Knowledge (XXG)

Thioglycolate broth

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96: 970: 31: 88:. This, combined with the diffusion of oxygen from the top of the broth, produces a range of oxygen concentrations in the medium along its depth. The oxygen concentration at a given level is indicated by a redox-sensitive dye such as 56:
can grow with or without oxygen because they can metabolise energy aerobically or anaerobically. They gather mostly at the top because aerobic respiration generates more ATP than either fermentation or anaerobic respiration.
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need oxygen because they cannot ferment or respire anaerobically. However, they are poisoned by high concentrations of oxygen. They gather in the upper part of the test tube, but not the very top.
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do not require oxygen as they metabolise energy anaerobically. Unlike obligate anaerobes, though, they are not poisoned by oxygen. They can be found evenly spread throughout the test tube.
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need oxygen because they cannot ferment or respire anaerobically. They gather at the top of the tube where the oxygen concentration is highest.
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are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.
651: 637: 631: 505: 319: 66: 854: 925: 828: 366: 358: 353: 348: 275: 53: 803: 915: 905: 813: 755: 750: 738: 190: 165: 427: 879: 859: 578: 534: 514: 77: 460: 617: 566: 546: 479: 124: 100: 884: 484: 413: 254: 194: 145: 141: 112: 85: 47: 981: 225:"Effect of thioglycolate on phagocytic and microbicidal activities of peritoneal macrophages" 949: 874: 760: 465: 244: 236: 108: 41: 399: 372: 864: 849: 818: 729: 657: 473: 328: 169: 120: 60: 249: 224: 1024: 869: 793: 714: 529: 421: 310: 298: 81: 17: 977: 407: 240: 184: 440: 129: 844: 808: 780: 684: 665: 603: 302: 137: 89: 80:
used primarily to determine the oxygen requirements of microorganisms. Sodium
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can be identified by growing them in test tubes of thioglycolate broth:
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Leijh PC; van Zwet TL; ter Kuile MN; van Furth R (November 1984).
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species will be seen growing only in the bottom of the test tube.
94: 29: 823: 148:. It recruits numerous macrophages, but does not activate them. 271: 99:
Thioglycollate broth medium is recommended to isolate strict
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District Laboratory Practice in Tropical Countries, Part 2
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in the medium consumes oxygen and permits the growth of
985: 898: 837: 779: 713: 577: 545: 513: 504: 383: 327: 318: 309: 1005: 283: 8: 136:Thioglycolate broth is also used to recruit 103:should an anaerobic infection be suspected. 92:that turns pink in the presence of oxygen. 1012: 998: 911:Cystine–lactose–electrolyte-deficient agar 510: 324: 315: 290: 276: 268: 166:"Fluid Thioglycollate [sic] Broth" 248: 595:Tryptic soy-serum-bacitracin-vancomycin 218: 216: 156: 588:Aggregatibacter actinomycetemcomitans 127:. For example, obligately anaerobic 7: 966: 964: 644:Buffered charcoal yeast extract agar 107:This allows the differentiation of 27:Culture medium used in microbiology 984:. You can help Knowledge (XXG) by 25: 968: 724:Lactose fermenting gram negative 76:is a multipurpose, enrichment, 1: 241:10.1128/iai.46.2.448-452.1984 183:Cheesbrough, Monica (2006). 394:Corynebacterium diphtheriae 1057: 963: 338:Mycobacterium tuberculosis 931:Mannitol motility medium 799:Dermatophyte test medium 344:Löwenstein–Jensen medium 921:Glucose phosphate broth 734:Sorbitol-MacConkey agar 980:-related article is a 652:Pseudomonas aeruginosa 638:Legionella pneumophila 632:Haemophilus influenzae 229:Infection and Immunity 144:of mice when injected 125:aerotolerant organisms 104: 70: 67:Aerotolerant organisms 34:Aerobic and anaerobic 1031:Microbiological media 926:Lauryl tryptose broth 899:Other/ungrouped media 766:Simmons' citrate agar 367:Mycoplasma pneumoniae 359:Middlebrook 7H11 Agar 354:Middlebrook 7H10 Agar 349:Middlebrook 7H9 Broth 117:facultative anaerobes 98: 54:Facultative anaerobes 33: 916:Cystine tryptic agar 906:Brain heart infusion 890:Zobell's marine agar 814:Potato dextrose agar 756:Hektoen enteric agar 751:Bismuth sulfite agar 739:Eosin methylene blue 193:Press. p. 126. 191:Cambridge University 18:Thioglycollate broth 936:Mueller–Hinton agar 880:Trypticase soy agar 855:Columbia blood agar 579:Gammaproteobacteria 515:Alphaproteobacteria 78:differential medium 74:Thioglycolate broth 1041:Microbiology stubs 1036:Cell culture media 838:Nonselective media 715:Differential media 618:Enterobacteriaceae 610:Bordet–Gengou agar 567:New York City agar 562:Thayer–Martin agar 547:Betaproteobacteria 490:Vogel–Johnson agar 480:Mannitol salt agar 113:obligate anaerobes 105: 86:obligate anaerobes 71: 48:Obligate anaerobes 993: 992: 958: 957: 885:Tryptic soy broth 804:Malt extract agar 709: 708: 705: 704: 500: 499: 485:Baird-Parker agar 414:Bile esculin agar 146:intraperitoneally 142:peritoneal cavity 16:(Redirected from 1048: 1014: 1007: 1000: 972: 965: 875:Plate count agar 761:Lysine iron agar 535:Farrell's medium 511: 325: 316: 292: 285: 278: 269: 263: 262: 252: 220: 211: 210: 208: 207: 180: 174: 173: 161: 109:obligate aerobes 42:Obligate aerobes 21: 1056: 1055: 1051: 1050: 1049: 1047: 1046: 1045: 1021: 1020: 1019: 1018: 961: 959: 954: 894: 833: 775: 701: 573: 541: 496: 379: 311:Selective media 305: 296: 266: 222: 221: 214: 205: 203: 201: 182: 181: 177: 163: 162: 158: 154: 121:microaerophiles 64: 61:Microaerophiles 58: 51: 45: 39: 28: 23: 22: 15: 12: 11: 5: 1054: 1052: 1044: 1043: 1038: 1033: 1023: 1022: 1017: 1016: 1009: 1002: 994: 991: 990: 973: 956: 955: 953: 952: 947: 942: 933: 928: 923: 918: 913: 908: 902: 900: 896: 895: 893: 892: 887: 882: 877: 872: 867: 865:Lysogeny broth 862: 857: 852: 850:Chocolate agar 847: 841: 839: 835: 834: 832: 831: 826: 821: 819:Sabouraud agar 816: 811: 806: 801: 796: 791: 785: 783: 777: 776: 774: 773: 768: 763: 758: 753: 748: 747: 746: 741: 736: 730:MacConkey agar 719: 717: 711: 710: 707: 706: 703: 702: 700: 699: 698: 697: 681: 676: 675: 674: 662: 661: 660: 658:Cetrimide agar 648: 647: 646: 628: 627: 626: 614: 613: 612: 600: 599: 598: 583: 581: 575: 574: 572: 571: 570: 569: 564: 551: 549: 543: 542: 540: 539: 538: 537: 532: 519: 517: 508: 502: 501: 498: 497: 495: 494: 493: 492: 487: 482: 474:Staphylococcus 470: 469: 468: 463: 451: 450: 449: 437: 436: 435: 430: 418: 417: 416: 404: 403: 402: 389: 387: 381: 380: 378: 377: 376: 375: 363: 362: 361: 356: 351: 346: 333: 331: 329:Actinomycetota 322: 313: 307: 306: 297: 295: 294: 287: 280: 272: 265: 264: 235:(2): 448–452. 212: 199: 175: 170:Collin College 155: 153: 150: 26: 24: 14: 13: 10: 9: 6: 4: 3: 2: 1053: 1042: 1039: 1037: 1034: 1032: 1029: 1028: 1026: 1015: 1010: 1008: 1003: 1001: 996: 995: 989: 987: 983: 979: 974: 971: 967: 962: 951: 950:Schädler agar 948: 946: 943: 941: 937: 934: 932: 929: 927: 924: 922: 919: 917: 914: 912: 909: 907: 904: 903: 901: 897: 891: 888: 886: 883: 881: 878: 876: 873: 871: 870:Nutrient agar 868: 866: 863: 861: 860:Letheen broth 858: 856: 853: 851: 848: 846: 843: 842: 840: 836: 830: 827: 825: 822: 820: 817: 815: 812: 810: 807: 805: 802: 800: 797: 795: 794:Czapek medium 792: 790: 787: 786: 784: 782: 778: 772: 769: 767: 764: 762: 759: 757: 754: 752: 749: 745: 742: 740: 737: 735: 731: 728: 727: 726: 725: 721: 720: 718: 716: 712: 696: 693: 692: 691: 690: 686: 682: 680: 677: 673: 670: 669: 668: 667: 663: 659: 656: 655: 654: 653: 649: 645: 642: 641: 640: 639: 634: 633: 629: 625: 622: 621: 620: 619: 615: 611: 608: 607: 606: 605: 601: 597: 596: 592: 591: 590: 589: 585: 584: 582: 580: 576: 568: 565: 563: 560: 559: 558: 557: 553: 552: 550: 548: 544: 536: 533: 531: 530:Brucella agar 528: 527: 526: 525: 521: 520: 518: 516: 512: 509: 507: 506:Gram negative 503: 491: 488: 486: 483: 481: 478: 477: 476: 475: 471: 467: 464: 462: 459: 458: 457: 456: 452: 448: 445: 444: 443: 442: 438: 434: 431: 429: 426: 425: 424: 423: 422:Lactobacillus 419: 415: 412: 411: 410: 409: 405: 401: 398: 397: 396: 395: 391: 390: 388: 386: 382: 374: 371: 370: 369: 368: 364: 360: 357: 355: 352: 350: 347: 345: 342: 341: 340: 339: 335: 334: 332: 330: 326: 323: 321: 320:Gram positive 317: 314: 312: 308: 304: 300: 293: 288: 286: 281: 279: 274: 273: 270: 260: 256: 251: 246: 242: 238: 234: 230: 226: 219: 217: 213: 202: 200:0-521-67631-2 196: 192: 188: 187: 179: 176: 171: 167: 164:Cain, Donna. 160: 157: 151: 149: 147: 143: 139: 134: 132: 131: 126: 122: 118: 114: 110: 102: 97: 93: 91: 87: 83: 82:thioglycolate 79: 75: 68: 62: 55: 49: 43: 37: 32: 19: 986:expanding it 978:microbiology 975: 960: 781:Fungal media 722: 683: 664: 650: 636: 630: 616: 602: 593: 586: 554: 522: 472: 461:Fraser broth 453: 439: 420: 408:Enterococcus 406: 400:Hoyle's agar 392: 373:Eaton's agar 365: 336: 299:Growth media 232: 228: 204:. Retrieved 185: 178: 159: 135: 128: 106: 73: 72: 441:Lactococcus 303:agar plates 138:macrophages 130:Clostridium 1025:Categories 845:Blood agar 809:MMN medium 685:Salmonella 666:Salmonella 604:Bordetella 428:Lee's Agar 206:2009-09-14 152:References 90:resazurine 771:TSI slant 744:Endo agar 624:VRBD agar 556:Neisseria 385:Bacillota 101:anaerobes 945:R2A agar 940:PNP agar 789:BAF agar 695:XLD agar 689:Shigella 679:DCA agar 672:XLT agar 524:Brucella 455:Listeria 447:M17 agar 433:MRS agar 36:bacteria 259:6500699 140:to the 466:PALCAM 257:  250:261553 247:  197:  123:, and 976:This 982:stub 824:YEPD 255:PMID 195:ISBN 245:PMC 237:doi 65:5: 59:4: 52:3: 46:2: 40:1: 1027:: 829:YM 301:/ 253:. 243:. 233:46 231:. 227:. 215:^ 189:. 168:. 119:, 115:, 111:, 1013:e 1006:t 999:v 988:. 938:/ 732:/ 687:/ 635:/ 291:e 284:t 277:v 261:. 239:: 209:. 172:. 20:)

Index

Thioglycollate broth

bacteria
Obligate aerobes
Obligate anaerobes
Facultative anaerobes
Microaerophiles
Aerotolerant organisms
differential medium
thioglycolate
obligate anaerobes
resazurine

anaerobes
obligate aerobes
obligate anaerobes
facultative anaerobes
microaerophiles
aerotolerant organisms
Clostridium
macrophages
peritoneal cavity
intraperitoneally
"Fluid Thioglycollate [sic] Broth"
Collin College
District Laboratory Practice in Tropical Countries, Part 2
Cambridge University
ISBN
0-521-67631-2

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