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processing is offset against the volume of effluent that can be sterilised, as the steam takes up space that could be used to hold effluent. Steam injection is a very noisy process – steam rushing through the effluent can sound like a jet engine. The process can also cause solid material to stick to the sides of the sterilisation tank, which can hinder heat transference from the walls of a jacketed vessel. Low temperature and pressure variants of batch steam injection EDS have been shown capable of decontaminating biosafety level 2 waste by subjecting it to a sterilisation temperature of 82.2 °C. This low temperature requires a long time period of six hours to achieve sterilisation.
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is passed through the cavity in the walls of jacketed vessel, raising its temperature of over 121 °C. Once all the effluent has been heated to at least 121 °C for at least 30 minutes, all biologically hazardous material within the kill tank will have been sterilised. At this point, the tank
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Batch steam injection systems function similarly to a batch steam EDS, but steam is passed directly through the effluent during the sterilisation stage. This procedure increases the speed at which the effluent can reach the required sterilisation temperature, increasing the throughput time. Speed of
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Continuous flow EDS pass liquid effluent through a distance of heated pipework to sterilise it. The heated pipework is frequently coiled to minimise heat loss and the space required. The length and bore of the heated pipework can vary greatly, depending on the rate of flow of the effluent and the
107:
While EDS are designed chiefly to sterilise liquid waste, they can in some instances sterilise solid material carried by the liquid effluent. However the EDS may require grinders to break down the solid materials before they enter site of sterilisation in the EDS, and macerating paddles to stir
282:
Chmielewski, Revis; Day, Michael; Spatz, Stephen; Yu, Qingzhong; Gast, Richard; Zsak, Laslo; Swayne, David (1 December 2011). "Thermal
Inactivation of Avian Viral and Bacterial Pathogens in an Effluent Treatment System within a Biosafety Level 2 and 3 Enhanced Facility".
563:
Chmielewski, Revis; Day, Michael; Spatz, Stephen; Yu, Qingzhong; Gast, Richard; Zsak, Laslo; Swayne, David (December 2011). "Thermal
Inactivation of Avian Viral and Bacterial Pathogens in an Effluent Treatment System within a Biosafety Level 2 and 3 Enhanced Facility".
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Cote, Christopher K.; Weidner, Jessica M.; Klimko, Christopher; Piper, Ashley E.; Miller, Jeremy A.; Hunter, Melissa; Shoe, Jennifer L.; Hoover, Jennifer C.; Sauerbry, Brian R.; Buhr, Tony; Bozue, Joel A.; Harbourt, David E.; Glass, Pamela J. (9 July 2020).
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in the effluent have been sterilised. After sterilisation, the sterilant must be neutralised before the effluent can discarded into a sewer. As such, a batch chemical EDS requires a large quantity of chemicals, both sterilants and neutralisers, to operate
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For smaller scale systems of less than 100 gallons a day, chemical effluent decontamination systems can be used. Effluent is collected in a sterilisation tank, where it is mixed with a chemical sterilant such as
135:, which is a container with hollow walls. Effluent flows into the kill tank either by gravity or through being pumped into the tank. Once the tank is full of effluent, high-temperature pressurised
634:
Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent
Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
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Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent
Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
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Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent
Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
493:
Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent
Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
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Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent
Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
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Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent
Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
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Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent
Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
353:
Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent
Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
318:
Trembalay, Gilles; Langer-Curry, Rebecca; Chris, Kiley; Cory, Ziegler (2010). "Effluent Decontamination Systems: Addressing the Challenges of Planning, Designing, Testing, and Validation".
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temperature that the pipework is heated to. As a hotter temperature sterilises faster, the hotter the temperature of the pipework, the higher the flow rate of the continuous flow EDS.
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can be deactivated in a bleach-based chemical EDS effectively at a sterilant concentration of less than 5700 part per million over two hours of exposure
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A Batch EDS consists of at least one sterilisation tank (also known as a kill tank, or a cook tank). The sterilisation tank is commonly a
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Daugelat, Sabine; Phyu, Sabai; Taillens, Charles; Wee, Hooi Leong; Mattila, Juha; Nurminen, Teppo; McDonnell, Gerald (1 June 2008).
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While a batch EDS must have at least one sterilisation tank, multiple sterilisation tanks can be used and fed from dedicated
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147:. It is noteworthy that when a kill tank is not running, it can function like a storage tank – collecting effluent and
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Effluent Decontamination Systems are an essential feature of Biosafety Level 4 laboratories.
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236:"The Design and Testing of a Continuous Effluent Sterilization System for Liquid Waste"
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672:"Biological Validation of a Chemical Effluent Decontamination System"
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EDS vary in their design and function, however the use of either
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within effluent containing a mixture of animal effluent,
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mixture are then held for sufficient time to ensure all
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Batch steam injection effluent decontamination system
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may be emptied by gravity or by fluid displacement.
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76:facilities. In fact, all facilities in the
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96:animal rooms in laboratory environments.
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20:A batch Effluent Decontamination System
151:until it is full enough to sterilise.
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108:effluent held in tanks, reducing
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80:that produce liquid waste of
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78:United States of America
40:biologically active or
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724:Laboratory equipment
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215:fetal bovine serum
202:Studies show that
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175:electricity
173:, steam or
171:Natural gas
123:is common.
110:congelation
90:waste water
52:, research
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697:12 October
267:12 October
221:References
211:humic acid
149:wastewater
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192:sterilant
46:hospitals
38:sterilise
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204:Bacillus
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511:S2CID
476:S2CID
441:S2CID
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137:steam
92:from
699:2020
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