17:
339:
222:
As mentioned above, in order for APC to be useful in immunoassays it must first be chemically cross-linked to prevent it from dissociating into its component subunits when in common physiological buffers. The conventional method for accomplishing this is through a destructive process wherein the
144:
Allophycocyanin can be isolated from various species of red or blue-green algae, each producing slightly different forms of the molecule. It is composed of two different subunits (α and β) in which each subunit has one phycocyanobilin (PCB) chromophore. The subunit structure for APC has been
223:
treated APC trimer is chemically disrupted in 8M urea and then allowed to re-associate through in a physiological buffer. An alternative method can be used which preserves the structural integrity of the APC trimer and allows for a brighter, more stable end-product.
337:, Morseman, John Peter; Moss, Mark Wesley & Allnutt, F C Thomas, "High fluorescent intensity cross-linked allophycocyanin", published 2007-08-14, assigned to Columbia Biosciences Corp.
268:
Papageorgiou GC, Lagoyanni T (September 1983). "Effects of chaotropic electrolytes on the structure and electronic excitation coupling of glutaraldehyde-and diimido ester-cross-linked phycobilisomes".
463:
248:
371:
297:
Yeh SW, Ong LJ, Clark JH, Glazer AN (January 1987). "Fluorescence properties of allophycocyanin and a crosslinked allophycocyanin trimer".
236:
129:
606:
626:
616:
270:
364:
621:
108:. Allophycocyanin absorbs and emits red light (650 and 660 nm max, respectively), and is readily found in
357:
231:
Many applications and instruments were developed specifically for
Allophycocyanin. It is commonly used in
334:
468:
611:
575:
473:
381:
89:
316:
100:. All phycobiliproteins are water-soluble and therefore cannot exist within the membrane like
93:
26:
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308:
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113:
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125:
42:
600:
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109:
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85:
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128:, it is often abbreviated APC. To be effectively used in applications such as
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133:
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117:
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349:
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580:
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104:, but aggregate, forming clusters that adhere to the membrane called
120:. Phycobilin pigments have fluorescent properties that are used in
15:
353:
16:
249:
Time
Resolved-Fluorescence Resonance Energy Transfer (TR-FRET)
65:
56:
47:
553:
525:
487:
454:
411:
388:
271:
Biochimica et
Biophysica Acta (BBA) - Bioenergetics
243:, and High Throughput Screening as an acceptor for
149:. The molecular weight of APC is 105,000 daltons.
464:1,7-Bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one
365:
8:
136:, APC needs to be chemically cross-linked.
372:
358:
350:
156:
260:
132:, High-Throughput Screening (HTS) and
7:
41:("other algal blue protein"; from
14:
1:
284:10.1016/0005-2728(83)90091-9
20:Allophycocyanin dodekamer,
645:
168:Additional Absorption peak
140:Structural characteristics
76:from the light-harvesting
66:
57:
48:
153:Spectral characteristics
607:Photosynthetic pigments
627:Cyanobacteria proteins
313:10.1002/cyto.990080113
192:Extinction Coefficient
35:
335:US patent 7256050
72:meaning "blue") is a
22:Gloeobacter violaceus
19:
617:Fluorescent proteins
469:Bisdemethoxycurcumin
63:meaning “alga”, and
80:family, along with
622:Bacterial proteins
576:Phycoerythrocyanin
474:Desmethoxycurcumin
160:Absorption maximum
145:determined as (αβ)
90:phycoerythrocyanin
36:
594:
593:
517:Condensed tannins
215:
214:
94:accessory pigment
54:meaning "other",
634:
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367:
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218:Cross-linked APC
176:Emission maximum
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114:blue-green algae
78:phycobiliprotein
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51:
50:
33:
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561:Allophycocyanin
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39:Allophycocyanin
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502:Anthocyanidins
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382:plant pigments
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241:flow cytometry
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179:657.5 nm
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126:flow cytometry
106:phycobilisomes
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2:
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571:Phycoerythrin
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507:Anthoxanthins
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441:Chlorophyll f
439:
437:
436:Chlorophyll d
434:
432:
431:Chlorophyll c
429:
427:
426:Chlorophyll b
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422:
421:Chlorophyll a
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278:(3): 323–32.
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226:
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211:1.6 x 10 Mcm
210:
207:
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200:Quantum Yield
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112:(also called
111:
110:Cyanobacteria
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86:phycoerythrin
83:
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71:
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53:
44:
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28:
23:
18:
560:
545:Xanthophylls
497:Anthocyanins
456:Curcuminoids
403:Betaxanthins
329:
304:
298:
292:
275:
269:
263:
233:immunoassays
230:
227:Applications
221:
217:
216:
195:700,000 Mcm
187:5.5 nm
184:Stokes Shift
171:625 nm
163:652 nm
143:
64:
55:
46:
38:
37:
21:
586:Xanthonoids
566:Phycocyanin
527:Carotenoids
413:Chlorophyll
398:Betacyanins
307:(1): 91–5.
122:immunoassay
102:carotenoids
98:chlorophyll
92:. It is an
82:phycocyanin
601:Categories
489:Flavonoids
255:References
208:Brightness
134:microscopy
612:Red algae
540:Retinoids
535:Carotenes
390:Betalains
380:Types of
300:Cytometry
124:kits. In
118:red algae
581:Quinones
479:Curcumin
251:assays.
245:Europium
235:such as
70:(kyanos)
61:(phykos)
34:)
512:Flavans
446:Chlorin
321:3100257
116:), and
74:protein
52:(allos)
341:
319:
67:κυανός
554:Other
203:0.68
58:φύκος
49:ἄλλος
43:Greek
317:PMID
247:via
237:FACS
130:FACS
88:and
31:2vjt
309:doi
280:doi
276:724
96:to
27:PDB
603::
315:.
303:.
274:.
239:,
84:,
45::
29::
373:e
366:t
359:v
323:.
311::
305:8
286:.
282::
147:3
24:(
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