55:
is an example of a metabolon that facilitates substrate channeling. Another example is the dhurrin synthesis pathway in sorghum, in which the enzymes assemble as a metabolon in lipid membranes. During the functioning of metabolons, the amount of water needed to hydrate the enzymes is reduced and
281:
A – Channeling, B – Specific protein-protein interactions, C – Specific protein – membrane interactions, D – Kinetic effects, E – Multienzyme complexes identified, F – Genetic proofs, G – Operative modeled systems, H – Identified multifunctional proteins, I – Physico-chemical
661:
Robinson, J. B., Jr. & Srere, P. A. (1986) Interactions of sequential metabolic enzymes of the mitochondria: a role in metabolic regulation, pp. 159–171 in
Dynamics of Biochemical Systems (ed. Damjanovich, S., Keleti, T. & Trón, L.), Akadémiai Kiadó, Budapest,
527:
Lyubarev A. E., Kurganov B. I. Supramolecular organisation of
Tricarboxylic Acids Cycle's enzymes. Proceedings of the All-Union Symposium "Molecular mechanisms and regulation of energy metabolism". Puschino, Russia, 1986. p. 13. (in Russian)
482:
Srere P. A. Is there an organization of Krebs cycle enzymes in the mitochondrial matrix? In: Energy
Metabolism and the Regulation of Metabolic Processes in Mitochondria, R. W. Hanson and W.A. Mehlman (Eds.). New York: Academic Press. 1972.
552:
Kurganov B.I., Lyubarev A.E. Enzymes and multienzyme complexes as controllable systems. In: Soviet
Scientific Reviews. Section D. Physicochemical Biology Reviews. V. 8 (ed. V.P. Skulachev). Glasgow, Harwood Acad. Publ., 1988, p. 111-147
366:
Zhang, Youjun; Beard, Katherine F. M.; Swart, Corné; Bergmann, Susan; Krahnert, Ina; Nikoloski, Zoran; Graf, Alexander; Ratcliffe, R. George; Sweetlove, Lee J.; Fernie, Alisdair R.; Obata, Toshihiro (16 May 2017).
540:
Kurganov B. I, Lyubarev A. E. Hypothetical structure of the complex of glycolytic enzymes (glycolytic metabolon), formed on the membrane of erythrocytes. Molek. Biologia. 1988. V.22, No.6, p. 1605–1613. (in
728:
Veliky M.M., Starikovich L. S., Klimishin N. I., Chayka Ya. P. Molecular mechanisms in the integration of metabolism. Lviv
National University Ed., Lviv, Ukraine. 2007. 229 P. (in ukrainian)
672:
Kastritis, Panagiotis L.; O'Reilly, Francis J.; Bock, Thomas; Li, Yuanyue; Rogon, Matt Z.; Buczak, Katarzyna; Romanov, Natalie; Betts, Matthew J.; Bui, Khanh Huy (2017-07-01).
64:
The concept of structural-metabolic cellular complexes was first conceived in 1970 by A. M. Kuzin of the USSR Academy of
Sciences, and adopted in 1972 by Paul A. Srere of the
416:
Laursen, Tomas; Borch, Jonas; Knudsen, Camilla; Bavishi, Krutika; Torta, Federico; Martens, Helle Juel; Silvestro, Daniele; Hatzakis, Nikos S.; Wenk, Markus R. (2016-11-18).
65:
600:
Clarke, F. M.; Stephan, P.; Huxham, G.; Hamilton, D.; Morton, D. J. (1984). "Metabolic dependence of glycolytic enzyme binding in rat and sheep heart".
94:, a complex of a metabolon exists between fatty acid synthase and a MDa carboxylase, and was observed using chemical cross-linking coupled to
733:
80:, Australia also worked on the concept. The name "metabolon" was first proposed in 1985 by Paul Srere during a lecture in Debrecen, Hungary.
417:
76:
enzymes (Embden-Meyerhof-Parnas pathway) by B.I. Kurganov and A.E. Lyubarev. In the mid-1970s, the group of F.M. Clarke at the
565:
Clarke, F. M.; Masters, C. J. (1975). "On the association of glycolytic enzymes with structural proteins of skeletal muscle".
327:"Krebs Cycle Metabolon: Structural Evidence of Substrate Channeling Revealed by Cross-Linking and Mass Spectrometry"
756:
37:
246:
144:
99:
77:
33:
493:
Lyubarev, A. E.; Kurganov, B. I. (1989). "Supramolecular organization of tricarboxylic acid cycle enzymes".
90:
174:
154:
48:
110:
was possible, the metabolon was highly flexible, hindering high-resolution structure determination.
751:
107:
456:
72:. This hypothesis was well accepted in the former USSR and further developed for the complex of
369:"Protein-protein interactions and metabolite channelling in the plant tricarboxylic acid cycle"
729:
711:
693:
617:
582:
510:
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Kuzin A. M. Structural – metabolic hypothesis in radiobiology. Moscow: Nauka Ed., 1970.- 50 p.
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29:
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432:
388:
380:
338:
305:
418:"Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum"
51:
directly into the active site of the next consecutive enzyme of the metabolic pathway. The
295:
134:
47:
The formation of metabolons allows the intermediate product from one enzyme to be passed
706:
673:
613:
393:
368:
745:
648:
578:
506:
674:"Capturing protein communities by structural proteomics in a thermophilic eukaryote"
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300:
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41:
17:
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218:
73:
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715:
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621:
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514:
384:
117:
24:
is a temporary structural-functional complex formed between sequential
25:
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is highly flexible, and although a high-resolution structure of
121:
Metabolic pathways in which formation of metabolons occurs
567:
Biochimica et
Biophysica Acta (BBA) - General Subjects
36:and by structural elements of the cell, such as
325:Wu, Fei; Minteer, Shelley (2 February 2015).
8:
705:
392:
342:
129:Events supporting metabolon's formation
102:. The Fatty acid synthesis metabolon in
331:Angewandte Chemie International Edition
317:
635:Srere, P. A. (1985). "The metabolon".
7:
614:10.1111/j.1432-1033.1984.tb07963.x
14:
602:European Journal of Biochemistry
84:The case of Fatty Acid Synthesis
637:Trends in Biochemical Sciences
56:enzyme activity is increased.
1:
649:10.1016/0968-0004(85)90266-X
579:10.1016/0304-4165(75)90187-7
507:10.1016/0303-2647(89)90038-5
128:
125:
773:
38:integral membrane proteins
678:Molecular Systems Biology
279:
210:Metabolism of amino acids
120:
34:non-covalent interactions
247:Electron transport chain
100:cryo-electron microscopy
78:University of Queensland
32:, held together both by
437:10.1126/science.aag2347
256:Antibiotic biosynthesis
175:Pyrimidine biosynthesis
91:Chaetomium thermophilum
68:for the enzymes of the
344:10.1002/anie.201409336
690:10.15252/msb.20167412
373:Nature Communications
238:Fatty acids oxidation
165:Glycogen biosynthesis
202:Steroid biosynthesis
155:Protein biosynthesis
40:and proteins of the
385:10.1038/ncomms15212
185:Purine biosynthesis
108:Fatty acid synthase
66:University of Texas
194:Lipid biosynthesis
126:Metabolic pathway
98:and visualized by
757:Protein complexes
734:978-966-613-538-7
431:(6314): 890–893.
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286:
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229:Citric acid cycle
96:mass spectrometry
70:citric acid cycle
53:citric acid cycle
30:metabolic pathway
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337:(6): 1851–1854.
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306:Enzyme catalysis
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272:cAMP degradation
145:RNA biosynthesis
135:DNA biosynthesis
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296:Enzyme kinetics
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104:C. thermophilum
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62:
12:
11:
5:
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743:
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721:
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643:(3): 109–110.
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233:B, C, D, E, G
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223:A, B, C, D, I
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159:A, B, C, D, E
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149:A, B, C, E, F
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139:A, B, C, E, F
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61:
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13:
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501:(2): 91–102.
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49:(channelling)
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608:(3): 643–9.
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573:(1): 37–46.
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301:Enzyme assay
103:
89:
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63:
46:
42:cytoskeleton
21:
18:biochemistry
15:
241:A, B, C, D
213:A, B, D, H
197:A, B, C, H
179:A, C, D, F
752:Metabolism
746:Categories
684:(7): 936.
495:Biosystems
312:References
264:Urea cycle
219:Glycolysis
74:glycolytic
698:1744-4292
445:0036-8075
379:: 15212.
22:metabolon
716:28743795
541:Russian)
483:p.79-91.
461:19187608
453:27856908
403:28508886
353:25537779
290:See also
275:A, D, E
205:A, C, E
114:Examples
707:5527848
662:Hungary
622:6692839
587:1111588
515:2720141
425:Science
394:5440813
282:proofs.
60:History
26:enzymes
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457:S2CID
421:(PDF)
267:B, D
259:A, E
251:C, I
189:A, E
169:C, E
28:of a
730:ISBN
712:PMID
694:ISSN
618:PMID
583:PMID
511:PMID
449:PMID
441:ISSN
399:PMID
349:PMID
20:, a
702:PMC
686:doi
645:doi
610:doi
606:138
575:doi
571:381
503:doi
433:doi
429:354
389:PMC
381:doi
339:doi
88:In
16:In
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