435:(MTAs) for all materials transferred through Addgene to protect the intellectual property of plasmid depositors. Addgene developed one of the first electronic systems for handling MTAs. By using the standard Universal Biological Material Transfer Agreement (UBMTA) and implementing electronic signatures, Addgene's electronic MTA (eMTA) system expedites the approval process for plasmid orders.
27:
111:
repository. Addgene facilitates the exchange of genetic material between laboratories by offering plasmids and their associated cloning data to non-profit and academic laboratories around the world. Addgene provides a free online database of plasmid cloning information and references, including lists
443:
Addgene won awards for innovation and research including Mass
Nonprofit Network Award for excellence in Innovations, Cambridge award program 2014 Award for Research & Development Laboratories, Mass Technology Leadership Award Finalist 2012.
734:
872:
616:
825:
738:
300:
Addgene collaborates with institutes and consortia to curate plasmid collections for specific purposes. Examples of these collaborations include special collections from the
182:
877:
781:
154:
As of 2014 Addgene's repository comprised 30,000 plasmids, deposited by 1,700 labs. Its plasmid collection contains plasmids used for functions such as
146:
The organization covers the operating costs of maintaining and improving the collection by charging a nominal fee to scientists requesting plasmids.
282:—References to help scientists design plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.
624:
675:
Herscovitch, Melanie; Perkins, Eric; Baltus, Andy; Fan, Melina (10 April 2012). "Addgene provides an open forum for plasmid sharing".
710:
867:
271:—A collection of references for molecular biology reagents, such as primers, restriction enzymes and antibiotic concentrations.
288:—Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by
301:
760:
309:
839:
259:—A curated list of over 4,000 vector backbones, including relevant cloning information and bacterial growth conditions.
461:
432:
415:
265:—An Addgene software tool for creating plasmid maps from sequences with annotated features and restriction sites.
71:
785:
305:
57:
593:
522:
486:
Fan, M; Tsai, J; Chen, B; Fan, K; LaBaer, J (Mar 25, 2005). "A central repository for published plasmids".
229:
834:
188:
862:
289:
384:
211:
155:
654:
692:
574:
503:
117:
684:
564:
495:
399:
368:
95:
419:
411:
380:
340:
159:
407:
395:
372:
360:
348:
856:
847:
391:
364:
344:
137:
499:
403:
356:
285:
376:
352:
336:
316:
26:
224:
113:
578:
279:
268:
829:
696:
507:
262:
735:"mass non-profit, nonprofit award 2013 excellence in innovation finalists"
552:
256:
320:
143:
Addgene accepts plasmids from researchers for distribution and archival.
128:
Addgene was founded in 2004 by Melina Fan, Kenneth Fan, and Benjie Chen.
108:
688:
177:
569:
842:
711:"mass nonprofit network nominates addgene for an excellence award"
312:. The plasmids are available to both academic and industry labs.
167:
163:
236:
304:, Zinc Finger Consortium, the Cell Migration Consortium, the
646:
811:
170:
and promoter activity. The plasmid collection includes:
166:
knockdown, viral-mediated gene delivery, detection of
89:
81:
64:
53:
41:
33:
617:"Addgene partners with Michael J. Fox Foundation"
873:Non-profit organizations based in Massachusetts
390:8 Breakthrough Prize in Life Sciences winners;
183:Transcription Activator-Like Effector Nuclease
8:
19:
112:of commonly used vector backbones, popular
18:
16:Non-profit plasmid repository organization
568:
594:"Repositories share key research tools"
553:"Repositories share key research tools"
452:
427:Electronic Material Transfer Agreements
315:In 2020, Addgene received funding from
319:to subsidize the cost of reagents for
136:Addgene's headquarters are located in
7:
878:2004 establishments in Massachusetts
615:Resende, Patricia (3 October 2012).
14:
761:"cambridge recognition awarding"
25:
657:from the original on 2021-12-23
331:Noteworthy depositors include:
782:"Mass Tech announces finalist"
500:10.1126/science.307.5717.1877a
302:Structural Genomics Consortium
292:and bacterial transformation.
1:
310:The Michael J. Fox Foundation
433:Material Transfer Agreements
60:, biological resource center
551:Baker, Monya (2014-01-01).
286:Molecular Cloning Protocols
269:Molecular Biology Reference
199:Species-specific expression
894:
820:Other plasmid repositories
460:Scudellari, Megan (2012).
24:
335:13 Nobel Prize winners;
280:Molecular Cloning Guides
138:Watertown, Massachusetts
72:Watertown, Massachusetts
868:Biotechnology databases
252:Molecular biology tools
58:Non-profit organization
275:Plasmid Cloning Guides
221:Retroviral/Lentiviral
158:(including CRISPRS),
677:Nature Biotechnology
189:Zinc finger nuclease
98:, Executive director
523:"Got any plasmids?"
462:"Sharing Made Easy"
290:gel electrophoresis
174:Genome engineering
21:
521:Kamens, J (2012).
385:Johann Deisenhofer
212:Fluorescent marker
208:Selectable markers
156:genome engineering
150:Plasmid repository
85:Plasmid repository
627:on 9 October 2012
563:(7483): 272–272.
431:Addgene requires
263:Sequence Analyzer
118:molecular cloning
102:
101:
885:
815:
814:
812:Official website
798:
797:
795:
793:
788:on 17 April 2014
784:. Archived from
778:
772:
771:
769:
767:
757:
751:
750:
748:
746:
737:. Archived from
731:
725:
724:
722:
720:
715:
707:
701:
700:
689:10.1038/nbt.2177
672:
666:
665:
663:
662:
643:
637:
636:
634:
632:
623:. Archived from
612:
606:
605:
603:
601:
589:
583:
582:
572:
548:
542:
541:
539:
537:
532:. pp. 58–59
527:
518:
512:
511:
483:
477:
476:
474:
472:
457:
400:Lewis C. Cantley
369:Robert Lefkowitz
247:Tools and guides
242:shRNA expression
196:Empty backbones
107:is a non-profit
96:Chonnettia Jones
29:
22:
893:
892:
888:
887:
886:
884:
883:
882:
853:
852:
848:Harvard PlasmID
822:
810:
809:
806:
801:
791:
789:
780:
779:
775:
765:
763:
759:
758:
754:
744:
742:
733:
732:
728:
718:
716:
713:
709:
708:
704:
674:
673:
669:
660:
658:
645:
644:
640:
630:
628:
614:
613:
609:
599:
597:
591:
590:
586:
570:10.1038/505272a
550:
549:
545:
535:
533:
525:
520:
519:
515:
485:
484:
480:
470:
468:
459:
458:
454:
450:
441:
429:
420:Shinya Yamanaka
416:Robert Weinberg
412:Bert Vogelstein
381:Jennifer Doudna
341:Shinya Yamanaka
329:
308:collection and
298:
257:Vector Database
249:
205:Fusion proteins
160:gene expression
152:
134:
126:
92:
77:
48:
46:
17:
12:
11:
5:
891:
889:
881:
880:
875:
870:
865:
855:
854:
851:
850:
845:
837:
832:
821:
818:
817:
816:
805:
804:External links
802:
800:
799:
773:
752:
726:
702:
683:(4): 316–317.
667:
638:
621:Mass High Tech
607:
592:Baker, Monya.
584:
543:
513:
494:(5717): 1877.
478:
451:
449:
446:
440:
437:
428:
425:
424:
423:
408:Titia de Lange
396:David Botstein
388:
373:Martin Chalfie
361:Eric Wieschaus
349:Mario Capecchi
328:
325:
297:
296:Collaborations
294:
248:
245:
244:
243:
240:
234:
233:
232:
227:
222:
218:Viral vectors
216:
215:
214:
209:
206:
203:
200:
194:
193:
192:
186:
180:
151:
148:
133:
130:
125:
122:
116:plasmids, and
100:
99:
93:
90:
87:
86:
83:
79:
78:
76:
75:
68:
66:
62:
61:
55:
51:
50:
43:
39:
38:
35:
31:
30:
15:
13:
10:
9:
6:
4:
3:
2:
890:
879:
876:
874:
871:
869:
866:
864:
861:
860:
858:
849:
846:
844:
841:
838:
836:
835:BIOSS Toolbox
833:
831:
827:
824:
823:
819:
813:
808:
807:
803:
787:
783:
777:
774:
762:
756:
753:
741:on 2013-09-02
740:
736:
730:
727:
712:
706:
703:
698:
694:
690:
686:
682:
678:
671:
668:
656:
652:
648:
647:"Fast Grants"
642:
639:
626:
622:
618:
611:
608:
595:
588:
585:
580:
576:
571:
566:
562:
558:
554:
547:
544:
531:
524:
517:
514:
509:
505:
501:
497:
493:
489:
482:
479:
467:
466:The Scientist
463:
456:
453:
447:
445:
438:
436:
434:
426:
421:
417:
413:
409:
405:
401:
397:
393:
392:Cori Bargmann
389:
386:
382:
378:
374:
370:
366:
365:Phillip Sharp
362:
358:
354:
350:
346:
345:Bruce Beutler
342:
338:
334:
333:
332:
326:
324:
322:
318:
313:
311:
307:
303:
295:
293:
291:
287:
283:
281:
277:
276:
272:
270:
266:
264:
260:
258:
254:
253:
246:
241:
238:
235:
231:
228:
226:
223:
220:
219:
217:
213:
210:
207:
204:
201:
198:
197:
195:
190:
187:
184:
181:
179:
176:
175:
173:
172:
171:
169:
165:
161:
157:
149:
147:
144:
141:
139:
131:
129:
123:
121:
119:
115:
110:
106:
97:
94:
88:
84:
80:
73:
70:
69:
67:
63:
59:
56:
52:
44:
40:
36:
32:
28:
23:
790:. Retrieved
786:the original
776:
764:. Retrieved
755:
743:. Retrieved
739:the original
729:
717:. Retrieved
705:
680:
676:
670:
659:. Retrieved
650:
641:
629:. Retrieved
625:the original
620:
610:
598:. Retrieved
596:. Nature.com
587:
560:
556:
546:
534:. Retrieved
529:
516:
491:
487:
481:
469:. Retrieved
465:
455:
442:
430:
404:Hans Clevers
357:Richard Axel
330:
314:
299:
284:
278:
274:
273:
267:
261:
255:
251:
250:
202:Epitope tags
185:(TALEN) kits
153:
145:
142:
135:
127:
104:
103:
47:Kenneth Fan
45:Benjie Chen
651:Fast Grants
631:21 November
536:21 November
471:21 November
377:Roger Tsien
353:Andrew Fire
337:John Gurdon
317:Fast Grants
120:protocols.
863:Gene banks
857:Categories
661:2023-05-21
448:References
327:Depositors
323:research.
239:expression
225:Adenoviral
132:Operations
114:lentiviral
91:Key people
49:Melina Fan
579:1476-4687
530:Lab Times
697:22491276
655:Archived
508:15790830
321:COVID-19
82:Services
65:Location
488:Science
178:CRISPRs
124:History
109:plasmid
105:Addgene
42:Founder
34:Founded
20:Addgene
695:
577:
557:Nature
506:
439:Awards
383:, and
843:DNASU
792:9 May
766:9 May
745:9 May
719:9 May
714:(PDF)
600:8 May
526:(PDF)
168:miRNA
164:shRNA
74:, USA
830:LMBP
826:BCCM
794:2014
768:2014
747:2014
721:2014
693:PMID
633:2012
602:2014
575:ISSN
538:2012
504:PMID
473:2012
418:and
237:cDNA
191:kits
54:Type
37:2004
840:PSI
685:doi
565:doi
561:505
496:doi
492:307
306:KLF
230:AAV
859::
691:.
681:30
679:.
653:.
649:.
619:.
573:.
559:.
555:.
528:.
502:.
490:.
464:.
414:,
410:,
406:,
402:,
398:,
394:,
379:,
375:,
371:,
367:,
363:,
359:,
355:,
351:,
347:,
343:,
339:,
162:,
140:.
828:/
796:.
770:.
749:.
723:.
699:.
687::
664:.
635:.
604:.
581:.
567::
540:.
510:.
498::
475:.
422:.
387:.
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
