233:(observed in mice model after intranasal infection) but without any remarkable influence on the host immune response. That was one of the reasons this gene was targeted during search for more safe and efficient vaccine against
269:
Kettle S, Blake NW, Law KM, Smith GL (January 1995). "Vaccinia virus serpins B13R (SPI-2) and B22R (SPI-1) encode M(r) 38.5 and 40K, intracellular polypeptides that do not affect virus virulence in a murine intranasal model".
307:"Vaccinia virus serpin B13R (SPI-2) inhibits interleukin-1beta-converting enzyme and protects virus-infected cells from TNF- and Fas-mediated apoptosis, but does not prevent IL-1beta-induced fever"
792:
787:
844:
1372:
1367:
1362:
1357:
1319:
1314:
1694:
1352:
1347:
823:
818:
813:
33:
537:
2075:
252:
Vaccinia virus encodes two more serpin - SPI-1 and SPI-3. SPI-1 influences the host range and SPI-3 stops infected cells from fusing together.
2209:
1838:
444:"Vaccinia viruses with a serpin gene deletion and expressing IFN-gamma induce potent immune responses without detectable replication in vivo"
501:
650:
241:
leads to strong attenuation but without decreasing host immune response. Host reaction was comparable to that on MVA virus (modified
1808:
1742:
969:
1436:
1104:
342:
Law RH, Zhang Q, McGowan S, Buckle AM, Silverman GA, Wong W, Rosado CJ, Langendorf CG, Pike RN, Bird PI, Whisstock JC (2006).
1955:
1429:
1424:
530:
2057:
1992:
1664:
1640:
1447:
1332:
1238:
1164:
1089:
984:
868:
803:
723:
689:
640:
223:
733:
660:
625:
1615:
1419:
620:
2114:
2002:
1928:
1866:
1818:
1770:
1722:
1674:
1553:
1402:
1297:
1058:
964:
944:
927:
777:
665:
600:
214:(or ICE – interleukin-1 converting enzyme) which is under normal circumstances activated by the formation of
523:
145:
767:
2177:
2013:
1898:
738:
2147:
2038:
1918:
1790:
1650:
1949:
1887:
1574:
1018:
1009:
931:
878:
590:
455:
1620:
1014:
1004:
923:
157:
242:
2142:
2019:
203:
149:
249:
nowadays. The
Copenhagen strain of vaccinia virus only has a truncated version of this protein.
219:
237:. It was shown that immunization with vaccinia virus with deleted SPI-2 and 1 and coexpressing
1594:
1342:
1281:
1046:
483:
424:
375:
324:
287:
192:
126:
1856:
1462:
1199:
1194:
1189:
1184:
1179:
1174:
1153:
473:
463:
414:
406:
365:
355:
314:
279:
246:
238:
172:
2086:
1543:
1533:
1286:
130:
73:
459:
2160:
2043:
1588:
1413:
1308:
914:
834:
419:
394:
370:
343:
107:
478:
443:
283:
2203:
2109:
1760:
1221:
858:
575:
550:
546:
114:
1829:
1781:
1733:
1685:
1606:
1391:
1227:
904:
713:
580:
554:
215:
196:
188:
161:
515:
410:
2169:
1712:
1032:
995:
83:
319:
306:
2051:
1983:
1580:
1386:
1217:
1213:
49:
442:
Legrand FA, Verardi PH, Chan KS, Peng Y, Jones LA, Yilma TD (February 2005).
2097:
2092:
2033:
2027:
1271:
1148:
1138:
1126:
899:
563:
468:
360:
211:
180:
175:(serpins). Serpins are the most broadly distributed family of inhibitors of
153:
141:
487:
428:
379:
328:
291:
679:
234:
207:
176:
54:
305:
Kettle S, AlcamĂ A, Khanna A, Ehret R, Jassoy C, Smith GL (March 1997).
230:
137:
103:
1939:
1877:
1625:
1564:
1457:
955:
611:
184:
122:
218:. SPI-2 expression also inhibits the apoptotic pathway activated by
1510:
1500:
1495:
1485:
1480:
1475:
1470:
1077:
1072:
1067:
2062:
1893:
1505:
1490:
919:
655:
165:
118:
519:
2123:
2119:
2102:
1248:
1099:
140:
with size of 38,5 kDa. It is expressed in an early phase of
125:. About one third of the genome is not necessary for the
191:
where they serve as inhibitors of proteases involved in
202:
SPI-2 inhibits processing of an inactive precursor of
148:
was identified about 72 nucleotides upstream from the
129:
itself. These viral products interfere with the host
2168:
2159:
2136:
2073:
2000:
1991:
1982:
1938:
1926:
1917:
1876:
1864:
1855:
1828:
1816:
1807:
1780:
1768:
1759:
1732:
1720:
1711:
1684:
1672:
1663:
1638:
1605:
1563:
1551:
1542:
1527:
1445:
1400:
1385:
1330:
1295:
1280:
1265:
1236:
1212:
1162:
1147:
1134:
1125:
1087:
1056:
1045:
994:
982:
954:
942:
913:
895:
866:
857:
832:
801:
775:
766:
755:
721:
712:
687:
678:
638:
610:
598:
589:
571:
562:
79:
69:
64:
48:
40:
28:
23:
18:
171:SPI-2 belongs in superfamily of the inhibitors of
133:. SPI-2 is one of these immunomodulatory factors.
395:"Understanding the mechanism of IL-1β secretion"
117:family. These viruses contain approximately 200
531:
502:"family:"serpin family poxviruses subfamily""
229:Deletion of SPI-2 leads to virus attenuation
164:modifier of the cytokine response – known as
8:
179:. They were identified in all multicellular
2165:
2156:
1997:
1988:
1935:
1923:
1873:
1861:
1825:
1813:
1777:
1765:
1729:
1717:
1681:
1669:
1560:
1548:
1539:
1397:
1292:
1277:
1233:
1159:
1144:
1131:
1053:
991:
951:
939:
910:
863:
772:
763:
718:
684:
607:
595:
586:
568:
538:
524:
516:
393:Lopez-Castejon G, Brough D (August 2011).
156:of the host cell. SPI-2 shares 92% of its
61:
1695:Parainfluenza hemagglutinin-neuraminidase
477:
467:
418:
369:
359:
318:
344:"An overview of the serpin superfamily"
261:
34:Vaccinia virus (strain Western Reserve)
152:. The translated protein stays in the
15:
1839:Respiratory syncytial virus G protein
7:
206:(pro-IL-1β) to active form of this
14:
1743:Mumps hemagglutinin-neuraminidase
1105:Hepatitis B virus DNA polymerase
936:(non-enveloped circular ds-DNA)
1:
1956:Vesiculovirus matrix proteins
411:10.1016/j.cytogfr.2011.10.001
284:10.1016/S0042-6822(95)80028-X
2210:Viral nonstructural proteins
448:Proc. Natl. Acad. Sci. U.S.A
210:. It does so by binding to
113:Vaccinia virus is member of
1993:Structure and genome of HIV
1050:(circular partially ds-DNA)
199:and complement activation.
136:SPI-2 is a nonglycosylated
2226:
626:Herpesvirus glycoprotein B
399:Cytokine Growth Factor Rev
320:10.1099/0022-1317-78-3-677
313:. 78 ( Pt 3) (3): 677–85.
144:. The initiation site for
621:HHV capsid portal protein
60:
187:serpins are secreted in
1616:Influenza hemagglutinin
469:10.1073/pnas.0409846102
361:10.1186/gb-2006-7-5-216
2178:Gag-onc fusion protein
2148:murine leukemia virus
2039:Reverse transcriptase
1919:Indiana vesiculovirus
1791:Measles hemagglutinin
879:Early 35 kDa protein
245:) which serves as a
181:eukaryotic organisms
19:B13R (virus protein)
460:2005PNAS..102.2940L
158:amino acid sequence
2143:Rous sarcoma virus
150:open reading frame
98:(sometimes called
2197:
2196:
2193:
2192:
2189:
2188:
2185:
2184:
2132:
2131:
1978:
1977:
1974:
1973:
1970:
1969:
1966:
1965:
1913:
1912:
1909:
1908:
1851:
1850:
1847:
1846:
1803:
1802:
1799:
1798:
1755:
1754:
1751:
1750:
1707:
1706:
1703:
1702:
1659:
1658:
1634:
1633:
1523:
1522:
1519:
1518:
1381:
1380:
1261:
1260:
1257:
1256:
1208:
1207:
1121:
1120:
1117:
1116:
1113:
1112:
1041:
1040:
1028:
1027:
978:
977:
891:
890:
887:
886:
853:
852:
751:
750:
747:
746:
708:
707:
674:
673:
634:
633:
193:blood coagulation
127:viral replication
93:
92:
89:
88:
2217:
2166:
2157:
1998:
1989:
1936:
1924:
1874:
1862:
1857:Zaire ebolavirus
1826:
1814:
1778:
1766:
1730:
1718:
1682:
1670:
1561:
1549:
1540:
1463:3C-like protease
1398:
1293:
1278:
1234:
1160:
1154:Duplornaviricota
1145:
1132:
1054:
992:
952:
940:
911:
864:
773:
764:
719:
685:
608:
596:
587:
569:
540:
533:
526:
517:
510:
509:
498:
492:
491:
481:
471:
439:
433:
432:
422:
390:
384:
383:
373:
363:
339:
333:
332:
322:
302:
296:
295:
266:
247:smallpox vaccine
173:serine proteases
62:
36:
16:
2225:
2224:
2220:
2219:
2218:
2216:
2215:
2214:
2200:
2199:
2198:
2181:
2128:
2087:transactivators
2069:
1962:
1905:
1843:
1795:
1747:
1699:
1655:
1630:
1601:
1544:Influenza virus
1534:Negarnaviricota
1531:
1529:
1515:
1441:
1389:
1377:
1326:
1287:Kitrinoviricota
1284:
1269:
1267:
1253:
1225:
1204:
1151:
1136:
1109:
1083:
1049:
1037:
1024:
974:
935:
917:
903:
897:
883:
849:
828:
797:
759:
758:(Duplodnaviria,
757:
756:circular ds-DNA
743:
704:
670:
630:
579:
573:
558:
544:
514:
513:
500:
499:
495:
441:
440:
436:
392:
391:
387:
341:
340:
336:
304:
303:
299:
268:
267:
263:
258:
131:immune response
32:
12:
11:
5:
2223:
2221:
2213:
2212:
2202:
2201:
2195:
2194:
2191:
2190:
2187:
2186:
2183:
2182:
2180:
2175:
2173:
2163:
2161:Fusion protein
2154:
2153:
2152:
2151:
2150:
2145:
2134:
2133:
2130:
2129:
2127:
2126:
2117:
2112:
2107:
2106:
2105:
2100:
2095:
2082:
2080:
2071:
2070:
2068:
2067:
2066:
2065:
2060:
2048:
2047:
2046:
2044:HIV-1 protease
2041:
2036:
2024:
2023:
2022:
2009:
2007:
1995:
1986:
1980:
1979:
1976:
1975:
1972:
1971:
1968:
1967:
1964:
1963:
1961:
1960:
1959:
1958:
1950:matrix protein
1945:
1943:
1933:
1921:
1915:
1914:
1911:
1910:
1907:
1906:
1904:
1903:
1902:
1901:
1896:
1888:matrix protein
1883:
1881:
1871:
1859:
1853:
1852:
1849:
1848:
1845:
1844:
1842:
1841:
1835:
1833:
1823:
1811:
1805:
1804:
1801:
1800:
1797:
1796:
1794:
1793:
1787:
1785:
1775:
1763:
1757:
1756:
1753:
1752:
1749:
1748:
1746:
1745:
1739:
1737:
1727:
1715:
1709:
1708:
1705:
1704:
1701:
1700:
1698:
1697:
1691:
1689:
1679:
1667:
1661:
1660:
1657:
1656:
1654:
1653:
1647:
1645:
1636:
1635:
1632:
1631:
1629:
1628:
1623:
1618:
1612:
1610:
1603:
1602:
1600:
1599:
1598:
1597:
1589:viral envelope
1585:
1584:
1583:
1575:matrix protein
1570:
1568:
1558:
1546:
1537:
1530:negative-sense
1525:
1524:
1521:
1520:
1517:
1516:
1514:
1513:
1508:
1503:
1498:
1493:
1488:
1483:
1478:
1473:
1468:
1467:
1466:
1454:
1452:
1443:
1442:
1440:
1439:
1434:
1433:
1432:
1427:
1422:
1414:viral envelope
1409:
1407:
1395:
1383:
1382:
1379:
1378:
1376:
1375:
1370:
1365:
1360:
1355:
1350:
1345:
1339:
1337:
1328:
1327:
1325:
1324:
1323:
1322:
1317:
1309:viral envelope
1304:
1302:
1290:
1275:
1268:positive-sense
1263:
1262:
1259:
1258:
1255:
1254:
1252:
1251:
1245:
1243:
1231:
1210:
1209:
1206:
1205:
1203:
1202:
1197:
1192:
1187:
1182:
1177:
1171:
1169:
1157:
1142:
1129:
1123:
1122:
1119:
1118:
1115:
1114:
1111:
1110:
1108:
1107:
1102:
1096:
1094:
1085:
1084:
1082:
1081:
1080:
1070:
1065:
1063:
1051:
1043:
1042:
1039:
1038:
1036:
1035:
1029:
1026:
1025:
1023:
1022:
1012:
1007:
1001:
999:
989:
980:
979:
976:
975:
973:
972:
967:
961:
959:
949:
937:
915:Polyomaviridae
908:
893:
892:
889:
888:
885:
884:
882:
881:
875:
873:
861:
855:
854:
851:
850:
848:
847:
841:
839:
830:
829:
827:
826:
821:
816:
810:
808:
799:
798:
796:
795:
790:
784:
782:
770:
761:
760:Varidnaviria?)
753:
752:
749:
748:
745:
744:
742:
741:
736:
730:
728:
716:
710:
709:
706:
705:
703:
702:
696:
694:
682:
676:
675:
672:
671:
669:
668:
663:
658:
653:
647:
645:
636:
635:
632:
631:
629:
628:
623:
617:
615:
605:
593:
591:Herpes simplex
584:
566:
560:
559:
547:Viral proteins
545:
543:
542:
535:
528:
520:
512:
511:
493:
434:
385:
334:
297:
260:
259:
257:
254:
204:interleukin-1β
108:vaccinia virus
91:
90:
87:
86:
81:
77:
76:
71:
67:
66:
58:
57:
52:
46:
45:
42:
38:
37:
30:
26:
25:
21:
20:
13:
10:
9:
6:
4:
3:
2:
2222:
2211:
2208:
2207:
2205:
2179:
2176:
2174:
2171:
2167:
2164:
2162:
2158:
2155:
2149:
2146:
2144:
2141:
2140:
2139:
2138:
2135:
2125:
2121:
2118:
2116:
2113:
2111:
2108:
2104:
2101:
2099:
2096:
2094:
2091:
2090:
2089:
2088:
2084:
2083:
2081:
2079:
2077:
2072:
2064:
2061:
2059:
2056:
2055:
2054:
2053:
2049:
2045:
2042:
2040:
2037:
2035:
2032:
2031:
2030:
2029:
2025:
2021:
2018:
2017:
2016:
2015:
2011:
2010:
2008:
2006:
2004:
1999:
1996:
1994:
1990:
1987:
1985:
1981:
1957:
1954:
1953:
1952:
1951:
1947:
1946:
1944:
1941:
1937:
1934:
1932:
1930:
1925:
1922:
1920:
1916:
1900:
1897:
1895:
1892:
1891:
1890:
1889:
1885:
1884:
1882:
1879:
1875:
1872:
1870:
1868:
1863:
1860:
1858:
1854:
1840:
1837:
1836:
1834:
1831:
1827:
1824:
1822:
1820:
1815:
1812:
1810:
1806:
1792:
1789:
1788:
1786:
1783:
1779:
1776:
1774:
1772:
1767:
1764:
1762:
1758:
1744:
1741:
1740:
1738:
1735:
1731:
1728:
1726:
1724:
1719:
1716:
1714:
1710:
1696:
1693:
1692:
1690:
1687:
1683:
1680:
1678:
1676:
1671:
1668:
1666:
1665:Parainfluenza
1662:
1652:
1649:
1648:
1646:
1644:
1642:
1637:
1627:
1624:
1622:
1621:Neuraminidase
1619:
1617:
1614:
1613:
1611:
1608:
1604:
1596:
1593:
1592:
1591:
1590:
1586:
1582:
1579:
1578:
1577:
1576:
1572:
1571:
1569:
1566:
1562:
1559:
1557:
1555:
1550:
1547:
1545:
1541:
1538:
1535:
1526:
1512:
1509:
1507:
1504:
1502:
1499:
1497:
1494:
1492:
1489:
1487:
1484:
1482:
1479:
1477:
1474:
1472:
1469:
1464:
1461:
1460:
1459:
1456:
1455:
1453:
1451:
1449:
1444:
1438:
1435:
1431:
1428:
1426:
1423:
1421:
1418:
1417:
1416:
1415:
1411:
1410:
1408:
1406:
1404:
1399:
1396:
1393:
1388:
1384:
1374:
1371:
1369:
1366:
1364:
1361:
1359:
1356:
1354:
1351:
1349:
1346:
1344:
1341:
1340:
1338:
1336:
1334:
1329:
1321:
1318:
1316:
1313:
1312:
1311:
1310:
1306:
1305:
1303:
1301:
1299:
1294:
1291:
1288:
1283:
1279:
1276:
1273:
1264:
1250:
1247:
1246:
1244:
1242:
1240:
1235:
1232:
1229:
1223:
1219:
1215:
1211:
1201:
1198:
1196:
1193:
1191:
1188:
1186:
1183:
1181:
1178:
1176:
1173:
1172:
1170:
1168:
1166:
1161:
1158:
1155:
1150:
1146:
1143:
1140:
1133:
1130:
1128:
1124:
1106:
1103:
1101:
1098:
1097:
1095:
1093:
1091:
1086:
1079:
1076:
1075:
1074:
1071:
1069:
1066:
1064:
1062:
1060:
1055:
1052:
1048:
1044:
1034:
1031:
1030:
1020:
1016:
1013:
1011:
1008:
1006:
1003:
1002:
1000:
997:
993:
990:
988:
986:
981:
971:
968:
966:
963:
962:
960:
957:
953:
950:
948:
946:
941:
938:
933:
929:
925:
921:
916:
912:
909:
906:
901:
894:
880:
877:
876:
874:
872:
870:
865:
862:
860:
859:Baculoviridae
856:
846:
843:
842:
840:
838:
836:
831:
825:
822:
820:
817:
815:
812:
811:
809:
807:
805:
800:
794:
791:
789:
786:
785:
783:
781:
779:
774:
771:
769:
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762:
754:
740:
737:
735:
732:
731:
729:
727:
725:
720:
717:
715:
711:
701:
698:
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695:
693:
691:
686:
683:
681:
677:
667:
664:
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652:
649:
648:
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644:
642:
637:
627:
624:
622:
619:
618:
616:
613:
609:
606:
604:
602:
597:
594:
592:
588:
585:
582:
577:
576:Duplodnaviria
572:linear ds-DNA
570:
567:
565:
561:
556:
552:
548:
541:
536:
534:
529:
527:
522:
521:
518:
507:
503:
497:
494:
489:
485:
480:
475:
470:
465:
461:
457:
454:(8): 2940–5.
453:
449:
445:
438:
435:
430:
426:
421:
416:
412:
408:
405:(4): 189–95.
404:
400:
396:
389:
386:
381:
377:
372:
367:
362:
357:
353:
349:
345:
338:
335:
330:
326:
321:
316:
312:
311:J. Gen. Virol
308:
301:
298:
293:
289:
285:
281:
278:(1): 136–47.
277:
273:
265:
262:
255:
253:
250:
248:
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236:
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227:
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169:
167:
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159:
155:
151:
147:
146:transcription
143:
139:
134:
132:
128:
124:
120:
116:
115:Orthopoxvirus
111:
109:
106:expressed by
105:
101:
97:
85:
82:
78:
75:
72:
68:
63:
59:
56:
53:
51:
47:
43:
39:
35:
31:
27:
22:
17:
2085:
2074:
2050:
2026:
2012:
2001:
1948:
1927:
1886:
1865:
1830:glycoprotein
1817:
1782:glycoprotein
1769:
1734:glycoprotein
1721:
1686:glycoprotein
1673:
1639:
1607:glycoprotein
1587:
1573:
1552:
1446:
1437:Nucleocapsid
1412:
1401:
1392:Pisuviricota
1331:
1307:
1296:
1237:
1228:Pisuviricota
1163:
1088:
1057:
983:
943:
905:Monodnaviria
867:
833:
802:
776:
768:Epstein–Barr
722:
714:Adenoviridae
699:
688:
639:
599:
581:Varidnaviria
505:
496:
451:
447:
437:
402:
398:
388:
351:
347:
337:
310:
300:
275:
271:
264:
251:
243:virus Ankara
228:
216:inflammasome
201:
197:inflammation
170:
162:cowpox virus
135:
112:
99:
95:
94:
2170:oncoprotein
1282:Hepatitis C
1047:Hepatitis B
1033:Agnoprotein
996:oncoprotein
970:VP2 and VP3
348:Genome Biol
74:Swiss-model
24:Identifiers
1595:M2 protein
1581:M1 protein
1387:SARS-CoV-2
354:(5): 216.
256:References
220:Fas-ligand
70:Structures
65:Search for
2137:Multiple
2034:Integrase
1272:Riboviria
1149:Rotavirus
1139:Riboviria
900:Riboviria
212:caspase 1
177:proteases
160:with the
154:cytoplasm
142:infection
121:in their
2204:Category
1430:Membrane
1425:Envelope
1019:SV40 Tag
680:Vaccinia
488:15705716
429:22019906
380:16737556
272:Virology
235:smallpox
208:cytokine
84:InterPro
29:Organism
1761:Measles
1214:Rhinov.
661:ICP34.5
506:UniProt
456:Bibcode
420:3714593
371:1779521
329:9049422
292:7831769
231:in vivo
185:mammals
138:peptide
104:protein
102:) is a
80:Domains
50:UniProt
1940:capsid
1878:capsid
1626:HA-tag
1565:capsid
1528:ss-RNA
1458:ORF1ab
1266:ss-RNA
1226:etc. (
1135:ds-RNA
956:capsid
824:EBNA-3
819:EBNA-2
814:EBNA-1
612:capsid
486:
479:548597
476:
427:
417:
378:
368:
327:
290:
189:plasma
123:genome
55:P15059
41:Symbol
2076:VRAPs
2058:gp120
1713:Mumps
1511:ORF9b
1501:ORF7b
1496:ORF7a
1486:ORF3d
1481:ORF3c
1476:ORF3b
1471:ORF3a
1465:(NS5)
1420:Spike
1222:Hep A
1218:Polio
1078:HBeAg
1073:HBcAg
1068:HBsAg
932:HaPyV
928:MCPyV
896:other
835:ncRNA
793:LMP-2
788:LMP-1
666:ICP47
651:vmw65
551:early
183:. In
119:genes
100:SPI-2
2063:gp41
2003:VSPs
1929:VSPs
1899:VP24
1894:VP40
1867:VSPs
1819:VSPs
1771:VSPs
1723:VSPs
1675:VSPs
1641:VNPs
1554:VSPs
1506:ORF8
1491:ORF6
1448:VNPs
1403:VSPs
1373:NS5B
1368:NS5A
1363:NS4B
1358:NS4A
1333:VNPs
1298:VSPs
1239:VNPs
1200:NSP6
1195:NSP5
1190:NSP4
1185:NSP3
1180:NSP2
1175:NSP1
1165:VNPs
1090:VNPs
1059:VSPs
1015:LTag
1010:MTag
1005:STag
985:VNPs
945:VSPs
924:MPyV
920:SV40
869:VNPs
845:EBER
804:VNPs
778:VSPs
724:VNPs
700:B13R
690:VNPs
656:ICP8
641:VNPs
601:VSPs
555:late
553:and
484:PMID
425:PMID
376:PMID
325:PMID
288:PMID
239:IFNÎł
224:TNFα
222:and
166:crmA
96:B13R
2124:Vpx
2122:or
2120:Vpu
2115:Vif
2110:Nef
2103:Vpr
2098:Rev
2093:Tat
2052:env
2028:pol
2020:p24
2014:gag
1809:RSV
1651:NS1
1353:NS3
1348:NS2
1249:VPg
1127:RNA
1100:HBx
965:VP1
739:E1B
734:E1A
564:DNA
474:PMC
464:doi
452:102
415:PMC
407:doi
366:PMC
356:doi
315:doi
280:doi
276:206
2206::
1984:RT
1343:P7
1320:E2
1315:E1
1220:,
1216:,
930:,
926:,
922:,
504:.
482:.
472:.
462:.
450:.
446:.
423:.
413:.
403:22
401:.
397:.
374:.
364:.
350:.
346:.
323:.
309:.
286:.
274:.
226:.
195:,
168:.
110:.
2172::
2078::
2005::
1942::
1931::
1880::
1869::
1832::
1821::
1784::
1773::
1736::
1725::
1688::
1677::
1643::
1609::
1567::
1556::
1536:)
1532:(
1450::
1405::
1394:)
1390:(
1335::
1300::
1289:)
1285:(
1274:)
1270:(
1241::
1230:)
1224:,
1167::
1156:)
1152:(
1141:)
1137:(
1092::
1061::
1021:)
1017:(
998::
987::
958::
947::
934:)
918:(
907:)
902:,
898:(
871::
837::
806::
780::
726::
692::
643::
614::
603::
583:)
578:,
574:(
557:)
549:(
539:e
532:t
525:v
508:.
490:.
466::
458::
431:.
409::
382:.
358::
352:7
331:.
317::
294:.
282::
44:?
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