774: 772: 766: 765: 763: 762: 757: 752: 747: 742: 737: 736: 735: 730: 725: 719:MacConkey agar 708: 706: 700: 699: 696: 695: 692: 691: 689: 688: 687: 686: 670: 665: 664: 663: 651: 650: 649: 647:Cetrimide agar 637: 636: 635: 617: 616: 615: 603: 602: 601: 589: 588: 587: 572: 570: 564: 563: 561: 560: 559: 558: 553: 540: 538: 532: 531: 529: 528: 527: 526: 521: 508: 506: 497: 491: 490: 487: 486: 484: 483: 482: 481: 476: 471: 463:Staphylococcus 459: 458: 457: 452: 440: 439: 438: 426: 425: 424: 419: 407: 406: 405: 393: 392: 391: 378: 376: 370: 369: 367: 366: 365: 364: 352: 351: 350: 345: 340: 335: 322: 320: 318:Actinomycetota 311: 302: 296: 295: 286: 284: 283: 276: 269: 261: 254: 253: 224:(2): 448–452. 201: 188: 164: 159:Collin College 144: 142: 139: 15: 13: 10: 9: 6: 4: 3: 2: 1042: 1031: 1028: 1026: 1023: 1021: 1018: 1017: 1015: 1004: 999: 997: 992: 990: 985: 984: 978: 976: 972: 968: 963: 960: 956: 951: 940: 939:Schädler agar 937: 935: 932: 930: 926: 923: 921: 918: 916: 913: 911: 908: 906: 903: 901: 898: 896: 893: 892: 890: 886: 880: 877: 875: 872: 870: 867: 865: 862: 860: 859:Nutrient agar 857: 855: 852: 850: 849:Letheen broth 847: 845: 842: 840: 837: 835: 832: 831: 829: 825: 819: 816: 814: 811: 809: 806: 804: 801: 799: 796: 794: 791: 789: 786: 784: 783:Czapek medium 781: 779: 776: 775: 773: 771: 767: 761: 758: 756: 753: 751: 748: 746: 743: 741: 738: 734: 731: 729: 726: 724: 720: 717: 716: 715: 714: 710: 709: 707: 705: 701: 685: 682: 681: 680: 679: 675: 671: 669: 666: 662: 659: 658: 657: 656: 652: 648: 645: 644: 643: 642: 638: 634: 631: 630: 629: 628: 623: 622: 618: 614: 611: 610: 609: 608: 604: 600: 597: 596: 595: 594: 590: 586: 585: 581: 580: 579: 578: 574: 573: 571: 569: 565: 557: 554: 552: 549: 548: 547: 546: 542: 541: 539: 537: 533: 525: 522: 520: 519:Brucella agar 517: 516: 515: 514: 510: 509: 507: 505: 501: 498: 496: 495:Gram negative 492: 480: 477: 475: 472: 470: 467: 466: 465: 464: 460: 456: 453: 451: 448: 447: 446: 445: 441: 437: 434: 433: 432: 431: 427: 423: 420: 418: 415: 414: 413: 412: 411:Lactobacillus 408: 404: 401: 400: 399: 398: 394: 390: 387: 386: 385: 384: 380: 379: 377: 375: 371: 363: 360: 359: 358: 357: 353: 349: 346: 344: 341: 339: 336: 334: 331: 330: 329: 328: 324: 323: 321: 319: 315: 312: 310: 309:Gram positive 306: 303: 301: 297: 293: 289: 282: 277: 275: 270: 268: 263: 262: 259: 249: 245: 240: 235: 231: 227: 223: 219: 215: 208: 206: 202: 191: 189:0-521-67631-2 185: 181: 177: 176: 168: 165: 160: 156: 153:Cain, Donna. 149: 146: 140: 138: 136: 132: 128: 123: 121: 120: 115: 111: 107: 103: 99: 91: 86: 82: 80: 76: 72: 71:thioglycolate 68: 64: 57: 51: 44: 38: 32: 26: 21: 975:expanding it 967:microbiology 964: 949: 770:Fungal media 711: 672: 653: 639: 625: 619: 605: 591: 582: 575: 543: 511: 461: 450:Fraser broth 442: 428: 409: 397:Enterococcus 395: 389:Hoyle's agar 381: 362:Eaton's agar 354: 325: 288:Growth media 221: 217: 193:. Retrieved 174: 167: 148: 124: 117: 95: 62: 61: 430:Lactococcus 292:agar plates 127:macrophages 119:Clostridium 1014:Categories 834:Blood agar 798:MMN medium 674:Salmonella 655:Salmonella 593:Bordetella 417:Lee's Agar 195:2009-09-14 141:References 79:resazurine 760:TSI slant 733:Endo agar 613:VRBD agar 545:Neisseria 374:Bacillota 90:anaerobes 934:R2A agar 929:PNP agar 778:BAF agar 684:XLD agar 678:Shigella 668:DCA agar 661:XLT agar 513:Brucella 444:Listeria 436:M17 agar 422:MRS agar 25:bacteria 248:6500699 129:to the 455:PALCAM 246:  239:261553 236:  186:  112:, and 965:This 971:stub 813:YEPD 244:PMID 184:ISBN 234:PMC 226:doi 54:5: 48:4: 41:3: 35:2: 29:1: 1016:: 818:YM 290:/ 242:. 232:. 222:46 220:. 216:. 204:^ 178:. 157:. 108:, 104:, 100:, 1002:e 995:t 988:v 977:. 927:/ 721:/ 676:/ 624:/ 280:e 273:t 266:v 250:. 228:: 198:. 161:.

Index


bacteria
Obligate aerobes
Obligate anaerobes
Facultative anaerobes
Microaerophiles
Aerotolerant organisms
differential medium
thioglycolate
obligate anaerobes
resazurine

anaerobes
obligate aerobes
obligate anaerobes
facultative anaerobes
microaerophiles
aerotolerant organisms
Clostridium
macrophages
peritoneal cavity
intraperitoneally
"Fluid Thioglycollate [sic] Broth"
Collin College
District Laboratory Practice in Tropical Countries, Part 2
Cambridge University
ISBN
0-521-67631-2

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