565:"Die chromatographische Adsorptionsanalyse, die in den Händen von M. Tswett) zu wichtigen Resultaten hinsichtlich des Chlorophylls und seiner Derivate geführt hat, halten wir für die Arbeit in größerem Maßstab, also für präparative Zwecke nicht für brauchbar. Die freien Chlorophyllide sind zu unbeständig ; ihre Farbe schlägt leicht in braun um, wenn sie von Adsorbentien aufgenommen werden. Aber selbst die Methylchlorophyllide sind zu empfindlich, so daß es noch weiter Verbesserungen) des Tswettschen Verfahrens bedarf, um sie nach diesem wieder unverändert zu isolieren. Beim Adsorbieren mit Calciumcarbonat und beim Ausziehen mit Alkohol unterliegen alle Chlorophyllide, wie wir in unseren letzten Arbeiten beschreiben haben), leicht der Umwandlung, die sich bei der Verseifung mit Alkalien durch Auftreten schwach basischer Phytochlorine und Phytorhodine verrät. Es ist noch nicht bekannt, ob bei der Tswettschen Adsorptionsanalyse in kleinem Maßstab diese Veränderung der Magnesiumverbindungen vermieden worden ist."
434:"Wird eine petrolätherishe Chlorophyllösung durch eine Säule eines Adsorptionsmittels durchfiltriert (ich verwende hauptsächlich Calciumcarbonate, welches in engen Glasröhren dicht gestampft wird), so werden die Farbstoffe gemäss der Adsorptionsreihe von oben nach unten in verschieden gefärbten Zonen auseinandergelegt, indem die stärker adsorbierten Farbstoffe die schwächer zurückgehaltenen weiter nach unten verdrängen. Diese Trennung wird praktisch vollständig, wenn man nach dem Durchgange der Farbstofflösung durch die adsorbierende Säule einen Strom des reinen Lösungsmittels herstellt. Wie die Lichtstrahlen im Spektrum, so werden in der Calciumkarbonatsäule die verschiedenen Komponenten eines Farbstoffgemisches gesetzmässig auseindergelegt, und lassen sich darin qualitativ und auch quantitativ bestimmen. Ein solches Präparat nenne ich ein Chromatogramm und die entsprechende Methode, die chromatographische Methode."
568:(Chromatographic adsorption analysis, which in the hands of M. Tsvet has led to important results regarding chlorophyll and its derivatives, we hold to be unsuitable for work on a larger scale, thus, for preparative purposes. Free chlorophyllides are too unstable ; their colors turn brown readily when they are adsorbed. But even methylchlorophyllides are too sensitive, so that still further improvements of Tsvet's method are needed in order to isolate them unchanged by this . During adsorption by calcium carbonate and during extraction with alcohol, all chlorophyllides succumb, as we described in our latest articles, readily to the transformation, which betrays itself during hydrolysis with alkalies by the appearance of weakly basic phytochlorine and phytorhodine. It's still not known whether in the case of Tsvet's adsorption analysis ( on a small scale), this change of the magnesium compounds has been avoided.)
440: : If a solution of chlorophyll in petroleum ether is filtered through a column of an adsorbent (I use mainly calcium carbonate, which is tamped firmly in narrow glass tubes), then the pigments are dispersed, according to the adsorption series, from top to bottom in differently coloured zones, by the more strongly adsorbed pigments' displacing farther downwards the more weakly retained ones. This separation is practically complete when one establishes a flow of pure solvent behind the passage of the pigment solution through the adsorbing column. Like light rays in a spectrum, so the different components of a mixture of pigments are dispersed in the calcium carbonate column following a set pattern, and can be determined in there qualitatively as well as quantitatively. I call such a preparation a "chromatogram" and the corresponding method, the "chromatographic method".
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For several reasons, Tsvet's work was long ignored: the violent political upheaval in Russia at the beginning of the 20th century, the fact that Tsvet originally published only in
Russian (making his results largely inaccessible to western scientists), and an article denying Tsvet's findings.
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Tswett, M. S. (1905) "О новой категории адсорбционных явлений и о применении их к биохимическому анализу" (O novoy kategorii adsorbtsionnykh yavleny i o primenenii ikh k biokkhimicheskomu analizu (On a new category of adsorption phenomena and on its application to biochemical analysis)),
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tried to repeat Tsvet's experiments, but because they used an overly aggressive adsorbent (destroying the chlorophyll), were not able to do so. They published their results and Tsvet's chromatography method fell into obscurity. It was revived 10 years after his death thanks to
Austrian
384:(Trudy Varshavskago Obshchestva Estestvoispytatelei, Otdelenie Biologii (Proceedings of the Warsaw Society of Naturalists , Biology Section)), vol. 14, no. 6, pp. 20–39. (Note: Tsvet submitted his manuscript in 1903; however, it was not published until 1905.)
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Kuhn, R. and
Lederer, E. (10 June 1931). "Zerlegung des Carotins in seine Komponenten. (Über das Vitamin des Wachstums, I. Mitteil)" [Separation of carotene into its components. (On the growth vitamin, 1st communication)].
220:. His Geneva degrees were not recognized in Russia, and he had to earn Russian degrees. In 1897 he became a teacher of botany courses for women. In 1902 he became a laboratory assistant at the Institute of Plant Physiology of the
308:. The method was described on 30 December 1901 at the XI Congress of Naturalists and Physicians (XI съезд естествоиспытателей и врачей) in St. Petersburg. The first printed description was in 1905, in the
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Ostrowski, W (1968). "Michael S. Tswett—inventor of column chromatography (On the occasion of 65th anniversary of his lecture on the column chromatography technique)".
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Kuhn, R. and
Lederer, E. (17 February 1931). "Fraktionierung und Isomerisierung des Carotins" [Fractionation and isomerization of carotene].
216:, Russia, in 1896 because his father was recalled from the foreign service. There he started to work at the Biological Laboratory of the
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Kuhn, Richard; Winterstein, Alfred; Lederer, Edgar (1931). "Zur
Kenntnis der Xanthophylle" [On our knowledge of xanthophyll].
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189:, Italy. His mother was Italian, and his father was a Russian official. His mother died soon after his birth, and he was raised in
312:. He first used the term "chromatography" in print in 1906 in his two papers about chlorophyll in the German botanical journal,
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454:[Adsorption analysis and chromatographic method. Application to the chemistry of chlorophyll].
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Livengood, Jonathan (2009). "Why was M. S. Tswett's chromatographic adsorption analysis rejected?".
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Kuhn, Richard and
Lederer, Edgar (1931). "Über α- und β-carotin" [On α- and β-carotene].
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Mikhail Tsvet invented chromatography in 1900 during his research on plant
161:: Михаил Семёнович Цвет; 14 May 1872 – 26 June 1919) was a Russian-Italian
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Berichte der
Deutschen botanischen Gesellschaft 24, 316–323 (1906)
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Proceedings of the Warsaw
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528:[Investigations of chlorophyll: 19. On chlorophyllide].
398:[Physical-chemical studies of chlorophyll. Adsorption].
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526:"Untersuchungen über Chlorophyll: XIX. Über die Chlorophyllid"
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degree from the
Department of Physics and Mathematics at the
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