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240:. Other protein complexes and higher-order interactions were predicted and validated using a novel integration of experimental and computational methods which include proteomics, cross-linking mass spectrometry, computational structural biology and electron microscopy. The cellular homogenates of
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Cryo-electron microscopic image of fractionated lysate from C. thermophilum cellular extracts. Megadalton protein complexes are observed (e.g. the barrel-shaped fatty acid synthase) that are either co-eluting or interacting to form higher-order protein complex assemblies
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fungi. Studying nuclear pore complex proteins, it was found that protein isolation was more abundant and more soluble than in yeast (yeast proteins precipitate at a lower temperature).
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Bock, Thomas; Chen, Wei-Hua; Ori, Alessandro; Malik, Nayab; Silva-Martin, Noella; Huerta-Cepas, Jaime; Powell, Sean T.; Kastritis, Panagiotis L.; Smyshlyaev, Georgy (2014-12-16).
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Kastritis, Panagiotis L.; O'Reilly, Francis J.; Bock, Thomas; Li, Yuanyue; Rogon, Matt Z.; Buczak, Katarzyna; Romanov, Natalie; Betts, Matthew J.; Bui, Khanh Huy (2017-07-01).
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or compost (rotten organics). It is notable for being a eukaryote with a high temperature tolerance (60 °C). Its optimal growth temperature is 50–55 °C.
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The first observation of a metabolon in fatty acid metabolism at high resolution came from cryo-electron microscopic analysis of cellular homogenates from
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Since fungi are eukaryotic and not distant from animals they are good models for comparative and easy-to-manipulate research, and in the case of
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were used to derive protein complex structures at high resolution, with purity of the native protein complex < 40%.
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Insight into
Structure and Assembly of the Nuclear Pore Complex by Utilizing the Genome of a Eukaryotic Thermophile
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has been studied and 27 distinct protein communities, including 108 interconnected complexes have been identified.
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266:"An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum"
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has been completely sequenced. It spans 28.3 Mb and encodes 7227 predicted protein coding genes.
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are thermophilic and thus better for studies (structural and biochemical) than comparable
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derived from this fungus are heat stable and thus easier to work with. Proteins from
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Model system for identifying higher-order protein interactions
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162:is a thermophilic filamentous
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333:. Genome.fungalgenomics.ca
331:"Genozymes Public Genomes"
360:Molecular Systems Biology
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32:Scientific classification
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209:Saccharomyces cerevisiae
630:Fungi described in 1950
443:Chaetomium thermophilum
416:Chaetomium thermophilum
159:Chaetomium thermophilum
141:Chaetomium thermophilum
25:Chaetomium thermophilum
270:Nucleic Acids Research
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372:10.15252/msb.20167412
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423:Encyclopedia of Life
123:C. thermophilum
282:10.1093/nar/gku1147
276:(22): 13525–13533.
198:Genome and proteome
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597:Open Tree of Life
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16:Species of fungus
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215:The proteome of
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180:C. thermophilum
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202:The genome of
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166:. It grows on
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132:Binomial name
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519:iNaturalist
89:Sordariales
625:Chaetomium
619:Categories
366:(7): 936.
337:2014-05-17
248:References
192:mesophilic
110:Chaetomium
69:Ascomycota
65:Division:
458:Q10447720
380:1744-4292
290:0305-1048
148:La Touche
117:Species:
55:Kingdom:
49:Eukaryota
545:MycoBank
537:10392739
493:Fungorum
452:Wikidata
425:overview
398:28743795
308:25398899
184:proteins
174:Research
95:Family:
45:Domain:
511:2573734
485:1016560
389:5527848
299:4267624
105:Genus:
85:Order:
75:Class:
602:863516
576:209285
550:344053
524:971552
498:344053
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164:fungus
150:(1950)
589:28043
532:IRMNG
59:Fungi
571:NCBI
506:GBIF
472:TD3F
394:PMID
376:ISSN
304:PMID
286:ISSN
168:dung
558:NBN
480:EoL
467:CoL
384:PMC
368:doi
294:PMC
278:doi
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Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
