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then is the process of removing analytes from the adsorbent by running a solvent, called an "eluent", past the adsorbent–analyte complex. As the solvent molecules "elute", or travel down through the chromatography column, they can either pass by the adsorbent–analyte complex or displace the analyte
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by binding to the adsorbent in its place. After the solvent molecules displace the analyte, the analyte can be carried out of the column for analysis. This is why as the mobile phase, called an "eluate", passes out of the column, it typically flows into a
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is the volume of eluent required to cause elution. Under standard conditions for a known mix of solutes in a certain technique, the elution volume may be enough information to identify solutes. For instance, a mixture of
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off the adsorbent to which it is attached. This often happens when the eluent adsorbs onto the stationary phase, displacing the analyte. Such series are useful for determining necessary solvents needed for
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in a liquid chromatography column. The adsorbent, a solid phase, called a "stationary phase", is a powder which is coated onto a solid support. Based on an adsorbent's composition, it can have varying
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of a solute is the time between the start of the separation (the time at which the solute enters the column) and the time at which the solute elutes. In the same way, the
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340:. Techniques include using heat, a freeze-thaw cycle, ultrasound, acids or organic solvents. No single method is best in all situations.
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is the process of extracting one material from another by washing with a solvent: washing of loaded
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Predicting and controlling the order of elution is a key aspect of column chromatographic and
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is listing of various compounds in order of eluting power for a given
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or is collected by a fraction collector for compositional analysis.
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Eluting power (least eluting power → greatest eluting power)
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is largely a measure of how well the solvent can "pull" an
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as well as on the compound used to determine the order.
393:. International Union of Pure and Applied Chemistry.
27:Extraction of a material by washing with a solvent
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41:Elution principle of column chromatography
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367:in high performance liquid chromatography
178:Unsaturated hydrocarbons; alkenyl halides
95:is generally adsorbed by ("bound to") an
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276:, the eluent is the liquid solvent; in
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175:Saturated hydrocarbons; alkyl halides
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450:American Medical Technologists (AMT)
181:Aromatic hydrocarbons; aryl halides
443:"Elution Techniques in Blood Bank"
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304:Elution time and elution volume
296:material that emerges from the
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184:Polyhalogenated hydrocarbons
152:, to polar solvents such as
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135:. The "eluting power" of a
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478:Advances in chromatography
441:George H. Roberts (2006).
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391:"IUPAC Gold Book: eluent"
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231:Ethyl acetate (anhydrous)
199:Acids and bases (amines)
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30:Not to be confused with
476:Brown, Phillis (2001).
400:10.1351/goldbook.E02040
422:Cite journal requires
117:column electrophoretic
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274:liquid chromatography
193:Aldehydes and ketones
82:chromatography column
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533:Analytical chemistry
321:may be separated by
234:Acetone (anhydrous)
61:to remove captured
59:ion-exchange resins
516:2015-12-22 at the
506:Chemistry glossary
278:gas chromatography
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511:Eluotropic series
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228:Ether (anhydrous)
213:Hexane or pentane
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129:eluotropic series
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87:In a liquid
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319:amino acids
246:Acetic acid
216:Cyclohexane
71:biopolymers
53:chemistry,
32:elutriation
527:Categories
463:2020-07-22
406:2008-09-28
377:References
355:Desorption
225:Chloroform
101:affinities
47:analytical
482:CRC Press
133:adsorbent
119:methods.
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69:or other
514:Archived
371:Leaching
344:See also
243:Pyridine
237:Methanol
196:Alcohols
154:methanol
150:n-hexane
110:detector
67:proteins
294:analyte
240:Ethanol
219:Benzene
141:analyte
137:solvent
105:Elution
93:analyte
73:from a
55:elution
51:organic
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290:eluate
284:Eluate
266:eluant
262:eluent
256:Eluent
249:Water
190:Esters
187:Ethers
457:(PDF)
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308:The "
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158:water
18:Elute
486:ISBN
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